• Title/Summary/Keyword: ebb & flow culture

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Effects of Culture Type and Inoculation Quantity in Bioreactor on Production of Potato Plantlets

  • Choi Ki Young;Son Sung Ho;Lee Joo Hyun;Lee Yong-Beom;Bae Jong Hyang
    • Journal of Bio-Environment Control
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    • v.14 no.4
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    • pp.298-301
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    • 2005
  • Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

Effects of Ebb-and-flow System with Double-tier Bench on Growth and Yield of Hydroponically Grown Gymnocalycium mihanovichii 'I-Hong' (2단 벤치를 활용한 담배수 관수 시스템이 수경재배 비모란선인장 '이홍'의 생장 및 수량에 미치는 영향)

  • Ki Young Park;Jung-Soo Lee
    • Journal of Bio-Environment Control
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    • v.32 no.2
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    • pp.132-138
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    • 2023
  • The Gymnocalycium mihanovichii has been an important export item of Korean flower industry for a long time. Although there is a high demand for grafted cactus from overseas, its production for export is limited. In this study, the growth and marketable yield characteristics of Gymnocalycium mihanovichii 'I-Hong' were compared between soil culture and ebb-and-flow hydroponic system with single- or double-tier bench. As a result, hydroponic methods with single-tier bench resulted in higher fresh weight and glove diameter compared to other cultivation methods. In the ebb-and-flow hydroponic system, hydroponic system with double-tier bench of grafted cactus traits has a lower growth rate than other cultivations. However, the hydroponic system with double-tier bench of grafted cactus significantly increased the yield. In conclusion, the yield from hydroponic system with double-tier bench was better than soil cultivation method. Although there were some differences in color depending on the cultivation method, it was considered that there was no difference in appearance of Gymnocalycium mihanovichii 'I-Hong'. Our results suggest the cultivation methods to overcome production constraints, expand their exports, and improve the value-added characteristics of grafted cactus.

Optimum Media of Sedum kamtschaticum for Pot Culture in an Ebb & Flow System (Ebb & Flow 시스템에서 기린초 분화재배에 적합한 배지 선발)

  • Kim, Su Jeong;Chang, Dong Chil;Suh, Jong Taek;Lee, Eung Ho
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.205-210
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    • 2008
  • Sedum kamtschaticum has been used as one of the native valuable plants for ornamental purpose. This experiment was conducted to find out the optimum media for pot cultured Sedum kamtschaticum using peatmoss and coir-based mixtures. Four mixtures such as peatmoss:perlite(5:5, v/v), peatmoss:perlite(7:3, v/v), coir:perlite(5:5, v/v), and coir:perlite(7:3, v/v) were used. Plants were irrigated with a Sonneveld solution in an ebb & flow system. The content of T-N, K, and Ca in the peatmoss-based media was higher than those of the coir-based media. The concentration of T-N, K, and Ca in the shoots of Sedum kamtschaticum increased when plants were cultured with peatmoss-based media. In the peatmoss-based media, plant growth, such as number of branches, leaf length and shoot fresh and dry weights, were significantly increased than those of plant grown in the coir-based media. As the results, the optimum media for pot cultured Sedum kamtschaticum in an ebb & flow system was considered to be the peatmoss-based mixtures.

Practical Use of Nutrient-flowing Wick Culture System(NFW) for Potted Plant Production (양액 흘림식 심지이용형 분화생산시스템(NFW)의 실용화)

  • 손정익;정동호;노은희;오명민;김성규;김기선;손기철
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2003.04a
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    • pp.112-118
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    • 2003
  • 분화 양액 재배에 있어서 저면 관수 방식은 두상 관수 방식 보다 더 널리 사용되고 있다. 저면 관수 방식의 장점으로는 지상부에 물이 닿지 않아 고품질의 식물체 생산 가능, 균일한 크기의 식물체 생산 가능, 물과 양분의 사용의 줄임 등이 있다 (Newman, 1999). 현재 유럽은 저면 관수방식의 대표적인 ebb-and-flow 시스템이 상용화 되어있으며, 북미에서도 점점 그 사용 비율이 증가하고 있다 (Biernbaum, 1990). (중략)

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Influence of Dam Water Discharge on the Oceanography and Fishery (해황과 어황에 미치는 댐 방수의 방향)

  • Chang Sun-duck
    • 한국해양학회지
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    • v.1
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    • pp.49-55
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    • 1971
  • After the heavy precipitation from April 1969 to September 1970, more than 1.359 10$\^$6/㎥ of fresh water was discharged through diversion channel of the Namgang Dam. The sands and muds in the northern Sachon Bay were moved and swept away by the strong southsard flow which was observed to be speedier than five knots. The shellfish culture facility as well as the oysters and bivalves were buried. In Sachon Bay, a southward surface ebb current of approximately one knot stratifies above the northward flow of approximately 0.5 knot, which seems to be similar to the salt wedge estuary. The stratified current is responsible for the breaking of the gill nets and other fishing gears. The salinity of sea water in Chinju Bay decreased remarkably and the abnormal low salinity water lower than 5 was distributed in Sachon Bay. The low salinity water front was observed in eastern Chinju Bay and the Samchonpo Channel, where the salinity was increased from 4.6 to approximately 30 within half a tidal cycle. These caused the oysters and bivalves die, and drove anchovies and octopus out to the sea and prevented them from approaching the bay. The decrease of salinity causes the decrease of density and osmotic pressure of sea water. Turbid water would prevent the sunlight from penetrate into deep layer.

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Increased Growth by LED and Accumulation of Functional Materials by Florescence Lamps in a Hydroponics Culture System for Angelica gigas (당귀의 수경재배에서 LED 광원에 의한 생장 증가와 형광등에 의한 기능성물질 축적)

  • Lee, Gong-In;Kim, Hong-Ju;Kim, Sung-Jin;Lee, Jong-Won;Park, Jong-Seok
    • Journal of Bio-Environment Control
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    • v.25 no.1
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    • pp.42-48
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    • 2016
  • Angelica gigas, belonging family Apiaceae, is a perennial and famous medical plant growing in Korea, Japan, and China. The aims of this study was to analyze the growth and accumulated Decursin and its precursor Decursinol angelate of A.gigas grown under fluorescent lamp and LED. A. gigas 'Manchu' were sowed and managed for seedlings stage in a glass house for 4 weeks. One hundred twenty seedlings with 3 true leafs were transplanted at an ebb & flow system with fluorescent lamp and LED [red: peak wavelength 660nm, blue: peak wavelength 455 nm, white = 3:2:4 ratio] irradiated at $180{\pm}7{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at the top of plant canopy for 5 weeks. The number of leaves increased by 13.5% in the LED treatment, though it is not statistically significant. Leaf length/width ratio of A. gigas grown under the fluorescent lamps was 24% bigger than the LED treatment and also the stem was 13% larger. Maximum root length was similar to both groups. Fresh weight and dry weight of shoots grown under the LED increased by 50% and 42% and the both weights of roots increased by 125% and 45%, respectively. The contents of Decursin and Decursinol angelate grown under the florescent lamps were larger than LED by 188% and 27% in shoot and 78% and 8% in root. The contents of Decursin and Decursinol angelate per plant grown under LED and florescent lamps were 132mg and 122mg. In conclusion, functional materials in A. gigas were increased by florescent light and its growth was promoted by LEDs light.