• Korean Journal of Soil Science and Fertilizer
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• v.49 no.1
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• pp.75-80
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• 2016

This study was conducted to evaluate effect of chemical amendments on reducing bioavailable fraction of heavy metals in soil along with ecotoxicological effect on earthworms, Eisenia fetida. Three different chemical amendments, lime (L), steel slag (SS), and acid mine drainage sludge (AMDS), were applied with varied application ratio (1, 3, 5%). Heavy metal contaminated soil was mixed with chemical amedments and earthworms, Eisenia fetida, were cultivated for 28 days. Bioavailable fraction of heavy metals (Cd, Cu, Pb, and Zn) extracted with 0.1N HCl was monitored and also, mortality, growth, and metal concentration in earthworm were assessed. Result showed that all three amendments had high efficiency to reduce bioavailable fraction of heavy metals in soil. In particular, lime showed the highest reduction rate of Cu (63.9-87.7%), Pb (7.90-24.65%), and Zn (40.83-77.60%) among three amendments. No mortality of earthworm was observed during experimental period except 3% and 5% AMDS treatment indicating that application of chemical amendments is safe in terms of ecotoxicological aspect. However, no positive correlation was observed between reduction of bioavaialble fraction of heavy metals in soil and earthworms. Overall, application of chemical amendments in agricultural field can be adapted for reducing bioavailable fraction of heavy metals and detoxification in soil.

• Hwankyungkyoyuk
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• v.19 no.2 s.30
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• pp.147-158
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• 2006

The purpose of this study is to closely examine the changes of food and drink waste amounts, and student's environmental literacy. By using creative methods, discretional activity classes, and programs to dispose of food and drink waste from students schoolmeals, and at the same time conduct and teach environmental education classes. To achieve this, students were given ten class lessons in creative discretional activities. Students were given flowerpots containing earthworms and were instructed to raise them, using food and drink waste from school meals. Four classes who were participating in project were examined for changes from March to July 2005. As a result the environmental education classes through creative discretional activities didn't have any significant influence on the students environmental literacy. However it did make a difference to the student's in-terms of their ecological knowledge. The project using earthworms to dispose of food and waste drink showed a decrease of 15% of the amount of waste food and drink from school meals. Moreover student's environmental literacy had been significantly influenced about environmental sensibility, value and causes. Through this study I found that the project using earthworms to dispose of waste food and drink, and creative discretional activities as a lesson decreased the amount of food and drink waste at the school, and the changes to students concerns and understanding about environmental sensibility, value and the various causes of waste food and drink.

• Proceedings of the KAIS Fall Conference
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• 2004.06a
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• pp.311-314
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• 2004

Toxicity test fur various synthetic lubricant oil bases derived from biodiesel was carried out. The earthworms(Eisenia fetida) were maintanied in artificial soil consisting of sand for 14 days. Eisenia fetida was cultivated at temperature of $20{\pm}2^{\circ}C$, pH 6.0 $\pm$ 0.5 and moisture of $31\~37\%$. Pentaerythritol(PE) lubricant oil base was tested for acute toxicities to Eisenia fetida. The earthworms were exposed to each lubricant oil with various concentrations of lubricant oil base. After 14 days, the number of surviving earthworms and thier weight alteration during the test period was determined. $LC_{50}$(lethal concentration when $50\%$ of the population were killed) pentaerythritol was determined to be 1,555 mg/kg soil dry weight.

• Korean Journal of Veterinary Research
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• v.15 no.1
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• pp.51-55
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• 1975

In this study two sets of experiments were undertaken. Firstly, the embryonated eggs and first-stage larvae, sprayed in disinfected soil, were exposed to different temperatures. Then, the viabilities of them were weekly determined by inoculating the soil to earthworms, Eisenia foetida. Secondly, the infective larvae, seperated from the earthworms and suspended in tap water, were also exposed to different temperatures and their viabilities were checked microscopically at weekly intervals. The results were summerized as follows: 1. The maximum longevities of embryonated eggs and first-stage larvae were determined as 1 week at $35^{\circ}C$, over 36 weeks at $25^{\circ}C$, $15^{\circ}C$, and $5^{\circ}C$, 32 weeks at $-5^{\circ}C$, and under 1 week at $-15^{\circ}C$. 2. The mean numbers of infective larvae detected from the test earthworms were greatest at $5^{\circ}C$, and decreased with rise or fall of the temperature. 3. Infective larvae freed from the intermediate host were able to survive for 2 weeks at $25^{\circ}C$ and 3 weeks at $15^{\circ}C$. However, they lost their viabilities in a week at $35^{\circ}C$, $5^{\circ}C$, $-5^{\circ}C$, and $-15^{\circ}C$. 4. The number of living infective larvae at $15^{\circ}C$ was greater than at $25^{\circ}C$.

• Journal of the Korea Organic Resources Recycling Association
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• v.12 no.3
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• pp.112-118
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• 2004

Laying hens' excrement from eating food wastes was mixed with paper mill sludge, aged for 21 days and then provided to the juvenile earthworms(Eisenia fetida) for 10 weeks. Biomass of earthworm population decreased by 5.7% of initially introduced population. Very few juvenile earthworms developed into the clitellates and clitellated earthworms could not produce cocoons at all, which was supposed to be caused by inhibition effects of salts in laying hens' excrement upon the sexual development of Eisenia fetida. But there was no significant effect on the survivorship of earthworm population.

• The Korean Journal of Soil Zoology
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• v.5 no.2
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• pp.125-131
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• 2000

Disposing technique for dairy cattle manure using earthworms and the possibility of utilizing earthworms in poultry feed were investigated. In addition, chemical properties of manure and cast of earthworms were investigated. Temperature in the vinyl-house for earthworm nursery was able to be maintained above 0$\^{C}$ in winter and 25-28$\^{C}$ in summer. In closed-covering method, the number of escaped earthworms was the highest among three covering methods. The time was longer after manure excretion, the numbers of escaped earthworm were higher. There were some incidences of mortality of earthworm in closed-covering site. The amount of manure ingested by earthworm was increased in order of non-covering, closed-covering and gap-covering method. Fresh manure was more ingested than old one by earthworm. Earthworms reduced manure about 50% on the dry weight base and reduced total amounts of N, P$_2$O$\_$5/ and K$_2$O of manure by 41, 50 and 60%, respectively. Earthworm could be utilized by adult poultry (>60 g) for feeding. However, adverse effect of live earthworm was observed with young poultry (<450 g).

• Journal of the Korea Organic Resources Recycling Association
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• v.22 no.1
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• pp.57-66
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• 2014

The present study was conducted to provide basic data for environmentally friendly treatment of manure using vermicomposting. This was done by investigating the influence of physiochemical property change during the composting period on the survival of earthworms after mixing cow manure in different levels (0(CRH0), 10(CRH10), 20(CRH20), 30(CRH30), and 40%(CRH40)) with the rice hull that is generally added for composting manure. As composting proceeds, earthworms were able to survive in all conditions after 3 weeks. In terms of the C/N ratio by treatment groups, the rice hull mix treatments were significantly higher than the CRH0 treatment. Among rice hull mix treatments, the treatments with 30~40% rice hull level (CRH30 & CRH40) showed the highest ratio out of all composting periods (p<0.05). The C/N ratio in the 3rdweek when earthworms started surviving was 23.26~34.44. As composting progressed, pH and electrolytic conductivity (EC) were the highest in the CRH0 treatment (p<0.05) and tended to decrease with higher proportion of rice hull in the mix. It was found that pH and electrolytic conductivity (EC) that earthworms start to survive are 7.58~7.74 and 0.41~1.17 mS/cm, respectively. To summarize, when composing cow manure with various levels of rice hull mix, all physiochemical property changes turned out to allow the survival of earthworms, but the results suggest that efficient vermicomposting requires the tests to examine the growth and reproduction according to the rice hull mix ratio.

• Korean Journal of Organic Agriculture
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• v.22 no.1
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• pp.197-208
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• 2014

The present study was conducted to provide basic data for efficient vermicomposting of swine manure by investigating the effect of sawdust mix on growth and reproduction of earthworms, amount of earthworm cast produced, and its chemical composition when mixing swine manure in different levels (0(SSD0), 10(SSD10), 20(SSD20), 30(SSD30), and 40%(SSD40)) with sawdust commonly used as moist control in manure composting. The C/N ratio of the feed and the survival rate of earthworms for the SSD0 treatment (10.19, 35.2%), The C/N ratio of the feed was 11.09-16.44 and increased with higher level of sawdust mixed, and the survival rate of earthworms during the experiment period was 35.2-100%. The number of earthworms was 16.6 in the SSD40 treatment, and young worms were not found in the SSD0 treatment or the treatment with less than 30% sawdust in the mix. The number and the weight of cocoons in SSD30 and SSD40 treatments were found to be in the ranges of 23.8-59.8 and 256-660 mg respectively. The production of earthworm cast and digested amount were higher with the higher level of mixed sawdust (p<0.05), and the C/N ratio of cast increased with the mixed sawdust ratio, being significantly higher ratio in SSD30 and SSD40 than other treatments (p<0.05). Heavy metals content tended to decrease with the mixed sawdust level. To summarize, the higher level of mixed sawdust was associated with improvement in growth and reproduction of earthworms, and 30-40% sawdust mix in particular could result in efficient vermicomposting.

• Korean Journal of Organic Agriculture
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• v.22 no.1
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• pp.183-195
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• 2014

The present study was conducted to provide basic data for environmentally friendly treatment of manure using vermicomposting by investigating the influence of physiochemical property change during the composting period on the survival of earthworms after mixing cow manure in different levels (0(CSD0), 10(CSD10), 20(CSD20), 30(CSD30), and 40%(CSD40)) with the sawdust that is used bedding in livestock pens. As composting proceeds, earthworms were able to survive in all treatments after 3 weeks. In terms of the C/N ratio by treatment groups, the sawdust mix treatment was significantly higher than the CSD0 treatment (p<0.05). The C/N ratio in the 3rdweek when earthworms started surviving was 23.26-61.05. As composting progressed, pH and electrolytic conductivity were highest in the CSD0 treatment and tended to decrease with higher proportion of sawdust in the mix. It was found that pH and electrolytic conductivity that earthworms starting to survive are 7.4-7.7 and 0.28-1.17 mS/cm respectively. To summarize, when composing cow manure with various levels of sawdust mix, all physiochemical property changes turned out to allow the survival of earthworms, but the results suggest that efficient vermicomposting requires the tests to examine growth and reproduction according to the sawdust mix ratio.

• The Korean Journal of Soil Zoology
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• v.7 no.1_2
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• pp.17-21
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• 2002

Food waste which had been mixed with night soil sludge and composted for 21 days (mixing ratio = food waste/paper mill sludge : 1, w/w) was supplied to the 5 g of Eisenia fetida popualtion at various temperatures (5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 31, 32, 33, 34, 35$^{\circ}C$). The biomass of earthworms one month after feeding at 20-32$^{\circ}C$ was about two times more than that of the introduced earthworms, increasing rate of which was much higher than them at the other tested temperatures . There were decreases at temperatures below 7$^{\circ}C$, and less increases at temperatures of 8-10$^{\circ}C$ in biomass. Earthworms could not survive at temperatures at 34$^{\circ}C$ and 35$^{\circ}C$. Feeding rates of earthworms were much higher at 20-32$^{\circ}C$ than them at the other tested temperatures, and there were no differences among them at temperatures of 20-32$^{\circ}C$. But at 3 months after feeding, the biomass and feeding rate of earthworms decreased severely even at 20$^{\circ}C$ and 30$^{\circ}C$, which was supposed to be due to the long term effect of salts, especially sodium chloride (NaCl) in the food wastes.