• Title/Summary/Keyword: eMP

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The Interaction of Mastoparan B from Venom of a Hornet Vespa Basalis with Phospholipid Matrices

  • 박남규;Yuhji Yamato;Sannamu Lee;Gohsuke Sugihara;박장수;강신원
    • Bulletin of the Korean Chemical Society
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    • v.17 no.3
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    • pp.239-244
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    • 1996
  • Mastoparan B (MP-B) that is a novel MP isolated from the hornet Vespa basalis, was studied as compared with MP, in terms of interaction with phospholipid bilayer and antimicrobial activity. MP-B has more hydrophilic amino acid residues in hydrophilic face of amphiphilic α-helical structure than MP. The both peptides exhibited considerably different effect on interaction with lipid bilayers, e.g. their conformation in the presence of acidic and neutral liposomes, dye-release ability from encapsulated liposomes, but on the whole the interaction mode was similar. On antimicrobial activity, MP had a strong activity against Gram-positive bacteria but no against Gram negative ones. Contrary to this, MP-B had a strong activity against Gram-positive and potent against Gram-negative ones. Since both peptides have almost same residues on the hydrophobic side, such more hydrophilic surface on the molecule seems to lead to the subtle change in its interaction with membranes, resulting in the alternation in its biological activity.

Evaluation of Pork Myofibrillar Protein Gel with Pork Skin Gelatin on Rheological Properties at Different Salt Concentrations

  • Lee, Chang Hoon;Chin, Koo Bok
    • Food Science of Animal Resources
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    • v.39 no.4
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    • pp.576-584
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    • 2019
  • This study was performed to evaluate the physicochemical properties of myofibrillar protein (MP) gels containing pork skin gelatin at different salt concentrations. MP gels were prepared to the different salt levels (0.15, 0.30, and 0.45 M) with or without 1.0% of pork skin gelatin. Cooking yield (CY), gel strength, shear stress were measured to determine the physical properties, and SDS-polyacrylamide gel electrophoresis, scanning electron microscopy, fourier transform infrared spectroscopy, sulfhydryl group and protein surface hydrophobicity was performed to figure out the structural changes among the proteins. The addition of gelatin into MP increased CYs and shear stress. MP at 0.45 M salt level had the highest CY and shear stress, as compared to MPs at lower salt concentrations. As the salt concentration of MP gels increased, the microstructure became the compact and wet structures, and decreased the amount of ${\alpha}-helix$/unordered structures and ${\beta}-sheet$. MP with gelatin showed a decreased amount of ${\alpha}-helix$/unordered structures and ${\beta}-sheet$ compared to MP without gelatin. The addition of gelatin to MP did not affect the sulfhydryl group, but the sulfhydryl group decreased as increased salt levels. MP mixtures containing gelatin showed a higher hydrophobicity value than those without gelatin, regardless of salt concentration. Based on these results, the addition of gelatin increased viscosity of raw meat batter and CY of MP gels for the application to low salt meat products.

Effects of Supplementary Vitamin C and E to Moist Pellet Diet on Growth and Body Composition of Juvenile Olive Flounder, Paralichthys olivaceus (습사료에 비타민 C와 E 첨가가 넙치 치어의 성장과 체성분에 미치는 영향)

  • JEONG Gwan Sik;JI Seung Cheol;AHN Chang Bum;SHIN Tae Sun;YOO Jin Hyong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.2
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    • pp.100-106
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    • 2003
  • This study was conducted to investigate the supplementary effects of vitamin C and E to moist pellet on growth and body composition in juvenile olive flounder (Paralichthys olivaceus). Four groups of diet made : moist pellet (MP) diet group was used as the control group where the ratio of raw feed and commercial compound meal is 5:5 $(CP\;30\%,\;CL\;17\%),$ vitamin C added group (VC), vitamin I added group (VE) and vitamin C and I added group (VCE). The supplementary amount of vitamin C and E corresponded to 1000 mg/kg (dry wt.) and 220mg/kg (dry wt.), respectively. Vitamin C was destroyed $50\%$ and vitamin E was destroyed $20\%$ for manufacturing process. After 8 weeks feeding trial, weight gain was $121.9\%$ in MP group, while it ranged from 180.5 to $184.9\%$ in the VC, VE and VCE group. Feed efficiency was $71.\6%$ in MP group, whereas it ranged from 78.7 to $80.6\%$ in the VC, VE and VCE group, weight gain and feed efficiency of fish fed vitamin supplemented diets were significantly higher than control group and no significant difference among the vitamin added groups was observed (p>0.05). There were no significant differences in moisture, crude protein, crude lipid, crude ash, condition factor (CF), hepatosomatic index (HSI), and visceraweight index (VWI) of body composition. Hemoglobin (Hb) was $3.79\%$ in MP group, whereas $4.99\%$ in VC group, $4.55\%$ in VE group and $5.07\%$ in VCE group; Hb was significantly higher in the vitamin added groups than the control group (p<0.05). Vitamin C retention in liver was 18.1, 20.7, 46.6, 54.0 mg/kg in MP, VE, VC and VCE groups, respectively. Vitamin E retention in liver was 25.9, 53.8, 88.2, 124.5 mg/kg in MP, VC, VE and VCE groups, respectively. These results indicate that supplementation of vitamin C and E to moist pellet diet significantly improved growth and feed efficiency of olive flounder, and the mixed addition of vitamin C (1,000 mg/kg dry wt.) and vitamin E (220 mg/kg dry wt.) did not lead to the synergy effect.

Design of AOD System for MP3 Copyright Protection (MP3 저작권 보호를 위한 AOD 시스템의 설계)

  • Kim, Yeong-Jun;Kim, Tae-Yun
    • The KIPS Transactions:PartD
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    • v.9D no.2
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    • pp.323-328
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    • 2002
  • In recent years, e-Commerce is very active on the Internet, especially the World Wide Web alone: with the popularization of Internet using high-speed networks. Especially, Circulation of Multimedia Contents like MP3 data if widely being focused as one of the popular researches. However, the existing models of AOD (Audio On Demand) System lack substantial illegal copy protection or copyright protection. In this paper, we propose an AOD System that guarantees substantial illegal copy protection and copyright protection based on the PKI (Public Key Infrastructure). As transmitting MP3 data using the user's public key, the proposed .method prevents the attack of dropper during transmitting data. Also, it guarantees the right of users and distributors by prohibiting illegal users from using MP3 data.

On-line Delivery Scheme for MP3 Media Data (MP3 미디어 데이터의 온라인 유통 기술)

  • 강상승;임신영;박효준;함호상;김태윤
    • Proceedings of the CALSEC Conference
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    • 1999.11a
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    • pp.589-600
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    • 1999
  • 인터넷 전자상거래 시장의 성장에 따라 디지털 상품에 대한 온라인 판매가 가능하게 되었다. 그러나 현재의 디지털 상품 온라인 유통 체계는 저작권 및 사용자 사용권을 제대로 보장해 주지 못하고 있다. 디지털 상품 중에서도 가장 큰 이슈인 MP3 미디어 데이터는 불법 재생 및 복제에 대해 무방비 상태에 놓여 있다고 해도 과언이 아니다. 본 논문에서는 동적 사용권 관리 기술을 활용한 효율적인 MP3 미디어 데이터 온라인 유통 시스템을 제안한다. 제안한 시스템은 사용권 보호를 원하는 사용자와 저작권 및 불법 재생과 배포의 차단을 원하는 저작사 및 지적 재산권 소유자의 요구 조건을 충족시켜 준다. 추가적으로 MP3 미디어 데이터의 불법 재생 및 배포를 차단할 수 있는 MP3 미디어 데이터 유통 시스템의 궁극적인 모델을 제시한다.

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Efficient Scientific Computation on WP Parallel Computer (MP 병렬컴퓨터에서 효과적인 과학계산의 수행)

  • 김선경
    • Journal of Korea Society of Industrial Information Systems
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    • v.8 no.4
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    • pp.26-30
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    • 2003
  • The Lanczos algorithm is the most commonly used in approximating a small number of extreme eigenvalues for symmetric large sparse matrices. Global communications in MP(Message Passing) parallel computer decrease the computation speed. In this paper, we introduce the s-step Lanczos method, and s-step method generates reduction matrices which are similar to reduction matrices generated by the standard Lanczos method. One iteration of the s-step Lanczos algorithm corresponds to s iterations of the standard Lanczos algorithm. The s-step method has the minimized global communication and has the superior parallel properties to the standard method. These algorithms are implemented on Cray T3E and performance results are presented.

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Quality Assessment and Comparison of Several Radioimmunoassay Kits and Chemiluminescence Immunoassay Methods for Evaluating Serum Estradiol (혈중 Estradiol 농도 측정을 위한 여러 방사면역측정 검사키트 및 화학면역발광 검사법의 성능평가 및 상호비교)

  • Choi, Sung Hee;Noh, Gyeong Woon;Kim, Jin Eui;Song, Yoo Sung;Paeng, Jin Chul;Kang, Keon Wook;Lee, Dong Soo
    • The Korean Journal of Nuclear Medicine Technology
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    • v.19 no.1
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    • pp.72-80
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    • 2015
  • Purpose Serum estradiol ($E_2$) measurement is requested for diagnosing menstrual cycles, ovulation induction, infertility, and menopause. $E_2$ is measured using several methods and kits including radioimmunoassay (RIA) and chemiluminescece immunoassay (CLIA). The purpose of this study was to evaluate quality of these methods and to compare them with each other. Materials and Methods Seven radioimmunoassay kits and two CLIA methods were included in the analysis. Using standard samples and patient samples, intra-assay precision, inter-assay precision, correlation between other methods, sensitivity, and recovery rate were evaluated. Results For all tested kits and methods, coefficients of variance (CVs) of intra-assay precision test were 10.9~13.6% in low-level samples and less than 10% in medium and high-level samples. CVs of inter-assay precision test were 10.8~12.3% in low-level samples and less than 10% in medium and high-level samples with all tested kits and methods. Recovery rates were $92.7{\pm}12.4%$ for SIEMENS, $101.4{\pm}18.4%$ for DIAsource, $95.1{\pm}11.5%$ for AMP, $108.4{\pm}18.5%$ for BECKMAN COULTER, $104.2{\pm}13.5%$ for BECKMAN COULTER Ultra Sensitive, $101.3{\pm}11.6%$ for CIS Bio, and $93.1{\pm}13.2%$ for MP kits. Sensitivity was 7.5, 6.2, 5.7, 6.2, 5.3, 4.5, and 5.5 pg/mL for SIEMENS, DIAsource, AMP, BECKMAN COULTER, BECKMAN COULTER Ultra Sensitive, CIS Bio, and MP kits, respectively. The measurement by MP kit was slightly higher than those by other kits in low-level samples, and the measurement by E170 was slightly higher than those of other kits in medium and high-level samples. In the measurement of standard sample for external quality control, SIEMENS kit produced relatively lower values whereas E170, Architect, and MP kits produced relatively higher values compared with other kits. Conclusion All tested kits for $E_2$ measurement have satisfactory performance for clinical use. However, correlation between kits should be considered when test kits are to be changed, because some pairs of kits do not have correlations with each other.

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Effects of Protein Functionality on Myofibril Protein-Saccharide Graft Reaction

  • Kim, Tae-Kyung;Yong, Hae In;Cha, Ji Yoon;Kim, Yun Jeong;Jung, Samooel;Choi, Yun-Sang
    • Food Science of Animal Resources
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    • v.42 no.5
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    • pp.849-860
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    • 2022
  • The myofibril protein (MP) isolate-saccharide graft reactions was prepared using the Maillard reaction with saccharides. The effects of various saccharides on protein functionality and quality of the Maillard reaction were investigated and compared with those of MP. The grafting degree of the MP isolate-saccharide graft reaction was significantly higher in the reducing sugar-treated groups (lactose, glucose, fructose, and palatinose). The browning intensity of the MP isolate-saccharide graft reaction with fructose, sucrose, and erythitol was higher than that observed in the control reaction (p<0.05). MP that reacted with reducing sugars (glucose, fructose, palatinose, and lactose) had fainter bands than MP that reacted with non-reducing sugars (sucrose, erythitol, trehalose, sorbitol, and xylitol). MPs conjugated with glucose exhibited higher protein solubility. The palatinose and lactose treatments were maximum in water binding capacity, though no significant difference in oil binding capacity among the saccharide treatments was observed. The emulsion stability of the MP isolate-saccharide graft reaction with palatinose and erythitol was higher than that of the control reaction. Therefore, reducing sugars have good protein functionality in the MP isolate-saccharides graft reaction.

Evaluation of Gelation Properties of Salt-Soluble Proteins Extracted from Protaetia brevitarsis Larvae and Tenebrio molitor Larvae and Application to Pork Myofibrillar Protein Gel System

  • Ji Seon Choi;Geon Ho Kim;Ha Eun Kim;Min Jae Kim;Koo Bok Chin
    • Food Science of Animal Resources
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    • v.43 no.6
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    • pp.1031-1043
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    • 2023
  • The purpose of this study was to investigate the functional properties of salt-soluble proteins obtained from Protaetia brevitarsis (PB) and Tenebrio molitor (TM) larvae, the interaction between these proteins and pork myofibrillar protein (MP) in a gel system. The gel properties of salt-soluble protein extracts showed that the PB had a higher viscosity than the TM protein. However, the TM protein had higher gel strength compared with the PB protein. The gelation characteristics of the pork MP gel systems added with lyophilized insect salt-soluble protein powder showed to decrease slightly viscosity compared with MP alone. Adding the TM or PB protein powder did not affect the pork MP's hydrophobicity and sulfhydryl group levels. Furthermore, the protein bands of the MP did not change with the type or amount of insect salt-soluble protein. The cooking yields of the pork MP gels containing PB or TM protein powder were higher than those without insect protein. Regardless of the type of insect salt-soluble protein added, the pork MP's gel strength decreased. Furthermore, as the level of insect powder increased, the surface protein structure became rough and porous. The results demonstrated that proteins extracted from PB and TM larvae interfered with the gelation of pork MP in a gel system.

A Study to Promote the EC Collaboration of the South and North Korea based on the Preemptive Dispute Resolution Policies (남북한 전자상거래 추진에 따른 활성화 방안 -분쟁방지를 위한 사전조정 기능을 중심으로-)

  • Choi, Yong-Rok
    • Journal of Arbitration Studies
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    • v.16 no.3
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    • pp.191-216
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    • 2006
  • It has been talked about the responsibility of the South-North Korean e-market place(e-MP) collaboration project every you since 2003. However, the feasibility as the business has not been dealt with in detail due to the uncertain environment as well as the complicated procedures of the project. The research focuses on the workable mechanism of the South-North Korean E-Market Place collaboration project. The feasibility of the project results from the newly installed fiber telecommunication network as well as the 1 billion dollars of the trading volume between South and North Korea. The research analyzes on the capabilities as well as willingness of the South and North Korea for the e-MP, and concludes with two paradigms of the future tasks. First, the unified but step-wise structure should be made to promote the project by the political committee (South and North Korea EC Committee) and the economical administration body (South and North Korea EC Corporation). Second, the market-oriented resolution system such as South and North Korea Arbitration Body should be involved to create legal environment. Above all, the discussion should be on the main body of e-MP settlement to abolish the psychological as well as practical barriers to enter the South-North Korean electronic commerce.

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