• The Plant Pathology Journal
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• 제37권1호
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• pp.47-56
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• 2021

Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.

• The Plant Pathology Journal
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• 제23권4호
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• pp.281-286
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• 2007

Interactions between viral proteins and host proteins are essential for virus replication. Especially, translation of viral genes completely depends on the host machinery. In potyviruses, interactions of genome-linked viral protein (VPg) with host translation factors including eIF4E, eIF(iso)4E, and poly(A)-binding protein (PABP) has previously been characterized. In this study, we investigated interactions between Soybean mosaic virus (SMV) viral proteins and host translation factors by yeast two-hybrid system. SMV VPg interacted with eIF4E, eIF(iso)4E, and PABP in yeast two-hybrid system, while SMV helper component proteinase (HC-pro) interacted with neither of those proteins. The interaction between SMV NIb and PABP was also detected. These results are consistent with those reported previously in other potyviruses. Interestingly, we found reproducible and specific interactions between SMV coat protein (CP) and PABP. Deletion analysis showed that the region of CP comprising amino acids 116 to 206 and the region of PABP comprising amino acids 520 to 580 are involved in CP/PABP interactions. Soybean library screening with SMV NIb by yeast two-hybrid assay also identified several soybean proteins including chlorophyll a/b binding preprotein, photo-system I-N subunit, ribulose 1,5-biphosphate carboxylase, ST-LSI protein, translation initiation factor 1, TIR-NBS type R protein, RNA binding protein, ubiquitin, and LRR protein kinase. Altogether, these results suggest that potyviral replicase may comprise a multi-protein complex with PABP, CP, and other host factors.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회/대한산업공학회 2003년도 춘계공동학술대회
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• pp.208-216
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• 2003

ISO 14649 (data model for STEP-NC) is a new interface scheme or language for CAD-CAM-CNC chain under established by ISO TC184 SCI. Up to this point, the new language is mainly made for milling and turning, and other processes such as EDM will be completed in the future. Upon completion, it will be used as the international standard language for e-manufacturing paradigm by replacing the old machine-level language, so called M&G code used since 1950's. With the rich information contents included in the new language, various intelligent functions can be made by the CNC as the CNC knows what-to-make and how-to-make. In particular, On-Machine Inspection required for quality assurance in the machine level, can be done based on the information of feature­based tolerance graph. Previously, On-Machine inspection has been investigated mainly for milling operation, and only a few researches were made for turning operation without addressing the data model. In this thesis, we present a feature-based on-machine inspection process by the 4 Tasks: 1) proposing a new schema for STEP-NC data model, 2) converting the conventional tolerance scheme into that of STEP-NC, 3) modifying the tolerance graph such that the tolerance can be effectively measured by the touch probe on the machine, and 4) generating collision-free tool path for actual measurement. Task 1 is required for the incorporation of the presented method in the ISO 14649, whose current version does not much include the detailed schema for tolerance. Based on the presented schema, the tolerance represented in the conventional drafting can be changed to that of STEP-NC (Task 2). A special emphasis was given to Task 3 to make the represented tolerance accurately measurable by the touch probe on the machine even if the part setup is changed. Finally, Task 4 is converting the result of Task into the motion of touch probe. The developed schema and algorithms were illustrated by several examples including that of ISO 14649 Part 12.

• International Journal of Internet, Broadcasting and Communication
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• 제14권4호
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• pp.132-139
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• 2022

The development of the fourth industrial revolution and logistics 4.0 related technology, the growth of the e-commerce market, and the transition to a non-face to face society due to the pandemic are accelerating the growth of the logistics industry. Due to the growth of the logistics industry, various services are emerging to meet the requirements of the market, and research and technology development related to the parcel storage, which is an important element of the last mile service, is also underway. In the past, if it was difficult to deliver the goods directly to the recipient, the parcel storage installed near the delivery location was used, but the usability was not good and the storage of the goods was limited. In addition, the existing parcel storage has a lot of functional limitations compared to the advanced logistics technology, so it is necessary to develop a device that improves it. Therefore, this study conducted to secure safety for unmanned parcel storage devices with robot technology to improve usability and functionality in line with the advanced logistics industry. Based on ISO 10218, an industrial robot related standard, risk identification studies were conducted to derive results that contribute to the development of devices under development.

• 한국전자거래학회지
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• 제4권1호
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• pp.87-101
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• 1999

To operate the automatic devices of manufacturing process more effectively and to solve the needs of the resource sharing, network technology is applied to the control devices located in common manufacturing zone and operated by connecting them. In this paper, functional standard of the network layers are set as physical and data link layer of IEEE 802.2, 802.4, and VMD application layer and ISO-CIM reference model. Then, they are divided as minimized architecture, designed as group objects which perform group management and service objects which organizes and operates the group. For the stability in this network, this paper measures the variation of data packet length and node number and analyzes the variated value of the waiting time for the network operation. For the method of the analysis, non-exhausted service method are selected, and the arrival rates of the each data packet to the nodes that are assumed to form a Poission distribution. Then, queue model is set as M/G/1, and the analysis equation for waiting time is found. For the evalution of the performance, the length of the data packet varies from 10 bytes to 100 bytes in the operation of the group management network, the variation of the wating time is less than 10 msec. Since the waiting time in this case is less than 10 msec, response time is fast enough. Furthermore, to evaluate the real time processing of the group management network, it shows if the number of nodes is less than 40, and the average arrival time is less than 40 packet/sec, it can perform stable operation even taking the overhead such as software delay time, indicated packet service, and transmissin safety margin.

• The Plant Pathology Journal
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• 제39권1호
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• pp.28-38
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• 2023

Plant viruses are responsible for worldwide production losses of numerous economically important crops. The most common plant RNA viruses are positivesense single-stranded RNA viruses [(+)ss RNA viruses]. These viruses have small genomes that encode a limited number of proteins. The viruses depend on their host's machinery for the replication of their RNA genome, assembly, movement, and attraction to the vectors for dispersal. Recently researchers have reported that chloroplast proteins are crucial for replicating (+)ss plant RNA viruses. Some chloroplast proteins, including translation initiation factor [eIF(iso)4E] and 75 DEAD-box RNA helicase RH8, help viruses fulfill their infection cycle in plants. In contrast, other chloroplast proteins such as PAP2.1, PSaC, and ATPsyn-α play active roles in plant defense against viruses. This is also consistent with the idea that reactive oxygen species, salicylic acid, jasmonic acid, and abscisic acid are produced in chloroplast. However, knowledge of molecular mechanisms and functions underlying these chloroplast host factors during the virus infection is still scarce and remains largely unknown. Our review briefly summarizes the latest knowledge regarding the possible role of chloroplast in plant virus replication, emphasizing chloroplast-related proteins. We have highlighted current advances regarding chloroplast-related proteins' role in replicating plant (+)ss RNA viruses.

• 한국산학기술학회논문지
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• 제19권5호
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• pp.570-576
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• 2018

철근콘크리트 구조물에서 철근의 이음은 철근을 겹쳐서 결속선으로 결속한 겹침이음과 커플러에 의한 기계적 이음이 일반적이다. 국내 설계기준(콘크리트구조기준)에서는 일반철근(비용접 철근)에 대해 용접 겹침이음을 제한하고 있으나, 해외기준(AWS D1.4)에서는 예열을 통한 일반철근의 용접 겹침이음을 허용하고 있다. 본 연구에서는 국내외 규격 및 설계기준을 조사하고, 건설현장에서 가장 많이 사용되는 SD400 강종의 일반철근을 대상으로 용접 겹침이음 인장실험을 수행하여 인장강도를 평가하였다. 용접 겹침이음 시험체의 용접길이는 KS규격(KS B ISO 17660-1)에 제시된 최소용접길이인 8d를 적용하고, 예열온도는 AWS D1.4에 따라 D19이하는 $150^{\circ}C$, D22이상은 $260^{\circ}C$로 설정하였다. 인장실험 결과, 용접 겹침이음된 일반철근은 콘크리트구조기준에서 요구하는 인장강도(항복강도의 125%)를 발현하였으며, 모두 철근 모재에서 파단이 발생되었다. 예열 유무에 따른 영향을 검토하기 위해, 일반철근을 예열한 후 용접한 것과 예열 등의 열처리하지 않은 것을 비교한 결과, 인장강도는 큰 차이를 보이지 않았다. 실험시 사용한 일반철근을 대상으로 화학성분을 분석하여 탄소당량(Ceq)을 확인한 결과, 0.45% 이하로 낮았다. AWS D1.4에 따르면, 탄소당량이 0.45% 이하면 예열이 필요하지 않은 조건이다. 결과적으로 예열의 영향이 미비한 것은 일반철근의 탄소당량이 낮았기 때문인 것으로 확인되었다.

• 분석과학
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• 제18권4호
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• pp.271-277
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• 2005

혈액 내 urea는 임상진단 시 신장 기능을 판단하는 중요한 표지물질로서 측정되고 있다. 단백질 등 질소화합물의 최종 대사물인 urea는 콩팥의 사구체에서 걸러져 소변으로 배출되는데, 사구체의 거르는 능력이 저하되면 결국 혈액 속의 urea 농도가 증가하게 되어 신장 기능의 정상여부를 판단할 수 있게 된다. 이러한 임상진단 결과의 신뢰성 향상을 위해서는 측정결과가 일차분석법으로 인증된 인증표준 물질과 소급성 고리를 유지해야 한다. 본 연구에서는 혈청 내 urea의 일차분석법으로서 $15^N_2$-urea를 내부 표준물질로 사용하는 동위원소희석 액체크로마토그라피-질량분석법 (ID-HPLC/MS)을 개발하였다. 이 방법은 측정원리상 고도의 정확성이 확보될 뿐 아니라 별도의 유도체화가 필요 없기 때문에 빠르고 편리하다. $C_{18}$-분리관에 0.1 mmol/L $NH_4Cl$ buffer를 이동상으로 사용하여 urea를 분리하였는데, 이 완충용액은 비교적 분자량이 작은 urea를 질량분석하는데 방해가 크지 않은 장점이 있다. HPLC와 질량분석기의 인터페이스로서 positive mode의 electrospray ionization (ESI)를 사용하여 높은 감도와 재현성을 성취하였다. 국제적으로 인정된 인증표준물질의 분석을 통해 최적화된 방법의 유효성을 확인하였으며, 국제비교시험에도 참여하여 좋은 결과를 얻었다. ISO guide에 따라 불확도를 계산하였으며, 확장 불확도는 95% 신뢰도에서 약 1.8%로 나타났다. 이 분석법은 표준연에서 개발 중인 혈청인증표준물질을 인증하는 일차기준측정절차로도 사용되고 있다.

• 한국산업보건학회지
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• 제19권3호
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• pp.213-232
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• 2009

This document was prepared to review and summarize the analytical methods for airborne and bulk asbestos. Basic principles, shortcomings and advantages for asbestos analytical instruments using phase contrast microscopy(PCM), polarized light microscopy(PLM), X-ray diffractometer (XRD), transmission electron microscopy(TEM), scanning electron microscopy(SEM) were reviewed. Both PCM and PLM are principal instrument for airborne and bulk asbestos analysis, respectively. If needed, analytical electron microscopy is employed to confirm asbestos identification. PCM is used originally for workplace airborne asbestos fiber and its application has been expanded to measure airborne fiber. Shortcoming of PCM is that it cannot differentiate true asbestos from non asbestos fiber form and its low resolution limit ($0.2{\sim}0.25{\mu}m$). The measurement of airborne asbestos fiber can be performed by EPA's Asbestos Hazard Emergency Response Act (AHERA) method, World Health Organization (WHO) method, International Standard Organization (ISO) 10312 method, Japan's Environmental Asbestos Monitoring method, and Standard method of Indoor Air Quality of Korea. The measurement of airborne asbestos fiber in workplace can be performed by National Institute for Occupational Safety and Health (NIOSH) 7400 method, NIOSH 7402 method, Occupational Safety and Health Administration (OSHA) ID-160 method, UK's Health and Safety Executive(HSE) Methods for the determination of hazardous substances (MDHS) 39/4 method and Korea Occupational Safety and Health Agency (KOSHA) CODE-A-1-2004 method of Korea. To analyze the bulk asbestos, stereo microscope (SM) and PLM is required by EPA -600/R-93/116 method. Most bulk asbestos can be identified by SM and PLM but one limitation of PLM is that it can not see very thin fiber (i.e., < $0.25{\mu}m$). Bulk asbestos analytical methods, including EPA-600/M4-82-020, EPA-600/R-93/116, OSHA ID-191, Laboratory approval program of New York were reviewed. Also, analytical methods for asbestos in soil, dust, water were briefly discussed. Analytical electron microscope, a transmission electron microscope equipped with selected area electron diffraction (SAED) and energy dispersive X-ray analyser(EDXA), has been known to be better to identify asbestiform than scanning electron microscope(SEM). Though there is no standard SEM procedures, SEM is known to be more suitable to analyze long, thin fiber and more cost-effective. Field emission scanning electron microscope (FE-SEM) imaging protocol was developed to identify asbestos fiber. Although many asbestos analytical methods are available, there is no method that can be applied to all type of samples. In order to detect asbestos with confidence, all advantages and disadvantages of each instrument and method for given sample should be considered.