• Title/Summary/Keyword: eEF1A

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Data Build-up for the Construction of Korean Specific Greenhouse Gas Emission Inventory in Livestock Categories

  • Won, S.G.;Cho, W.S.;Lee, J.E.;Park, K.H.;Ra, C.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.3
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    • pp.439-446
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    • 2014
  • Many studies on methane ($CH_4$) and nitrous oxide ($N_2O$) emissions from livestock industries have revealed that livestock production directly contributes to greenhouse gas (GHG) emissions through enteric fermentation and manure management, which causes negative impacts on animal environment sustainability. In the present study, three essential values for GHG emission were measured; i.e., i) maximum $CH_4$ producing capacity at mesophilic temperature ($37^{\circ}C$) from anaerobically stored manure in livestock category ($B_{0,KM}$, Korean livestock manure for $B_0$), ii) $EF_{3(s)}$ value representing an emission factor for direct $N_2O$ emissions from manure management system S in the country, kg $N_2O-N$ kg $N^{-1}$, at mesophilic ($37^{\circ}C$) and thermophilic ($55^{\circ}C$) temperatures, and iii) $N_{ex(T)}$ emissions showing annual N excretion for livestock category T, kg N $animal^{-1}$ $yr^{-1}$, from different livestock manure. Static incubation with and without aeration was performed to obtain the $N_2O$ and $CH_4$ emissions from each sample, respectively. Chemical compositions of pre- and post- incubated manure were analyzed. Contents of total solids (% TS) and volatile solid (% VS), and the ratio of carbon to nitrogen (C/N) decrease significantly in all the samples by C-containing biogas generation, whereas moisture content (%) and pH increased after incubation. A big difference of total nitrogen content was not observed in pre- and post-incubation during $CH_4$ and $N_2O$ emissions. $CH_4$ emissions (g $CH_4$ kg VS-1) from all the three manures (sows, layers and Korean cattle) were different and high C/N ratio resulted in high $CH_4$ emission. Similarly, $N_2O$ emission was found to be affected by % VS, pH, and temperature. The $B_{0,KM}$ values for sows, layers, and Korean cattle obtained at $37^{\circ}C$ are 0.0579, 0.0006, and 0.0828 $m^3$ $CH_4$ kg $VS^{-1}$, respectively, which are much less than the default values in IPCC guideline (GL) except the value from Korean cattle. For sows and Korean cattle, $N_{ex(T)}$ values of 7.67 and 28.19 kg N $yr^{-1}$, respectively, are 2.5 fold less than those values in IPCC GL as well. However, $N_{ex(T)}$ value of layers 0.63 kg N $yr^{-1}$ is very similar to the default value of 0.6 kg N $yr^{-1}$ in IPCC GLs for National greenhouse gas inventories for countries such as South Korea/Asia. The $EF_{3(s)}$ value obtained at $37^{\circ}C$ and $55^{\circ}C$ were found to be far less than the default value.

Chemical Properties of Indoor Individual Particles Collected at the Daily Behavior Spaces of a Factory Worker

  • Ma, Chang-Jin;Kang, Gong-Unn;Sakai, Takuro
    • Asian Journal of Atmospheric Environment
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    • v.11 no.2
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    • pp.122-130
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    • 2017
  • The main purpose of the study was to clarify the properties of individual particles collected at each behavior space of a factory worker. The samplings of size-segregated ($PM_{2.1-1.1}$ and $PM_{4.7-3.3}$) indoor particles were conducted at three different behavior spaces of a factory worker who is engaged in an auto parts manufacturing plant (i.e., his home, his work place in factory, and his favorite restaurant). Elemental specification (i.e., relative elemental content and distribution in and/or on individual particles) was performed by a micro-PIXE system. Every element detected from the coarse particulate matters of home was classified into three groups, i.e., a group of high net-counts (Na, Al, and Si), a group of intermediate net-counts (Mg, S, Cl, K, and Ca), and a group of minor trace elements (P, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, and Pb). The results of EF for $PM_{4.7-3.3}$ in home indicated that several heavy metals were generated from the sources within the house itself. An exceptional feature shown in the individual particles in workplace is that Cr, Mn, and Co were clearly detected in both fine and coarse particles. Cluster analysis suggested that the individual coarse particles ($PM_{4.7-3.3}$) collected at the indoor of factory were chemically heterogeneous and they modified with sea-salt, mineral, and artificially derived elements. The principal components in individual coarse particles collected at restaurant were sea-salt and mineral without mixing with harmful trace elements like chromium and manganese. Compared to the indoor fine particles of home and restaurant, many elements, especially, Cl, Na, Cr, Mn, Pb, and Zn showed overwhelmingly high net-counts in those of factory.

Analysis of the Effect of Construction of Public Cremation Facilities in Jeongeup City on Cremators Number Using E-Haneul Funeral Information System in Jeollabukdo and on Cremation Rate

  • Choi, Jae-sil;Kim, Jeong-lae
    • International journal of advanced smart convergence
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    • v.10 no.1
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    • pp.117-124
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    • 2021
  • Based on a result of we research and analysis of this study, it was analyzed that the average annual cremators number using E-Haneul Funeral Information System among the five public cremation facilities in Jeollabukdo was 9,713 in total before the opening of public creation facilities in Jeongeup City (2013-2015), while the number was 12,159 after the opening of public cremation facilities in Jeongeup City (2016-2019); the number was increased by 2,446, compared to the opening period (2013-2015), with a large increase rate of 25.2%. In addition, the average annual cremation rate before the opening of public cremation facilities in Jeongeup City (from 2013 to 2015) was 71.6%, but the average annual cremation rate during the period after the opening (from 2016 to 2019) was 81.5%, which was a large increase rate of 9.9% compared to period before the opening of the facilities. Based on research results above, we have presented policy suggestions in order to increase the efficiency of operation and management of public cremation facilities by local governments as follows. First, in order to prepare for the increase in demand for cremation due to the increase in cremators number, a policy promotion for the expansion of "public cremation facilities" should be carried out as soon as possible, focusing on local governments whose supply has reached the limit. Second, in order to support the expansion and construction of public cremation facilities, the government subsidy rate and government subsidy at the level of current 70%, provided by the central government, should be further expanded. Third, overcoming theNIMBY conflict overlocation for public cremation facilities, theutilization rate should be enhanced, through the joint construction and facility operation of the public cremation facilities between local governments, and the ef iciency of the project implementation should be improved through the jointsharing of the facility operation cost.

A Study on the Emission Characteristics and Prediction of VOCs (Volatile Organic Compounds) using Small Chamber Method (소형챔버법을 이용한 휘발성유기화합물(VOCs) 방출특성 및 예측에 관한 연구)

  • Pang, Seung-Ki;Sohn, Jang-Yeul;Lee, Kwang-Ho
    • KIEAE Journal
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    • v.4 no.4
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    • pp.11-18
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    • 2004
  • In this study, the measurement system was developed for the measurement of pollutants from building materials, and specimens were made with concrete, gypsum board, mortar and wall paper. Characteristics of VOCs and TVOC concentration and Emission Factor as a function of time were assessed, and the conclusion was drawn as follows. (1) From predicting TVOC concentration decrease of specimen 7 with the wall paper attached to the concrete, the graph may become linear by converting the value of y-axis into the log function, and the prediction equation can be expressed as $y=34906{\ast}e^{-0.0093{\ast}time}$. Moreover, chi-square value was 0.83 which is relatively high value, indicating that TVOC concentration can be properly predicted if the same materials are used indoors. (2) From predicting VOCs Emission Factor decrease of specimen 7, the prediction equation can be expressed as $EF=15111{\ast}e^{-0.0093{\ast}time}$, and chi-square value was 0.83. (3) From predicting TVOC concentration decrease of specimen 7, prediction equation can be considered to be $y=254323{\ast}(1-e^{-0.1046{\ast}time})$, and chi-square was 0.994 which is significantly high value, indicating that indoor TVOC concentration can be properly predicted if the same materials are used indoors. Furthermore, the prediction of concentration decrease using cumulative value of hourly measured concentration is considered to be more accurate than that using just hourly measured value directly. (4) From predicting Emission Factor decrease with cumulative hourly data of Emission Factor, chi-square appeared to be higher than that by just using hourly data of Emission Factor directly. Therefore, the prediction of Emission Factor with cumulative hourly data can provide more reliable prediction equation than the case by using just hourly concentration directly.

Impact of Hemodialysis on Left Ventricular Performance: A Doppler Echocardiographic Study (혈액투석 유지요법 환자에서 투석 전후의 좌심실 Doppler 심초음파를 이용한 기능지표의 변화)

  • Kang, Dong-Oh;Lee, Du-Ha;Kim, Hyun-Seo;Kim, Hyun-Su;Kim, Sung-Rok;Park, Jong-Seon
    • Journal of Yeungnam Medical Science
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    • v.16 no.2
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    • pp.309-317
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    • 1999
  • Background: Left ventricular diastolic filling is an important determinant for maintenance of cardiac output during hemodialysis. Few investigators have studied the influence of hemodialysis on diastolic function. To evaluate the change of left ventricular systolic and diastolic function. we performed M-mode and Doppler echocardiographic studies before and after hemodialysis. Methods: The study population consisted of 30 patients(15 patients were male, mean age $45{\pm}10$ years) with CRF on maintenance hemodialysis. They have normal left ventricular systolic function(Fractional shortening>30%) and no evidence of valvular heart disease or regional wall motion abnormalities. The ejection fraction (EF) was measured using M-mode echocardiography and Doppler indices such as peak E velocity, peak A velocity, isovolumetric relaxation time(IVRT), deceleration time(DT). and left ventricular ejection time(LVET) obtained from Doppler echocardiography. The index of myocardial performance (IMP) was calculated from each of the Doppler velocity indices. Results: The weight reduction after hemodialysis was $2.1{\pm}1.0kg$(p<0.0001), After hemodialysis, there was some decrease in blood pressure(p<0.05), but no significant change in heart rate, EF and fractional shortening, mean VCF, peak A velocity, and DT. And significant reduction in peak E velocity, E/A ratio(p<0.0001. p<0.001), and significant increase in IVRT and IMP(p<0.05, p<0.0001) were noted. Conclusion: In conclusion, preload reduction is the main mechanism that accounts for changes in Doppler diastolic indices after hemodialysis. And an increased IMP suggests that diastolic function may be aggravated after hemodialysis, and that implies impaired left ventricular filling and disturbed left ventricular compliance.

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Comparison of Expression Pattern of Housekeeping Genes in Mice fed Genetically Modified Rice (유전자 이입에 따른 GM쌀 섭취 마우스의 Housekeeping Gene 발현 패턴 비교)

  • Lee, Dong-Yeob;Heo, Jin-Chul;Lee, Kyu-Hyun;Kim, Dong-Ho;U, Sang-Uk;Cho, Hyun-Suk;Lee, Sang-Han
    • Food Science and Preservation
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    • v.14 no.6
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    • pp.688-694
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    • 2007
  • To evaluate the human risk of long-term intake of genetically modified (GM) rice, we carried out RT-PCR of housekeeping genes. Housekeeping genes, which show highly uniform expression in living organisms during various stages of development and under different environmental conditions, were normalized by RT-PCR. We assessed the expression of 10 common housekeeping genes (18s rRNA, 25S rRNA, UBC, UBQ5, UBQ10, ACT11, GAPDH, eEF-$1{\alpha}$, ${\beta}$-TUB, GAPDH, ${\beta}$-actin, B2m, G6pd2, Gyk, Gus, Hprt, Cyclophlin A, Tfrc, ${\alpha}$-tubulin and RPL13A) in the liver, stomach, small intestine, large intestine, kidney and spleen of mice fed GM or non-GM rice. We found no significant differences in the expression of housekeeping genes between the two groups of mice.

First report of six Sordariomycetes fungi isolated from plant litter in freshwater ecosystems of Korea

  • Goh, Jaeduk;Mun, Hye Yeon;Jeon, Yu-Jeong;Chung, Namil;Park, Young-Hwan;Park, Sangkyu;Hwang, Hyejin;Cheon, Wonsu
    • The Korean Journal of Mycology
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    • v.48 no.2
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    • pp.103-116
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    • 2020
  • Freshwater ecosystems provide a complex environment for microorganisms. In this study, we isolated diverse fungal strains from plant litter in freshwaters. These strains were identified using molecular phylogenetic analyses of rDNA and/or other gene sequences (TUB, GAPDH, and EF1). In addition, we examined their morphological characteristics by microscopy and cultural characteristics on several media. We identified six previously unrecorded Sordariomycetes species in Korea, i.e., Colletotrichum godetiae, Discosia rubi, Robillarda sessilis, Monochaetia dimorphospora, Idriella lunata, and Phialemoniopsis endophytica. Of these, D. rubi and M. dimorphospora exhibited high extracellular amylase, lipase, and protease activities, suggesting that these fungal isolates might play an important role as decomposers in freshwater ecosystems. Plant litter could thus be a good source for isolating and investigating previously undocumented fungal species in freshwater environments.

A Study on the Effect of Virtual Reality Intervention on Cognitive Function in Individuals With Stroke Through Meta-analysis (메타분석을 통한 뇌졸중 환자의 인지기능에 대한 가상현실 중재 효과 연구)

  • Kwon, Jae Sung
    • Therapeutic Science for Rehabilitation
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    • v.10 no.3
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    • pp.7-22
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    • 2021
  • Objective : The purpose of this study was to verify the effect of virtual reality interventions (VRIs) on cognitive function in individuals with stroke through a systematic literature review and meta-analysis. Methods : We reviewed randomized controlled trials (RCTs) the last 10 years using academic databases. PubMed, MEDLINE, and CINAHL were used for international studies, and DBpia, KISS, Kyoboscholar, and e-article were used for Korean studies. For the quantitative meta-analysis, subgroups of outcomes were classified into general cognitive function (G-CF), attention and memory (A&M), and executive function (EF). Results : Nine RCTs were analyzed. The total number of participants was 271 (140 in the experimental group). The effect size (Cohen's d) was estimated using a random effects model. The effect sizes of the outcome subgroups of were as follows: small to medium for G-CF (d=0.422; 95% CI: 0.101~0.742; p=0.010), small for A&M (d=0.249; 95% CI: -0.107~0.605; p=0.170), and medium for EF (d=0.666; 95% CI: 0.136~1.195; p=0.014). Conclusion : Considering the various stimuli provided by the virtual environment and the results from available research, virtual reality should be applied to interventions for integrated cognitive functions. In addition, it would be appropriate to be used as an additional intervention to traditional cognitive rehabilitation for stroke.

A Study on Thermodynamic Properties of Ethylene Gas Hydrate

  • Lim, Gye-Gyu
    • Journal of Korean Society for Atmospheric Environment
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    • v.23 no.E1
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    • pp.10-15
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    • 2007
  • The gas hydrates are probably most sensitive to climate change since they are stable only under specific conditions of high pressure and low temperature. One of the main factors responsible for formation of gas hydrates is the saturation of the gases with water vapor. Quantitative phase equilibrium data and understanding of the roles of water component in the phase behavior of the heterogeneous water-hydrocarbon-hydrate mixture are of importance and of engineering value. In this study, the water content of ethylene gas in equilibrium with hydrate and water phases were analyzed by theoretical and experimental methods at temperatures between 274.15 up to 291.75 K and pressures between 593.99 to 8,443.18 kPa. The experimental and theoretical enhancement factors (EF) for the water content of ethylene gas and the fugacity coefficients of water and ethylene in gas phase were determined and compared with each other over the entire range of pressure carried out in this experiment. In order to get the theoretical enhancement factors, the modified Redlich-Kwong equation of state was used. The Peng-Robinson equations and modified Redlich-Kwong equations of state were used to get the fugacity coefficients for ethylene and water in the gas phase. The results predicted by both equations agree very well with the experimental values for the fugacity coefficients of the compressed ethylene gas containing small amount of water, whereas, those of water vapor do not in the ethylene rich gas at high temperature for hydrate formation locus.

Expression Profiling of WSSV ORF 199 and Shrimp Ubiquitin Conjugating Enzyme in WSSV Infected Penaeus monodon

  • Jeena, K.;Prasad, K. Pani;Pathan, Mujahid Khan;Babu, P. Gireesh
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.8
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    • pp.1184-1189
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    • 2012
  • White spot syndrome virus (WSSV) is one of the major viral pathogens affecting shrimp aquaculture. Four proteins, WSSV199, WSSV 222, WSSV 249 and WSSV 403, from WSSV are predicted to encode a RING-H2 domain, which in presence of ubiquitin conjugating enzyme (E2) in shrimp can function as viral E3 ligase and modulate the host ubiquitin proteasome pathway. Modulation of host ubiquitin proteasome pathway by viral proteins is implicated in viral pathogenesis. In the present study, a time course expression profile analysis of WSSV Open Reading Frame (ORF) 199 and Penaeus monodon ubiquitin conjugating enzyme (PmUbc) was carried out at 0, 3, 6, 12, 24, 48 and 72 h post WSSV challenge by semi-quantitative RT-PCR as well as Real Time PCR. EF1${\alpha}$ was used as reference control to normalize the expression levels. A significant increase in PmUbc expression at 24 h post infection (h.p.i) was observed followed by a decline till 72 h.p.i. Expression of WSSV199 was observed at 24 h.p.i in WSSV infected P. monodon. Since the up-regulation of PmUbc was observed at 24 h.p.i where WSSV199 expression was detected, it can be speculated that these proteins might interact with host ubiquitination pathway for viral pathogenesis. However, further studies need to be carried out to unfold the molecular mechanism of interaction between host and virus to devise efficient control strategies for this chaos in the shrimp culture industry.