• Korean Journal Plant Pathology
• /
• v.11 no.1
• /
• pp.73-79
• /
• 1995

Complementary DNAs (cDNAs) to the coat protein gene of an ordinary Korean strain of potato virus Y (PVY-OK) isolated from potato (cv. Superior) were synthesized and cloned into a plasmid pUC119 and sequenced. The RNA of the virus propagated in tobacco (Nicotinaa sylvestris) was extracted by the method of phenol extraction. The first strand of cDNAs to the coat protein penomic RNA of the virus was made by Moloney murine leukemia virus reverse transcriptase. The cDNA were synthesized and amplified by the method of polymerase chain reaction (PCR) using a pair of oligonucleotide primers. PVYCP3P and PVYCP3M. The size of cDNAs inserted in pUC119 plasmid was estimated as about 840 bp upon agarose gel electrophoresis. Double stranded cDNAs were transformed into the competent cell of E. coli JM109. Sequence analysis of cDNAs was conducted by the dideoxynucleotide chain termination method. Homology of cDNAs of the PVY-OK coat protein genomic RNA with those of PVY-O (Japan), PVY-T (Japan), PVY-TH (Japan), PVYN (The Netherlands),and PVYY (France) was represented as 97.3%, 88.9%, 89.3%, 89.6% and 98.5%, respectively. Homology at the amino acid level turned out to the be 97.4%, 92.5%, 92.9%, 92.9% and 98.5%, respectively.

• Journal of Ecology and Environment
• /
• v.37 no.1
• /
• pp.21-29
• /
• 2014

Robinia pseudoacacia has become invasively naturalized in Japan. We investigated the role of sexual reproduction in the development of R. pseudoacacia riparian forests along the Chikumagawa River in Japan, by using five chloroplast (cpSSR) and seven nuclear (nSSR) markers. We identified eight chloroplast haplotypes and 147 nuclear genotypes from 619 R. pseudoacacia trees sampled in three plots (Plots A, B, and C) and along two line transects (Lines D and E). CpSSR analyses showed that multiple maternal lines were distributed along the river, and that some haplotypes from different populations overlapped. In addition, while Plots A and B were separated by a short distance, only these two plots exhibited genetic differentiation in the haplotypes. In the nSSR analysis, all pairwise $F_{ST}$ values among the three plots were significantly different from zero. Kinship analysis based on nSSR markers revealed that kinship connected many individuals to another individual from the same plot. These results indicate that seed dispersal near to mother trees contributes to the fine-scale genetic structure of R. pseudoacacia riparian forests. Our results indicate that sexual reproduction, in addition to asexual reproduction, is a major contributor to the fine-scale formation of R. pseudoacacia forests.

• Carbon letters
• /
• v.5 no.3
• /
• pp.108-112
• /
• 2004

Effect of silicon infiltration on the bend and tensile strength of 2D cross-ply carbon-carbon composites are studied. It is observed that bend strength higher than tensile strength in both types of composite is due to the different mode of fracture and loading direction. After silicon infiltrations bend and tensile strength suddenly decreases of carbon-carbon composites. This is due to the fact that, after silicon infiltration, silicon in the immediate vicinity of carbon forms the strong bond between carbon and silicon by formation silicon carbide and un-reacted silicon as free silicon. Therefore, these composites consist of three components carbon, silicon carbide and silicon. Due to mismatch between these three components secondary cracks developed and these cracks propagate from $90^{\circ}$ oriented plies to $0^{\circ}$ oriented plies by damaging the fibers (i.e., in-situ fiber damages). Hence, secondary cracks and in-situ fiber damages are responsible for degradation of mechanical properties of carbon-carbon composites after silicon infiltration which is revealed by microstructure investigation study by scanning electron microscope.

• Animal Systematics, Evolution and Diversity
• /
• v.35 no.1
• /
• pp.33-36
• /
• 2019

The marine interstitial annelid Pharyngocirrus uchidai(Sasaki, 1981) has been only known from Japan. In this study, we report the occurrence of P. uchidai for the first time in four localities along the eastern coast of Korea: Bukmyeon, Gamchu, Gase, and Oeongchi. Species identification was confirmed by comparison of DNA barcoding sequences with morphological examination from the type locality, Oshoro, Japan. We generated a total of 25 sequences of a partial segment (580 bp) of the cytochrome c oxidase subunit I gene (COI), representing five specimens from each locality. Maximum intra-specific variation was 1.2% in terms of Kimura two-parameter (K2P) distance, observed between two individuals each from Gamchu (i.e., between two specimens from the single locality), Gamchu and Oeongchi, Gamchu and Oshoro, and Oeongchi and Oshoro. On the other hand, an identical haplotype was found in all the five localities, substantiating our species identification for the Korean populations. Inter-specific K2P distance between P. uchidai and an unidentified Saccocirrus sp. from Canada (based on public database entries) was 22.4-23.4%.

• Journal of Conservation Science
• /
• v.35 no.1
• /
• pp.51-60
• /
• 2019

This paper provides quantitative data that describes the evolution of the air quality in the Whale and Sea Museum, located in the Iwate prefecture, collected after the 2011 Great East Japan Earthquake and tsunami. The museum was damaged significantly by the disaster, and restoration works continued for over six years. The air quality in the temporary storage facility and museum was monitored during the rehabilitation process. Evaluation of air quality is carried out by gas chromatography- mass spectrometry, ion chromatography and high-performance liquid chromatography. The results showed that the characteristics of the chemical components differed depending on the measurement locations inside the building. The museum atmosphere tended to be alkaline as the airtightness increased because of the maintenance works at the entrance. It was also determined that it was necessary to study the intake/exhaust routes and to clean them according to the contamination degree. In Japan, there are recommended museum air quality standards for acetic acid, formic acid, alkali, and aldehydes. The results indicated that these standards should not be used as a reference for damaged museums. Furthermore, at the temporary storage facilities for to store the collections during the rehabilitation of the museum, solvents such as ethyl benzene, toluene, and xylene are initially abundant, although they can be reduced by ventilation, while other components such as 2E1H was confirmed in this case are likely to remain.

• The Journal of Industrial Distribution & Business
• /
• v.13 no.11
• /
• pp.47-64
• /
• 2022

Purpose: This study's goal is to investigate how perceived risk of Japanese electronic product affect the negative emotion and the avoidance intentions. In addition, this is difference in the effect of perceived risk on negative emotions and avoidance intentions according to the presence or absence of substitutes. Research design, data and methodology: Perceived risks of Japan products are decided by four dimensions, they are economic risk, social risk, psychological risk and physical risk. The reach model is made by the theory of risk-avoid. We requested this survey to 5808 customers by panel and web site, received 559 replied. We used 528 questionnaires excluding unreliable data. For the analysis, smart PLS is used. Results: Psychological risk has influence on negative emotion and avoidance intension. Social risk and physical risk affected negative emotion, but did not directly affect avoidance intention. Economic risk affects avoidance intension, but it has no effect of negative emotion. The existence of Japanese products' substitute only effects the relationship of economic risk and avoidance intention. Conclusions: Korean consumers behavior their buying and using of Japan product as financial benefit and satisfaction, not only risk. It is suggested that Korean company should make and develop unique product with good price.

• Asia Marketing Journal
• /
• v.17 no.4
• /
• pp.43-61
• /
• 2016

This paper has identified some theoretical reasons and empirical evidence for negative scores that occur in Japan and Korea or unstable NPS scores that can be experienced. A psychological analysis of NPS results sheds light on the validity of the negative NPS scores that are often found in Japan and Korea. Usually customer experience surveys utilize a "single stimulus" such as the "company" or the "company's products / services." However, in the case of the "recommendation to friend" question of the NPS system there are two stimuli namely the "company product/service" and the influence of "friends." Hence, the survey outcomes from this question can be very different when compared with other single stimulus questions such as "overall satisfaction" or "repurchase." Japanese and Korean people may have a positive attitude towards the company but they will provide low NPS scores because they are reflecting that they would not run the risk of ruining their relationships with their friends by making a recommendation. As a result, in the NPS system these people will be labeled as "detractors" when in fact they are "ambivalent customers." Using several Japanese and Korean based marketing research industry examples and case studies, different strategies are proposed to address the issue of negative scores in the NPS system in Japan and Korea. The Customers Psyche appears to be the key determinant factors for both types of behavioural items (items with a single stimulus as well as items with two stimuli).

• International conference on construction engineering and project management
• /
• 2013.01a
• /
• pp.315-320
• /
• 2013

The aim of this study is to compare efficiency and Total Factor Productivity(TFP) between Korean and Japanese construction firms in Korea and Japan over the period of 2005-2011. The results of this study are as follows. Efficiency scores of Korean construction firms are 0.797, and Japanese construction firms are 0.921. Second, annual total factor productivity growth of Korean construction firms is 0.5% and technical progress do much for TFP decrease. However Japanese construction firms marked annual increasing of 2.5% of TFP. Third, technical progress contributed in TFP increase of construction firms in Korea. Korean construction firms, however, relatively lagged behind Japanese construction firms in technical progress. Therefore, Korean construction firms need strategies to achieve technical advances including adopting new technology or process innovation to maintain competitiveness, survive, and develop in the future competition with Japan.

• Resources Recycling
• /
• v.20 no.3
• /
• pp.12-27
• /
• 2011

DOWA group has been working on metal recycling applying the smelting and refining process of KOSAKA Smelter. DOWA has developed it's metal recycling technologies through the treatment of black ore(complex sulfide ores) that contain many kinds of non-ferrous metals. In addition to these special technologies, DOWA has strengthened its hydrometallurgical process of precious metals and ability to deal with low-grade materials such as used electrical appliances or vehicles. On the other hand, JX Nippon Mining & Metals Corporation(JX-NMMC) carries out its metal recycling and industrial waste treatment businesses employing advanced separation, extraction and refining technologies developed through its extensive experience in the smelting of non-ferrous metals. JX-NMMC collects approximately 100,000t/y of copper and precious metal scraps from waste sources such as electronic parts, mobile phones, catalytic converters, print circuit boards and gold plated parts. These items are recycled through the smelting and refining operations of Saganoseki smelter and Hitachi Metal-recycling complex(HMC). In this like, metal recycling industries combined with environmental business service in Japan have been developed through excellent technologies for mineral processing and non-ferrous smelting. Also, both group, Dowa and JX-NMMC, were contributed to establish Japan's recycling-oriented society as the typical leading company of non-ferrous smelting. Now. it is an important issue to set up the collection system for e-waste.

• Korean Journal of Food Science and Technology
• /
• v.48 no.5
• /
• pp.430-436
• /
• 2016

Solid phase microextraction and gas chromatography-mass spectrometry (SPME/GC-MS), electronic nose, and electronic tongue were used to characterize the sensory profiles of cider vinegars from Korea (K1-2), China (C1-2), Japan (J1-2), and US (U1-2). SPME-GC/MS detected acetic acid as the common volatile compound in all vinegars, in addition to isovaleric acid, octanoic acid, and phenethyl acetate. Acids and acetic esters were the major components of Korean and US vinegar samples, respectively. Chinese vinegars had high ethyl acetate content, while Japanese samples were characterized by a low content of acetic acid. Principal component analysis (PCA) pattern provided a clear categorical discrimination of Chinese vinegars by E-nose and E-tongue analyses. The instrumental sensory scores and the taste attributes for flavor ($r^2=0.9431$), sourness ($r^2=0.9515$), and sweetness ($r^2=0.8325$) were highly correlated. Therefore, SPME/GC-MS, E-nose, and E-tongue analyses may be useful tools to discriminate the sensory profiles of cider vinegars of different origins.