• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.12
• /
• pp.1689-1695
• /
• 2008

Leptin, the hormone product of the obese gene, is secreted predominately from white adipose tissue and regulates feed intake, energy metabolism and body composition. It has been considered a candidate gene for performance, carcass and meat quality traits in beef cattle. The objective of this study was to identify SNPs in the promoter region of the leptin gene and to evaluate the possible association of the SNP genotypes with carcass and meat quality traits in Korean cattle. We identified a total of 25 SNPs in the promoter region (1,208-3,049 bp upstream from the transcription start site) of the leptin gene, eleven (g.1508C>G, g.1540G>A, g.1545G>A, g.1551C>T, g.1746T>G, g.1798ins(G), g.1932del(T), g.1933del(T), g.1934del(T), g.1993C>T and g.2033C>T) of which have not been reported previously. Their sequences were deposited in GenBank database with accession number DQ202319. Genotyping of the SNPs located at positions g.2418C>G and g.2423G>A within the promoter region was performed by direct sequencing and PCR-SSCP method to investigate the effects of SNP genotypes on carcass and meat quality traits in Korean cattle. The SNP and SSCP genotypes from the two mutations of the leptin promoter were shown to be associated with the BF trait. The average BF value of animals with heterozygous SNP genotype was significantly greater than that of animals with the homozygous SNP genotypes for the g.2418C>G and g.2423G>A SNPs (p<0.05). Analysis of the combined genotype effect in both SNPs showed that animals with the AC SSCP genotype had higher BF value than animals with BB or AA SSCP genotypes (p<0.05). These results suggest that SNP of the leptin promoter region may be useful markers for selection of economic traits in Korean cattle.

• The Korean Journal of Quaternary Research
• /
• v.17 no.2
• /
• pp.145-148
• /
• 2003

The Kunang cave paleolithic site is located at Tanyang [$N37^{\circ}2'$, $128^{\circ}21'E$], Chungbuk Province, which is in the Central part of the Korean peninsula. The cave is developed at 312 amsl in a karstic mountainous area. The South Han River flows across this region and other caves can also be found near the river. The site was discovered in 1986 and excavated 3 times by the Chungbuk National University Museum until now. The cave was wellpreserved from modem human activities until the first discovery. The full length of the cave is estimated to be ca. 140 m. However, a spacious part up to 11 m from the entrance has been excavated. Eight lithological units are divided over the vertical profile at a depth of 5 m. Each unit is deposited in ascending order as follow: mud layer (Unit 9), lower complex (Unit 8) which is composed of angular blocks and fragments with a muddy matrix, lower travertine layer (Unit 7； flowstone), middle complex (Unit 6; cultural layer) which is composed of fragments with a muddy matrix, middle travertine layer (Unit 5； flowstone), yellowish muddy layer (Unit 4), upper complex (Unit 3； cultural layer) which has a similar composition to Unit 8. the upper travertine layer (Unit 2； flowstone), and finally surface soil layer (Unit 1). The most abundant vestiges in the cultural layers are the animal bones. They are small fractured pieces and mostly less than 3 cm in length. About 3,800 bone pieces from 25 animal species have been collected so far, 90 percent of them belonging to young deers. Previous archaeological study of these bone pieces shows thatprehistoric people occupied the cavenot for permanent dwelling but for temporary shelter during their seasonal hunting activity. More extensive studies of these bones together with pollen analysis are in progress to reconstruct the paleoenvironment of this cave. Only a single date (12,500 BP) obtained from a U-Th measurement of the upper travertine layer was previously available. In spite of the importance of the cave stratigraphy, there was no detail chronological investigation to establish the depositional process of the cultural layers and to understand the periodic structure of the cave strata, alternating travertine floor and complex layers. We have measured five 14C age dating (38900+/-1000, 36400+/-900, 40600+/-1600, more than 51000 and 52000 14C BP) using Seoul National University 14C AMS facility, conducted systematic process of the collagen extraction from bone fragments samples. From the result, we estimate that sedimentation rate of the cave earth is constant, and that the travertine layers, Unit 2 and Unit 3, was formed during MIS 5a(ca. 80 kBP) and MIS 5c (ca. 100 kBP) respectively. The Kunang Cave site is located at Yochonli of the region of Danyang in the mid-eastern part of Korea. This region is compased of limestones in which many caves were found and the Nam-han river flows meanderingly. The excavations were carried out three times in 1986, 1988, and 1998.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.11 no.2
• /
• pp.720-726
• /
• 2010

Haplotype-based research has become increasingly important in the field of personalized medicine since the haplotype reflects a set of SNPs (Single Nucleotide Polymorphisms) that are genetically associated and inherited together. Currently, the most widely used application softwares available for haplotype reconstruction, based on in silico method, include PL-EM, Haplotyper, PHASE and HAP. PL-EM, Haplotyper and PHASE are command-line application running on LINUX or Unix system and HAP is a web-based client-server application. This paper deals with an integrated haplotype reconstruction system that have been developed with PL-EM and Haplotyper selected from the accuracy test with experimentally verified data on public application softwares. This integrated system is a kind of client-sever one with user friendly web interface and can provide end-users with a high quality of haplotype analysis. SNPs genotype data with a length of 5 derived from 5 people and SNPs genotype data with a length of 13 derived from 15 people were used to test the analysis results of Haplotyper and PL-EM respectively. As a result, this system has been confirmed to provide the systematic and easy-to-understand analysis results that consist of two main parts, i.e. individual haplotype information and haplotype pool information. In this respect, the integration system will be utilized as a useful tool for the discovery of disease related genes and the development of personalized drugs through facilitating the reconstruction of haplotype maps.

• Interdisciplinary Bio Central
• /
• v.1 no.1
• /
• pp.1.1-1.5
• /
• 2009

Many organisms control their physiology and behavior in response to the local light environment, which is first perceived by photoreceptors that undergo light-dependent conformational changes. Phytochromes are one of the major photoreceptors in plants, controlling wide aspects of plant physiology by recognizing the light in red (R) and far-red (FR) spectra. Higher plants have two types of phytochromes; the photo-labile type I (phyA in Arabidopsis) and photo-stable type II (phyB-E in Arabidopsis). Phytochrome B (phyB), a member of the type II phytochromes in Arabidopsis, shows classical R and FR reversibility between the inter-convertible photoisomers, Pr and Pfr. Interestingly, the Pr and Pfr isomers show partitioning in the cytosol and nucleus, respectively. In the over 50 years since its discovery, it has been thought that the type II phytochromes only function to mediate R light. As described in the text, we have now discovered phyB has an active function in FR light. Even striking is that the R and FR light exert an opposite effect. Thus, FR light is not simply nullifying the R effect but has an opposing effect to R light. What is more interesting is that the phyB-mediated actions of FR and R light occur at different cellular compartment of the plant cell, cytosol and nucleus, respectively, which was proven through utilization of the cytosolic and nuclear-localized mutant versions of phyB. Our observations thus shoot down a major dogma in plant physiology and will be considered highly provocative in phytochrome function. We argue that it would make much more sense that plants utilize the two isoforms rather than only one form, to effectively monitor the changing environmental light information and to incorporate the information into their developmental programs.

• Communications of Mathematical Education
• /
• v.27 no.1
• /
• pp.1-17
• /
• 2013

This work started with recent students' conception on Linear Algebra. We were trying to help their understanding of Linear Algebra concepts by adding visualization tools. To accomplish this, we have developed most of needed tools for teaching of Linear Algebra class. Visualizing concepts of Linear Algebra is not only an aid for understanding but also arouses students' interest on the subject for a better comprehension, which further helps the students to play with them for self-discovery. Therefore, visualizing data should be prepared thoroughly rather than just merely understanding on static pictures as a special circumstance when we would study visual object. By doing this, we carefully selected GeoGebra which is suitable for dynamic visualizing and Sage for algebraic computations. We discovered that this combination is proper for visualizing to be embodied and gave a variety of visualizing data for undergraduate mathematics classes. We utilized GeoGebra and Sage for dynamic visualizing and tools used for algebraic calculation as creating a new kind of visual object for university math classes. We visualized important concepts of Linear Algebra as much as we can according to the order of the textbook. We offered static visual data for understanding and studied visual object and further prepared a circumstance that could create new knowledge. We found that our experience on visualizations in Linear Algebra using Sage and GeoGebra to our class can be effectively adopted to other university math classes. It is expected that this contribution has a positive effect for school math education as well as the other lectures in university.

• Journal of The Korean Association For Science Education
• /
• v.32 no.2
• /
• pp.306-319
• /
• 2012

The purpose of this study was to investigate high school students' learning style, perception of types of laboratory instruction, and the relationship between the learning style and preferred type of laboratory instruction. The participants of this study were 19 female high school students, from Incheon in Korea, who showed outstanding science grades. Dunn et al's revised Learning Styles Inventory (1997) was used for testing students' learning style. The students were asked to choose preferred and not preferred types of experiments and describe the reason of the choices after implementation of 4 types of experiments: verification, discovery, explorator, and investigation laboratory instructions. The findings of this study were as follows: Firstly, the students had a certain common learning style irrelevant to their personal characteristics, i. e; they showed high (self, teacher, parents) motivation and structure stimuli that was common in Korean students. Second, outstanding students prefer unstructured and open laboratory instructions. And those students were highly influenced by sociological and emotional stimuli. Third, students' learning style was related to their preferred type of laboratory instruction. Students preferring unstructured and open laboratory were influenced by sociological and emotional stimuli, while those preferring structured and closed laboratory were influenced by physiological stimuli.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.36 no.3
• /
• pp.521-528
• /
• 2016

The tollbooths of a main motorway toll plaza are usually operated proactively responding to the variations of traffic demands of two-type vehicles, i.e. cars and the other (heavy) vehicles, respectively. In this vein, it is one of key elements to forecast accurate traffic volumes for the two vehicle types in advanced tollgate operation. Unfortunately, it is not easy for existing univariate short-term prediction techniques to simultaneously generate the two-vehicle-type traffic demands in literature. These practical and academic backgrounds make it one of attractive research topics in Intelligent Transportation System (ITS) forecasting area to forecast the future traffic volumes of the two-type vehicles at an acceptable level of accuracy. In order to address the shortcomings of univariate short-term prediction techniques, a Multiple In-and-Out (MIO) forecasting model to simultaneously generate the two-type traffic volumes is introduced in this article. The MIO model based on a non-parametric approach is devised under the on-line access conditions of large-scale historical data. In a feasible test with actual data, the proposed model outperformed Kalman filtering, one of a widely-used univariate models, in terms of prediction accuracy in spite of multivariate prediction scheme.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.4
• /
• pp.473-479
• /
• 2018

Objective: The study was designed to perform a genome-wide association (GWA) and partitioning of genome using Illumina's PorcineSNP60 Beadchip in order to identify variants and determine the explained heritability for the total number of teats in Yorkshire pig. Methods: After screening with the following criteria: minor allele frequency, $MAF{\leq}0.01$; Hardy-Weinberg equilibrium, $HWE{\leq}0.000001$, a pair-wise genomic relationship matrix was produced using 42,953 single nucleotide polymorphisms (SNPs). A genome-wide mixed linear model-based association analysis (MLMA) was conducted. And for estimating the explained heritability with genome- or chromosome-wide SNPs the genetic relatedness estimation through maximum likelihood approach was used in our study. Results: The MLMA analysis and false discovery rate p-values identified three significant SNPs on two different chromosomes (rs81476910 and rs81405825 on SSC8; rs81332615 on SSC13) for total number of teats. Besides, we estimated that 30% of variance could be explained by all of the common SNPs on the autosomal chromosomes for the trait. The maximum amount of heritability obtained by partitioning the genome were $0.22{\pm}0.05$, $0.16{\pm}0.05$, $0.10{\pm}0.03$ and $0.08{\pm}0.03$ on SSC7, SSC13, SSC1, and SSC8, respectively. Of them, SSC7 explained the amount of estimated heritability along with a SNP (rs80805264) identified by genome-wide association studies at the empirical p value significance level of 2.35E-05 in our study. Interestingly, rs80805264 was found in a nearby quantitative trait loci (QTL) on SSC7 for the teat number trait as identified in a recent study. Moreover, all other significant SNPs were found within and/or close to some QTLs related to ovary weight, total number of born alive and age at puberty in pigs. Conclusion: The SNPs we identified unquestionably represent some of the important QTL regions as well as genes of interest in the genome for various physiological functions responsible for reproduction in pigs.

• The KIPS Transactions:PartD
• /
• v.12D no.4 s.100
• /
• pp.627-636
• /
• 2005

The Intelligent Web Service is proposed for the purpose of automatic discovery, invocation, composition, inter-operation, execution monitoring and recovery web service through the Semantic Web and the Agent technology. To accomplish this Intelligent Web Service, the Ontology is a necessity for reasoning and processing the knowledge by the computer. However, creating service ontology, for the intelligent web service, has two problems not only consuming a lot of time and cost depended on heuristic of service developer, but also being hard to be mapping completely between service and service ontology. Moreover, the markup language to describe the service ontology is currently hard to be learned by the service developer In a short time. This paper proposes the efficient way of designing and creating the service ontology using MDA methodology. This proposed solution reuses the creating model in terms of desiEninE and constructing Web Service Model using UML based on MDA. After converting the Platform-Independent Web Service Model to the dependent model of OWL-S which is a Service Ontology description language, it converts to OWL-S Service Ontology using XMI. This proposed solution has profits, oneis able to be easily constructed the Service Ontology by Service Developers, the other is enable to be created the both service and Service Ontology from one model. Moreover, it can be effective to reduce the time and cost as creating Service Ontology automatically from a model, and calmly dealt with a change of outer environment like as the platform change. This paper cites an instance for the validity of designing Web Service model and creating the Service Ontology, and validates whether the created Service Ontology is valid or not.

• Parasites, Hosts and Diseases
• /
• v.36 no.2
• /
• pp.81-90
• /
• 1998

The present study was performed to observe the infection status of several kind of animals with indigenous Gnnthostomn in Korea, and morphological characteristics of gnathostome larvae detected from pit-viper, Askistronon breuiccudus, for the species bullfrogs. several kinds of snakes, j.e., 55 Elnphe rufodorsata, 2 Dinodon rufozonatum rufzonatum. 62 Rhnbdophis tisrinus tigrinus and 87 Askistronon app., and 438 cats were examined. A total of 21 larval gnathostomes was detected from 12 pit-vipers, A. breuicnudus. They were 2.233 x 0.343 mm in average size and covered with about 210 transverse rows of minute cuticular spines. Their characteristic head bulbs were provided with 4 rows of hooklets of which average numbers in each row were 36.8, 39.0, 41.7 and 44.3. posteriorly. In the cross sections of midgut level, the intestinal wall consisted of a single layer of 19-25 elongate epithelial cells with a single nucleus. SEM observation of the larvae revealed unique features of head bulb , cuticular spines on transverse striations and a cervical papilla. On the basis of above morphological characteristics, they were identified as the advanced third-stage larvae of Gnothostomo hispinum. It was first confirmed that the pit-viper. ABkistronon breuicaunus is the snake intermediate host of G. hispidun.