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The Growth Analysis of Sowing Times in Safflower(Carthamus tinctorius L.) (파종시기별 홍화의 생장해석)

  • Kim, Jae-Cheol;Ryu, Jung-Gi;Kim, San-Young;Park, Kyeng-Seok
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.47-51
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    • 2008
  • This study was carried out to get the basic data for safflower cultivation according to sowing date by growth analysis for 2 years form 2003 to 2004 at Institute of natural product in Uiseong, G.B.A.R.E.S.. Safflower(Cheongsoo cultivar) was seeded at March 1st, April 1st, May 1st, and July 1st in field. The necessary date for germination was 24 days, when safflower was seeded at March 1st, but it was 8 days which sowed at April 1st and when the seed was sowed at high temperature period, the necessary date for germination was getting short. The necessary date for flowering was the same trend as germination; it took 104 days at March 1st, 79 days at April 1st, 65 days at May 1st, and 58 days at July 1st sowing treatment. The safflower growth was the most vigor when it was sowed at March 1st. T/R(Top/Root) ratio was gently increased during growing season, but when the seed was sowed at July 1 st, it was low compare to sowing at March 1st and April 1st. RGR(Relative growth rate) was the highest during shoot growth stage, but it was rapidly decreased after flowering. CGR(Crop growth rate) was increased at branching stage, and the increasing velocity was slightly decreased just before flowering, and it was increased again at flowering and at this stage, CGR was the highest, and then it did not increased. NAR(Net assimilation rate) was the highest at shoot growth stage during growing season, and was the highest which was sowed at March 1st.

Development of Organic Paste Porcelain for Fixed Prostheses (유기조성물을 이용한 페이스트형 일반도재 시스템)

  • Han, Jung-Suk;Lee, Myung-Hyun;Kim, Dae-Hyun;Chung, Hun-Young
    • Journal of Dental Rehabilitation and Applied Science
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    • v.20 no.2
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    • pp.109-120
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    • 2004
  • INTRODUCTION: The build-up method has been used for application of porcelain powder on the metal framework to make final tooth shape conventionally. This method takes time and need skill to mimic final shade and shape of porcelain fused to metal crown. The purpose of this study was to develop standard shape and shade laminating porcelain forms to reduce build-up time. METHODS: To make tooth form porcelain paste, several liquid organic compounds were added to conventional feldspathic porcelain. The amount of additives and rheologic property were tested to find out best composition. Comparison of mixing methods to reduced porosity, proper heating schedule, and measurement of shrinkage amount and residual organic materials were performed to set-up standard procedures. Finally, biaxial flexural strength and color of preformed laminated paste porcelain were compared with those of porcelain which fabricated by the conventional build-up method. RESULTS: There was no significant difference in physical properties and color stability between two fabrication methods after various testing methods. Conclusion: This new build-up method can be applied to fabricate the PFM crown and bridge without any loss of strength and optical properties.

In vitro and In vivo Antibacterial Activity of a New Fluoroquinolone Containing C7-bicyclic Structure (C7-이환체 구조를 갖는 새로운 플루오르퀴놀론계 항생제의 in vitro와 in vivo 항균작용)

  • Han, Seung-Hui;Choe, Mun-Jeong;Kim, Ji-Yeon;Kim, Byeong-O;Sim, Jeom-Sun;Gang, Jin-Seok;Son, Ho-Jeong;Lee, Jae-Uk;Yu, Yeong-Hyo;Park, Myeong-Hwan
    • YAKHAK HOEJI
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    • v.40 no.4
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    • pp.428-437
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    • 1996
  • The in vitro and in vivo antibacterial activities of a new fluoroquinolone, DWP20364(1-cyclopropyl-5-amino-6,8-difluoro-7-(2,7-diazabicyclo[3.3.0]oto-4-ene-7-yl)-1 ,4-di-hydro-4-oxoquinoline-3-carboxylic acid) were evaluated in comparison with those of ciprofloxacin(CPFX), sparfloxacin(SPFX) and ofloxacin(OFLX). DWP20364 was more potent than CPFX and OFLX against Staphylococcus spp., Streptococcus spp. and Enterococcus faecium MD8b and it was similarly or slightly less active than CPFX against Escherichia spp. and Pseudomonas spp.. For MRSA and OFLX resistant strains (Staphylococcus spp.(14),Enterococcus spp.(4), Acinetobacter spp.(2), Pseudomonas spp.(9), Klebsiella spp.(2) and Serratia spp.(6)),DWP20364(MICs for 90% of strains,0.025 and 12.5${\mu}$g/ml, respectively) was 4 to 32 folds more potent than SPFX and CPFX. The activity of DWP20364 decreased moderately in the presence of 5mM $Mg^{2+}$. However, various pHs and the concentrations of various serum had no effect on the activity of DWP20364. DWP20364 possessed a bacteriocidal effect at the 1MIC against gram positive and gram negative strains. The protective effect of DWP20364 against systemic infections in mice caused by S. aureus Smith or S. aureus L2379 was superior to that of CPFX and SPFX but it was less active than that of CPFX against infection by P. aeruginosa E-2.

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The Effect of Salt Contents in Diet and Water on Performance and Physiological Changes in Broiler Chicks (염분 수준이 육계 병아리의 생산성과 생리적인 변화에 미치는 영향)

  • HwangBo J.;Hong E.C.;Lee B.S.;Lee H.J.;Jo Seong-Baek;Bae H.D.;Lee S.J.;Nho W.G.
    • Korean Journal of Poultry Science
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    • v.33 no.2
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    • pp.159-164
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    • 2006
  • To investigate the effects of salt contents in diet and water on the growth of broiler chicks, 288 heads of Hubbards strain chicks, were fed with com-soybean meal diets containing 0, 0.25, 0.5, 0.75 1.0, 2.0, 3.0, or 4.0% salt from 1d to l4d posthatching. And 180 chicks were also supplemented with salt to their water at 0, 0.1, 0.2, 0.5 or 1.0%. Feed intake and weight gain tended to decrease as the salt levels in diets increased and those were the lowest in 4% group as 481 g and 168 g, however, mortality was the highest in 4% group as 75.0%. As for the salt levels in water, feed intake and weight gain were low as 427 g and 162 g in 1% group, while mortality was 77.8%. Salt poisoning chicks were shown with thirsty, appetite deficiency, rising impossibility, sleepiness, or convulsions, and in autopsy, were shown with ascites, hydropericardium, cardiac hypertrophy, hemorrhage of eyelid, hemorrhage of trachea, hemorrhage of intestine, etc. Conclusively, salt content above 4% in diet or 1% in water could reduce the performance in broiler chicks.

Whole Genome Association Study to Detect Single Nucleotide Polymorphisms for Body Conformation Traits in a Hanwoo Population

  • Alama, M.;Lee, Y.M.;Park, B.L.;Kim, J.H.;Lee, S.S.;Shin, H.D.;Kim, K.S.;Kim, N.S.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.3
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    • pp.322-329
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    • 2011
  • A whole genome association (WGA) study was conducted to identify quantitative trait loci (QTL) for body conformation traits in Hanwoo cattle. The phenotypes of 497 steers were recorded from the Hanwoo Improvement Center of National Agricultural Cooperative Federation, Seosan, Korea, and analyzed using the Illumina Bovine 50 k SNP chip. A set of 35,987 SNPs that were available in the Hanwoo population was selected from the chip. After adjustments for the effects of year-season of birth, region and sire, phenotypes were regressed on each SNP using a linear regression model. Three hundred nineteen SNPs were detected for the ten conformation traits (p<0.003). For the significant SNPs, stepwise regression procedures were applied to determine best sets of markers. A total of 72 SNPs were selected (p<0.001), for which the sets of 5, 9, 10, 9, 8, 11, 4, 6, 3 and 7 SNPs were determined for height at withers, rump height, body length, chest depth, chest width, rump length, hip width, thurl width, pinbone width and heart girth, respectively. About 7-26% of the total phenotypic variation was explained by the set of SNPs for each trait. QTL for the conformation traits were harbored on most bovine chromosomes (BTAs). Four SNPs with pleiotropic effects on height at withers and rump height were detected on BTAs 3, 4, 6 and 16. A SNP with pleiotropic effects on chest width and rump length was also detected on BTA10. Two QTL regions, i.e. between 87 and 97 Mb in BTA3 and between 41 and 44 Mb in BTA7, were found, in which SNPs were detected for the five and three conformation traits, respectively. The detected SNPs need to be validated in other Hanwoo populations for commercial application to the genetic improvement of conformation characteristics in Hanwoo via marker-assisted selection (MAS).

Processing and Characteristics of Snacks Make from Extrusion Rice Oryza sativa and Dried Shrimp Acetes chinensis (마른 새우(Acetes chinensis) 첨가 Extrusion 쌀(Oryza sativa) Collet을 이용한 Snack의 제조 및 품질특성)

  • Je, Hae-Soo;Kang, Kyung-Hun;Jung, Hee-Bum;Park, Si-Young;Kang, Young-Mi;Seoung, Tae-Jong;Lee, Jae-Dong;Park, Jin-Hyo;Kim, Jeong-Gyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.3
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    • pp.293-300
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    • 2016
  • In the present study, we investigated the quality, sensory characteristics and commercialization potential of a rice collet snack made with the addition of dried shrimp. “Mild” and “spicy” snack products were produced with an edible oil coating and mixed seasoning powder coating, respectively. The approximate composition of the mild and spicy snacks, respectively, were 2.44% and 2.24% for moisture, 8.52% and 8.64% for crude protein, 18.36% and 26.54% for crude lipids, 1.28% and 1.38% for ash, 1.1% and 1.2% for salt, and 0.61 and 0.62 for pH. The L (lightness), a (redness), b (yellowness), and ⊿E (color difference) values were higher for the mild snack than the spicy snack. The mild and spicy snack had values of 7,776.4 and 7,655.8 mg/100 g for total amino acids, and 221.6 and 253.5 mg/100 g for total free amino acids, respectively. The TBA (thiobarbituric acid) value did not differ significantly between the two types of snack. The hardness value of the spicy snack was higher than that of the mild snack, but there were no significant differences in flavor between the two products. The sensory evaluation score of the spicy snack was slightly higher than that of the mild snack. Organoleptic inspection indicated that both snacks had a favorable, unique taste.

Prevalence and Genetic Characterization of Toxoplasma gondii in House Sparrows (Passer domesticus) in Lanzhou, China

  • Cong, Wei;Huang, Si-Yang;Zhou, Dong-Hui;Zhang, Xiao-Xuan;Zhang, Nian-Zhang;Zhao, Quan;Zhu, Xing-Quan
    • Parasites, Hosts and Diseases
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    • v.51 no.3
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    • pp.363-367
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    • 2013
  • The prevalence of Toxoplasma gondii infection in birds has epidemiological significance because birds are indeed considered as a good indicator of environmental contamination by T. gondii oocysts. In this study, the prevalence of T. gondii in 313 house sparrows in Lanzhou, northwestern China was assayed by the modified agglutination test (MAT). Antibodies to T. gondii were positive in 39 (12.46%) of 313 samples (MAT titer ${\geq}$ 1:5). Tissues of heart, brain, and lung from the 39 seropositive house sparrows were tested for T. gondii DNA, 11 of which were found to be positive for the T. gondii B1 gene by PCR amplification. These positive DNA samples were typed at 9 genetic markers, including 8 nuclear loci, i.e., SAG1, 5'- and 3'-SAG2, alternative SAG2, SAG3, GRA6, L358, PK1, c22-8 and an apicoplast locus Apico. Of them, 4 isolates were genotyped with complete data for all loci, and 2 genotypes (Type II variants; ToxoDB #3 and a new genotype) were identified. These results showed that there is a potential risk for human infection with T. gondii in this region. To our knowledge, this is the first report of T. gondii seroprevalence in house sparrows in China.

A Study on Estimation of Individual Growth Curve Parameters and their Relationships with Meat Quality Traits of Crossbred between Korean Native Boars and Landrace Sows (재래돼지와 랜드레이스 교잡종의 개체별 성장곡선 추정 및 육질형질과의 상관관계 추정에 관한 연구)

  • Cho, Y.M.;Choi, B.H.;Kim, T.H.;Lee,, J.W.;Lee, J.E.;Oh, S.J.;Cheong, I.C.
    • Journal of Animal Science and Technology
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    • v.46 no.4
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    • pp.503-508
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    • 2004
  • This study was conducted to estimate the growth curve parameters of crossbreds between Korean native boars and Landrace sows and their relationships with meat qualities. The data used were weight-age data and carcass data from 131 males and 122 females raised at N.L.R.I in Korea. Growth curve parameters were estimated from nonlinear regression using Gompertz model individually. Average mature weight(A), average maturing rate(k), and average inflection point(u) showing maximum growth rate estimated were 179.54${\pm}$6.06kg, 0.3154${\pm}$0.0059, and 5.50${\pm}$0.11 months in females, and 179.84${\pm}$6.33kg, 0.3049${\pm}$0.0061, and 5.24${\pm}$0.13 months in males, respectively. For the growth curve parameters and derived statistics, the phenotypic correlations of maturing rate with gain rate at inflection, mature weight, and inflection point were - .30, - .77, and - .93 in male, and - .31, - .78 and - .94 in female, respectively. Matrure weight was positively correlated to the inflection point as + .89 in both male and female, indicating that late maturing pigs with lower k had longer maturing period with increasing gain rate and reached point of inflection later than early maturing pigs with higher k, and grew to larger mature weight. Backfat thickness and erode fat contents were correlated with mature weight positively in male and negatively in female, and correlated with gain rate at inflection point positively in both male and female, of which coefficients were as high as .42 and .50 in male, respectively.

Agastache rugosa Leaf Extract Inhibits the iNOS Expression in ROS 17/2.8 Cells Activated with TNF-$\alpha$ and IL-$\beta$

  • Oh Hwa Min;Kang Young Jin;Kim Sun Hee;Lee Young Soo;Park Min Kyu;Heo Ja Myung;Sun Jin Ji;Kim Hyo Jung;Kang Eun Sil;Kim Hye Jung;Sea Han Geuk;Lee Jae Heun;YunChoi Hye Sook
    • Archives of Pharmacal Research
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    • v.28 no.3
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    • pp.305-310
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    • 2005
  • It has been suggested that nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) may act as a mediator of cytokine-induced effects on bone turn-over. NO is also recognized as an important factor in bone remodeling, i.e., participating in osteoblast apoptosis in an arthritic joint. The components of Agastache rugosa are known to have many pharmacological activities. In the present study, we investigated the effects of Agastache rugosa leaf extract (ELAR) on NO production and the iNOS expression in ROS 17/2.8 cells activated by a mixture of inflammatory cytokines including TNF-$alpha$ and IL-1$\beta$. A preincubation with ELAR significantly and concentration-dependently reduced the expression of iNOS protein in ROS 17/2.8 cells activated with the cytokine mixture. Consequently, the NO production was also significantly reduced by ELAR with an IC$_{50}$ of 0.75 mg/mL. The inhibitory mechanism of iNOS induction by ELAR prevented the activation and translocation of NF-$\kappa$B (p65) to the nucleus from the cytosol fraction. Furthermore, ELAR concentration-dependently reduced the cellular toxicity induced by sodium nitroprusside, an NO-donor. These results suggest that ELAR may be beneficial in NO-mediated inflammatory conditions such as osteoporosis.

Comparison of Opened Rates and Quality Characteristics of Frozen Baby-clam In-shell Tapes philippinarum Prepared by Different Processing Method (제조방법을 달리하여 제조한 껍질붙은 냉동바지락(Tapes philippinarum)의 껍질 개패율 및 품질특성 비교)

  • Park, Si-Young;Kang, Kyung-Hun;Lee, Jae-Dong;Yoon, Moon-Joo;Kang, Young-Mi;Seoung, Tae-Jong;Kweon, Su-Hyun;Choo, Yi-Kwon;Kim, Jeong-Gyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.6
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    • pp.743-749
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    • 2016
  • We compared two different processing methods for preparing high quality frozen in-shell baby clam products. In the first method, sand and mud were removed from the clams, then they were vacuum packed in polyethylene film, boiled at $97^{\circ}C$ for 6 min, and snap frozen in a cold air blast freezer (sample 1). The second processing method was similar, except the boiling process was excluded (sample 2). Both frozen products were boiled for 4 min, and then shucked and minced. Various quality metrics, such as the opening rates of shells, chemical composition, pH, volatile basic nitrogen (VBN), salinity, thiobarbituric acid (TBA), amino-N, total amino acids and free amino acids were measured, and sensory evaluation was conducted. The opening rates of shells of sample 1 and sample 2 were 98.3% and 4.67%, respectively. The proximate composition of sample 1 and sample 2 was 75.2% and 78.7% moisture, 19.7% and 16.2% crude protein, 2.45 and 2.2% crude lipid, 2.8% and 2.1% ash, and 2.1% and 1.9% salinity, respectively. The L, a, b and ${\Delta}E$ values were similar: 48.6 and 49.2, 3.9 and 3.9, 15.7 and 15.5, and 50.7 and 50.1 for sample 1 and sample 2, respectively. The sensory evaluation score of sample 1 was higher than that of sample 2. Sample 1 was deemed to be superior to sample 2; therefore, we determined that the boiling process is needed for manufacturing high-quality frozen clam products.