• 제목/요약/키워드: dye visualization

검색결과 61건 처리시간 0.034초

EXPERIMENTAL STUDY ON TURBULENT SWIRLING FLOW IN A CYLINDRICAL ANNULI BY USING THE PIV TECHNIQUE

  • Chang, T.H.
    • International Journal of Automotive Technology
    • /
    • 제5권1호
    • /
    • pp.17-22
    • /
    • 2004
  • An experimental investigation was conducted to study the characteristics of turbulent swirling flow in an axisymmetric annuli. The swirl angle measurements were performed using a flow visualization technique using smoke and dye liquid for Re=60,000-80,000. Using the two-dimensional Particle Image Velocimetry method, this study found the time-mean velocity distribution and turbulence intensity in water with swirl for Re=20,000, 30,000, and 40,000 along longitudinal sections. There were neutral points for equal axial velocity at y/(R-r)=0.7-0.75, and the highest axial velocity was recorded near y/(R-r)=0.9. Negative axial velocity was observed near the convex tube along X/(D-d)=3.0-18.0 for Re=20,000.

T형 볼밸브 내의 유동특성 해석 및 압력변동의 FFT 분석 (Study on the flow characteristics and FFT analysis to the pressure fluctuation in a T-type ball valve)

  • 여창호;허형석;서용권
    • 한국해양공학회:학술대회논문집
    • /
    • 한국해양공학회 2003년도 춘계학술대회 논문집
    • /
    • pp.240-244
    • /
    • 2003
  • In this paper, we present the CFD and experimental results for flow characteristics as well as pressure fluctuation in a T-type ball valve. In the experiment, flow visualization for the wake is performed with a fluorescent dye. In CFD, the valve flow is simulated three-dimensionally using a commercial fluid analysis code, FLUENT 6.0. FFT analysis to the pressure fluctuation in the wake region is also calculated Analysis results show that the characteristic frequency of the wake flow is strongly dependent on the Reynolds number.

  • PDF

An Experimental Study on Swirling Flow with Heat Transfer in the Horizontal Circular Annuli

  • Chang Tae-Hyun
    • Journal of Advanced Marine Engineering and Technology
    • /
    • 제29권3호
    • /
    • pp.260-274
    • /
    • 2005
  • An experimental investigation was performed to study the characteristics of turbulent swirling flow in the cylindrical annuli. The swirl angle measurements were performed by flow visualization technique using smoke and dye liquid. By using the particle image velocimetry method. this study has found the time-mean velocity distribution and turbulent intensity with swirl for Re=20,000. 30.000. 50.000. and 70,000 along longitudinal sections. The results appear to be physically reasonable. Other experimental study was performed to investigate heat transfer characteristics of turbulent swirling air flow in axisymmetric annuli. The static pressure. the local air flow temperature, and the wall temperature with decaying swirl were measured by using thermocouples and the friction factor and the local Nusselt number were calculated for Re=30,000. 50,000 and 70000. The local Nusselt number was compared with that obtained from the Dittus-Boelter equation with swirl and without swirl, respectively. The results showed that the swirl enhances the heat transfer at the inlet and the outlet of the test tube.

Site-Specific Labeling of Proteins Using Unnatural Amino Acids

  • Lee, Kyung Jin;Kang, Deokhee;Park, Hee-Sung
    • Molecules and Cells
    • /
    • 제42권5호
    • /
    • pp.386-396
    • /
    • 2019
  • Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing the subtle behavior of the protein and assessing its cellular function. Over the last decade, many strategies have been developed to achieve selective labeling of proteins in living cells. In particular, the site-specific unnatural amino acid (UAA) incorporation technique has gained increasing attention since it enables attachment of various organic probes to a specific position of a protein in a more precise way. In this review, we describe how the UAA incorporation technique has expanded our ability to achieve site-specific labeling and visualization of target proteins for functional analyses in live cells.

스텐트 삽입에 의한 방추형 동맥류 내부 유동의 변화 (Flow Changes by Stent Insertion in Fusiform Aneurysm Models)

  • 이계한;서남현
    • 대한의용생체공학회:의공학회지
    • /
    • 제22권6호
    • /
    • pp.535-542
    • /
    • 2001
  • 스텐트를 이용한 동맥류 색전술은 방추형 동맥류 등 목이 넓은 광경동맥류의 치료에 최근 사용되고 있다. 동맥류 내부의 혈류유동은 혈전의 형성 및 동맥류 폐색에 중요한 역할을 하므로 스텐트의 삽입으로 인한 동맥류 내부 혈류 유동의 변화를 고찰하기 위하여 광색성 염료를 이용한 유동가시화 방법을 사용하였다. 제작된 방추형 동맥류 모델에 대해 맥동 유동시 동맥류 벽 다섯 위치에서 유동장의 정성적 관탈 및 벽 전단변형률의 측정이 수행되었다. 스텐트의 삽입은 동맥류 내부의 유동을 감소시켰으며 동맥류 내부의 느린 와류유동이 감속후기가지 지속되었다. 또한 스텐트가 삽입된 모델은 스텐트가 없는 모델에 비하여 벽 전단변형률이 감소하였으며. OSI는 증가하였다 이러한 혈류유동의 변화는 혈전의 형성 및 내막거식증대가 일어나기 쉬운 혈류역학적 환경을 제공한다. 따라서 스텐트의 삽입으로 인한 동맥류 내부의 혈류 유동은 혈전의 형성 및 동맥류 폐색을 촉진하도록 변화함을 알 수 있었다..

  • PDF

엔진오일 누유 가시화 및 식별력 증대를 위한 기동화력장비 엔진 공정개선 (Process Improvement of Land System Engine for Visualization and Increasing Identification of Engine Oil Leakage)

  • 서석호;김지훈;오대산
    • 한국산학기술학회논문지
    • /
    • 제21권3호
    • /
    • pp.321-327
    • /
    • 2020
  • 최근 2년간 군 기동화력장비의 엔진 생산 공정 중에 발생한 주요 품질문제 중에는 엔진오일 누유가 가장 많은 비중을 차지하고 있다. 이를 최소화하기 위해서는 엔진오일 누유에 대한 근본적인 원인 파악과 다빈도로 발생되는 누유 부위에 대한 파악이 필요할 뿐만 아니라 더 나아가 품질비용 감소를 위해서는 엔진 조립 및 검사공정에서 누유에 대한 사전식별이 필요하다. 본 연구에서는 형광염료가 주입된 엔진오일을 통하여 누유를 가시화하고 엔진오일 누유 현상에 대한 식별성을 증대시켜 군 기동화력장비 엔진의 생산품질을 향상시키고자 하였다. 이를 위하여 기존에 사용하는 엔진오일과 형광염료가 주입된 엔진오일의 특성분석을 통하여 대체 가능성에 대해 검토하였으며 엔진오일 주입 공정과 누유검사 공정을 개선하여 엔진오일 누유에 의하여 발생할 수 있는 정비기간 및 시간을 최소화 시키고자 하였다. 최종적으로 본 연구결과를 토대로 군 기동화력장비 엔진의 신뢰도 확보 및 엔진 생산업체의 품질관리 능력 향상을 통하여 엔진 생산 공정간 발생할 수 있는 품질비용을 최소화 시킬 수 있다고 사료된다.

Influence of Design of Turbulence Generator on Flow Behavior in Pilot Headbox

  • Youn Hye Jung;Lee Hak Lae;Chin Seong Min
    • 펄프종이기술
    • /
    • 제36권5호
    • /
    • pp.1-10
    • /
    • 2004
  • The geometry of headboxes is of great importance in obtaining good formation, even basis weight profile and fiber orientation. Therefore, many attentions have been made to examine the influence of the geometry of headboxes on the flow behavior. In this study, to evaluate flow behavior in headboxes, three types of turbulence generators were examined using pilot headbox. Velocity profiles in MD and CD were measured using a pressure monitoring system and flow in headboxes was visualized by dye injection method. CD velocity profiles at three different locations inside the slice of Type A headbox showed that the velocity increased downstream to slice exit and had a pattern with four humps due to the persisting wall effect of step diffusors. Results from the evaluation of normalized velocity profile and flow visualization showed that L-shaped Type C headbox caused a large pressure drop but it lacked in flow stabilizing ability.

CAD를 이용한 패션 일러스트레이션의 회화적 입체표현에 관한 연구 (Solid Graphic Expression in Fashion Illustration Using CAD)

  • 신상무;박영옥
    • 복식
    • /
    • 제44권
    • /
    • pp.131-141
    • /
    • 1999
  • The purpose of this study was to investigate the various expressions with materials in fashion illustration using CAD comparing with hand work. The design motive for this study is to be selected from Bell Epoque era which was the revolutionary period in fashion illustration. The results of this study were as follows: 1. By using CAD, water color was well expressed to repeat brushing for the clarity, darkness, and brightness. It was more effective to use CAD in layering or duplicating complex and intricate patterns because the base color gets to be concealed under the repeated oil pastel. Acryl, like oil pastel, was easily absorbed in canvas, so it is effective to repeat brushing for expressing pure color. It was inconvenient to use wax crayon for controlling the moderate opacity because wax crayon absorbed water color dye stuffs, so crayon line was concealed when repetitions were being done. 2. The advantage of using CAD was convenience for getting rid of troublesome process and inefficient works. Also, CAD had a good tool like oil pastel in the use of coloring work by using pure color. By using CAD, various expressions on materials and texture of surface can be achieved effectively. Also, it is very strong substitute for time-saving, convenience, economic aspects from providing simple instrument, and production in the state of various kinds of paper and canvas as a method of visualization. Therefore, fashion illustration using CAD provides effective way of producing works, and gives promising vision in the future.

  • PDF

마이크로 채널 내부의 Micro-LIF 측정을 위한 마이크로 레이저 평면빔과 혼합용매의 적용 (Application of Micro-Thin Laser sheet and Mixed Solvent for Micro-LIF Measurement in a Microchannel)

  • 윤상열;김재민;김경천
    • 한국가시화정보학회:학술대회논문집
    • /
    • 한국가시화정보학회 2004년도 추계학술대회 논문집
    • /
    • pp.86-89
    • /
    • 2004
  • One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1$ to 3(3 or 5(5 pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

  • PDF

접촉 표면의 강성 변화에 따른 박테리아의 군집 패턴 (Bacterial Pattern Formation in Response to the Stiffness of Substrates)

  • 김중경;박은정;김성래;조명옥;한화택
    • 한국가시화정보학회:학술대회논문집
    • /
    • 한국가시화정보학회 2007년도 추계학술대회
    • /
    • pp.88-91
    • /
    • 2007
  • Animal cells show different behaviors in response to the mechanical properties of the substrates. We hypothesize that the rigidity of the substrates also affects the bacterial motility and controls the colony dynamics. It is found that the colony size of Escherichia colis and Bacillus subtilis grown on the agar plates is correlated with agarose gel concentrations and thus with the substrate rigidity. High- resolution microscopic imaging reveals that bacteria in single colonies form different aggregation patterns on the agar plates with varying gel concentration. We measured the apparent diffusion coefficients in the agarose gel plates made with different gel concentrations. Mathematical modeling and quantitative imaging of dye dispersion in the agar plates suggest that there is a close connection between the diffusion rate and the colony size. Nanoscale pore structures and kinetic constraints in the porous media may have an effect on bacterial colony dynamics.

  • PDF