• Clinical and Experimental Reproductive Medicine
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• v.32 no.1
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• pp.17-26
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• 2005

Objective: Preimplantation genetic diagnosis (PGD) is reserved for couples with a risk of transmitting a serious and incurable disease, and hence avoids the undesirable therapeutic abortion. In this study, we evaluated the efficacy of PGD for Duchenne muscular dystrophy (DMD) cases by the fluorescent PCR with polymorphic linked markers and the conventional duplex-nested PCR methods. Methods: Biopsy of one or two blastomeres was done from the embryos fertilized by ICSI on the third day after fertilization. We performed two cases of PGD-DMD by the duplex-nested PCR for the causative mutation loci and the SRY gene on Y chromosome. The triplex fluorescent PCR for the mutation loci, the SRY gene and the polymorphic microsatellite marker on X chromosome was applied for two cases of PGD-DMD. Results: By the duplex-nested PCR, successful diagnosis rate was 95.5% (21/22), but we could not discriminate the female embryos whether normal or carrier in this X-linked recessive disease. However, the triplex fluorescent PCR method showed 100% (27/27) of successful diagnosis rate, and all female embryos (n=17) were distinguished normal (n=10) from carrier (n=7) embryos. Unaffected and normal embryos were transferred into mother's uterus after diagnosis. A healthy normal male was achieved after PGD with the duplex-nested PCR method and a twin, a male and a female, were delivered with triplex fluorescent PCR method. The normality of dystrophin gene was confirmed by amniocentesis and postnatal genetic analysis in all offsprings. Conclusion: The fluorescent PCR with polymorphic marker might be useful in improving the specificity and reliability of PGD for single gene disorders.

• The Journal of the Acoustical Society of Korea
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• v.36 no.3
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• pp.211-217
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• 2017

In this paper, we propose a bidirectional full duplex MAC (Medium Access Control) protocol for underwater acoustic networks. An underwater sensor node can set a back-off timer according to the priority of transmission. When the back-off timer expires, the underwater sensor node acquires a transmission opportunity. If a source node wants to send data to a destination node, it broadcasts RTS (Request-To-Send) including ID of the destination node to neighbor nodes. The destination node receiving RTS sends CTS (Clear-To-Send) to the source node to inform the bidirectional full duplex communication. After the source node receives CTS, the source node and the destination node can send the data to each other. In the underwater environment, the existing MAC protocol may take a lot of time for successful transmission of data due to long underwater propagation delay. On the other hand, the proposed bidirectional full duplex MAC protocol improves the throughput by shortening the time for successful transmission of data. In this paper, we analyze the throughput of the proposed bidirectional full duplex MAC protocol. In addition, we show that the proposed bidirectional full duplex MAC protocol has better performance in the presence of the long underwater propagation delay compared with existing MAC protocols for underwater environments.

• Korean Journal of Microbiology
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• v.54 no.2
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• pp.98-104
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• 2018

Naegleria fowleri and Acanthamoeba spp. are free-living amoebas that are widely distributed in natural environments. Although uncommon, infection with these protozoans can cause fatal disease in humans and animals. In this study, in order to select the appropriate method to survey Naegleria fowleri and Acanthamoeba spp. in water samples, four molecular biology techniques and one commercially available kit for real-time PCR were compared. The results indicated that the duplex real-time PCR was the most sensitive, and could be used to simultaneously detect two different free-living amoebas. Using the duplex real-time PCR approach, the two free-living amoebas were surveyed in three local streams in Daejeon, Republic of Korea. The concentrated free-living amoebas were inoculated onto non-nutrient agar plates which had been spread with heat-inactivated Escherichia coli and incubated for 5~7 days. After incubation, gDNA was extracted and used as the template for amplification by duplex real-time PCR. Acanthamoeba spp. and N. fowleri was detected from ten (83.3%) and two (16.6%) of the twelve samples, respectively. As these two free-living amoebas can be fatal, continuous surveillance is needed to track their distribution in the aquatic environment for the drinking water safety.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.5
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• pp.436-442
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• 2013

The diversity-multiplexing tradeoff (DMT) function of a special half-duplex (HD) dynamic decode and forward (DDF) relay protocol with more than two antennas at the source node, two antennas at the relay node, and two antennas at the destination node is derived. This protocol is compared to a HD NAF protocol with the same number of source antennas and a HD DDF relay protocol with two antennas at the source node, more than two antennas at the relay node, and two antennas at the destination node.

• ETRI Journal
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• v.40 no.2
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• pp.207-217
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• 2018

As implementation of the in-band full duplex (IFD) transceiver becomes feasible, research interest is growing with respect to using IFD communication with cellular networks. However, the cellular network in which the IFD communication is applied inevitably suffers from an increase of the co-channel interference (CCI) due to IFD simultaneous transmission and reception. In this paper, we analyze the performance of a cellular network based on uni-directional IFD (UD-IFD) communication, wherein an IFD base station simultaneously supports downlink and uplink transmissions of half-duplex (HD) users. In addition, a multi-pair CCI cancellation (MP-CCIC) method combining CCIC and user pairing is proposed to improve the performance of the UD-IFD network. Simulation results showed that, compared to a conventional HD cellular network without using CCIC, capacity gain was not obtained in the UD-IFD cellular network. On the other hand, when applying the proposed MP-CCIC, the capacity of the UD-IFD cellular network greatly improved compared to that of an HD cellular network.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.11
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• pp.1374-1379
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• 2016

In almost all schemes of cooperative cognitive radio networks (CCRN), the users transmit and receive signals in half-duplex mode. In this paper, a design of CCRN adopting the full-duplex (FD) technique is addressed. In order to enable FD communication among users in the CCRN, simultaneous transmitting and receiving antennas are employed for the secondary users. Preliminary results from analysis and numerical evaluation indicate that the proposed FD multiple-input-multiple-output CCRN framework can provide a performance gain over the conventional CCRN frameworks.

• Korean Journal of Materials Research
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• v.24 no.11
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• pp.610-616
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• 2014

In this study, we investigated the precipitation behavior of the R-phase precipitated at the initial stage of aging and its effect on the pitting corrosion of 25%Cr-7%Ni-4%Mo super duplex stainless steel. The R-phase in super duplex stainless steel was mainly precipitated at the interface of ferrite/austenite phases and inside of the ferrite phase during the initial stage of aging, and it was transformed into the ${\sigma}$-phase with an increase in aging time. The ferrite phase was decomposed into a new austenite phase and ${\sigma}$-phase. The R phase was an intermetallic compound, which represented a lower Ni and higher Mo than the matrix, and also had a higher Mo and Cr concentration than the ${\sigma}$ phase. With an increasing aging time, the pitting potential $E_p$ was increased slowly by the precipitation of the R-phase, and it was then steeply decreased by the precipitation of the ${\sigma}$-phase. The R-phase was decreased the pitting potential, but its effect was smaller than effect of ${\sigma}$-phase.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.8
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• pp.921-923
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• 2016

In this letter, massive MIMO and full-duplex communication are considered together for high efficiency next generation WLAN systems. The proposed scheme allocates different carrier sensing thresholds by applying the joint spatial division and reuse (JSDR) scheme and is able to enhance the efficiency of MU-MIMO protocols by reducing the protocol overhead. Finally, full-duplex communication is applied to improve the spectral efficiency of WLAN systems.

• The Plant Pathology Journal
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• v.30 no.4
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• pp.445-449
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• 2014

The incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan) in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the management of viral infections that could undermine yield.

• Journal of Ocean Engineering and Technology
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• v.24 no.5
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• pp.55-59
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• 2010

The effect of aging on the precipitation of the $\sigma$ phase and corrosion resistance in W-substituted, super-duplex stainless steel was investigated. The volume fraction of the $\sigma$ phase and the current density increased as the aging temperature increased up to $750^{\circ}C$, and, then, they decreased. As aging time increased, the volume fraction of the $\sigma$ phase and the current density also increased. The $\sigma$ phase considerably influenced to corrosion resistance. When Mo was substituted for W in super-duplex stainless steel, the volume fraction of the $\sigma$ phase and the current density both decreased markedly.