• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.8
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• pp.1077-1082
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• 2007

In this study, killer yeasts were isolated from traditional Nuruk to improve storage and suppress contaminant in food industry. Among killer yeasts, yeast K15 showed strong killer toxin activity and inhibited growth of Salmonella Typhimurium and Vibrio parahaemolyticus. Killer yeast K15 was identified with Pichia anomala by the Microlog TM 4.0 identification system and homology of the ITS sequence. Killer toxin generated from P. anomala K15 was inactivated by pronase E and suggested to be a protein. Therefore killer toxin of P. anomala K15 was thought to be safe in human such as bacteriocin. P. anomala K15 was sufficient for growth in 50% glucose and could be used to prevent contaminant in initial stages of alcohol beverage fermentation.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.2
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• pp.69-77
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• 2007

We examined the depigmentation effect on Korean traditional citrus 17 species. With B16 melanoma cells, we have seen inhibition of the tyrosinase and melanin formation, which eventually were dose dependently decreased by three citrus fruits, immature Citrus unshiu, Citrus hassaku, and Citrus sinensis ${\times}$ reticulata as compared with positive control. Also, we examined expression of tyrosinase, DOPAchrome tautomerase (TRP-2), and DHICA oxidase (TRP-1) which affect melanin synthesis. Especially, immature Citrus unshiu decreased the protein levels of tyrosinase and TRP-1. In conclusion, immature Citrus unshue showed the strongest activity in all the experiments mentioned above and we expect that it can be used for preventing UV-induced pigmentation.

• Journal of Fisheries and Marine Sciences Education
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• v.21 no.4
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• pp.562-567
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• 2009

This study was conducted to evaluate of improving extruded pellet (EP) for growth of juvenile flounder by comparing with raw fish-based moist pellet (MP) diet. Two replicate groups of 80 fish per each tank (initial weight of $36.0{\pm}1.2 g$) were fed one of the three EPs (EP1, EP2 and CEP) and (MP for 16 weeks. Survival was not significantly affected by experimental diets (P>0.05). Weight gain of fish fed the MP was not significantly different from that of fish fed the EP1 & EP2 (P>0.05), but that of fish fed CEP was significantly lower than that of fish fed MP (P<0.05). Specific growth rate of fish fed the CEP was significantly lower than those of fish fed EP1, EP2 and MP (P<0.05). Feed efficiency of fish fed the EP1 was significantly higher than those of fish fed CEP and MP (P<0.05), but not significantly different from those of fish fed EP2 (P>0.05). The Contents of crude protein and crude lipid in whole body were significantly different among all groups (P<0.05). Therefore, these results strongly suggest that EP1 & EP2 diets could be developed to replace MP diet for flounder without adverse effects on growth performance.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.3
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• pp.667-675
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• 2014

Two types of diets, extruded pellet (EP) and raw-fish-based moist pellet (MP) were fed to compare growth, water quality and histology of olive flounder, Paralichthys olivaceus in Jeju fish farm. Two groups of 7000 fish per each tank (initial mean weight 51 g) were fed one of two diets for 7 months. At the end of the feeding trial, significant differences were found in weight gain and feed utilization. Dissolved oxygen (DO) level of the MP tank was decreased from 7.98 to 6.89 after feeding. There were no significant differences in hematocrit, hemoglobin, alanine aminotransferase and aspartate aminotransferase of fish fed the two diets. Whole body protein content of fish fed the EP was significantly higher than that of fish fed the MP. While the feeding rate by month indicated numerically decreased, fish fed the MP had higher feed intake than that of fish fed the EP. Histological examination of hepatopancreas, kidney, and anterior intestine of fish fed the EP did not show any negative effects compared to those of fish fed the MP. This study revealed that EP could substitute for MP in terms of growth performance, blood parameters, water quality and histology of olive flounder.

• Animal cells and systems
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• v.2 no.2
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• pp.249-258
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• 1998

Obesity, one of the most common metabolic diseases in industrial countries is characterized by an increase in the number or size of adipocytes. In an effort to create transgenic mouse models for the study of obesity we developed a novel technique in which adipose tissue can be ablated genetically at will, at any specific developmental stage and/or physiological condition, by the treatment of ganciclovir. We made a series of adipocytespecific expression vectors using minimal regulatory regions of brown adipocyte-specific uncoupling protein (UCP-1) gene and adipocyte-specific aP2 gene, and then analyzed their expression characteristics in cultured cell lines. When both constructs pUCP-LacZ and paP2-LacZ were transfected transiently into differentiating 3T3-L1 (pre-while adipocytes) and HIB-1B (pre-brown adipocytes) cell lines in vitro and then monitored by X-gal staining of cells, these regulatory regions were sufficient to show proper differentiation stage-specific expression in adipocvtes. To confirm that adipocytes expressing HSV-TK controlled by these minimal requlatory elements are sufficient to kill themselves with ganciclovir treatment pUCP-TK and paP2-TK expression constructs were transfected stably into HIB-1B and 3T3-L1 cells, respectively, and their ganciclovir sensitivities were tested during in vitro differentiation of cells. As expected more than 80% of cells were dead by the 7th day of treatment with ganciclovir while negative control cells were not affected at all. The data suqqest that the constructed vectors are suitable for obtaining novel obese transqenic models based on a conditional genetic tissue ablation method.

• Journal of the Korean Society of Food Culture
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• v.12 no.3
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• pp.289-299
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• 1997

This study was performed with survay in the field and literature. The result were; 1. In Vietnam, They took their meal in three times a day. They have taken Nuoc nam (fish sauce) with sliced red pepper, half a lime, etc... in every day every night. Fish Sauce was prepared with small fish (generally fresh- water fish), the same amount of salt, and stored in 8 months, and made filltered fluid. It's Nuoc mam. They have gained protein sauce from it. 2. Their basic menu were composed with rice, soup, food with marine products and meat (generally pork), various vegetables, tropical fruit. 3. Food of cereals were Com Trang (white rice), Pho (rice noodle), Chao (rice gruel), Banh Trang (rice paper), Banh mi (bread) etc... Food of meat were Ho sua, Banh bao chien (barbecued little pork), Suon Nuon (grilled pork), Cha Lua (sausage of pork) etc..., Thit be nhung (grilled beef, Dog meat, Chicken, Duck, Frog. Food of fish were generally fresh-water fish, Ca Chien (grilled fish), Canh chua (soup with sour taste), Ca chem chung (steamed fish with fragrant vegetable), Lobster, Crab, Oyster, Cuttlefish, Shellfish, etc... Food of vegetables were Doa Hanh (Kimchi with a welsh onion), Rau xao hon hop (roasted vegetables), Goi Tom (salad), Canh he dau hu (soup) etc..., and They took much food of trophical fruit, Tra (Tea), Coffee, Lua Moi (distilled liquor). 4. For example, Their Daily meal were composed of Sup Bong Ca (Soup), Heo Sua, Banh Bao Chien (barbecued little Pork), Top Hap (steamed shrimp), Cua (steamed Crab), Luon Um (bioled a fresh-water eel), Lau Thap Cam, Hai Sam Sac Nam Dong Co, Trai Cay.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.21 no.1
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• pp.87-91
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• 1976

This study was undertaken to learn the over-all influence of fertilizer on soybean culture and the chemical composition of soybean in the reclaimed land. Treatments included five rates of N.P.K: 4.10.8, compost: 1, 000. N.P.K.: 4.10.8 + compost: 1, 000, N.P.K: 2.5.5 kg/10 are as well as unfertilized plot in the reclaimed land and N.P.K: 4.10.8 kg/10 are as a check plot of the cultivated area. The yield of green plant above-ground, grain yield and protein content were higher in the cultivated plot than in the reclaimed land. Maximum yield of green plant or the greatest grain yield occurred in the plot of N.P.K: 4.10.8+compost: 1, 000 kg application and they diminished as the fertilizer application decreased. Yet there was no statistical significance in the chemical seed composition among treatments.

• Journal of Veterinary Clinics
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• v.10 no.1
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• pp.141-145
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• 1993

The present study was performed in order to clarify the significance of serum amyloid A(SAA) estimation for the diagnosis of bovine amyloidosis and SAA as a useful parameter for the health status in herds. Twelve dutch dairy cows with final diagnosis(2 with amyloidosis, 3 with acute inflammatory disease and 7 with chronic inflammatory disease) were used to charify the significance of SAA determination for the diagnosis of bovine amyloidosis. The SAA concentration in the group of inflammatory disease was higher than that of amyloldotic group. Further the SAA value in the group of acute inflammatory disease was higher than that of chronic ones. To clarify the significance of SAA estimation as a useful parameter for the health status in herds, two Korean dairy farms(A and B) were selected and the SAA concentration was determined in total 76 cows(49 from A farm and 27 from B farm). The SAA concentration in cows from A farm was ranged with 0～169%. The cows with high level of SAA(31～169%) had the disease histories(1 with retained placenta, 3 with chronic mastitis, 2 with acute mastitis, 1 with abortion and acute mastitis, 1 with ovarian dysfunction, 1 with downer cow syndrome and 1 with laceration of the teat). The SAA value in the cows from B farm was ranged with 0～29% and disease history was not detected. In conclusion the SAA determination only is thought to be difficult for the diagnosis of bovine amyloidosis. Furthermore SAA estimation is thought to be a useful parameter for the health status in herds.

• Korean journal of food and cookery science
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• v.24 no.2
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• pp.243-250
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• 2008

This study examined the quality characteristics of soybean pastes that were prepared using different ratios of whole soybean meju inoculated with three different types of microbial strains. The five samples were prepared with meju inoculated with Aspergillus oryzae KFRI 995, Bacillus subtilis F4005, mixture with A. oryzae KFRI 995 and B. subtilis F2315, mixture with A. oryzae KFRI 995 and B. subtilis F4005, and mixture using all three strains, respectively. Over two months of fermentation, pH gradually decreased, whereas total acidity increased. And across samples and fermentation periods, there were no considerable changes in moisture or crude protein. In all five samples, amino-type nitrogen levels increased approximately 2- to 3-fold as compared to initial levels; the sample inoculated with A. oryzae KFRI 995 showed the highest level (205.2 mg%) of amino-type nitrogen. Throughout fermentation, the five samples displayed differences in their reducing sugar levels according to the inoculated microorganisms. The lowest level of lightness and highest level of redness occurred in the sample inoculated with B. subtilis F2315 showed a lowest level in lightness and a highest level of redness, indicating a strong browning reaction. Finally, the samples inoculated with A. oryzae KFRI 995 and B. subtilis F4005, respectively, showed had similarly high preference scores as compared to the other samples.

• The Korean Journal of Food And Nutrition
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• v.32 no.5
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• pp.434-441
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• 2019

In this study, we analyzed the nutritional composition properties of soybeans and the organic acids, amino acids and volatile flavor compounds of fermented soybean products. We used five soybean cultivars including Pyeongwon, Jinpung, Saedanbaek, Saeolkong and Cheonga for this experiment. Physicochemical analysis of soybeans, showed that the cured protein and fat contents were 35.12~45.12 and 14.26~20.14%, respectively. The rank order of major organic acids was lactic acid > acetic acid > fumaric acid, with Saedanbaek being the highest. Total amino acid content of the samples was 358.12~657.28 mg/100 g, and glutamic acid, alanine, cysteine, valine, leucine, histidine and arginine were the major amino acids. We identified a total of 34 volatile aroma-compounds, including 7 alcohols, 7 acids, 7 ketones, 5 phenols, 2 esters, 1 furan, 4 pyrazines, and 1 miscellaneous compounds. As a result of this, could be applied to determine the suitability of cultivars and the quality for the process of the fermented soybean products.