• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9355-9360
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• 2014

Background: Oral squamous cell carcinoma (OSCC) remains as one of the most difficult malignancies to control because of its high propensity for local invasion and cervical lymph node dissemination. In this study, we evaluate the efficacy of our novel pH and temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) in affecting HER2 expression profile in OSCC model in rat. Results: DOX-MTX- nanoparticle complexes caused significant decrease in mRNA level of HER2 compared to untreated cancers (p<0.05) and this finding was more pronounced with the IV mode (p<0.000). Surprisingly, HER2 mRNA was not affected in DOX treated as compared to the control group (p>0.05). On the other hand, in the DOX-MTX NP treated group, fewer tumors characterized with advanced stage and decreased HER2 paralleled improved clinical outcome (P<0.05). Moreover, the effectiveness of the oral route in the group treated with nanodrug accounted for the enhanced bioavailability of nanoparticulated DOX-MTX compared to free DOX. Furthermore, there was no significant difference in mRNA level of HER2 (p>0.05). Conclusions: Influence of HER2 gene expression is a new feature and mechanism of action observed only in dual action DOX-MTX-NPs treated groups. Down-regulation of HER2 mRNA as a promising marker and prognosticator of OSCC adds to the cytotoxic benefits of DOX in its new formulation. Both oral and IV application of this nanodrug could be used, with no preferences in term of their safety or toxicity. As HER2 is expressed abundantly by a wide spectrum of tumors, i DOX-MTX NPs may be useful for a wide-spectrum of lesions. However, molecular mechanisms underlying HER2 down regulation induced by DOX-MTX NPs remain to be addressed.

• Advances in aircraft and spacecraft science
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• 제7권6호
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• pp.553-570
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• 2020

To address the problems caused by the strong coupling of an airbreathing hypersonic vehicle's airframe and propulsion to the integrated control system design, an integrated airframe-propulsion model is established, and the coupling characteristics between the aircraft and engine are analyzed. First, the airframe-propulsion integration model is established based on the typical nonlinear longitudinal dynamical model of an air-breathing hypersonic vehicle and the one-dimensional dual-mode scramjet model. Thrust, moment, angle of attack, altitude, and velocity are used as transfer variables between the aircraft model and the engine model. The one-dimensional scramjet model can accurately reflect the working state of the engine and provide data to support the coupling analysis. Second, owing to the static instability of the aircraft model, the linear quadratic regulator (LQR) controller of the aircraft is designed to ensure attitude stability and height tracking. Finally, the coupling relationship between the aircraft and the engine is revealed through simulation examples. The interaction between vehicle attitude and engine working condition is analyzed, and the influence of vehicle attitude on engine safety is considered. When the engine is in a critical working state, the attitude change of the aircraft will not affect the engine safety without considering coupling, whereas when coupling is considered, the attitude change of the aircraft may cause the engine unstart, which demonstrates the significance of considering coupling characteristics.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2003년도 춘계종합학술대회
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• pp.155-160
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• 2003

본 논문에서는 동공 검출을 이용한 얼굴 검출 방법을 제안하고자 한다. 동공검출기는 눈의 역반사 특성을 이용한 능동적 조명을 사용하여 검출을 용이하도록 하였고. 제안된 방법의 검출 범위는 소형컴퓨터나 무인정보 단말기 등의 활용에 적합하도록 하였다. 동공 후보 영역을 계산하여 검출한 후, 학습 규칙을 사용하여 얼굴에 대응하는 두 개의 지점을 선택하도록 하였다. 얼굴 검출 성능을 증명하기 위하여, 얼굴의 최대 돌출 부위를 지정 할 수 있는 이중 모드의 얼굴 추적 장치를 개발하였다. 추정치와 복합 얼굴 검출기의 측정치를 결합하여 특징 관계를 추적함으로 처리의 안정성을 높였으며 또한, 실시간으로 서보의 제어 장치를 원격 조정하여 항상 카메라가 이미지의 중앙을 추적하도록 함으로서 얼굴의 위치를 추정할 수 있도록 하였다.

• Structural Engineering and Mechanics
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• 제47권6호
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• pp.881-898
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• 2013

This paper describes analytical investigation into a new dual function system including a couple of shear links which are connected in series using chevron bracing capable to correlate its performance with magnitude of earthquakes. In this proposed system, called Chevron Knee-Vertical Link Beam braced system (CK-VLB), the inherent hysteretic damping of vertical link beam placed above chevron bracing is exclusively utilized to dissipate the energy of moderate earthquakes through web plastic shear distortion while the rest of the structural elements are in elastic range. Under strong earthquakes, plastic deformation of VLB will be halted via restraining it by Stopper Device (SD) and further imposed displacement subsequently causes yielding of the knee elements located at the bottom of chevron bracing to significantly increase the energy dissipation capacity level. In this paper first by studying the knee yielding mode, a suitable shape and angle for diagonal-knee bracing is proposed. Then finite elements models are developed. Monotonic and cyclic analyses have been conducted to compare dissipation capacities on three individual models of passive systems (CK-VLB, knee braced system and SPS system) by General-purpose finite element program ABAQUS in which a bilinear kinematic hardening model is incorporated to trace the material nonlinearity. Also quasi-static cyclic loading based on the guidelines presented in ATC-24 has been imposed to different models of CK-VLB with changing of vertical link beam section in order to find prime effectiveness on structural frames. Results show that CK-VLB system exhibits stable behavior and is capable of dissipating a significant amount of energy in two separate levels of lateral forces due to different probable earthquakes.

• 드라이브 ㆍ 컨트롤
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• 제17권3호
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• pp.33-46
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• 2020

We introduce a piece of special-purpose equipment for responding to disasters that has a dual-arm manipulator consisting of six-axis multi joints, and a master-slave operating system controlled by a wearable joystick for intuitive and convenient operation. However, due to the complexity and diversity of a disaster environment, training and suitable training means are needed to improve the interaction between the driver and equipment. Therefore, in this paper, a system that can improve the operator's immersion in the training simulation is proposes, this system is implemented in a virtual environment. The implemented system consists of a cabin installed with the master-slave operation system, a motion platform, visual and sound systems, as well as a real-time simulation device. This whole system was completed by applying various techniques such as a statistical mapping method, inverse kinematics, and a real-time physical model. Then, the implemented system was evaluated from a point of view of the appropriateness of the mapping method, inverse kinematics, the feasibility for real-time simulations of the physical environment through some task mode.

• 항공우주정책ㆍ법학회지
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• 제3권
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• pp.233-258
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• 1991

This paper examines current developing status of space law as defined in terms of its unique principle that "the utilization of outer space for all mankind's interest". As commercialization of space activities has brought about heated debate on its legitimacy with respect to that principle, space law has come to witness a dual situation. One is the realization of that principle in establishing commercial space activities system where all mankind's interest is respected through the non-discriminatory distribution mode of space benefit. In satellite telecommunication system, the INTELSAT Organization assures its public service policy while protecting itself against a competition from separate systems. For remote sensing, commercialization of LANDSAT Systems promoted since 1984 seems not to affect present non-discriminatory distribution of data and information obtained. On the other hand, active participation of private entity aiming at commercial profit enables national government to manage more effective control and supervision of those activities with a view to promoting national interests. Also, newly developed private rules and regulations are emerging in business relations about commercial space activities. Extended capacity of national government assuring its national interest in production and distribution of international resources that is outer space, and regulation of space activities by newly developed rules and customs provoke a concern about a identity problem of space law. The best way to summarize this perspective might consist in saying that non space law regulations, which are not assumed as aiming at the promotion of all mankind's interest, are confirming their role about space related activities. For those reasous, we are now facing a developing status of space law as developments of commercial space activities continues.

• 한국정보통신학회논문지
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• 제7권8호
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• pp.1823-1830
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• 2003

본 논문에서는 동공 검출 기술을 이용한 얼굴 검출 방법을 제안하고자 한다. 동공 검출기는 눈의 역 반사 특성을 이용한 능동적 조명을 사용하여 검출을 용이하도록 하였고. 제안된 방법의 검출 범위는 소형 컴퓨터나 무인 정보 단말기 등의 활용에 적합하도록 하였다. 동공 후보 영역을 계산하여 검출한 후, 학습 규칙을 사용하여 얼굴에 대응하는 두 개의 지점을 선택하도록 하였다. 얼굴 검출 성능을 증명하기 위하여, 얼굴의 최대 돌출 부위를 지정 할 수 있는 이중 모드의 얼굴 추적 장치를 개발하였다. 추정치 와 복합 얼굴 검출기의 측정치를 결합하여 특징 관계를 추적함으로 처리의 안정성을 높이었다. 또한, 실시간으로 서보의 제어장치를 원격 조정하여 항상 카메라가 이미지의 중앙을 추적하도록 함으로서 얼굴의 위치를 추정할 수 있도록 하였다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2016년도 춘계학술대회 및 임시총회
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• pp.13-13
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• 2016

Insects constitute the largest and most diverse group of animals in the world. They also serve as the hosts or nutrient sources for an immense assemblage of pathogens, parasites, and predators. More than 700 fungal species from 100 genera have adopted an entomopathogenic lifestyle. Although entomopathogenic fungi were studied as only biocontrol agents against a variety of pests in various countries, it has been recently focused their additional roles in nature. They are antagonists to/against plant pathogens, endophytes, and possibly even plant growth promoting agents. The potential antimicrobial effect against fungal plant pathogens by an isolate of entomopathogenic fungi including Beauveria bassiana, Lecanicillium spp., and Isaria fumosorosea have been reported since late 1990s, but wasn't reported pathogenicity of the isolate against pests. Later, a Canadian Lecanicillium sp. isolate and L. longisporium isolated from Vertalec$^{(R)}$ showed simultaneous control effect against both aphid and cucumber powder mildew. Therefore, the antimicrobial activities of 342 fungi isolates collected from various regions and conditions in Korea were evaluated against plant pathogenic fungus Botrytis cinerea using dual culture technique on agar plate. As a result, 186 isolates (54.4%) shown the antifungal activity against B. cinerea. The culture filtrates of selected fungi completely suppressed the growth of the microorganisms, indicating that suppression was due to the presence of antimicrobial substances in the culture filtrate. Mode of action of these fungi against insect involves the attachment of conidia to the insect cuticle, followed by germination, cuticle penetration, and internal dissemination throughout the insect. During infection process, secreted enzymes, proteinous toxins, and/or secondary metabolites secreted by entomopathogenic fungi can be used to overcome the host immune system, modify host behavior, and defend host resources. Recently, secondary metabolites isolated from entomopathogenic fungi have been reported as potential bioactive substances. Generally, most of bioactive substances produced by entomopathogenic fungi have reported low molecular weight (lower than 1,000 g/mol) as peptide and, in contrast the high molecular weight fungal bioactive substances are rare. Most substances based on entomopathogenic fungi were shown antimicrobial activity with narrow control ranges. In our study we analyzed the antimicrobial substances having antagonistic effects to B. cinerea. Antimicrobial substances in our fungal culture filtrates showed high thermostability, high stability to proteolytic enzymes, and hydrophilicity and their molecular weights were differed from substance. In conclusion, entomopathogenic fungi showed pathogenicity against insect pests and culture filtrate of the fungi also shown to antimicrobial activity. In the future, we can use the entomopathogenic fungi and its secondary metabolites to control both insect pest control and plant pathogenic fungi simultaneously.

• Weed & Turfgrass Science
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• 제2권3호
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• pp.221-229
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• 2013

우리나라에서 제초제 저항성작물의 개발과 저항성작물의 실용화 재배가 이루어질 경우를 대비하기 위하여 제초제 저항성작물 개발 및 잡초방제 연구현황을 분석하고 앞으로의 잡초방제 방향과 전망에 관하여 기술하였다. 제초제 저항성작물 개발은 작물에 대한 약해 없이 한 가지 제초제만을 사용해서 선택적으로 광범한 잡초방제 효과를 나타낼 수 있는 획기적인 잡초관리 기술의 하나이다. Glyphosate 저항성 형질전환 콩, 옥수수, 캐놀라, 면화에서 glyphosate 사용은 단순한 잡초방제법으로 저비용으로 편리하게 잡초를 관리할 수 있다. 한 작물에 glyphosate와 glufosinate에 모두 저항성인 이중저항성작물은 옥수수, 콩, 면화에서 개발되어 glyphosate 저항성잡초를 방제할 수 있었으며, 기존 제초제 기술의 영역을 확장할 수 있을 것이다. 그리고 glyphosate를 ALS 저해형 제초제, 합성옥신 제초제, HPPD 저해형 제초제 혹은 ACCase 저해형 제초제를 포함한 새로운 제초제 저항성작물 개발은 제초제 저항성잡초를 효과적으로 방제하기 위하여 가까운 장래에 개발되기를 기대한다. 또한 제초제 저항성작물만으로는 잡초문제를 해결할 수 없기 때문에 앞으로 다른 작용기작 제초제의 교호사용, 기계적, 경종적 방법을 통합한 종합적잡초관리 체계에 관한 연구가 요망된다.

• 식물병연구
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• 제16권2호
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• pp.163-169
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• 2010

마늘 저장병을 일으키는 푸른곰팡이병을 생물적으로 방제하기 위하여 마늘과 양파 등 파속 채소 근권 또는 근면으로부터 미생물 1,292균주를 분리하였다. 분리한 균주를 대상으로 푸른곰팡이병에 대한 항균성 검정과 마늘인 편검정을 실시하여 최종적으로 푸른곰팡이병 진전을 현저히 억제하는 S59-4균주를 선발하였으며, Biolog system을 이용하여 동정한 결과 Pantoea agglomerans 59-4(Pa59-4)로 동정되었다. 선발한 길항균 Pa59-4의 억제기작을 조사하기 위하여 TSA배지 상에서 병원균과 대치 배양하였을 때 항균활성을 보이지 않았다. 길항균 Pa59-4과 병원균간의 영양원 경합여부를 Janisiewicz(2000)의 방법으로 조사한 결과, 저농도로 마늘즙액에 병원균과 길항균 Pa59-4를 함께 처리하였을 때, 길항균 Pa59-4는 병원균의 포자발아 및 균사생장을 현저히 억제하였으며, 길항균 Pa59-4와 함께 접종하였을 때 발아되지 않았던 병원균 포자를 신선한 5% 마늘쥬스에 접종했을 때에는 다시 잘 발아 되는 것으로 나타나 길항균 Pa59-4균주의 억제기작은 영양원 경합에 의한 것으로 확인되었다. 이상의 결과로 볼 때, 길항균 Pa59-4는 마늘저장 중 부패를 줄일 수 있는 유망한 길항균으로 판단되었다.