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http://dx.doi.org/10.5423/RPD.2010.16.2.163

Elucidation of Mode of Action of Pantoea agglomerans 59-4 for Controlling Garlic Blue Mold  

Kim, Yong-Ki (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA)
Kwon, Mi-Kyung (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA)
Yeh, Wan-Hae (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA)
Hong, Sung-Jun (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA)
Jee, Hyung-Jin (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA)
Park, Jong-Ho (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA)
Han, Eun-Jung (Department of Organic Agriculture, Division of Agro-Food Safety, NAAS, RDA)
Park, Kyung-Seok (Department of Agricultural Microbiology, Division of Agricultural Biology, NAAS, RDA)
Lee, Sang-Yeob (Department of Agricultural Microbiology, Division of Agricultural Biology, NAAS, RDA)
Lee, Seong-Don (Department of Research Coordination, Division of Research Policy Bureau, RDA)
Publication Information
Research in Plant Disease / v.16, no.2, 2010 , pp. 163-169 More about this Journal
Abstract
To screen for potential biocontrol agents against postharvest disease of garlics caused by Penicillium hirsutum, a total of 1292 isolates were isolated from the rhizoshere or rhizoplane of Allium species. Among them, S59-4 isolate was selected as a potential biocontrol agent by in vivo wounded garlic bulb assay. The isolate was identified as Pantoea agglomerans (Pa59-4) through Biolog system. Pa59-4 did not inhibit the mycelial growth of P. hirsutum in dual-culture with P. hirsutum on tryptic soy agar. In order to elucidate mode of action of Pa59-4 on biological control, nutrient competition between Pa59-4 and P. hirsutum was investigated by the simple method using tissue culture plates with cylinder inserts containing defusing membrane reported by Janisiewicz et al. (2000). The results showed that Pa59-4 effectively suppressed spore germination and mycelial growth of blue mold in the low concentration (0.5%) of garlic juice, but it did not suppress those of blue mold in the high concentration (5%) of garlic juice. This result suggests that the mechanism in biocontrol of garlic blue mold by Pa 59-4 may be involved in nutrient competition with P. hirsutum on garlic bulbs.
Keywords
Garlic blue mold; Nutrient competition; Pantoea agglomerans 59-4; Postharvest disease;
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1 Janisiewicz, W. J., Tworkoski, T. J. and Sharer, C. 2000. Characterizing the mechanism of biological control of postharvest diseases on fruits with a simple method to study competition for nutrients. Phytopathology 90: 1196-1200.   DOI
2 Kohchi, C., Inagawa, H., Nishizawa, T., Yamaguchi, T., Nagai, S. and Soma, G. I. 2006. Application of lipopolysaccharide derived from Pantoea agglomerans(IP-PA1) for health care based on macrophage network theory. J. Biosci. Bioeng. 102: 485-496.   DOI
3 Meziane, H., Gavriel, S., Ismailov, Z., Chet, I., Chernin, L. and Hfte, M. 2006. Control of green and blue mold on orange fruit by Serratia plymuthica strains IC14 and IC1270 and putative modes of action. Postharvest Biol. Biochem. 39: 125-133.   DOI
4 Nunes, C., Usall, J., Teixido, N. and Vinas, I. 2001. Biological control of postharvest pear diseases using Pantoea agglomerans CPA-2. Int. J. Food Microbiol. 70: 53-61.   DOI
5 Calvente, V., Benuzzi, D. and de Tosetti, M. I. S. 1999. Antagonistic action of siderophore from Rhdotorula glutinis upon the postharvest pathogen Penicillium expansum. Int. Biodeter. Biodegr. 43: 167-172.   DOI
6 Stockwell, V. O., Johnson, K. B., Sugar, D. and Loper, J. E. 2002. Antibiosis contributes to biological control of fire blight by Pantoea agglomerans strain Eh252 in orchards. Phytopathology 92: 1202-1209.   DOI
7 Nunes, C., Usall, J., Teixido, N., Fons, E. and Vinas, I. 2002. Postharvest biological control by Pantoea agglomerans (CPA-2) on golden delicious apples. J. Appl. Microbiol. 92: 247-255.   DOI
8 Park, K., Diby, P. and Yeh, W. H. 2006. Bacillus vallismortis EXTN-1-mediated growth promotion and disease suppression in rice. Plant Pathol. J. 22: 278-282.   과학기술학회마을   DOI
9 Poppe, L., Vanhoutte, S. and Hofte, M. 2003. Modes of action of Pantoea agglomerans CPA-2, an antagonist of postharvest pathogens on fruits. Eur. J. Plant Pathol. 109: 963-973.   DOI
10 Pusey, P. L., Stockwell, V. O. and Rudell, D. P. 2008. Antibiosis and acidification by Pantoea agglomerans strain E325 may contribute to suppression of Erwinia amylovora. Phytopathology 98: 1136-1143.   DOI
11 Tsukioka, D., Nishizawa, T., Miyase, T., Achiwa, K., Suda, T., Soma, G. I. and Mizuno, D. 1997. Structural characterization of lipid A obtained from Pantoea agglomerans lipopolysaccharide. FEMS Microbiol. Lett. 149: 239-244.   DOI
12 Wilson, C. L., Ghaouth, A., Chalutz, E. E., Droby, S., Stevens, C., Lu, Y. and Arul, J. 1994. Potential of induced resistance to control postharvest diseases of fruits. Plant Dis. 78: 837-844.   DOI
13 Wright, S. A. I., Zumoff, C. H., Schneider, L. and Beer, S. V. 2001. Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro. Appl. Environ. Microbiol. 67: 284-292.   DOI
14 Castoria, R., De Curtis, F., Lima, D. G., Caputo, L., Pacifico, S. and De Cicco, V. 2001. Aureobasidium pullulans(LS-30) an antagonist of postharvest pathogens of fruits: study on its modes of action. Postharvest Biol. Technol. 22: 7-17.   DOI
15 Feng, Y., Shen, D. and Song, W. 2006. Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthates. J. Appl. Microbiol. 100: 938-945.   DOI
16 Han, D. Y., Coplin, D. L., Bauer, W. D. and Hoitink, H. A. J. 2000. A rapid bioassay for screening rhizosphere microorganisms their ability to induce systemic resistance. Phytopathology 90: 327-332.   DOI