• Journal of Korean Society of Water and Wastewater
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• v.24 no.1
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• pp.5-14
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• 2010

A pilot-scale biological aerated filter (BAF) was operated with an anaerobic, anoxic and oxic zone at $23{\pm}1^{\circ}C$. The influent sCOD and total nitrogen concentrations in the feedwater were approximately 250 mg/L and 35 mg N/L, respectively. sCOD removal at optimum hydraulic retention time (HRT) of 3 hours with recirculation rates of 100, 200 and 300% in the column was more than 96%. Total nitrogen removal was consistently above 80% for 4 and 6 hours HRT at 300% recirculation. For 3 hours HRT and 300% recirculation, total nitrogen removal was approximately 79%. Based on fitting results, the kinetic parameter values on nitrification and denitrification show that as recirculation rates increased, the rate of ammonia and nitrate transformation increased. The ammonium loading rates for maximum ammonium removed were 0.15 and 0.19 kg $NH_3$-N/$m^3$-day for 100% and 200% recirculation, respectively. The experimental results demonstrated that the BAF can be operated at an HRT of 3 hours with 200 - 300% recirculation rates with more than 96 % removal of sCOD and ammonium, and at least 75% removal of total nitrogen.

• Korean Chemical Engineering Research
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• v.43 no.2
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• pp.272-277
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• 2005

Characteristics of bubbling behavior and bubble properties were investigated in a gas-liquid countercurrent bubble column of in diameter 0.152 m and 3.5 m in height, respectively. Effects of gas and liquid velocities and bubble distribution mode(even, wall-side, central or asymmetric distribution) on the bubble properties such as chord length, frequency, rising velocity and holdup in the reactor were measured and examined by means of dual resistivity probe method. The bubble size, frequency and holdup increased with increasing gas($U_G$) or liquid velocity($U_L$). The rising velocity of bubbles increased with increasing $U_G$, whereas decreased with increasing $U_L$. The uniformity of bubble size distribution and bubble holdup decreased when the distribution mode of bubbles at the gas distributor was changed from even to wall-side, central or asymmetric. The central distribution of bubbles was better than asymmetric mode but worse than wall-side distribution, in considering the bubble holdup and uniformity of distribution.

• Korean Journal of Food Science and Technology
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• v.24 no.2
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• pp.171-176
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• 1992

Volatile flavor components of Codonopsis lanceolata were extracted by gas co-distillation (GCD), solvent extraction/fractionation (SEF), and headspace sampling (HSS) methods. The extracts were analyzed by dual-capillary gas chromatography-retention index (GC-RI) and gas chromatography-mass spectrometry(GC-MS). The two extracts prepared by SEF and HSS gave more similar fragrance to the Codonopsis lanceolata than the GCD extract. The GC profiles of the SEF and HSS extracts were similar to each other except for differences in peak areas. The extract prepared by SEF gave a sweet note while the extract prepared by HSS gave a green note. The GCD extract began to give a burnt note of herb medicine with prolonged distillation. Rapid extraction of flavor components from Codonopsis lanceolata was possible in several short steps by SEF and HSS methods compared to GCD. GC-MS and GC-RI were used for peak identification. GC-RI was more effective for identification of isomers, and polar FFAP column was more suitable for identification of polar compounds. From Codonopsis lanceolata we identified 35 volatile flavor constituents, 24 of which have not been previously reported by simultaneous distillation extraction method $^{(5)}$. trans-2-Hexanal, cis-3-hexen-1-ol, trans-2-hexen-1-ol, and hexanol were considered key components of the green note and 1-octen-3-ol, the component of the fresh note. Esters, including amyl propionate, seem to be responsible for the sweet note particular to Codonopsis lanceolata.

• Korean Chemical Engineering Research
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• v.49 no.5
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• pp.582-587
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• 2011

Holdup characteristics of bubble, wake and continuous liquid phases were investigated in bubble columns with viscous liquid media. Effects of column diameter(0.051, 0.076, 0.102 and 0.152 m ID), gas velocity($U_G$=0.02~0.16 m/s) and liquid viscosity(${\mu}_L$=0.001~0.050 $Pa{\cdot}s$) of continuous liquid media on the holdups of bubble, wake and continuous liquid phases were discussed. The three phase such as bubble, wake and continuous liquid phases were classified successfully by adapting the dual electrical resistivity probe method. Compressed filtered air and water or aqueous solutions of CMC(Carboxy Methyl Cellulose) were used as a gas and a liquid phase, respectively. To detect the wake as well as bubble phases in the bubble column continuously, a data acquisition system(DT 2805 Lab Card) with personal computer was used. The analog signals obtained from the probe circuit were processed to produce the digital data, from which the wake phase was detected behind the multi-bubbles as well as single bubbles rising in the bubble columns. The holdup of bubble and wake phases decreased but that of continuous liquid media increased, with an increase in the column diameter or liquid viscosity. However, the holdup of bubble and wake phases increased but that of continuous media decreased with an increase in the gas velocity. The holdup ratio of wake to wake to bubble phase decreased with an increase in the column diameter or gas velocity, however, increased with an increase in the viscosity of con-tinuous liquid media. The holdups of bubble, wake and continuous liquid media could be correlated in terms of operating variables within this experimental conditions as: ${\varepsilon}_B=0.043D^{-0.18}U_G^{0.56}{\mu}_L^{-0.13}$, ${\varepsilon}_W=0.003D^{-0.85}U_G^{0.46}{\mu}_L^{-0.10}$, ${\varepsilon}_C=1.179D^{0.09}U_G^{-0.13}{\mu}_L^{0.04}$.

• Korean Journal of Food Science and Technology
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• v.34 no.3
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• pp.505-509
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• 2002

In the course of screening program for VHR DS-PTPase (dual-specificity protein tyrosine phosphatase) from natural sources, Gastrodia elata was selected. One compound showing potent inhibitory activity was isolated by the solvent extraction and column chromatography including silica gel, ODS RP-18, Sephades LH-20, and HPLC. This compound was identified as baicalein by several NMR techniques such as $^1H-NMR$, $^{13}C-NMR$, and DEPT. Baicalein showed selective inhibitory activity against VHR DS-PTPase with $IC_{50}=2.4\;{\mu}M$, and showed cytotoxicity against several human cancer cell lines with an $GI_{50}$ of $5.26{\sim}12.93\;{\mu}g/mL$ range, including, melanoma (LOX-IMVI), lung cancer (NCI H23 and A549), colon cancer (HCT 116 and SW 620), prostate cancer (PC-3), and leukemia (MOLT 4F).

• Korean Journal of Food Science and Technology
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• v.26 no.6
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• pp.665-669
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• 1994

A rapid gas chromatographic (GC) profiling method was applied to dairy products (milk and cheeses) for the simultaneous analysis of volatile and nonvolatile organic acids. Cheese samples were first made into aqueous samples by dissolving in water. The aqueous samples were then extracted with organic solvents after the acidification and NaCI saturation. The organic layers (diethyl ether : hexane= 1 : 1) were extracted with $NaHCO_3$ saturated solution with subsequent solid-phase extraction of the aqueous phases using Chromosorb P column/diethyl ether followed by triethylamine treatment. The resulting triethylammonium salts of acids were directly converted into volatile tert.-butyldimethylsilyl derivatives, which were analyzed by dual-capillary column GC, and GC-mass spectrometry. From milk and four cheese samples studied, 31 organic acids including 21 fatty acids and other hydroxy and dioic acids were tentatively identified. The amounts of the fatty acids were different among the kinds of cheese and thus the simplified retention index (RI) spectra of organic acids were useful for the visual pattern recognition of each sample, when the Direct Comparision method between cheese and a blind sample were attempted, it was quickly recognized to be a gouda cheese with the 999 ppt match quality value.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.552-559
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• 2008

Ethyl (S)-4-chloro-3-hydroxybutyrate is an intermediate for the synthesis of Atorvastatin, a chiral drug used for hypercholesterolemia. A Rhodococcus erythropolisstrain (No.7) able to convert 4-chloro-3-hydroxybutyronitrile into 4-chloro-3-hydroxybutyric acid has recently been isolated from soil. This activity has been regarded as having been caused by the successive actions of the nitrile hydratase and amidase. In this instance, the corresponding amidase gene was cloned from the R. erythropolis strain and expressed in Escherichia coli cells. A soluble active form of amidase enzyme was obtained at $18^{\circ}C$. The Ni column-purified recombinant amidase was found to have a specific activity of 3.89 U/mg toward the substrate isobutyramide. The amidase was found to exhibit a higher degree of activity when used with mid-chain substrates than with short-chain ones. Put differently, amongst the various amides tested, isobutyramide and butyramide were found to be hydrolyzed the most rapidly. In addition to amidase activity, the enzyme was found to exhibit acyltransferase activity when hydroxyl amine was present. This dual activity has also been observed in other enzymes belonging to the same amidase group (E.C. 3.5.1.4). Moreover, the purified enzyme was proven to be able to enantioselectively hydrolyze 4-chloro-3-hydroxybutyramide into the corresponding acid. The e.e. value was measured to be 52% when the conversion yield was 57%. Although this e.e. value is low for direct commercial use, molecular evolution could eventually result in this amidase being used as a biocatalyst for the production of ethyl (S)-4-chloro-3-hydroxybutyrate.

• Journal of the Ergonomics Society of Korea
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• v.31 no.2
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• pp.379-388
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• 2012

Objective: This study developed a new shower carrier prototype to reduce caregivers' muscle burden and to increase use convenience by reflecting the needs of domestic long-term care institutions. Background: In the long-term care institutions, one of the ADL(Activities of Daily Life) factors is bathing/showering. Recently, bath/shower-assisting equipment is actively being introduced in care institutions to reduce the caregivers' care cost, but most of the domestic equipment was designed to imitate foreign products and rarely reflected the needs of care institutions. Method: Based on Korean elderly people's body information, the bed size(length: 1,900mm, width: 650mm) was set-up, and a variable headrest with a newly designed headform was developed to provide the comfort for the elderly and convenience for caregivers. To reduce caregivers' muscle burden on transferring and showering activities, a 3-step column lifting module equipped with dual actuators(lowest/highest levels from the ground: 600/1,100mm, Stroke: 500mm) was developed, and the wheelbase parameter(length: 1,250mm, width: 580mm) was defined securing the turn-over safety of the shower carrier. The drivability tests were performed for the prototype and foreign product, and the male and female subject's muscle activities were measured through the tests. Results: The structural stability of the shower carrier prototype was secured by finite element analysis, and the muscle activities of the subjects through the drivability tests largely decreased in the prototype, compared to the foreign product. Conclusion: In this study, a new shower carrier prototype was developed to possibly reduce caregivers' muscle burden and to increase use convenience based on the needs of long-term care institutions. It was expected that the drivability performance of the prototype could be relatively superior to that of the foreign product. Application: The results obtained from the study can be applied for the optimal development of a shower carrier including other equipment to effectively care for the elderly.

• The Korean Journal of Food And Nutrition
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• v.28 no.5
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• pp.858-865
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• 2015

The study was carried out to investigate the efficacy of current nutrition labeling of processed foods and examine awareness among university students of nutrition information given current nutrition labels, and also awareness of the one-serving size of snacks that come in differently sized packages. A total of 100 students in Chungbuk, Korea participated in this study. About 60% of the subjects knew about nutrition labeling; however, a very small percentage (11%) of the subjects reported an experience of being educated about nutrition labeling. Also, just 32% of the students checked nutrition labels when they purchased processed foods. When understanding of nutrition information on labels of snacks in differently sized packaging was tested, over 80% of the subjects understood nutrition information accurately. Five different package sizes were studied. Of these 5, packages containing about 200% of one standard serving showed the highest rate of misunderstanding of total energy contents per container. Interestingly, the students most strongly preferred containers that contained 300% of one standard serving. From these results, we conclude that students consider the current recommendation for a serving size to be small, which may be related to misreading of nutrition labels of snacks that come in packages containing less than 300% of one standard serving size. To improve accurate understanding of nutrition labels of snacks, total package labels or dual column labels on packages containing less than 300% of one standard serving size should be considered.

• Journal of the Earthquake Engineering Society of Korea
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• v.13 no.2
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• pp.59-68
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• 2009

For safe and economical design to provide strong earthquake resistance, the moment redistribution and plastic rotation of structures and their members needs to be evaluated. To achieve this, an earthquake design method was developed using secant stiffness analysis. To address the variation of member stiffness due to plastic rotation and moment redistribution, a structure was modeled with a beam-column element with non-rigid end connections (NREC element). Secant stiffness for the NREC element was determined based on the ductility demands of the structure and members. By performing a conventional linear analysis for the secant stiffness model, redistributed moments and plastic rotations of the members were computed. The proposed method was applied to a moment frame and two dual systems. The design results were verified using detailed nonlinear analyses.