• Journal of Genetic Medicine
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• 제5권1호
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• pp.15-20
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• 2008

목 적 : 듀시엔/베커형 근이영양증(Duchenne and Becker type muscular dystrophy; DMD/BMD)은 남아에게 나타나는 일반적인 X 염색체 연관 유전성 근육 질환으로 DMD(dystrophin) 유전자의 돌연변이로 인해 생긴다. 그 중 큰 exon 결실이 전체 DMD 환자의 약 50-60%에서 발견된다. 이 유전자의 돌연변이를 찾기 위해 여러 방법들이 사용되고 있지만 결실 돌연변이를 찾아내기 위한 가장 일반적인 방법으로 복합적 중합효소연쇄반응을 이용하고 있다. 하지만 이 방법의 단점은 하나의 시험관 안에 복합적인 시발체가 존재하여 정확한 반응 조건을 찾기 힘들 뿐 아니라 시발체 상호간의 간섭으로 중합효소 연쇄반응의 비특이적 생성물을 빈번하게 일으켜 잘못된 음성 또는 양성 결과를 가져올 수 있다. 이런 문제를 보완하고자 Dual Primer Oligonucleotide(DPO) 방법을 도입하였다. DPO는 polydeoxyinosine 연결에 의해 두 영역으로 분리된 올리고뉴클레오티드(oligonucleotide)로 표적 DNA 염기서열과의 교잡반응에 높은 특이적 반응을 보여 복합 중합효소 연쇄반응의 정확도를 높여준다. 본 연구에서는 3 그룹을 대상군으로 DMD 유전자의 결실돌연변이 검색을 위한 DPO-복합 중합효소 연쇄반응법의 특이성과 민감성을 알아보고자 하였다. 방 법 : 50명의 건강한 남자 대조군, 50명의 결실 돌연변이를 갖고 있는 양성반응 환자그룹 그리고 20명의 결실 돌연변이를 가지고 있지 않은 음성반응 환자 그룹으로 구성된 3 그룹을 대상으로 DPO-복합 중합효소 연쇄반응법을 이용하여 실험하였다. 이들 120명의 실험군 모두 PMter영역과 exon 3, 4, 6, 8, 12, 13, 17, 19, 43-48, 50-52, 60을 포함하는 18개의 exon에서의 결실의 여부와 결실 범위를 확인하였다. 결 과 : DPO-복합 중합효소 연쇄반응법은 결실 여부를 발견하는데 100%의 특이성과 민감성을 보였다. 하지만 결실 범위의 결정에는 97.1%의 민감성과 특이성을 보였다. 결 론 : DPO-복합 중합효소 연쇄반응법은 기존의 복합 중합효소 연쇄반응법 보다 높은 분석 확실성을 보일뿐 아니라 빠르고 저렴한 비용으로 쉽게 할 수 있기 때문에 듀시엔/베커형 근이영양증 환자의 DMD 유전자의 결실돌연변이 여부를 확인하는데 유용한 방법이다.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2010년도 하계학술대회
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• pp.150-151
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• 2010

본 논문에서는 잡음환경에서의 이중채널 음성인식을 위한 통계모델 기반 음성구간 검출 방법을 제안한다. 제안된 방법에서는 다채널 입력 신호로부터 얻어진 공간정보를 이용하여 음성 존재 및 부재 확률모델을 구하고 이를 통해 음성구간 검출을 행한다. 이때, 공간정보는 두 채널간의 상호 시간 차이와 상호 크기 차이로, 음성 존재 및 부재 확률은 가우시안 커널 밀도 기반의 확률모델로 표현된다. 그리고 음성구간은 각 시간 프레임 별 음성 존재 확률 대비 음성 부재 확률의 비를 추정하여 검출된다. 제안된 음성구간 검출 방법의 평가를 위해 검출된 구간만을 입력으로 하는 음성인식 성능을 측정한다. 실험결과, 제안된 공간정보를 이용하는 통계모델 기반의 음성구간 검출 방법이 주파수 에너지를 이용하는 통계모델 기반의 음성구간 검출 방법과 주파수 스펙트럼 밀도 기반 음성구간 검출 방법에 비해 각각 15.6%, 15.4%의 상대적 오인식률 개선을 보였다.

• Journal of Electrical Engineering and Technology
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• 제10권4호
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• pp.1915-1920
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• 2015

ISO 26262 is an international standard for the functional safety of electric and electronic systems in vehicles, and this standard has become a major issue in the automotive industry. In this paper, a functional safety compliant electronic control unit (ECU) for an electric power steering (EPS) system and a demonstration purposed EPS system are developed, and a software and hardware structure for a safety critical system is presented. EPS is the most recently introduced power steering technology for vehicles, and it can improve driver’s convenience and fuel efficiency. In conformity with the design process specified in ISO 26262, the Automotive Safety Integrity Level (ASIL) of an EPS system is evaluated, and hardware and software are designed based on an asymmetric dual processing unit architecture and an external watchdog. The developed EPS system effectively demonstrates the fault detection and diagnostic functions of a functional safety compliant ECU as well as the basic EPS functions.

• Toxicological Research
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• 제17권
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• pp.59-69
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• 2001

Arsenic exposure is associated with several human diseases, including cancers, atherosclerosis, hypertension, and cerebrovascular diseases. In cultured cells, arsenite, an inorganic arsenic com-pound, was demonstrated to interfere with many physiological functions, such as enhancement of oxidative stress, delay of cell cycle progression, and induction of structural and numerical changes of chromosomes. The objective of this study is to investigate the effects of arsenic exposure on gene expression profiles by colorimetric cDNA microarray technique. HFW (normal human diploid skin fibroblasts), CL3 (human lung adenocarcinoma cell line), and HaCaT (immortalized human keratinocyte cell line) were treated with 5 $\mu\textrm{M}$ or 10 $\mu\textrm{M}$ sodium arsenite for 6 or 16 h, respectively. By a dual-color detection system, the expression profile of arsenite-treated cultures was compared to that of control cultures. Several genes expressed differentially were identified on the microarray membranes. For example, MDM2, SWI/SNF, ubiquitin specific protease 4, MAP3K11, RecQ protein-like 5, and Ribosomal protein Ll0a were consistently induced in all three cell types by arsenite, whereas prohibitin, cyclin D1, nucleolar protein 1, PCNA, Nm23, and immediate early protein (ETR101) were apparently inhibited. The present results suggest that arsenite insults altered the expression of several genes participating in cellular responses to DNA damage, stress, transcription, and cell cycle arrest.

• Fisheries and Aquatic Sciences
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• 제14권3호
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• pp.218-225
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• 2011

Dual frequency identification sonar (DIDSON) is an imaging sonar that has been used for numerous fisheries investigations in a diverse range of freshwater and marine environments. The main purpose of DIDSON is fish counting, fish sizing, and fish behavioral studies. DIDSON records video-quality data, so processing power for handling the vast amount of data with high speed is a priority. Therefore, a semiautomated analysis of DIDSON data for fish counting, sizing, and fish behavior in Echoview (fisheries acoustic data analysis software) was accomplished using testing data collected on the Rakaia River, New Zealand. Using this data, the methods and algorithms for background noise subtraction, image smoothing, target (fish) detection, and conversion to single targets were precisely illustrated. Verification by visualization identified the resulting targets. As a result, not only fish counts but also fish sizing information such as length, thickness, perimeter, compactness, and orientation were obtained. The alpha-beta fish tracking algorithm was employed to extract the speed, change in depth, and the distributed depth relating to fish behavior. Tail-beat pattern was depicted using the maximum intensity of all beams. This methodology can be used as a template and applied to data from BlueView two-dimensional imaging sonar.

• 통합자연과학논문집
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• 제3권3호
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• pp.168-173
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• 2010

Novel porous silicon chip exhibiting dual optical properties, both Frbry-Perot fringe (optical reflectivity) and photoluminescence had been developed and used as chemical sensors. Porous silicon samples were prepared by an electrochemical etch of p-type sillicon wafer (boron-doped, <100> orientation, resistivity 1 - 10 ${\Omega}$). The ething solution was prepared by adding an equal volume of pure ethanol to an aqueous solution of HF (48% by weight). The porous silicon was illuminated with a 300 W tungsten lamp for the duration of etch. Ething was carried out as a two-electrode Kithley 2420 preocedure at an anodic current. The surface of porous silicon was characterized by FT-IR instrument. The porosity of samples was about 80%. Three different types of porous silicon, fresh porous silicon (Si-H termianated), oxidized porous silicon (Si-OH terminated), and surface-derivatized porous silicon (Si-R terminated), were prepared by the thermal oxidation and hydrosilylation. Then the samples were exposed to the wapor of various organics vapors. such as chloroform, hexane, methanol, benzene, isopropanol, and toluene. Both reflectivity and photoluminescence were simultaneously measured under the exposure of organic wapors.

• 한국음향학회지
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• 제20권3호
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• pp.42-47
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• 2001

본 논문에서는 인터넷 폰 및 화상통신 등을 위해 개발된 G.723.1 부록 A 6.3kbps/5.3kbps 이중 전송율 음성 코덱의 음성활동 검출기의 성능을 개선한다. 본 논문에서는 에너지 레벨을 크게 3가지 범위로 구분하여 음성활동 유무 판정을 하도록 한다. 제안한 알고리즘에 대한 처리시간, 음질평가 및 전송율 감소량을 측정한 결과 처리시간의 경우 묵음구간에 대한 판정의 정확성에 기인하여 G.723.1에 비해 감소하고 주관적인 음질평가의 경우 G.723.1과 거의 차이가 없다. 전송율 측정을 위해 VAD=1로 판정한 프레임을 측정한 결과 묵음 구간이 많이 나타날수록 뚜렷한 전송율 감소효과를 얻을 수 있다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2008년도 International Symposium on Remote Sensing
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• pp.188-191
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• 2008

The Tonle Sap, Cambodia, is a huge lake and periodically flooded due to monsoon climate. The incoming water causes intensive flooding that expands the lake over vast floodplain and wetland consisting mainly of forests and shrubs. Monitoring the water-level change over the floodplain is essential for flood prediction and water resource management. A main objective of this study is flood monitoring over Tonle Sap area using ALOS PALSAR. To study double-bounce effects in the lake, backscattering effect using ALOS PALSAR dual-polarization (HH, HV) data was examined. InSAR technique was applied for detection of water-level change. HH-polarization interferometric pairs between wet and dry seasons were best to measure water level change around northwestern parts of Tonle Sap. The seasonal pattern of water-level variations in Tonle Sap studied by InSAR method is similar to the past and altimeter data. However, water level variation measured by SAR was much smaller than that by altimeter because the DInSAR measurement only represents water level change at a given region of floodplain while altimeter provides water level variation at the central parts of the lake.

• 한국초전도ㆍ저온공학회논문지
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• 제6권2호
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• pp.25-28
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• 2004

Three-phase inductive superconducting fault current limiter (SFCL) with DC reactor rated on 6.6 $KV_{rms}/200 A_{rms}$ has been developed in Korea. This system consists of one DC reactor, AC/DC power converter, and a three-phase transformer, which is called magnetic core reactor (MCR). This paper deals with the short-circuit analysis of the SFCL. The DC reactor was the HTS solenoid coil whose inductance was 84mH. The power converter was performed as the dual-mode operation for dividing voltage between the rectifying devices. The short-term normal operation (1 see) and short-circuit tests (2∼3 cycles) of this SFCL were performed successfully. In regular short-circuit test, the fault current was limited as 30% of rated short-circuit current at 2 cycles after the fault. The experimental results have a very similar tendency to the simulation results. Using the technique for the fault detection and SCR firing control, the fault current limiting rate of the SFCL was improved. From this research, the parameters for design and manufacture of large-scale SFCL were obtained.

• BMB Reports
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• 제46권12호
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• pp.600-605
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• 2013

Interplay between calcium ions ($Ca^{2+}$) and reactive oxygen species (ROS) delicately controls diverse pathophysiological functions of vascular smooth muscle cells (VSMCs). However, details of the $Ca^{2+}$ and ROS signaling network have been hindered by the absence of a method for dual measurement of $Ca^{2+}$ and ROS. Here, a real-time monitoring system for $Ca^{2+}$ and ROS was established using a genetically encoded hydrogen peroxide indicator, HyPer, and a ratiometric $Ca^{2+}$ indicator, fura-2. For the simultaneous detection of fura-2 and HyPer signals, 540 nm emission filter and 500 nm~ dichroic beamsplitter were combined with conventional exciters. The wide excitation spectrum of HyPer resulted in marginal cross-contamination with fura-2 signal. However, physiological $Ca^{2+}$ transient and hydrogen peroxide were practically measurable in HyPer-expressing, fura-2-loaded VSMCs. Indeed, distinct $Ca^{2+}$ and ROS signals could be successfully detected in serotonin-stimulated VSMCs. The system established in this study is applicable to studies of crosstalk between $Ca^{2+}$ and ROS.