• Title/Summary/Keyword: dual culture

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Identification of an Antifungal Chitinase from a Potential Biocontrol Agent, Bacillus cereus 28-9

  • Huang, Chien-Jui;Wang, Tang-Kai;Chung, Shu-Chun;Chen, Chao-Ying
    • BMB Reports
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    • v.38 no.1
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    • pp.82-88
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    • 2005
  • Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5$\alpha$. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.

Mycoparasitism of Acremonium strictum BCP on Botrytis cinerea, the Gray Mold Pathogen

  • Choi, Gyung-Ja;Kim, Jin-Cheol;Jang, Kyoung-Soo;Cho, Kwang-Yun;Kim, Heung-Tae
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.167-170
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    • 2008
  • A fungal strain BCP, which parasitizes Botrytis cinerea gray mold pathogen, was isolated and identified as Acremonium strictum. BCP strain overgrew the colonies of B. cinerea and caused severe lysis of the host hyphae. Frequent penetration and hyphal growth of A. strictum BCP inside the mycelia of B. cinerea were observed under light microscopy. In addition, some morphological abnormalities such as granulation and vacuolation of the cytoplasm were observed in mycelia and spores of B. cinerea. In dual culture test, A. strictum BCP strongly inhibited the mycelial growth of several plant pathogenic fungi as well as B. cinerea. To our knowledge, this is the first report on mycoparasitism of Acremonium species on B. cinerea.

Antagonism of Pseudomonas spp. against to Rhizoctonia solani and Pythium spp. (Pseudomonas spp.의 Rhizoctonia solani 및 Pythium spp. 병원균에 대한 길항작용)

  • 주영규;한정훈
    • Asian Journal of Turfgrass Science
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    • v.8 no.1
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    • pp.47-52
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    • 1994
  • Attempts were made to investigate the antagonistic activity of soil borne microorganisms Pseudomonas spp. and Trichoderma spp. against to the pathogens of turf diseases Rhizoctionia solani spp. and Pythiom spp. in vitro by a dual culture bioassay. Inhibition zone between the edge of the my-celium and the margin of each antagonistic bacteria, Pocudontonas, on potato dextrose agar was measured 3 days after incubation at 28˚C. Psudomonas spp. showed relatively high inhibition of mycelium growth of R. solani AG-i and Pythium spp. which cause brown patch and pythium blight, respectively. Antagonistic fungi Trichodenma spp. also showed effective inhibition against mycelium growth of both pathogens, more proper methods of measuring the inhibition effects were required because of fast growth of Trichodenna hypae. Brown patch and pythium blight both, re-quire most higher rate of fungicide use to control in golf curses in Korea. Application of antagon-istic microorganisms are useful as biological resources an approach to sole environmental contamination.

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Belle Epoque and Dadaism in the Modern Culture (벨 에포크와 다다이즘 - 근대문화의 총체와 해체)

  • Lee, Byung Soo
    • Cross-Cultural Studies
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    • v.33
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    • pp.171-192
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    • 2013
  • The article is a research about the Belle Epoque era and Dadaism in the modern culture as a whole and separate. The years from 1890s to 1914, is known as the Belle Epoque era, in which the European continent including France had developed the climax of the modern culture after the Renaissance. At the same time, it was the period where the postmodern developments were being spread, leading to the present days. Moreover, the main ideologies in art that led to the cultural advancement of the time were impressionism, cubism, art nouveau, evolutionized painting category, symbolism and futurism. It was a literature category that was maintained to present Dadaism and surrealism. Dadaism began since the magazine, Bulletin Dada was published, originating in 1916 by Tristan Tzara of Zurich, Switzerland during the WWI. The extreme motto that the Dadaists supported was a contradiction, as they had to dissolve from their own art movements and expression techniques. However, until Andre Breton introduced 'Manifeste du Surrealisme' in 1924, the "Dada group" had a tremendous influence in France as an epicenter and rejected the modern cause and art that continued during the time, thus attempting its dissolution. First, they rejected the ideology, ethics and customs of rationalism from the previous system and demonstrate an anarchical and anti-bourgeoisie characteristic. They also reject the French lucid thoughts and the artistic techniques. They strongly emphasized on their motto "The idea is created from the mouth", while reframing from the philosophical ideology and at the same time, attempting to express the psychical unconsciousness. Second, the most important catchphrase that the Dadaists supported was the theory of negation. The question "Why do you write?" connotes the negative consciousness about the artistic value and the stereotyped method of the preexisting writing and drawing. Third, the Dadaists bring forward a radical query about all of the former esthetic and morals, and reveal an admirable resistance spirit. They emphasized on the slogan "Dada, means nothing" and insist on 'the anti-literal Dada, anti-artistic Dada, anti-musical Dada'. The Dadaist movement manifested their resistant spirit and the new artistic spirit through the publication of , , and most importantly through the magazine . Fourth, the Dadaists embodied the volume, density, and quality into an image through the auto-technical, cubistic writings and drawings. They ignored the fixed form of arrangements, verses, and rhymes of a poetic diction. The Dadaists utilized an unfamiliar and inversed expression method of applying the combination of the size of print, or capital letters and lowercase letters, even combining printed and handwritten writings. As presented, the auto-technical and cubistic characteristic of expressing the auto-psychical ideology into writing is called as the radical aesthetic and moral and can be considered as the most essential cause of the Dadaists' avant-garde features. As a conclusion, Dadaism demonstrated dual characteristics of consuming the nutritive elements of the modern culture through the most powerful resistance and liberation of the artistic movement of the Belle Epoque era, where at the same time, it deconstructed the modern art. By revolting against the former grounds and expression techniques, and dominating the era with the new artistic spirit, their resistant actions were artistic movements that symbolized the dissolution of the modern times. Moreover, the Dada's expressionism and resistance of saying "There's nothing" can be evaluated as postmodernity's initiative of outweighing the modern history and opening the door for new period of nowadays.

A Study on the lives of female politicians of Cheju island (제주도 여성정치인의 가정생활에 관한 연구)

  • Kim Myung-Sil;Kim Hye-Yeon
    • Journal of Family Resource Management and Policy Review
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    • v.9 no.3
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    • pp.45-68
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    • 2005
  • The political activity of women is relatively low. This is mainly because of the social-cultural attitude that confines women's role to housewives, rather than the structural problems that are related to political activities itself. This study has attempted to enhance the political activities of women by examining and providing the practical informations related to the hardships that are experienced by women who are pursuing a career both as a politician as well as a housewife. The sample of this study consisted of women who are assuming chief executives positions in organizations that are related to political activity, including local representatives of the national assembly. A case study was conducted by interviewing 8 female politicians. The contents of the study mostly consists of the personal situations (related to house lives) of these women. Semi-structured questionnaires were used, and the interview was conducted several times. First, the political careers of these women tended to start passively, rather than voluntarily. Second, the negative attitudes that were initially displayed by the family members and close relatives had changed positively through out the course of time. Close relatives, parents/parents-in-law were likely to become helpers of child care and household labor. The husband was likely to be a supporter of the wife's political career, and the children started to understand the roles of their mother as they grew up. Third, the female politicians perceived that the main impediments of women pursuing political careers were the sexually discriminating culture, rather than their own ability. Fourth, the fact that these women had to spend a lot of time outside the house has worsened the role conflict and difficulties of these women who are required to assume dual-roles. Furthermore, the difficulties that are faced publicly and privately has increased the psychological burdens of these women, since politicians, in general, are people who need to maintain their public image.

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Economic well-being and life satisfaction by the comparison of couple's income (부부의 소득수준 비교에 따른 가계경제복지 및 삶 만족)

  • Huh, Kyung-Ok
    • Korean Journal of Human Ecology
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    • v.13 no.3
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    • pp.441-451
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    • 2004
  • This study categorized the households into four by the comparison of wives' and husbands' earned income, such as one with higher wives' income than husbands' in dual-earner households, with higher wives' income than husbands' in household with unemployed husbands, with lower wives' income than husbands' in dual-earner households, and with lower wives' income than husbands' in household with unemployed wives. This study compared and analyzed the objective and subjective economic well-being, and overall satisfaction of life in those households. Major results are below. First, the amount of expenditures, saving, debt were low in one-earner household with employed husbands, but average propensity to expenditure was high in those households. The amount of saving was low in one-earner households with unemployed husbands, but the average propensity to expenditure and debt were high, resulting in the low status of economic well-being. The amount of saving and monetary assets were high, but average propensity to expenditure and debt were low in households in which wives had higher income than husbands, resulting in the high status of economic well-being. Second, husbands' satisfaction for the level of family income was not different among four groups of households. Wives's satisfaction for those was the lowest when wives' income was higher than husbands', but the highest in households with unemployed wives. Wives' and husbands' expectations for the future economic well-being of the households were negative in households with unemployed husbands. On the other hand, the level of satisfaction of wives and husbands for their life was not different among four groups of households. Finally, four groups of households were discriminated by age of husbands, number of children, debt, and wives' expectation for the future economic well-being.

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An ethnographic research study on experience of identity in Korean multigravidas (경임부의 정체감 경험)

  • Kim, Young-Hee
    • Korean Parent-Child Health Journal
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    • v.4 no.1
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    • pp.19-34
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    • 2001
  • The childbearing process is not only a biological phenomenon of a woman who gives birth to a child but also a sociocultural phenomenon which is reflected on her value, belief in the sociocultural context according to social change and acculturation. The familial relation and sociocultural context in the multigravidas are more complex and intermingled than in the primigravidas. The purpose of this ethnographic research study was to explore the experience of identity from the first trimester of pregnancy to the third trimester of pregnancy in the Korean multigravidas and to understand deeply the perspectives of pregnant women reflected on Korean sociocultural values, beliefs, norms and familial culture. The participants of 10 pregnant women in Seoul, Korea were observed for 10 months from January to October 2000 and interviewed in their homes and comfortable place. Data analysis was accomplished 'line by line method' and significant concepts were classified according to themes, categories, and domains. The results of this study were as follows : The participants experienced 4 categorized subjects : understanding the oneself - mother to be, performing the dual role, drifting the emotion, and living disheartened during pregnancy. The participants were showed universality and diversity pattern in the self understanding process. The universal pattern were 'mother to be' showing maturation, life along family and priority on motherhood between being a mother and a woman. The diverse pattern were taking the dual role in working mothers having the higher self actualized value and personal identity rather than maternal identity, drifting emotion in resigned mothers, and living disheartened in mothers who have two daughters and no son. In conclusion, the Korean multigravidas experienced womanhood as well as motherhood through the self understanding process with familial connections during pregnancy. Therefore it is suggested that if the harmony and the balance between a mother and a woman is accomplished, the woman will lead a healthy and high quality of life. Also, this study sought to confirm the sociocultural factors affecting during pregnancy in the perspectives of the women with children. Therefore, the health care providers have to divert their attention from biomedical perspectives to biocultural perspectives integrating bio-psycho-sociocultural aspects of pregnant women in a clinical setting.

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Decolorization of Blue-Stain by Dual Culture of Blue Staining and Basidial Fungi

  • Pashenova, Natalia;Lee, Jong-Kyu;Cho, Nam-Seok
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.2 s.130
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    • pp.65-71
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    • 2005
  • This study was performed to understand the interaction between Ophiostomataceae and basidiomycetes fungi during cultures, and whether the basidiomycetes fungi inhibit the growth and decolorize dark pigments of blue staining fungi. The conjoint cultivation was studied on 2% malt extract agar. The ability of basidial cultures to decolorize dark pigments of ophiostomatoid fungi was the main characteristics estimated during this study. More than half of basidial cultures were characterized by deadlock interaction with blue staining fungi. In the dual cultures, where basidial partners were presented by Agaricus bisporus(64), Laetiporus sulphureus(L01/89), Trametes versicolor(09) and unknown fungus(02), antagonism was found at the phase of primary contact of colonies. Replacement interaction resulted usually in decreasing dark colour of substrate was observed for 11 basidial cultures that were belonging mainly to white-rot fungi. Among them Abortiporus biennis(123), Antrodiella hoehnelii(S28/91), Bjerkandera fumosa (137), and Gleophyllum odoratum(124) were characterized by the absence of deadlock-phase: they began to grow over dark colonies of their partners just after primary contact. Basidiomycetes did not affect strongly the pigments of Ceratocystis spp. and Leptographium sibirica isolates, but completely decolorized colonies of Ophiostoma ips and to a smaller degree Ophiostoma minus. Antrodiella hoehnelii(S28/91), Bjerkandera fumosa(137), Gleophyllum odoratum(124) and Trametes versicolor(B18/91) cultures were found to be the most active in decreasing dark color of blue staining fungi colonies. The cultures were recommended for further development as agents of biopulping of wood chips and bio-control of blue stain in woods.

Analysis of the Dual Promoters and the $H_2O$$_2$-responsive Element of the cats Gene Encoding Catalase A in Streptomyces coelicolor

  • Cho, You-Hee;Hahn, Ji-Sook;Roe, Jung-Hye
    • Journal of Microbiology
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    • v.38 no.4
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    • pp.239-244
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    • 2000
  • The cats gene encodes the major catalase in Sreptomyces coelicolor, whose production increases upon H$_2$O$_2$treatment. Besides the previously identified primary promoter (catApl), a minor promoter (catAp2) was newly assigned by S1 nuclease mapping. The catAp2 transcript was observed transiently upon entry into the stationary phase in liquid culture and upon differentiation on solid plates, whereas the level of catApl transcription did not chance significantly during this growth transition. ThecatApl promoter was transcribed by the major vegetative RNA polymerase holoenzyme containing $\sigma$$\^$HrdB/, whereas the catAp2 was transcribed in vitro by the holoenzyme containing $\sigma$$\^$R/ that is activated under oxidative conditions. The cia-element regulating the H$_2$O$_2$-inducibility of catApl was identified within the 23 bp inverted repeat sequence located between -65 and -43 of the catApl promoter. We roamed this sequence HRE (H$_2$O$_2$-responsive Element). The distal half of the inverted repeat was more crucial for H$_2$O$_2$-dependent induction of the catApl transcript than the proximal half. HRE most likely serves as a binding site for the H$_2$O$_2$-responsive repressor CatR.

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An Economic Modeling Study of Helicobacter pylori Eradication: Comparison of Dual Priming Oligonucleotide-Based Multiplex Polymerase Chain Reaction and Empirical Treatment

  • Gweon, Tae-Geun;Kim, Joon Sung;Kim, Byung-Wook
    • Gut and Liver
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    • v.12 no.6
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    • pp.648-654
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    • 2018
  • Background/Aims: Dual priming oligonucleotide-based multiplex polymerase chain reaction (DPO-based PCR) can detect the presence of clarithromycin resistance without culture. The aim of this study was to investigate the cost-effectiveness of DPO-based PCR for Helicobacter pylori eradication. Methods: From 2015 to 2016, medical records of patients who received H. pylori eradication therapy were analyzed. Patients were divided into two groups: tailored group patients who were treated based on DPO-based PCR and empirical group patients. Eradication rate and medical cost, including diagnostic tests, eradication regimens, and $^{13}C$-urea breath tests, were compared between the two groups. Cost for one successful eradication was calculated in each group. The expected cost of eradication for empirical treatment was investigated by varying the treatment duration and eradication rate. Results: A total of 527 patients were analyzed (tailored group 208, empirical group 319). The eradication success rate of the first-line therapy was higher in the tailored group compared to that in the empirical group (91.8% vs 72.1%, p<0.01). The total medical cost for each group was $114.8{\pm}14.1U.S.$ dollars (USD) and $85.8{\pm}24.4USD$, respectively (p<0.01). The total medical costs for each ultimately successful eradication in the tailored group and in the empirical group were 120.0 USD and 92.4 USD, respectively. The economic modeling expected cost of a successful eradication after a 7- or 14-day empirical treatment was 93.8 to 111.4 USD and 126.3 to 149.9 USD, respectively. Conclusions: Based on economic modeling, the cost for a successful eradication using DPO-based PCR would be similar or superior to the expected cost of a successful eradication with a 14-day empirical treatment when the first-line eradication rate is ${\leq}80%$.