• Title/Summary/Keyword: dry-out

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THE VERIFICATION OF THE MTT ASSAY ON THE VIABILITY OF PERIODONTAL LIGAMENTAL CELLS IN RAT MOLARS THROUGH THE HISTOLOGIC EXAMINATION (쥐치아 치근면의 치주인대세포의 활성도를 평가하는 방법으로 MTT검색법의 적절성에 대한 조직학적인 검증)

  • Kim, Hyun-Ki;Kim, Eui-Seoung;Choi, In-Bok;Kim, Jin;Lee, Seung-Jong
    • Restorative Dentistry and Endodontics
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    • v.28 no.5
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    • pp.385-391
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    • 2003
  • The purpose of this study is to examine the viability of PDL cells in rat molars by using MTT assay and to verify the MTT assay through the histologic observation. Thirty of Sprague-Dawley white female rats of 4-weeks old with a body weight of about 100 grams were used. Groupings are as follows : Immediate Group : Positive control group(n=10)-after extraction immediately. Dried Group : Negative control group(n=10)-after drying for an hour under warm dry. $ViaSpan^{\circledR}$ Group : 1hour $ViaSpan^{\circledR}$ group(n=10)-after storing in $ViaSpan^{\circledR}{\;}at{\;}4^{\circ}C$ for 1hour. Ten teeth of each group were treated as same as above and replanted to the original socket of experimental animals. After two weeks of replantation. all the experimental animals were sacrificed. And after fixation, extracted maxillary jaw was dimineralized. After it was embedded in paraffin. serial section by $5\mu\textrm{m}$ was carried out and for construction of specimen, hematoxylin-eosin dye was used. The mean MTT measurement of immediate group(positive control) is 2.81 and the mean measurement of dried group(negative control) is 0.98 which is significantt differnt(P<0.05), The mean measurement of $ViaSpan^{\circledR}$ group is 2.65 and there is significant difference between dried group and $ViaSpan^{\circledR}$ group(P<0.05), However, there is no difference between immediate group and $ViaSpan^{\circledR}$ group. The average resorption points of immediate group is 3.03 points. In the dried group, average 6.44 points resorption and 2.68 points showed resorption in the $ViaSpan^{\circledR}$ group. Unlike with MTT assay, there was no significant difference between the immediate group and $ViaSpan^{\circledR}$ group. The usage of MTT assay as a viable cell marker may give us a better indication of the maintenance of periodontal ligament cell vitality.

Anaerobic Acid and Methane Fermentation using Paper Wastes (폐지를 이용한 혐기성 산 발효 및 메탄발효 특성)

  • 조건형;김중곤;김성준;김시욱
    • KSBB Journal
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    • v.18 no.4
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    • pp.318-323
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    • 2003
  • This study was carried out to investigate the possibility of reusing newspaper or paperbox waste by methane fermentation. When 15 g of newspaper and box wastes were digested separately for 24 days by batch fermentation, the amount of total organic acids produced were 2461 and 4978 mg/L, respectively. The tCOD removal rates were found to be 60.9 and 62.4%, respectively. In addition, the removal efficiencies of total solid were 34.8 and 33.4%, and those of volatile solid were 40.0 and 39.2%, respectively. During this period, the amounts of biogas produced were 6.95 and 6.43 L. In a semicontinuous reaction, tCOD removal efficiencies for newspaper and box wastes were 64.7 and 65.0%, respectively, after 14 days of digestion. After 25 days, which were needed to stabilize the methane fermentation, the amounts of biogas produced daily were 0.31 and 0.30 L/g.dry wt, respectively. Methane contents were 57.3 and 56.2%, respectively, and the pHs in the anaerobic acidogenic and methanogenic fermenters were 5.0 and 7.5, respectively.

Weather and Climatic Environment of Seoul Area in South Korea during 1623~1800, Reconstructed from 'The Daily Records of Royal Secretariat of Joseon Dynasty(承政院日記)' (1623~1800년 서울지역의 기상기후 환경 -'승정원일기'를 토대로-)

  • LEE, Joon Ho
    • Journal of the Korean association of regional geographers
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    • v.22 no.4
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    • pp.856-874
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    • 2016
  • This study aims to figure out the weather and climate environment of Seoul area in S. Korea during 1623~1800, which has not been studied so far, by using daily records of weather conditions and meteorological phenomena in the Daily Records of Royal Secretariat of Joseon Dynasty(承政院日記) together with records of abnormal weather conditions and natural disasters in the Annals of the Joseon Dynasty(朝鮮王朝實錄). During 1500~1760 as a period of the Little Ice Age it was generally cold and dry, particularly cool summers of Seoul area. Changes in weather conditions and meteorological phenomena and climate changes appeared prominently at around 1650, 1710, 1770. The annual numbers of rain days and of snow days began to change largely in the 1640s. The rain(and snow) days reduced significantly in the 1710s~1650s, but increased sharply in the 1710s and later. The rain days in summer rapidly increased after the late 1710s, while the snow days greatly reduced after the mid 1770s. The cloudy days around the 1710s greatly reduced in summer, while slightly increased in winter. The hail days increased significantly in the late 1720s and lasted until the 1760s. The fog days began to reduce after 1770 to the fewer days than the climatic normals of 1981~2010. These times are overall consistent with findings of historical climatological cross-checking data and geophysical biological proxy data, accompanied by a trend of relatively enhanced colder and drier of Seoul area.

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A Study on Mineral Contents in Domestic Mozzarella Cheese (Mozzarella Cheese의 무기질 함량에 관한 연구)

  • Cha, Gwang-Jong;Yeon, Je-Han;Yu, Je-Hyeon
    • Journal of Dairy Science and Biotechnology
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    • v.14 no.2
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    • pp.147-155
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    • 1996
  • This experiment was carried out to find general composition and mineral contents such as Ca, P, Mg, Na, K and Fe of domestic commercials Mozzarella cheese. Twenty-five products of 5 company were analyzed. The results obtained were as fellow : The average contents of moisture, fat, fat in dry matter, protein and ash in Mozzarella cheese were 49.01, 22.54, 44.16, 24.36 and 2.55%, respectively and average pH was 5.29. The average contents of Ca, P, Mg, Na, K and Fe were 696.44, 466.31, 22.08, 310.8, 82.84 and 0.36mg, respectively. Among products, product C was highest in levels of Ca, P and Mg, which were 797.4, 536.6 and 25.5mg, respectively, Na was highest for product A which contained 450.8mg. K and Fe were highest for product B which contained 98.9 and 0.51mg, respectively. Generally, the ratio of Ca to P was 1.5 : 1 and within the proper range. It was found that 645g of Mozzarella cheese was able to fully meet the daily recommendation of minerals including Ca, P and Na.

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Studies on the Behavior of Phosphorus under the Conditions of Transplanting and Direct-sowing Culture in Rice Plants (수도(水稻)의 직파(直播) 및 이식재배(移植栽培) 조건하(條件下)의 인산(燐酸) 소장(消長)에 대(對)하여)

  • Ahn, Hak-Soo
    • Applied Biological Chemistry
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    • v.11
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    • pp.185-188
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    • 1969
  • This experiment was carried out to investigate on the growth, yield behavior of phosphorus at different stage of growth under the different cultivating conditions in rice plants. The results obtained were as follows; 1) The phosphorous contents was increased in the direct-sowing plots of the rooting time and the early stage of tillering, while in the middle and the later stage of growth, the transplanting plots was increased. 2) In the moisture contents, the direct-sowing plots was more increased than the transplanting plot in the rooting time and the early stage of tillering, while in the later stage of growth, the transplanting plots was increased. 3) The plant height and the weight of dry matter were also increased in the direct-sowing plots of the early stage of tillering, on the other hand, after that the transplanting plots was increased. 4) In the yield of grain, the transplanting plots was increased about 15% compared with the direct-sowing plots.

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Basic Studies on the Plant Ecosystem for the Environmental Conservation in Masan District (마산지역의 환경보전을 위한 식물생태계의 기초연구)

  • 이경재;이명우
    • Journal of the Korean Institute of Landscape Architecture
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    • v.13 no.1
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    • pp.79-94
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    • 1985
  • This study was carried out to give basic information about the environmental conservation in Masan District. It included the actual vegetation the degree of human disturbance of vegetation, plant biomass and biomass production. The natural vegetation was nonexistent and the major plant communities of the secondary forest was P. thunbergii-Q. acutissima and Quercus forest. P. thunbergii-Q. acutissima community area was 29.2% of Masan District and the secondary forest and the afforestation area was 48% and 13% of Masan District respectively. The ecological diversity of the plant community was high value in Q. acutissima- P. thunbergii, Q.aliena and Carpinus laxiflora-Q.variabilis communicty. As the investigation of the ecological succession in Masan Forest, P. thunbergii community is edaphic climax and mixed forest will be changed gradually dominant species of Quercus species. It was implied that Genus Quercus hadnever beenso easily taken ever by the Genus Pinus which forms the edaphic climax at vast areas of forest land. In the degree of human disturbance of vegetation, the degree 7 (secondary forest 1) was 52.8% and the green spaces in Masan cover 65%, which nongreen spaces 35 %. Total amount of plant biomass produced from Masan District was estimated to be 160, 470.95 tons. Annual Production of dry matter biomass amounts to be 32,940.64 tons. Estimated amount for O$_2$produced annually from the vegetated area in Masan was 34,856 tons.

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Recovery of Tantalum Anode from Waste Tantalum Condenser by Air Classification (공기분급에 의한 폐콘덴서로부터 탄탈륨 회수)

  • Kim Sang-Bae;Cho Sung-Baek;Cho Keon-Joon;Kim Yoon-Jong;Lee Jae-Chun;Kim Won-Baek
    • Resources Recycling
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    • v.12 no.3
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    • pp.3-12
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    • 2003
  • Physical separation containing grinding, sieving, dry magnetic separation and air classification were carried out in order to recover Ta anode from waste Ta condenser. Roll crusher wat used for the liberation of resin and metals in closed circuit system. The liberation between Ta anode and resin was easily achieved, whereat some of metals did not liberated from the Ta anode when the waste condenser was crushed below 8 mesh. When the crushed sample were divided into 8/10 mesh, 10/18 mesh and -18 mesh, metals was mainly remained in 8/10 mesh in contrast to Ta anode was in +18 mesh. It was shown that resin was more easily crushed rather than metals from the result of that resin content was 71.5% in -18 mesh. The liberation efficiency was different with the input size of the crushed sample and average efficiency was 62.3% due to the locked Ta anode particles. The results of air classification test for the crushed samples showed that optimal air flow are 39㎥/h, 32㎥/h, 20㎥/h. respectively. When the sample were separated with optimal condition, 94.45% Ta anode containing 97.47 wt.% Ta anode, 0.93 wt.% resin. 1.61 wt.% metal was recovered with 49.39 wt.% yield.

Comparative Study on the Control and Removal of Formaldehyde for the Urea-formaldehyde Resin Bonded Plywood -Adhesive control- (요소수지접착합판(尿素樹脂接着合板)의 유리(遊離)포름알데히드 방산(放散)제거 및 조절방법에 대한 비교연구(比較硏究))

  • Lee, Hwa-Hyoung
    • Journal of the Korean Wood Science and Technology
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    • v.9 no.3
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    • pp.16-23
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    • 1981
  • This study has been carried out to make a comparative study for the adhesive control methods specifically developed for application to formaldehyde. The method for formaldehyde determination used in this report is the improved chromo tropic acid determination. The results are summarized as follows: 1. The soaking treatment in aqueous solution of urea wok the most scavenging effect on the formaldehyde release from a plywood sample glued with a urea formaldehyde adhesive, and other removal treatment such as resorcinol, albumine-, and hardener-treatment gave significant reduction too. 2. In glue shear strength of dry test, 2% of resorcinol treatment and soaking treatment showed the highest strength and all the other treatment met the standard, but in hot water soaking test, 2% of resorcinol treatment gave the best results, on the other hand, adding the hardener showed the lowest strength and failed in meeting the standard. 3. Air dried moisture content of all treated plywood met the standard which calls for 13% or bellow. 4. In this comparative study, we can make a strong combination each other or go into the details of one treatment for the best result through the more study.

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Effects of Drying Methods on Content of Active Components, Antioxidant Activity, and Color Values of Saururus chinensis Bail (건조방법에 따른 삼백초의 유효성분 함량, 항산화능 및 색도)

  • Kim, Min-Ja;Kim, In-Jae;Nam, Sang-Young;Lee, Cheol-Hee;Yun, Tae;Song, Beom-Heon
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.1
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    • pp.8-13
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    • 2006
  • This study was carried out to investigate drying methods for keeping freshness and active components of aerial part of Saururus chinensis. Chopped samples were prepared using hot air drying, hot air drying + roasting, presteamed + hot air drying, freeze drying, and sun drying. Drying time was shorter $88{\sim}93%$ in hot air drying and freeze drying than in sun drying. Percentage of dry matter was higher in the order of freeze drying > sun drying > hot air drying, and moisture content in the order of sun drying > hot air drying > freeze drying. The contents of total phenolics and quercetin related substances were greater in the order of freeze drying > hot air drying > sun drying, while were decreased due to treatments before or after hot air drying. Electron donating ability (EDA) was highest in freeze drying, and showed a little difference between hot air drying and sun drying, but was increased because of treatments before or after hot air drying. In color values, the a value was lower in the order of freeze drying < hot air drying < sun drying. The contents of active components of plant parts of hot air dried were higher in the order of flower >leaf > chopped sample > stem. EDA showed similar tendency with those. The a value was lower in the order of leaf < chopped sample < flower < stem. As a result, freeze drying of chopped samples or hot air drying of leaf and flower were effective to maintain qualities of aerial part of Saururus chinensis.

Effect of Organic Compost on Growth and Yield in Scutellaria baicalensis (유기질 퇴비 시용이 황금의 생육 및 수량에 미치는 영향)

  • Kim, Myeong-Seok;Choi, Jin-Gyung;Kim, Hee-Kwon;Chung, Byoung-Jun;Bang, Geuk-Pil;Kim, Joung-Keun;Park, Min-Soo;Ahn, Young-Sup;Kim, Young-Guk;Park, Chung-Berm
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.3
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    • pp.168-172
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    • 2010
  • This experiment was carried out to investigate the effect of several organic compost on the growth and root yield of Scutellaria baicalensis G. with organic cultivation from 2008 to 2009. Four organic compost were used ; Rice bran compost (RC : 1.1-3.6-1.4), Wheat bran compost (WC: 1.1-3.4-1.4), Hangawi compost (HC : 3.7-4.0-1.0), Cheonnyeonjigi compost (CC : 12.4-3.4-5.4) and Fermented chicken droppings compost (FC : 2.9-6.1-3.2). Organic matter, available phosphate and $K_2O$ of soil increased in all plots with the addition of organic compost, specifically in HC, CC and FC application. Treatment of HC, CC and FC application increased emergence rate and improved the growth characters of shoot, root parts of plants compared to those of RC application. Subterranean part yields by different organic compost was high 149~156 kg per 10a as by growth increment of dried root yield. length of main root and diameter of main root. respectively, 17.4~18.4 cm, 12.65~12.94 mm from HC, CC and FC application. There were significantly positive correlation between the growth characters of shoot, root parts of plants, dry root yield in Scutellaria baicalensis G. grown under organic compost application.