• Title/Summary/Keyword: drought-tolerant

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Development of Stress-tolerant Crop Plants

  • Park, Hyung-In;Kang, Jung-Youn;Sohn, Hee-Kyung;Kim, Soo-Young
    • Journal of Plant Biotechnology
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    • 제4권2호
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    • pp.53-58
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    • 2002
  • Adverse environmental conditions such as drought, high salt and cold/freezing are major factors that reduces crop productivity worldwide. According to a survey, 50-80% of the maximum potential yield is lost by these "environmental or abiotic stresses", which is approximately ten times higher than the loss by biotic stresses. Thus, improving stress-tolerance of crop plants is an important way to improve agricultural productivity, In order to develop such stress-tolerant crop plants, we set out to identify key stress signaling components that can be used to develop commercially viable crop varieties with enhanced stress tolerance. Our primary focus so far has been on the identification of transcription factors that regulate stress responsive gene expression, especially those involved in ABA-mediated stress response. Be sessile, plants have the unique capability to adapt themselves to the abiotic stresses. This adaptive capability is largely dependent on the plant hormone abscisic acid (ABA), whose level increases under various stress conditions, triggering adaptive response. Central to the response is ABA-regulated gene expression, which ultimately leads to physiological changes at the whole plant level. Thus, once identified, it would be possible to enhance stress tolerance of crop plants by manipulating the expression of the factors that mediate ABA-dependent stress response. Here, we present our work on the isolation and functional characterization of the transcription factors.n factors.

수도 한발저항성 품종 선발에 관한 연구 (Studies on Screening Rice Cultivars for Drought Resistance I. Screening at Seedling Stage)

  • 서학수
    • 한국작물학회지
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    • 제24권3호
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    • pp.7-12
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    • 1979
  • 748개의 벼 품종을 파종하여 토양수분이 포화된 상태로 30일간 재배한 후 40일간 단수하여 한발을 조장한 다음 각 품종들의 한발에 대한 반응을 검정한 결과는 다음과 같다. 1. 한발처리기간 중 초장의 신장정도는 일본품종들이 가장 컸고 IRRI 도입품종, 한국재래품종 및 Indica / Japonica 품종 순이었다. 2. 한발처리후의 위조정도는 IRRI도입 품종들이 가장 경미하였고 일본도입품종, Indica / Japonica 품종 및 한국재래품종 순이었다. 3. 유묘기 내한성품종으로 원길조, 한양도, YR 52-$_{x}\textrm{BF}_7-67, Satominori, Mamoriaka, 적야난11004, 죽 홍난, IR 937-55-3, IR2735-F_3B-6-2, IR 2871-53-2, $_{x}\textrm{BFI76}^9/Dwan, Khoo Dook Mali-4-2-105, Os6, Palawan, IRAT10, TOS 2583 및 H-l05 등이 발달되었다. 4. 한발처리후의 조장, 조장신장량 및 초장신장율과 위조정도와는 높은 부의 상관이 인정되었다. 5. 한발처리후의 지상부 생체중, 건물중 및 수분함량과 위조정보와는 높은 부의 상관이 인정되었고 특히 수분함량과 위조정도와의 상관이 높았다.(r=0.860, n=21). 6. 한발처리후의 도열병발병과 위조정도와는 높은 정의 상관이 인정되었다. 7. 발아속도 및 처리전초장 (30일묘)과 내한성과는 상관이 없었다.

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Physiological responses of selected Philippine upland rice genotypes evaluated using drought and salinity stress

  • Zapico, Florence;Aguilar, Catherine Hazel;Laniton, Lyn Jean;Lincay, Reygiene;Duldoco, Roman Abdul Kadir;Leandres, Jacy Deneb
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.306-306
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    • 2017
  • Screening for drought and salinity tolerance was undertaken for selected Philippine upland rice landraces during germinative and seedling stages to identify varieties which can potentially be grown in marginally dry and saline soils. While increasing PEG and NaCl concentrations caused obvious signs of injury to all rice genotypes, considerable varietal differences were noted in the nature of responses providing evidence that these genotypes possess broad intraspecific genetic variations for drought and salt tolerance. Inconsistent responses of these varieties during both growth stages highlight complexities involved in stress responses and underscore the futility of utilizing a single stage in the rice plant's life cycle for physiological screening. Notwithstanding these perplexing responses, G_Katiil and Ml-Pilit Tapul were observed to thrive relatively well despite increased salt and drought stress during early growth stages and may therefore possess genes needed in crop improvement efforts for drought and salinity tolerance. While these results do not reflect the entire spectrum of adaptive expression to drought and salinity stress during the life cycle of the upland rice plant, they nonetheless provide an easy, reliable and reproducible method for preliminary identification of drought and salt tolerant rice varieties.

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Identification of Candidate Transcripts Related to Drought Stress using Secondary Traits and qRT-PCR in Tropical Maize (Zea mays L.)

  • Kim, Hyo Chul;Song, Kitae;Moon, Jun-Cheol;Kim, Jae Yoon;Kim, Kyung-Hee;Lee, Byung-Moo
    • 한국작물학회지
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    • 제64권4호
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    • pp.432-440
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    • 2019
  • Global climate change exerts adverse effects on maize production. Among abiotic stresses, drought stress during the tasseling stage (VT) can increase anthesis-silking intervals (ASI) and decrease yield. We performed an evaluation of ASI and yield using a drought-sensitive line (Ki3) and a drought-tolerant line (Ki11) to analyze the correlation with ASI and yield. Moreover, the de novo data of Ki11 were analyzed to find putative novel transcripts related todrought stress in tropical maize. A total of 182 transcripts, with a log2 ratio >1.5, were found by comparing drought conditions to a control. The top 40 transcripts of high expression levels in the de novo analysis were selected and analyzed with PCR. Of the 40 transcripts, six novel transcripts were detected by quantitative real-time PCR (qRT-PCR) using seedling and VT stage samples. Five transcripts (transcripts_1, 12, 34, 35, and 40) were up-regulated in the Ki11 shoot at seedling stage, and transcripts_1, 12, and 40 were up-regulated at the re-watering stage after 12 h of drought stress. The transcripts_32 and 34 were up-regulated at the VT stage. Hence, transcript_34 possibly plays a significant role in drought tolerance during the seedling and VT stages. The transcript_32 was identified as chloramphenicol acetyltransferase (CAT) by Pfam domain analysis. The function of the other transcripts remained unknown. Further characterization of these novel transcripts in genetic regulation will be of great value for the improvement of maize production.

Effects of Water Deficit on Biomass Accumulation and Water Use Efficiency in Soybean during Vegetative Growth Period

  • Kim, Wook-Han;Hong, Byung-Hee;Larry C. Purcell
    • 한국작물학회지
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    • 제45권1호
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    • pp.6-13
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    • 2000
  • Water deficit is the primary constraint of soybean [Glycine max (L.) Merr.] yield, and a physiological understanding of processes affected by water deficit is a key step in identifying and improving drought tolerance in soybean. The objectives of this research were to evaluate biomass and nitrogen accumulation patterns and water use efficiency (WUE) as possible mechanisms associated with the drought tolerance of Jackson. Biomass accumulation of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. For water-deficit treatment, total biomass accumulation was negligible for PI416937, but biomass accumulation continued at approximately 64 % of the well-watered treatment of Jackson. Transpirational losses for Jackson and PI416937 were approximately the same for the water-deficit treatment, indicating that Jackson had superior WUE. Isotopic discrimination of $^{13}$ C relative to $^{12}$ C also indicated that Jackson had higher WUE. Results indicated that increased WUE for Jackson under water deficit showed it was tolerant to drought rather than had an avoidance mechanism.

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내건성 식물생장 촉진 균주인 Glutamicibacter halophytocola DR408의 유전체 분석 (Complete genome sequence of drought tolerant plant growth-promoting rhizobacterium Glutamicibacter halophytocola DR408)

  • 수스미타 다스 니슈;현혜림;이태권
    • 미생물학회지
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    • 제55권3호
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    • pp.300-302
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    • 2019
  • 제천에서의 콩 근권 시료로부터 분리한 Glutamicibacter halophytocola DR408 균주는 내건성 식물생장촉진력을 보이고 있다. 본 연구에서 DR408 균주의 완전한 유전체 서열을 해독한 결과, 유전체의 크기는 3.77 Mbp였으며, G + C 함량이 60.2%였다. 또한 총 3,352개의 유전자 서열과 65개의 tRNA, 19개의 rRNA, 3개의 ncRNA가 존재하였다. 유전체 분석을 통해 식물의 내건성을 향상시킬 수 있는 삼투질 합성과 식물생장촉진 효소를 코딩하는 유전자를 다수 포함하는 것을 확인하였다.

Long-term drought modifies carbon allocation and abscisic acid levels in five forest tree species

  • Umashankar Chandrasekaran;Kunhyo Kim;Siyeon Byeon;Woojin Huh;Ah Reum Han;Young-Sang Lee;Hyun Seok Kim
    • Journal of Ecology and Environment
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    • 제47권4호
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    • pp.241-249
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    • 2023
  • Background: This study analyzed the drought responses of five forest tree species grown in Korean peninsula, Korean fir Abies koreana (Ak), eastern white pine Pinus strobus (Ps), keyaki Zelkova serrata (Zs), tulip tree Liriodendron tulipifera (Lt), and Japanese elm Ulmus japonica (Uj). Physiological (chlorophyll, root collar diameter [RCD]) and biochemical responses (non-structural carbohydrates, proline, lipid peroxidase and abscisic acid [ABA]) of the plants grown under mild (MD) and severe drought (SD) were compared. Results: In this study, three soil moisture regimes: control (100% precipitation), MD (60% reduction in precipitation) and SD (20% reduction in precipitation) were applied. Soil moisture content showed high water content in control site compared to MD and SD. A decline in RCD was found for Korean fir, keyaki, and tulip plants, with eastern white pine and Japanese elm showing no significant decline to the prolonged drought exposure (both MD and SD). Total chlorophyll showed a significant decline in Korean fir and tulip, with the sugar levels indicating a significant increase in Korean fir and keyaki species under SD compared to control plants. Non-significant decline in sugar level was noted for eastern white pine and Japanese elm. High accumulation of ABA, malondealdehyde and proline was noted in Korean fir, tulip, and keyaki under SD compared to control. Signs of tree mortality was only observed in Korean fir under MD (38%) and SD (43%). Conclusions: The observed findings indicate the drought responses of five tree species. The majority of the morpho-physiological (especially mortality) and biochemical variables assessed in our study indicate superior long-term drought resistance of Ps and Uj compared to the highly sensitive Ak, and moderately sensitive Lt and Zs. The results provided will help species selection for afforestation programs and establishment of sustainable forests, especially of drought-tolerant species, under increased frequency and intensity of spring and summer droughts.

Variation in root system developmental responses of irrigated and rainfed philippine rice varieties to water stressed environments

  • Cabral, Maria Corazon J.;Niones, Jonathan M.;Suralta, Roel R.;Yamauchi, Akira
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.230-230
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    • 2017
  • About 200 rice varieties for irrigated and rainfed lowland ecosystems were released in the Philippines, which were bred for improving yield under favorable conditions. Root plasticity plays key roles in maintaining crop productivity under abiotic stressed conditions. We hypothesized that some of these varieties possess root plasticity traits in response to water stressed conditions. This study aimed to evaluate the root system development and dry matter production of 14 randomly selected rice varieties (6 irrigated lowland and 9 rainfed varieties) under progressive drought (PDR) and soil moisture fluctuations (SMF) stress conditions. Two experiments were done in rootbox and line source sprinkler systems (LSS). Each of the varieties was subjected to well-watered (WW), PDR and SMF conditions during vegetative stage in rootbox system while the same genotypes were subjected to different intensities of drought stress under LSS. Under rootbox system, PDR and SMF significantly reduced shoot dry matter production in all varieties relative to their WW controls. Among varieties, NSIC Rc238 (irrigated lowland) showed the least reduction in shoot dry weight (SDW) in both PDR (by 11.8%) and SMF (by 26.9%) conditions. Less reductions in SDW of NSICRc238 were partially attributed to the promotion of L-type lateral roots, thus increasing total lateral root length by 24.2% and 30.7% under PDR and SMF, respectively. In LSS, SDW of NSIC Rc238 under mild drought stress (16-21% soil moisture content (SMC) had 31.8% reduction relative to its WW control (${\geq}22%SMC$) and had lower sensitivity drought index. Compared with the IR64 susceptible check and NSIC Rc9 tolerant check, NSIC Rc238 had higher SDW by 90.8% and 38.6%, respectively. Furthermore, no rainfed lowland varieties included in the experiment performed well under different water stress treatments. The results implied that some other irrigated lowland rice varieties may also possess drought dehydration avoidance root plasticity traits under water-stressed growing environments.

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