• 제목/요약/키워드: drop-culture

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In Vitro Development of Porcine Parthenogenetic Embryos under the Oil-free Culture System

  • Park, Sang-Kyu;Choi, Young-Ju;Roh, Sang-Ho
    • Journal of Embryo Transfer
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    • v.25 no.4
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    • pp.259-262
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    • 2010
  • Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

Effect of Heat Shock on Culture Method and Essential Amino Acid Free Medium of IVM-Derived Bovine Embryo (체외성숙된 소 배에서 배양방법과 필수 아미노산 무첨가 배지에서의 온도충격의 영향)

  • 김지철;김재영;주재홍;윤산헌;이상민;이상진;김재명;송해범;박흠대
    • Journal of Embryo Transfer
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    • v.16 no.1
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    • pp.41-46
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    • 2001
  • This study was carried out to evaluate the effect of culture methods on development of embryos with each developmental stage after heat shock in bovine oocytes. The results obtained were as fellows. 1. The culture method after heat shock on development of embryos was better drop-culture than co-culture. 2. The medium without amino acids were not effect of heat sock on development of embryos but it was in need of amino acid during formation of blastocyst.

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Effects of biological Factors on In Vitro Production of Hanwoo Embryos (한우 난포란 유래 배반포의 체외생산을 위한 생물학적 요인들의 영향)

  • 박흠대;김재영;주재홍;공건오;윤산현;공일근;이상민;이상진;송해범
    • Journal of Embryo Transfer
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    • v.15 no.2
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    • pp.129-136
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    • 2000
  • This study was carried out to investigate the effect of biological factors on the in vitro production(IVP) of bovine oocytes for development of simple culture methods and medium. Oocytes from the slaughterhouse ovaries were matured and fertilized using general protocol and this study was examined if there were necessary to co-culture, media change, media type and embryo density. This results were as follows: 1. The development rate according to co-culture with cumulus cells and non co-culture as drop culture was not significantly different in cleavage (68.9 vs 71.7%), 8-cell stage (41.2 vs 44.1%) and blastocyst stage (12.2 vs 13.8%), respectively (p<0.05) 2. The blastocyst development rates in YS and CRIaa were higher than that in TCM199 (12.4, 10.4$ vs 3.7%), but the cleavage (69.0, 77.8 and 61.0%) and 8-cell stage (31.7, 37.0 and 35.7%) development accoring to YS, TCM199 and CRIaa ws not significantly different, respectively (p<0.05). 3. There was no significantly different in cleavage (62.6, 59.5 and 61.2%), 8-cell(34.7, 37.9 and 34.0%) and blastocyst (9.5, 11.6 and 12.8%) development among medium change time as control, Group I and Group II, respectively (p<0.05). 4. Blastocyst formation of 8-cell stage according to embryo density was not significantly different in 1, 10 and 25 embryos (27.3, 22.5 and 34.0%), respectively (p<0.05). These results indicated that simple culture system could reduce bovine IVP embryos as drop culture as non co-culture system, high density embryo (25 embryos/50 $\mu$1 drop). YS defined medium and no medium change for whole culture period, although other biological factors need to examine in order to produce efficient IVP bovine embryos.

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Characteristics of Adult Women's Body Somatotype according to Drop Values (성인 여성의 드롭 치수에 따른 체형 특성 연구)

  • Yoon, Ji-Won;Suh, Mi-A
    • The Research Journal of the Costume Culture
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    • v.20 no.4
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    • pp.529-534
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    • 2012
  • An analysis of adult women's somatotypes classified by drop values can provide important preliminary data in determining standard clothing sizes for mass production of ready-made clothes. This is because the values are based on measurements of girth, which is essential information in the size-setting process. Adult women's body types are classified according to drop values in a standard clothing size system adopted by Korea and several other countries. This study aims to identify somatotype characteristics of adult women aged 20 to 69 by using five types of drop values: bust-waist, hip-waist, hip-bust, abdomen-waist, and hip-abdomen. Statistical cluster analysis of collected data revealed four somatotypes among adult women. Type R, with a developed lower body, accounted for 36.6%, and its bodyline was found to be closest to the average adult woman. Type X, constituting 25.4%, had the curviest contours of all and relatively large hip measurements, with the thinnest figure. Flat abdomen and hips characterized type Y, which accounted for 18.9%. Lastly, 19.0% fell into type H, the most obese figure, in which abdomen girth exceeded hip measurements. Since type H has excess fat deposits in the abdomen, not only hip measurements but also abdomen girth should be considered when designing top longer than torso-length and bottoms.

Optimization of Embryo Density and the Volume of Culture Medium for an Improvement of Mouse Parthenogenetic Embryo Development

  • Roh Sangho;Choi Young-Joo;Min Byung-Moo
    • Reproductive and Developmental Biology
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    • v.29 no.3
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    • pp.145-147
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    • 2005
  • Autocrine or paracrine mediators released by the early embryo are implicated in the support of embryonic development. Their mechanisms and optimal embryo density in the medium, however, are uncertain. This study was conducted to establish the optimal embryo density and culture medium volume in mouse parthenogenetic embryo culture. In experiment 1, culture of parthenogenetirally activated oocytes at a concentration of $2{\~}4$ embryos/${\mu}L$ significantly improved development to the blastoryst stage ($72{\%}{\leq}$) compared with culture at the lower ($0.2{\~}1$e mbryos/${\mu}L,\;0\~37.5\%$) and the higher ($5{\~}6$ embryos/${\mu}L,\;30\~53\%$) concentration for 120 h when the oocytes were cultured in a 5 ${\mu}L$ drop under mineral oil In experiment 2, the embryos cultured at a concentration of $2{\~}4$ embryos/${\mu}L$ in a 10 ${\mu}L$ drop ($81.1{\%}$) showed significantly higher blastocyst rates than those in a 5 ${\mu}L$ drop ($68.5{\%}$). This study optimizes in vitro culture condition by modifying embryo density and the volume of culture medium It may give appropriate level of autocrine and/or paracrine factors to enhance viability and subsequent normal development of mouse parthenogenetic embryos in vitro.

Garment Sizes Analysis on Target Ages and Body Shapes (신체 특성에 따른 타겟 연령별 치수 호칭 설정 - KS 규격 및 신체 치수 분석을 바탕으로 -)

  • Kang, Yeo-Sun
    • The Research Journal of the Costume Culture
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    • v.15 no.1 s.66
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    • pp.137-151
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    • 2007
  • This study was performed to narrow down the Sizing System for Female Adult's garments(KS K 0051) following age target and body shapes. The study was based on the sizing system and the anthropometric measurements, called Size-Korea, which were surveyed in the years 2003 and 2004. At first, the subjects of the survey were classified into 4 age groups, $18{\sim}24,\;25{\sim}34,\;35{\sim}49\;and\;50{\sim}59$, based on the most significant differences of body sizes. Secondly, the ranges of four key dimensions - height, bust, waist and hip-of normal sizes were identified through analyzing Rohrer index and percentile. The selected sizes within the ranges were grouped by the drop which was the subtraction of bust girth from hip girth. In conclusion, there was the most frequent drop for each age group; 9cm drop for the $18{\sim}24$ years old group, the 6cm drop for the $25{\sim}34$ years old and $35{\sim}49$ years old and 3cm drop for $50{\sim}59$ years old. The upper body garment sizes of the same drop and target ages could be merged to 10 to 12 sizes, which were remarkably smaller than the Female Adult garment sizing system, and further to 2-5 sizes, considering the percentile importance. However, $50{\sim}59$ years old group needed to be analyzed in different ways, because the number of subjects of normal size in the age decreased rapidly, that the normal sizes for them were very limited. In addition, the reference body sizes of each garment size were apparently different when they were compared to each age groups. These basic data for garment size specification could be usefully adapted for the apparel companies' own sizing system according to their age target and body shapes, to achieve efficient production and better fit.

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Characteristics of Somatotype Classified by the Drop Value of Middle-Aged Women (중년 여성의 드롭치에 따른 체형 특성 연구)

  • Yoon, Ji-Won;Suh, Mi-A
    • The Research Journal of the Costume Culture
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    • v.17 no.6
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    • pp.939-946
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    • 2009
  • This study is to classify the shape of the body by means of the drop value of the middle-age women. Drop value is based on the circumstances of the body deciding the dimentions of the clothes. Therefore, the study based on the drop value seems to decided the measurement of the clothes in making mass production of ready made clothes. In order to put measure items on this study of the value on 'The fifth survey of body measure of Korean', select 785 people in middle-aged women aged from 40 to 59. And considering the characteristic of upper-drop and lower-drop, and swelling belly by accumulated fat, selected five items(Bust Circumstance - Waist Circumstance, Hip Circumstance - Waist Circumstance, Hip Circumstance - Bust Circumstance, Waist Circumstance(Omphalion) - Waist Circumstance, Hip Circumstance - Waist Circumstance(Omphalion)). The result of the study is summarized as follows. Type 1, M type of the body showed the distribution of 32.5% and belonged to low body development style. The line of human body is the nearest of the four bodies in middle-age woman out of whole shapes of 4 types and the line of human body is close to middle-age woman. Type 2, X type of the body showed the distribution of 40.8% and the line of human body remarkably showed. Type 3, H type of the body showed the distribution of 12.4% and the line of human body appears least. This group was larger than any other group in having more fat in the belly above the circumstance of the hip than other groups. Type 4, Y type of the body showed the distribution of 14.4%. This type was upper half of body development style, waist was slender, and there was seldom the bend around the circumstance of belly and hip.

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Men's and women's body types in the global garment sizing systems

  • Chun, Jongsuk
    • The Research Journal of the Costume Culture
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    • v.20 no.6
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    • pp.923-936
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    • 2012
  • Apparel companies define their target customers to integrate consumers' needs into their product development processes. The sizing standards play a significant role in ready-to-wear garment business. Consumers' body build and sizes are different according to gender, age, and body type. The consumers' morphological feature of the one geographical area has changed with immigration, aging, and lifestyle change. In this study the way of defining body types in the standard garment sizing systems published in USA., UK, Germany, Japan, and Korea were compared. The results of this study show that most of the systems classified the body types by the index value. The chest-waist drop value was used for men's body type classification. Women's body types were defined by hip proportion. The hip-bust drop value was used for it. German and European garment sizing systems provide a wide range of men's body types. US men's garment sizes are developed for very conservative body type. US women's garment sizing system has had clearly defined women's body types. The Misses body types projected in the US garment sizing system had changed as women's waist girth got bigger compared to the past. In 2011 the US Misses sizes were divided into Curvy Misses size and Straight Misses size by the hip-waist drop value. The Curvy Misses sizes have smaller waist girth and larger hip girth than the Straight Misses sizes.

Analysis of Differences in Muscle Activity according to Badminton Stroke Movements (배드민턴 스트로크 동작에 따른 근활성도 차이 분석)

  • Kim Hwi-Tae;Kim Ki-Hong;Jeong Huan-Jong;Kim Byung-Kwan
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.2
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    • pp.519-524
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    • 2023
  • The purpose of this study is to construct basic data for efficient technical training by investigating the difference in muscle activity during badminton technical movements involving jump motions similar to game situations. Seven male badminton players were randomly assigned to perform smash, drop, and clear techniques, and electromyograms were measured during the implementation of three technical movements. Measured EMG was calculated by RMS and one-way ANOVA was performed. The muscle activity of the smashing motion did not show any significant difference according to the site. In drop motion, activity of PM in the upper extremity muscles was lower than that of BC and ECR, and FCR activity was lower than that of EC. The activity of ECR was higher than that of PM and FCR. The activity of ES in trunk muscles was lower than that of RF and GM. RF activity of lower extremity muscles was higher than that of ES and BF. In clear motion, the activity of TC in upper extremity muscle was higher than FCR. The activity of ES in trunk muscles was lower than that of BF. RF activity of lower extremity muscles was higher than that of BF, and BF activity was lower than that of RF and GM. The activity of GM was higher than that of BF. As for muscle activity according to badminton skills, smash and drop motions were higher than clear motions in FCR, and clear motions were higher than smash and drop motions in RA. In conclusion, it is considered that muscle activity during the badminton game is different according to the characteristics of each skill, and FCR can affect the smash and drop, and RA can affect the clear motion.