• Earthquakes and Structures
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• v.12 no.1
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• pp.1-12
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• 2017

Historical earthquakes have shown that successive seismic events may occur in regions of high seismicity. Such a sequence of earthquakes has the potential to increase the damage level of the structures, since any rehabilitation between the successive ground motions is practically impossible due to lack of time. Few studies about this issue can be found in literature, most of which focused their attention on the seismic response of SDOF systems or planar frame structures. The aim of the present study is to examine the impact of seismic sequences on the damage level of 3D multistorey R/C buildings with various structural systems. For the purposes of the above investigation a comprehensive assessment is conducted using three double-symmetric and three asymmetric in plan medium-rise R/C buildings, which are designed on the basis of the current seismic codes. The buildings are analyzed by nonlinear time response analysis using 80 bidirectional seismic sequences. In order to account for the variable orientation of the seismic motion, the two horizontal accelerograms of each earthquake record are applied along horizontal orthogonal axes forming 12 different angles with the structural axes. The assessment of the results revealed that successive ground motions can lead to significant increase of the structural damage compared to the damage caused by the corresponding single seismic events. Furthermore, the incident angle can radically alter the successive earthquake phenomenon depending on the special characteristics of the structure, the number of the sequential earthquakes, as well as the distance of the record from the fault.

• Microbiology and Biotechnology Letters
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• v.46 no.2
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• pp.145-153
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• 2018

Among the carotenoid biosynthesis genes, crtI gene encodes the phytoene desaturase (CrtI) enzyme, and phytoene desaturase convert phytoene to lycopene. Phytoene desaturase is involved in the dehydrogenation reaction, in which four single bonds in the phytoene are introduced into a double bond, eliminating eight hydrogen atoms in the process. Phytoene desaturase is one of the key regulating enzyme in carotenoid biosynthetic pathway of various carotenoid biosynthetic organisms. The crtI gene in genomic DNA of Paracoccus haeundaensis was amplified and cloned into a T-vector to analyze the nucleotide sequence. As a result, the crtI gene coding for phytoene desaturase from P. haeundaensis consists of 1,503 base pairs encoding 501 amino acids residues. An expression plasmid containing the crtI gene was constructed, and Escherichia coli cells containing this plasmid produced the recombinant protein of approximately 55 kDa, equivalent to the molecular weight of phytoene desaturase. The expressed protein in cell lysate showed enzymatic activity similar to phytoene desaturase. Phytoene and lycopene were analyzed by HPLC and measured at wavelength of 280 nm and 470 nm, respectively. The $K_m$ values for phytoene and NADPH were $11.1{\mu}M$ and $129.3{\mu}M$, respectively.

• Journal of KIISE:Information Networking
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• v.34 no.3
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• pp.156-167
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• 2007

In this paper, we develop the model based prediction algorithm for Variable-Bit-Rate(VBR) video traffic with regular Group of Picture(GOP) pattern. We use multiplicative ARIMA process called GOP ARIMA (ARIMA for Group Of Pictures) as a base stochastic model. Kalman Filter based prediction algorithm consists of two process: GOP ARIMA modeling and prediction. In performance study, we produce three video traces (news, drama, sports) and we compare the accuracy of three different prediction schemes: Kalman Filter based prediction, linear prediction, and double exponential smoothing. The proposed prediction algorithm yields superior prediction accuracy than the other two. We also show that confidence interval analysis can effectively detect scene changes of the sample video sequence. The Kalman filter based prediction algorithm proposed in this work makes significant contributions to various aspects of network traffic engineering and resource allocation.

• The Korean Journal of Mycology
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• v.26 no.2 s.85
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• pp.256-264
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• 1998

The 18S rDNA sequences of Tricholoma matsutake (TM=T. caligatum var. nauseoum) collected in Korea were analyzed for the ectomycorrhizal fungi in the roots of Pinus densiflora. The 514 base pairs of rDNA region were synthesized by UF-5 and UR-6 primers, and double checked in the base pair. The sequence of four strains synthesized were all identical in this work, but different from those done by the previous workers. The basidiocarps collected in this work. were identified to T. matstake after searching the 18s rDNA by the BLAST in NCBI. Only several base pairs of 18S rDNA analyzed from other related basidiocarps were different from our analyses of 18S rDNA. The dendrogram were made based on the sequences of the 514 bp 18S rDNA by CLUSTAL-X alignment program. The groupings of the species at the level of genus in the dendrogram were well constructed.

• Composites Research
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• v.26 no.5
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• pp.279-288
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• 2013

In this paper, the delamination and deformed FSS caused by residual stresses in the Loop type FSS embedded composites and the transmission characteristic changes due to deformation of FSS by residual stresses were studied. FSS may have different electromagnetic characteristics depending on the type of element, design variables, and array. Therefore, design variables of square loop FSS embedded composites structures were determined to obtain the transmission characteristic for X-band (8~12 GHz). Then the design variables of other types of loops (triangular loop and circular loop) were determined based on the dimensions of square loop. Thereafter, the residual stresses and transmission characteristics of FSS embedded composite structures with various single and double loop FSS's, and stacking sequence of composite laminates were compared.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.39 no.2
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• pp.69-80
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• 2002

In this paper, signed-rank based nonparametric detectors are used for direct sequence code division multiple access pseudo-noise code acquisition systems in frequency-selective Rician fading channels. We first derive the locally optimum rank detector, and then propose the locally suboptimum rank (LSR) and k-th order modified signed-rank (MSRk) detectors using approximate score functions. We compare the serial and hybrid parallel double-dwell schemes using the LSR and MSRk detectors with those using the conventional squared-sum (SS) using the cell averaging constant false alarm rate processor and modified sign detectors. From the simulation results, it is shown that the LSR and MSRk detectors perform better than the SS detector using the cell averaging constant false alarm rate processor.

• Journal of fish pathology
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• v.5 no.2
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• pp.143-152
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• 1992

Metallothionein is an essential and common protein to regulate the intracellular concentration of heavy metals, which exist in most organisms from bacteria to vertebrates. Although the detailed function of metallothianein has not been fully identified until yet, it may be involoved in the cellular protection against the heavy metal toxicity and in the global regulation of several other genes and the expression of metalloproteins. We have cloned the full cDNA clone of metallothionein gene in Channel Catfish by Reverse Transcriptase-Polymerase Chain Reaction(RT-PCR) starting from poly(A)-containing mRNAs. All PCR fragments have been subcloned into EcoRV site of pBluescript SK+ and dT-tailed at Smal site of pUC19, then PCR products are recovered by the double digestion of recombinant plasmids wiht EcoRI and HindIII, which are adjacent to EcoRV site in multicloning sites or by rapid PCR screening. The nucleotide sequence analysis of pMT150(one of the PCR clones) showed high homology with several other piscine metallothionein cDNA genes.

• Fisheries and Aquatic Sciences
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• v.18 no.1
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• pp.99-107
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• 2015

A natural hybrid of a probable intergeneric mating between the striped shiner Pungtungia herzi and the stone morocco Pseudorasbora parva (Cypriniformes: Cyprinidae) was captured in the Geumho River, a tributary of the Nakdong River basin in Korea. Morphological characters and DNA sequences were analyzed to verify its hybrid state and identify the parentage of its parent species. The hybrid exhibited a phenotypic intermediacy between the two parent species in the number of vertebrae and the mouth shape. Out of 1,488 base pair (bp) positions of the nuclear recombination activating gene 1 gene (rag1), which has a biparental mode of inheritance, 41-bp substitutions were detected between the two parent species, whereas an electropherogram of the hybrid displayed polymorphic double peaks at all of the base positions, along with one additional one, strongly indicating its hybrid state. Meanwhile, sequence comparison of the mitochondrial cytochrome b gene (mt-cyb) (1,140 bp), which has a maternal mode of inheritance, showed only 5-22-bp differences (97.6-99.5% identities) between the hybrid and Pu. herzi, but as many as 158-168-bp differences (85.2-86.1% identities) between the hybrid and Ps. parva, clearly indicating Pu. herzi as the maternal species. Thus, combined analyses of independent data sets (i.e., morphology and nuclear and mitochondrial DNA sequences) offered convincing evidence for the hybrid state of a naturally occurring hybrid resulting from intergeneric mating between a female Pu. herzi and a male Ps. parva.

• Applied Chemistry for Engineering
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• v.5 no.3
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• pp.509-516
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• 1994

Liquid phase hydrogenation come into use for the removal process of unsaturated hydrocarbon such as croton aldehyde. The croton aldehyde is generated in a very small amount as by-product in the ethanol production, and it is converted into n-butanol through hydrogenation. Liquid phase hydrogenation is low energy consumption process as compared with gas phase hydrogenation. The nickel catalyst is selected with respect to the economic aspect such as durability and cost. The analysis of the conversion were performed by method of the PMT(permangante time) test. The PMT was sharply decreased as the initial concentrations of croton aldehyde in the ethanol solution were increased. The hydrogenation of croton aldehyde to n-butanol was carried out in sequence after the saturation of the carbon-carbon double bond. The formation of both butyraldehyde and n-butanol followed zero order kinetics. Within expermental conditions the PMT gets longer as reaction temperature goes higer and as LHSV becomes slower, while the reaction pressure has almost no relation with PMT.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.3
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• pp.324-329
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• 2014

To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to create targeted mutations and enriched using a reporter plasmid system. The reporter expresses RFP and eGFP simultaneously when ZFN-mediated site-specific mutations occur. Thus, double positive cells ($RFP^+/eGFP^+$) were selected and used for somatic cell nuclear transfer. Two types of reporter based enrichment systems were used in this study; the cloned embryos derived from cells enriched using a magnetic sorting-based system showed better developmental competence than did those derived from cells enriched by flow cytometry. Mutated sequences, such as insertions, deletions, or substitutions, together with the wild-type sequence, were found in the cloned porcine blastocysts. Therefore, genetic mutations can be achieved in cloned porcine embryos reconstructed with ZFN-treated cells that were enriched by a reporter-based system.