• Journal of the Korean Society of Tobacco Science
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• v.19 no.1
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• pp.5-10
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• 1997

Three strains of W isolated from tobacco, tomato and Pepper plants in Korea were characterized based on biological response, serological relationship, and peptide mapping of the capsid Proteins. The strains designated as TMV-common, TMV-Pepper, and TMV-tomato could be distinguishable by different visual symptoms on 3 varieties of tobacco, one variety of tomato and Pepper for each among 27 plant specieces. Serological relationships were examined by agar gel double diffusion test. Only traceable or weak reaction was observed in the incompatible antigen-antibody combinations. The Pepper strain, however, showed trace in reaction with other two antisera. Peptide maps of the capsid proteins digested by V8 protease or by trypsin were also distinguishable, suggesting differences in composition and/or sequence of the amino acids among the strains.

• Journal of the Korea Society for Simulation
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• v.14 no.2
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• pp.25-34
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• 2005

Determination of component mounting sequence on printed circuit board assembly process is a typical NP-hard problem. It is a kind of traveling salesman problems, but it has one more hard to meet constraint of matching component type per mounting position as well as searching the shortest path. An efficient method is developed by separation of crowed area and allowing up to two slots per component type. A simulation model is constructed using Visual C++ for evaluation of the suggested heuristic.

• Animal cells and systems
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• v.6 no.4
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• pp.311-315
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• 2002

The RAD4 gene is essential for nucleotide excision repair in Saccharomyces cerevisiae. It has been known that the deduced amino acid sequence of Rad4 protein contains three DNA-dependent ATPase/helicase motifs. To determine the biochemical activities and functional role of RAD4 the Rad4 protein was expressed and purified. Immunoblot analysis showed a specific band of 21 kDa, which was well-matched with the size of open reading frame of the RAD4 gene. The purified Rad4 protein had no detectable helicase activity. However, the protein could interact with double stranded oligonucleotides, as judged by mobility shift assay. This result suggests that the Rad4 protein is a DNA binding protein.

• Journal of Korean Institute of Industrial Engineers
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• v.24 no.4
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• pp.551-558
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• 1998

In this paper, a job shop scheduling system is developed which can cope with the changes of shop floor status with flexibility. This system suggests near optimal sequence of operations by using Genetic Algorithm which considers alternative process plans. The Genetic Algorithm proposed in this paper has some characteristics. The mutation rate is differentiated in order to enhance the chance to escape a local optimum and to assure the global optimum. And it employs the double gene structure to easily make the modeling of the shop floor. Finally, the quality of its solution and the computational time are examined in comparison with the method of a Simulated Annealing.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.53 no.5
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• pp.286-292
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• 2004

In this study, an integrated microelectrode array was fabricated on glass slide using microfabrication technology. Probe DNAs consisting of mercaptohexyl moiety at their 5-end were spotted on the gold electrode using micropipette or DNA arrayer utilizing the affinity between gold and sulfur. Cyclic voltammetry in 5mM ferricyanide/ferrocyanide solution at 100 ㎷/s confirmed the immobilization of probe DNA on the gold electrodes. When several DNAs were detected electrochemically, there was a difference between target DNA and control DNA in the anodic peak current values. It was derived from specific binding of Hoechst 33258 to the double stranded DNA due to hybridization of target DNA. It suggested that this DNA chip could recognize the sequence specific genes. It suggested that multichannel electrochemical DNA microarray is useful to develop a portable device for clinical gene diagnostic System.

• Proceedings of the KIEE Conference
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• 2006.07c
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• pp.1324-1326
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• 2006

In this study, an integrated microelectrode array was fabricated on glass slide using microfabrication technology. Probe DNAs consisting of mercaptohexyl moiety at their 5-end were spotted on the gold electrode using micropipette or DNA arrayer utilizing the affinity between gold and sulfur. Cyclic voltammetry in 5mM ferricyanide/ferrocyanide solution at 100 mV/s confirmed the immobilization of probe DNA on the gold electrodes. When several DNAs were detected electrochemically, there was a difference between target DNA and control DNA in the anodic peak current values. It was derived from specific binding of Hoechst 33258 to the double stranded DNA due to hybridization of target DNA. It suggested that this DNA chip could recognize the sequence specific genes. It suggested that multichannel electrochemical DNA microarray is useful to develop a portable device for clinical gene diagnostic system.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 1998.05a
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• pp.276-281
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• 1998

본 논문에서는 온라인 전자현금 시스템을 설계하고 개발한다 개발된 전자현금 시스템은 공개키 알고리즘, 맹목 서명, tut-and-choose등과 같은 다양한 암호화 기법들이 사용되어 안전성을 높였으며 특히, 전자현금의 이중 사용(double spending)의 효과적인 방지를 위해 2중 은행 구조(2-level bank structure)를 취함으로써 통신망을 오가는 트래픽의 양을 많이 줄이고자 하였다. 이 구조의 가장 큰 특징을 각 은행마다 그 은행에서 발행하는 전자현금을 구분하기 위해서 전자 현금의 정보에 들어 있는 일련번호(sequence number)의 값을 은행별로 범주화한다는 점과, 이를 지원하기 위해서 각 은행마다 Cash Clearing Agent라는 모듈을 가진다는 점이다.

• Proceedings of the KIPE Conference
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• 2015.07a
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• pp.291-292
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• 2015

본 논문은 계통과 연계된 3상 전압원 인버터를 기반으로 한 BESS의 능동 단독 운전 검출 방법을 제안한다. 계통 전압의 불평형에서도 안정적으로 위상을 추종할 수 있는 DDSRF_PLL(Decoupled Double Synchronous Reference Frame_PLL)방식을 적용 하였으며, 검출된 위상각 정보를 통해 정상분 전류 제어기와 역상분 전류 제어기를 독립적으로 제어할 수 있게 된다. 이를 위해 IEEE 1547과 UL1741에서 제시하는 단독 운전 기준 시험 회로를 구성하여 PSCAD/EMTDC 소프트웨어를 통한 시뮬레이션과 5kw프로토타입 하드웨어 장치를 통해 제안된 단독 운전 검출 방법을 검증하였다.

• Journal of Microbiology
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• v.35 no.3
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• pp.228-233
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• 1997

The MTF1 gene of Saccharomyces cerevisiae encodes a 43 kDa MITOCHONDRIAL RNA polymerase specificity factor which recognizes mitochondrial promoters to initiate correct transcription. To better understand structure-function of the MTF1 gene as well as the transcription mechanism of mitochondrial RNA polymerase, two cold-sensitive alleles of the MTF1 mutation were isolated by plasmid shuffling method after PCR-based random mutagenesis of the MTF1 gene. The mutation sites were analyzed by nucleotide sequencing. These cs phenotype mtf1 mutants were respiration competent on the nonfermentible glycerol medium at the permissive temperature, but incompetent at 13.deg.C. The cs phenotype allele of the MTF1, yJH147, encoded an L146P replacement. The other cs allele, yJH148, contained K179E and K214M double replacements. Mutations in both alleles were in a region of Mtflp which is located between domains with amino acid sequence similarities to conserved regions 2 and 3 of bacterial s factors.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.66.2-66
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• 2001

Double-effect absorption chiller-heaters have been widely installed in large buildings and are also becoming popular in mid-sized commercial buildings. While much emphasis is Put on designing energy saving and efficient chiller-heaters, careful attention must also be given to the design of control systems for these devices. Currently, these devices are controlled by their own built-in controllers, such as relay based sequence controller or microprocessor based controller. But, because large buildings require multiple chiller-heaters, a centralized supervision and control system is necessary for the efficient monitoring, control, and operations management of these devices We have developed man-machine interface software that allows the implementation of such a system. This system will support increased reliability, more efficient building management operations, and reduced ...