• Korean Journal of Animal Reproduction
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• v.17 no.3
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• pp.249-255
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• 1993

Canine follicular oocytes were used to establish a reliable system for maturation and fertilization in vitro. Ovaries were obtained from either slaughter house or hormone-primed bitches of mixed breeds. The oocytes were recovered by mincing the ovaries in M2＋BSA. Good quality of oocyte-cumulus complexes (OCCs) were selected and cultured in TCM 199 containing 15% fetal calf serum(FCS) for 24~56 h in an atmosphere of 5% CO2 at 39$^{\circ}C$. Maturation rate of follicular oocytes was >87% showing metaphase I. Unlike other domestic animals the cumulus expansion did not occur fully in canine OCCs although minimum expansion was found between the cumulus cells and corona radiata cells, the clear nuclear morphology was presented for the first time by rapid staining. The IVM system used in this study may be useful to obtain fully maturated metaphase I oocyte in dog.

• Korean Journal of Veterinary Research
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• v.1 no.1
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• pp.65-70
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• 1961

Many losses in domestic animals have bern reported in this country, since sodium monofluoroacetate (Compound 1080) was used as a rodenticide. Lesions described inclubed multiple petechiae in epi- and endocardium, lung and intestinal mucosa, or superficial necrosis of the gastric mucosa. In this studies as to the poisoning 7 rabbits were administered 0.6mg (Group I), 0.4mg (Group II), and 0.3mg (Group III) of sodium monofluoroacetate per kilogram of body weight. and the results obtained were as follows: In addition to the changes mentioned above fatty degeneration in central parts of hepatic lobule or nut meg liver, haemorrhagic feci in cerebral cortex and leptomeninges and fatty degeneration in kidney and cardiac muscles were found.

• Proceedings of the KIEE Conference
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• 2002.06a
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• pp.153-157
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• 2002

Electricity living body formation operation appliance is using most imports until present. And, because electricity living body formation operation appliance is too expensive, it becomes burden to buy in small scale hospital. Also, problem that economical damage by income is added follows, Therefore, this treatise developed electricity living body formation operation appliance by home production, Ultra high frequency eruption circuit used LC circuit, and output frequency is possible to 8 [MHz], and output voltage amplified maximum 800 [V]. This operation appliance could display size of fixed current. This developed product could microscopic operation fewer than 1 [mm]. This development technology is considered by do metallic surface heat treatment, melting, welding, induction heating device and that can apply to domestic animals surgical operation and so on.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2002.05a
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• pp.127-132
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• 2002

Electricity living body formation operation appliance is using most imports until present. And, because electricity living body formation operation appliance is too expensive, it becomes burden to buy in small scale hospital. Also, problem that economical damage by income is added follows. Therefore, this treatise developed electricity living body formation operation appliance by home production. Ultra high frequency eruption circuit used LC circuit. and output frequency is possible to 8 [MHz], and output voltage amplified maximum 800 [V]. This operation appliance could display size of fixed current. This developed product could microscopic operation fewer than 1 [mm]. This development technology is considered by do metallic surface heat treatment, melting, welding, induction heating device and that can apply to domestic animals surgical operation and so on.

• Journal of the Korean Professional Engineers Association
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• v.33 no.3
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• pp.30-34
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• 2000

In recent years, disputes are on the increase that noise and vibration by construction equipments and blasting do damage to livestock farming families around construction sites in the construction of a road or a railway. Therefore, the following ways of improvement were presented in solving the existing problems of livestock disputes. $.$ To minimize the damage of livestock farming families by effect assessment of noise and vibration in the region expected to be damaged before the start of construction $.$ To maintain a thorough management system that can reduce noise and vibration during construction work $.$ To apply an adequate measurement method of noise and vibration taking field conditions into consideration

• Parasites, Hosts and Diseases
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• v.45 no.4
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• pp.267-271
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• 2007

Theileria annulata, a protozoan parasite of cattle and domestic buffaloes, is transmitted by ticks of the genus Hyalomma, and causes a disease named Mediterranean or tropical theileriosis. In this research 50 cattle naturally infected with Theileria annulata were treated with the extract of the plant Peganum harmala. The treatment was continued for 5 days, the dose of the extract being 5 mg/kg per day. After the treatment, 39 cattle responded to the treatment and recovered, but 11 did not respond to the treatment and died. The recovery rate of animals treated with the extract of the plant Peganum harmala was 78%.

• Proceedings of the KSAR Conference
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• 2001.10a
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• pp.48-49
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• 2001

While transgenic manipulation in mice have been very successful the same is not true for cattle and pigs. The inability to isolate ES cells from the bovine and porcine has precluded the utilization of the gene targeting technology in these species. Fortunately new advances in cloning by nuclear transfer have opened up a unique opportunity to undertake precise genetic modification in cattle and pigs. The ability of a number of different laboratory groups to successfully clone cattle is due to numerous research programs focused on nuclear transfer in cattle, and the enormous base of knowledge developed over the last 20 years involving the application of assisted reproductive techniques in cattle. Successful and repeatable procedures for in vitro oocyte maturation, in vitro fertilization, and in vitro embryo culture are now well established for cattle. In our laboratory we have utilized nuclear transfer to reproduce the genotypes of several animals, selected for cloning based on their inherent genetic value. Results that we have obtained to date are similar to those reported by other laboratories. (omitted)

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.11
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• pp.1631-1635
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• 2007

Japanese Brown cattle, one of the four domestic beef breeds in Japan, are suffering from numerical reduction due to economic pressure from profitable breeds. In this study, all the reproductive cows in the Kouchi sub-breed of the Japanese Brown cattle that were alive in July 2005 were investigated by pedigree analysis to clarify genetic structure and composition of genetic variability. In addition, genetically important individuals for the maintenance of genetic variability of the sub-breed were also identified through the core set method. The number of cows analyzed was 1,349. Their pedigrees were traced back to ancestors born around 1940, and pedigree records of 13,157 animals were used for the analysis. Principal component analysis was performed on the relationship matrix of the cows, and their factor loadings were plotted on a three-dimensional diagram. According to their spatial positions in the diagram, all the cows were subdivided into five genetically distinctive subpopulations of 131 to 437 animals. Genetic diversity of the whole sub-breed, which is estimated to be 0.901, was decomposed into 0.856 and 0.045 of within-subpopulation and between-subpopulation components. Recalculation of genetic diversity after removal of one or several subpopulations from the five subpopulations suggested that three of them were genetically important for the maintenance of genetic variability of the sub-breed. Applying the core set method to all the cows, maximum attainable genetic diversity was estimated to be 0.949, and optimal genetic contributions assigned to each cow supported the previous results indicating relative importance of the three subpopulations as useful genetic materials.

• Applied Microscopy
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• v.14 no.2
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• pp.66-80
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• 1984

The organic phosphorus compounds have been widely used as an insecticide, since toxicity of these compounds is especially drastic to the insects than to men and other mammals. The organic phosphates are rapidly hydrolized and hence have little cumulative and ecologic effects. However, due to their acute toxic effects organophosphate have recorded rather high fatalities in men and domestic animals. The organic phosphorus compounds are powerful inhibitors to the carboxylic esterase enzymes such as acetylcholinesterase and pseudocholinesterase. As a result of firm binding characteristics of phosphate radicals to the active sites of enzyme, the activities of these enzymes are inhibited by the organophosphates. The organophosphates such as diazinon is easily observed from skin, gastrointestinal tract, conjunctivas and respiratory tract, and it is converted to more toxic form during metabolism in the liver The present study was carried out in order to investigate the hepatotoxicity of diazinon by observing the changes in the ultrastructure of cytoplasmic organelles of hepatic cells in albino mice. The animals were killed at 6, 12 and 24 hours after administration of 25mg/kg diazinon. The piece of hepatic tissue obtained from each animal was ultrathinly sectioned. The specimens stained by uranyl acetate and lead citrate double contrast methods were observed with JEM model 100B electron microscope. The results obtained were as follows: 1) A prominent dilatation and sacculation of the cisternae of rough endoplasmic reticulum associated with detachment of membrane bound-ribosomes, and disaggregation of the free ribosomes were recognized. 2) The hypertrophy of the smooth endoplasmic reticulum associated with depletion of the glycogen particles was observed. 3) The atrophy of cisternae of Golgi complex was observed. 4) A large number of secondary lysosomes (autophagic vacuoles and residual bodies) were formed. Consequently it is suggested that diazinon would induce disorganization of the cytoplasmic organelles of hepatocytes in albino mice.

• Korean Journal of Veterinary Research
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• v.54 no.4
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• pp.197-201
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• 2014

In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.