• 농약과학회지
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• 제14권1호
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• pp.16-20
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• 2010

Thiosultap은 중국에서 사용되고 있는 nereistoxin계 살충제로 국내에는 등록되지 않아 중국산 농산물 수입시 잔류 농약 안전관리를 위한 시험법 마련이 시급한 실정이다. 이에 본 연구에서는 현미 중 thiosultap을 염기조건에서 nereistoxin으로 유도체화하여 GC-FPD로 분석하는 시험법을 개발하였다. 산염기 분배를 이용한 정제 조건을 확립하였으며, 확립된 시험법에 의한 정량한계와 직선성을 측정한 결과 각각 0.05 mg $kg^{-1}$과 0.995$(r^2)$이었다. 무처리 현미에 thiosultap 0.5와 2.5 mg $kg^{-1}$을 처리하여 회수율을 측정한 결과는 $96.1{\pm}7.9\sim100.8{\pm}6.1%$로 양호한 결과를 나타내었다.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권3호
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• pp.210-218
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• 2021

Recently, pest-resistant living modified (LM) crops developed using RNA interference (RNAi) technology have been imported into South Korea. However, the potential adverse effects of unintentionally released RNAi-based LM crops on non-target species have not yet been reported. Coccinella septempunctata, which feeds on aphids, is an important natural enemy insect which can be exposed to the double-stranded RNA (dsRNA) produced by RNAi-based LM plants. To assess the risk of ingestion of Snf7 dsRNA by C. septempunctata, we first identified the species through morphological analysis of collected insects. A method for species identification at the gene level was developed using a specific C. septempunctata 12S rRNA. Furthermore, an experimental model was devised to assess the risk of Snf7 dsRNA ingestion in C. septempunctata. Snf7 dsRNA was mass-purified using an effective dsRNA synthesis method and its presence in C. septempunctata was confirmed after treatment with purified Snf7 dsRNA. Finally, the survival rate, development time, and dry weight of Snf7 dsRNA-treated C. septempunctata were compared with those of GFP and vATPase A dsRNA control treatments, and no risk was found. This study illustrates an effective Snf7 dsRNA synthesis method, as well as a high-concentration domestic insect risk assessment method which uses dsRNA to assess the risk of unintentional released of LM organisms against non-target species.

• 한국작물학회지
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• 제56권4호
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• pp.308-314
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• 2011

녹비종자의 입모증진 및 지속재배 가능성을 구명하고자 국내산 녹비종자의 종자의 성숙시기별 발아 및 휴면 특성과 수입녹비종자의 종자활력유지기간을 구명한 결과는 다음과 같다. 1. 녹비종자의 채종시기별 발아율은 5월 25일에서 6월 4일로 종자 성숙이 진전 될수록 경실율이 높아져 낮아졌다. 6월 4일 수확한 녹비종자의 무처리 발아율은 마메초 30%, 크림손클로버 16%, 살갈퀴는 2%로 낮았으나 종피 파상처리후 종자활력은 마메초 85%, 크림손 클로버 94%, 살갈퀴는 95%로 높아 녹비종자의 휴면은 종자경실에 기인한 것으로 확인되었다. 2. 녹비종자 성숙시기별 종자 활력은 크림손클로버와 살갈퀴는 5월 30일 이후에는 90%이상으로 높았으나 마메초는 77~85%로 낮아 크림손클로버와 살갈퀴는 밀양에서 5월 30이후 완전히 성숙이 되었으나 마메초는 6월 4일까지도 성숙이 되지 않았다. 3. 6월초에 수확한 녹비종자를 상온저장 후 매월 발아율 및 경실율(휴면율)을 조사한 결과, 저장기간이 경과함에 따라 경실종자의 비율이 감소하여 발아율이 증가하였으나 녹비종자 파종시기인 9월까지도 발아율이 26~34%로 낮았다. 따라서 국내 생산 녹비종자 이용시 9월 파종직전 휴면타파 처리를 해야 안전 입모수 확보가 가능할 것으로 판단되었다. 4, 수입 녹비종자의 광조건이 암조건보다 발아율이 4~5% 정도 높았으나 통계적인 유의성은 없어 녹비종자의 발아에 광의 영향은 크게 받지 않았다. 5. 수입 헤어리베치와 크림손클로버 종자를 상온보관시 1년후에는 발아율이 헤어리베치는 18∼32%, 크림손 크로버는 30~40%가 낮았고, 2년후에는 발아율이 헤어리베치는 5~13% 정도로 낮고 크림손클로버는 전혀발아가 되지 않아 수입후 2년이 경과한 종자는 사용하지 말아야 한다.

• 유통과학연구
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• 제8권3호
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• pp.17-26
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• 2010

세계경제 및 환경의 변화에 따라 농산물의 불안정적인 수급으로 인한 문제점이 노출되고 있으며, 우리나라의 경우 농산물의 안정적인 확보를 위하여 국가 전략적 차원에서의 해외농업투자의 필요성이 요구되고 있다. 하지만 정부차원의 지원 미진, 해외 농업에 대한 정보 및 기술 미비, 개발자금 확보의 어려움, 장기간의 투자금 회수기간, 사후관리 미흡 등의 이유로 성과를 이루지 못하고 있는 실정이며, 특히 해외 농산물의 국내 반입 시 관세의 장벽, 물류 유통비용 등으로 가격 경쟁력이 떨어지고 있어 국내에 반입되지 못하고 있는 실정이다. 이에 본 연구에서는 우리나라의 해외농업투자의 기본개념 및 실태를 살펴보고 해외농업투자의 필요성과 고려사항, 문제점 등을 도출하여 해외에서 재배된 농산물의 경쟁력을 위한 유통 측면에서의 개선방안을 정부의 간접적인 지원, 유통 현대화 및 유통정보기능 강화, 유통시설, 수송루트, 하역업무개선, 경쟁력 확보를 위한 정부의 정책적 지원, 교육 훈련을 통한 전문인력 양성 등 다섯 가지 측면에서 제시하였다.

• 생명과학회지
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• 제16권5호
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• pp.806-811
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• 2006

The regulation of labelling criterion for genetically modified (GM) foods has been enforced since 2001 in Korea. Therefore, GM soybean (GMS) or GM maize (GMM) processed foods must be labeled as GMO derived. We surveyed to see whether this regulation is kept relevantly or not and the distributive statue of GM processed foods. Using the method of polymerase chain reaction (PCR) based on endogenous gene (Le1n, SSIIb), promoter gene (P35S), terminator gene (NOS) and transgenic gene (RRS, Bt11, Bt176, GA21, T25, Mon810), we detected GMS and GMM processed foods circulating at the market in Busan area. Out of total 100 samples, 38 items were showed to be contaminated with recombinant gene by qualitative PCR. Among 82 domestic and 18 imported items, 32 (39.0%) and 6 (33.3%) items were detected with GM ingredients respectively. Also among the 80 soybean and 20 maize processed foods, 23 (28.7%) and 15 (75.0%) foods were sensitive to detect GMS and GMM ingredients respectively. For the qualitative PCR positive foods, we chased identity preservation (IP) certificates. And we verified that the PCR positive crops were grown up, harvested and shipped separately from GMO but just mixed with GMO in the threshold of the non attentional contamination levels (3%). Thus we can not find out any regulation-violent case at all. The results of this study will help to keep the regulations of GM labelling and be informative to consumers who want to know the laboratory results of GMO testing.

• 농업과학연구
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• 제38권3호
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• pp.445-452
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• 2011

The object of this experiment was to investigate the food habits of Korean native goats fed with various roughage sources. The experimental trials were conducted at Unbong Animal Genetic Resources Station in 2008. The experimental roughages include five sources and 25 species in all; grasses and legumes: 5 species (mixed grasses, orchardgrass, tall fescue, alfalfa, white clover), native grasses and weeds: 5 species (mixed native grasses, Miscanthus sinensis Anderss, Arundinella hirta (Thunb.) Tanaka, Barnyard grass, short awn, forage crops and straw: 5 species (barley + hairy vetch, wheat + hairy vetch, rye silage, barley silage, baled rice straw), browse and fallen leaves: 5 species (Mixed browse, Oriental white oak, Quercus serrta Thunb., Oriental cherry fallen leaves, Japanese chestnut fallen leaves), and imported hay and straw: 5 species(timothy hay, tall fescue straw, annual ryegrass straw, klinegrass hay, alfalfa hay). Ten Korean native goats were selected which had nearly the same body weight (average $24{\pm}2.8$ kg). The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period (p<0.05). Among all the 25 species of roughages, the favorite intake species order by Korean native goat was observed like this: Quercus aliena, Querancas serrta Thunb and Mixed browse, which was a lower intake compared to other domestic herbivores. The Korean native goats ate more roughages which had low fibrous contents, but high dry matter digestibility. The Korean native goats ate more roughages which had low fibrous contents, but high dry matter digestibility. On the other hand, compared to each roughage source, total intake amount by Korean native goats was showed as browse and fallen leaves (33.1%) among trials roughage sources. Based on these results, the food habit of Korean native goats seems to be closer to the browser.

• 한국작물학회지
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• 제39권2호
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• pp.198-204
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• 1994

농업에 관한 우리나라 전문 고농서는 조선조에 많이 발간되어 중·근세 농업문화를 이해하는데 도움이 되었다. 여기서는 고농서, 국내외 문헌 및 자료를 바탕으로 담배의 명칭과 그의 전래를 요약한바 o남영초, 남초, 연초, 어, 연초, 서초, 왜초, 호초, 담파고등으로 기록되고 o국내문헌은 무오년(1618년)을 전후한 년대에 일본 전래설을 통설로 기술되어 있으나 o 일본이 과거 중국, 한국과의 문화교류와 내량, 평안시대에 많은 작물을 도입재배한 역사적 실증과 o한국에의 전래근거가 없다는 인접국(중국, 일본)의 이설 o우리나라 담배 산지 재래종 기원항설과 o김대현(1553-1612년)지방거주와 유정과의 서신사기, 구전등으로 보아 o임진왜란 중 (1592-98년) 명나라 유정장군의 병사에 의한 중국 전래설이 가장 신빙성이 있다고 하겠다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.345-345
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• 2017

In order to reduce rice cultivation area in paddy fields and to increase domestic self-sufficiency of imported upland grain, it is necessary to increase double cropping area of upland crops in paddy field in accordance with decrease of rice consumption in Korea. The double cropping of spring potato-summer grain maize can be combined because of enough growing season in the southern plain of Korea. Spring potato, which is profitable, can be planted in the late February and harvested in the late May as the main crop. Subsequent grain maize can be planted in early June and harvested in November (maturity in the early October). Spring potato (variety Soomi) yielded $2,544kg\;10a^{-1}$ (tuber) when planted in late February, 2016. When maize was planted in June as the second cropping crop, though growth of plant decreased much, grain yield decreased slightly compared to normal planting in April or May. There was enough time to dry maize ear in the field after maturity before harvesting, which saved labor and time for grain drying, since there is no autumn planting at the double cropping of spring potato-summer grain maize. When grain maize (variety Gwangpyeongok) was planted in the early June (June $10^{th}$), average grain yield of above $860kg\;10a^{-1}$ over 2 years of 2015 and 2016 was obtained, and the annual total yield (potato tuber + maize grain) of 3,400 kg $10a^{-1}$ was obtained. The result indicates that the double cropping of spring potato-summer maize using paddy fields in southern plain of Korea, could contribute to the self-sufficiency of upland crops through the maximum production.

• 한국환경농학회지
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• 제31권2호
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• pp.157-163
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• 2012

수입 농식품에 대하여 국내에 등록되지 않아 식품공전 상의 분석법이 미확립된 chlorthal-dimethyl, clomeprop, diflufenican, hexachlorobenzene, picolinafen, propyzamide등 6종의 농약에 대해 GC-ECD로 다성분 동시분석법을 확립하였고, GC/MS로 확인하였다. 대상작물은 현미와 오렌지 2종을 선정하였고, 분석법은 식품의약품안전청에서 고시한 다종농약다성분 동시분석법을 적용하였다. 시료의 추출은 acetonitrile을 사용하였고 Florisil SPE 정제과정을 거쳤다. 분석법의 정량한계는 0.02~0.05 mg/kg 이었고, 직선성은 0.05~10.0 mg/kg 범위에서 $R^2$ >0.999였다. 평균회수율은 정량한계 수준에서 74.4~110.3%, 정량한계 수준의 10배에서 77.8~118.4%였으며, 변이계수는 전체시료에서 8.8% 이하로 잔류농약 분석기준인 회수율 70~120%와 10% 이내의 변이계수 조건을 충족시켰다. 이와 같이 확립된 6종의 다성분 분석법은 수입농산물 분석에 적용이 가능할 것으로 판단된다.

• 한국환경농학회지
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• 제39권3호
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• pp.246-252
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• 2020

BACKGROUND: Pesticides are broadly used to control weeds and pests, and the residues remaining in crops are managed in accordance with the MRLs (maximum residue limits). Therefore, an analytical method is required to quantify the residues, and we conducted a series of analyses to select and validate the quick and simple analytical method for tolpyralate in five agricultural products using QuEChERS (quick, easy, cheap, effective, rugged and safe) method and LC-MS/MS (liquid chromatography-tandem mass spectrometry). METHODS AND RESULTS: The agricultural samples were extracted with acetonitrile followed by addition of anhydrous magnesium sulfate, sodium chloride, disodium hydrogencitrate sesquihydrate and trisodium citrate dihydrate. After shaking and centrifugation, purification was performed with d-SPE (dispersive-solid phase extraction) sorbents. To validate the optimized method, its selectivity, linearity, LOD (limit of detection), LOQ (limit of quantitation), accuracy, repeatability, and reproducibility from the inter-laboratory analyses were considered. LOQ of the analytical method was 0.01 mg/kg at five agricultural products and the linearity of matrix-matched calibration were good at seven concentration levels, from 0.0025 to 0.25 mg/L (R²≥0.9980). Mean recoveries at three spiking levels (n=5) were in the range of 85.2~112.4% with associated relative standard deviation values less than 6.2%, and the coefficient of variation between the two laboratories was also below 13%. All optimized results were validated according to the criteria ranges requested in the Codex Alimentarius Commission (CAC) and Ministry of Food and Drug Safety (MFDS) guidelines. CONCLUSION: In conclusion, we suggest that the selected and validated method could serve as a basic data for detecting tolpyralate residue in imported and domestic agricultural products.