• BMB Reports
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• v.43 no.6
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• pp.407-412
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• 2010

Homeodomain (HD) is a highly conserved DNA-binding domain composed of helix-turn-helix motif. Drosophila Vnd (Ventral nervous system defective) containing HD acts as a regulator to either enhance or suppress gene expression upon binding to its target sequence. In this study, kinetic analysis of Vnd binding to DNA was performed. The result demonstrates that DNA-binding affinity of the recombinant protein containing HD and NK2-specific domain (NK2-SD) was higher than that of the full-length Vnd. To access whether phosphorylation sites within HD and NK2-SD affect the interaction of the protein with the target sequence, alanine substitutions were introduced. The result shows that S631A mutation within NK2-SD does not contribute significantly to the DNA-binding affinity. However, S571A and T600A mutations within HD showed lower affinity for DNA binding. In addition, DNA-binding analysis using embryonic nuclear protein also demonstrates that Vnd interacts with other nuclear proteins, suggesting the existence of Vnd as a complex.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.135-139
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• 2002

Yeast cells respond to condition of amino acid starvation by synthesizing GCN4, a typical eukaryotic transcriptional activator, which regulates the expression of many amino acids biosynthetic genes. By introducing point mutations in the DNA binding domain of GCN4, mutants with normal DNA binding activity but defective in transcriptional activity were isolated to identify unknown proteins that could suppress the mutant phenotype under an amino acid depletion condition. As a result, SSB(Stress-Seventy B) subfamily proteins were identified as suppressors of mutant GCN4. SSB proteins were known as a member of yeast hsp70 family that probably aids passage of nascent chain through ribosomes. Among them, the mechanism of suppression by SSB2 on the defective GCN4 mutant strains is under investigation. Gcn4p directly interacts with Ssb2p through the basic DNA binding domain of GCN4. It suggests the possibility that physical interaction might induce the transcriptional activation of Gcn4p.

• Journal of Microbiology
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• v.34 no.4
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• pp.311-315
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• 1996

The retroviral Gag polyprotein directs the assembly of virion particles and plays an important role in some events after entry into a host cell. The Gag polyprotein of a virus mixture is responsible for inducing murine acquired immunodeficiency syndrome (MAIDS) when injected into susceptible strains of mice. In order to identify the host cellular proteins which interact with the MAIDS virus Gag proteins and possibly mediate the function of the Gag proteins, mouse T-cell leukemic cDNA expression library was screened using the yeast GAL4 two hybrid system. Of 11 individual positive clones, the clone Y1 was selected for the study of protein-protein interaction. Its DNA sequence revealed that it was an exact match to the murine SH3 domain-containing protein SH3P8. It is expressed as 2.4 kbp transcripts in testis at higher levels and in various tissues tested at lower levels. Glutathione S-transferase-Y1 fusion protein binds tightly to $Pr60^{def-gag}$ as well as $Pr65^{eco-gag}$.

• The Transactions of the Korean Institute of Power Electronics
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• v.3 no.4
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• pp.338-345
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• 1998

The mathematical interpretation of a practical sampler which is useful to obtain the small signal models for the peak and average current mode controls is proposed. Due to the difficulties in applying the Shannons sampling theorem to the analysis of sampling effects embedded in the current mode control, several different approaches have been reported. However, these approaches require the information of the inductor current in a discrete expression, which restricts the application of the reported method only to the peak current mode control. In this paper, the mathematical expressions of sampling effects on a current loop which can directly apply the Shannons sampling theorem are newly proposed, and applied to the modeling of the peak current mode control. By the newly derived models of a practial smapler, the models in a discrete time domain and a continuous time domain are obtained. It is expected that the derived models are useful for the control loop design of power supplies. The effectiveness of the derived models are verified through the simulation and experimental results.

• Proceedings of the KIPE Conference
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• 1998.07a
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• pp.275-278
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• 1998

The mathematical interpretation of a practical sampler which is useful to obtain the small signal models for the peak and average current mode controls is proposed. Due to the difficulties in applying the Shannon's sampling theorem to the analysis of sampling effects embedded in the current mode control, several different approaches have been reported. However, these approaches require the information of the inductor current in a discrete expression, which restricts the application of the reported method only to the peak current mode control. In this paper, the mathematical expressions of sampling effects on a current loop which can directly apply the Shannon's sampling theorem are newly proposed, and applied to the modeling of the peak current mode control. By the newly derived models of a practical sampler, the models in a discrete time domain and a continuous time domain are obtained. It is expected that the derived models are useful for the control loop design of power supplies. The effectiveness of the derived models are verified through the simulation and experimental results.

• Journal of Microbiology and Biotechnology
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• v.20 no.11
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• pp.1484-1490
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• 2010

In the Streptomyces hygroscopicus JCM4427 geldanamycin biosynthetic gene cluster, five putative regulatory genes were identified by protein homology searching. Among those genes, gel14, gel17, and gel19 are located downstream of polyketide synthase genes. Gel14 and Gel17 are members of the LAL family of transcriptional regulators, including an ATP/GTP-binding domain at the N-terminus and a DNA-binding helix-turn-helix domain at the C-terminus. Gel19 is a member of the TetR family of transcriptional regulators, which generally act to repress transcription. To verify the biological significance of the putative regulators in geldanamycin production, they were individually characterized by gene disruption, genetic complementation, and transcriptional analyses. All three genes were confirmed as positive regulators of geldanamycin production. Specifically, Gel17 and Gel19 are required for gel14 as well as gelA gene expression.

• Journal of The Korean Astronomical Society
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• v.27 no.2
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• pp.191-201
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• 1994

We propose to use the entropy of power spectra defined in the frequency domain for the deconvolution of extended images. Spatial correlations requisite for extended sources may be insured by increasing the role of power entropy because the power is just a representation of spatial correlations in the frequency domain. We have derived a semi-analytical solution which is found to severely reduce computing time compared with other iteration schemes. Even though the solution is very similar to the well-known Wiener filter, the regularizingng term in the new expression is so insensitive to the noise characteristics as to assure a stable solution. Applications have been made to the IRAS $60{\mu}m\;and\;100{\mu}m$ images of the dark cloud B34 and the optical CCD image of a solar active region containing a circular sunspot and a small pore.

• Lingua Humanitatis
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• v.1 no.1
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• pp.221-233
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• 2001

The purpose of this paper is twofold. On the one hand it takes issue with Engstrom's claim that conceptual metaphors are propositional; on the other, it aims to demonstrate that the mathematical term 'mapping' is inappropriate for the analysis of metaphors. To my mind, the propositional analysis of metaphors, a wrong analysis for that matter, originates in the notion 'mapping' I argue that partial 'mapping' between propositional meanings and metaphorical meanings is either mental or psychological, with no concomitant 'truth' value. When concept metaphors represent propositionality, they lose metaphoricity; when they obtain metaphoricity, they are free of propositionality. The mathematical terms 'mapping' and 'proposition,' it is stressed, should be avoided in the analysis of concept metaphors like 'A is B' because they are confusing when applied to linguistic expression. 1 suggest that the term 'mapping' be replaced by phrases such as 'interaction between two domains,' projection from source-domain to target domain,' or 'understanding the properties of two domains between A and B,' etc. This would amount to proposing a pragmatic or cognitive theory of metaphor.

• BMB Reports
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• v.47 no.7
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• pp.405-410
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• 2014

ZBTB3 belongs to the Zinc finger and BTB/POZ domain containing transcription factor family; however, its biological role has rarely been studied. We demonstrate for the first time, to our knowledge, that ZBTB3 is an essential factor for cancer cell growth via the regulation of the ROS detoxification pathway. Suppression of ZBTB3 using two different short hairpin RNAs in human melanoma, lung carcinoma, and breast carcinoma results in diminished cell growth. In addition, we found that suppression of ZBTB3 activates a caspase cascade, including caspase-9, -3, and PARP leading to cellular apoptosis, resulting from failed ROS detoxification. We identified that ZBTB3 plays an important role in the gene expression of ROS detoxification enzymes. Our results reveal that ZBTB3 may play a critical role in cancer cell growth via the ROS detoxification system. Therefore, therapeutic strategies that target ZBTB3 could be used in selective cancer treatments.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.87-93
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• 2004

Dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) on Ser and Thr residues is ubiquitous in higher eukaryotes. And this modification may serve as a signaling mod-ification analogous to protein phosphorylation. Addition and cleavage of O-GlcNAc are catalyzed by O-linked GlcNAc transferase (OGT) and O-linked N-acety1glucosaminidase (O-GlcNAcase), respectively. Two types of human O-GlcNAcase gene were cloned and expressed as three fusion proteins in Escherichia coli. O-GlcNA-case activity showed in the order of thioredoxin fusion> $6{\times}His$ tag> GST fusion. O-GlcNAcase had enzy-matic activity against only ${\rho}$NP-GlcNAc of seven tested substrate analogs. Blast search revealed that O-GlcNAcase has two conserved domains, amino terminal hyaluronidase-like domain and carboxy terminal N-acetyltransferase domain. Extensive deletion studies were done to define catalytically important domains. The deletions of hyaluronidase-like domain and N-acetyltransferase domain abolished enzyme activity. But, N-ter-minal 55 amino acid deletion and C-terminal truncation showed lower activity. Based on deletion analysis, we suggest that hyaluronidase-like domain is essential for enzyme activity and carboxy terminal N-acetyltrans-ferase domain may be modulatory function.