• Title/Summary/Keyword: docosanoic

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The Constituents of the Aerial Part of Gastrodia elata Blume

  • Liu, Xiang Qian;Baek, Wan-Sook;Ahn, Duk-Kyun;Choi, Ho-Young;Yook, Chang-Soo
    • Natural Product Sciences
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    • v.8 no.4
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    • pp.137-140
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    • 2002
  • From the MeOH extract of the aerial part of Gastrodia elata Blume (GEB) (Family: Orchidaceae), eight compounds have been isolated on repeated column chromatography, and their structures were elucidated as dotriacontanoic acid (1), beta-sitosterol (2), 4-hydroxybenzaldehyde (3), docosanoic acid oxiranylmethyl ester (4), hentriacotanoic acid (5), octadecanoic acid (6), benzoic acid (7) and gastrodin (8) on the basis of their spectral evidences including EI-Mass and 2D-NMR spectrum. All of them were obtained from the aerial part of Gastrodia elata Blume for the first time, in which compound 4 is a novelty to our best knowledge. It is also known that a phenolic glucoside, gastrodin is a major constituent just like Gastrodia rhizome.

Studies on the Preparation of Durable Softners using Alkyl Imidazoline and Fatty Carbamide (알킬이미다졸린과 지방산 카르바미드를 이용한 내구성 유연제의 제조에 관한 연구)

  • Jung, Choong-Ho;Park, Hong-Soo;Kim, Young-Keun
    • Applied Chemistry for Engineering
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    • v.4 no.1
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    • pp.54-63
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    • 1993
  • Some emulsified 1, 2-disubstituted imidazolinium salts were synthesized by quaternization of 1, 2-disubstituted imidazoline which had been prepared by the reaction of dodecanoic acid with tetraethylenepentamine. 2-Docosamido-2'-docosanoyloxydiethylamine was prepared by the reaction of docosanoic acid with hydroxylethylethylenediamine. Then, some emulsified fatty carbamide salts were also synthesized by quaternization of fatty carbamide which had been prepared by the reaction of urea with the 2-docosamido-2'-docosanoyloxydiethylamine. Some softners were prepared by blending of these salts mentioned above. These softners were treated on acrylic fiber, then several properties were measured. As the results, softening and lubricating properties are good and a little antistatic property was observed. The synthesized softners were also proved as a durable softner by means of the several experiments : bending resistance test and measurements of feeling change with washing according to the pH changes. The surface structure of acrylic fibers treated with the softner were characterized by SEM.

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Chemical Constituents and Biological Activity of Kalopanacis Cortex (해동피의 화학성분 및 생리활성)

  • Lee, Eun;Choi, Moo-Young;Park, Hee-Juhn;Cha, Bae-Chun;Cho, Soon-Hyun
    • Korean Journal of Pharmacognosy
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    • v.26 no.2
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    • pp.122-129
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    • 1995
  • The study on phytochemical analysis and the biological activity of Kalopanacis Cortex was carried out in this research. As a phytochemical result, liriodendrin as a lignan glycoside was isolated and characterized. Two subfraction separated from the acidic substance of $CHCl_3$ fraction were saturated and unsaturated fatty acid, respectively. Saturated fatty acid mixture identified from GC-MS tool was as follows: palmitic acid, stearic acid, arachidic acid, heneicosanoic acid, docosanoic acid, tricosanoic acid, tetracosanoic acid, pentacosanoic acid, hexacosanoic acid and octacosanoic acid. Unsaturated fatty acid was found to be linoleic acid on the basis of spectroscopic method. An active principle of liriodendrin exhibited significant antihepatotoxic activity but failed to show a considerable antiedemic activity. In this paper, the result of writhing test on liriodendrin was also described.

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Studies on the Chemical Components and Antioxidative Effect of Solanum lyratum Thunb (배풍등의 화학성분 및 항산화 효과에 관한 연구)

  • Shim, Kyung-Hee;Young, Han-Suk;Lee, Tae-Woong;Choi, Jae-Sue
    • Korean Journal of Pharmacognosy
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    • v.26 no.2
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    • pp.130-138
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    • 1995
  • Phytochemical study on the aerial parts of Solanum lyratum (Solanaceae) was carried out. On the basis of phytochemical and spectroscopic evidences, compound I was identified as mixtures of hexadecanoic acid methyl ester, 2,6,10,15-tetramethyl heptadecane, tricosane, tetracosane, pentacosane, docosanoic acid methyl ester, docosane, tricosanoic acid methyl ester, 8-hexyl pentadecane, tetracosanoic acid methyl ester, pentatriacontane, hexatriacontane, eicosane, hexacosane, hentriacontane and stigmasta-5,23-dien $3-{\beta}-ol$, and compound II, III, IV and V were identified as hexacosanoic acid methyl ester, ${\beta}-sitosterol-{\beta}-{_D}-glucoside$, $3-O-{\alpha}-{_L}-rhamnopyranosyl-(1->2)-{\beta}-{_D}-glucuronopyranosyl$ diosgenin and $3-O-{\alpha}-{_L}-rhamnopyranosyl-(1->6)-{\beta}-{_D}-glucopyranosyl$ quercetin (rutin), respectively. Rutin was identified as one of the active principles having antioxidative effect from S. lyratum.

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The Scientific Analysis of the Archaeological Soil excavated at bunto-ri, Haenam and kumjang-ri, Kyungju (유적지에서 출토된 고대 토양의 과학적 분석연구-해남 분토리 및 경주 금장리 유적지 토양을 중심으로)

  • Seo, Min-seok;Kim, Min-hee
    • 보존과학연구
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    • s.27
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    • pp.203-214
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    • 2006
  • The work focuses on the chemical analysis of organic residues in archaeological soils. Particularly, the detection of manuring in archaeological soils can provide important information concerning early human behavior, diet, parasites, ecological adeptation. In this study, archaeological soils excavated at bunto-ri, Haenam and kumjang-ri, Kyungju were used to assess the possibility as indicators of ancient human activity in archaeological areas. The sampled soils were analyzed soil color, pH for their physical and chemical properties and GC/MSD to detect and quantify specific compound. The results showed that the sampled soils were normal pH(6.8~7.2) and soil color of light brown to yellowish brown. Also, the result from the GC/MS analysis indicated that their compounds were hexadecanoic acid, octadecanoic acid, nonadecane, docosanoic acid, methyl ester, teracosanoic acidand methyl este from bunto-ri site and 1-heptadecene, cyclotetracosane, tetracosane, cyclotetracosane,1-docosene, n-nonadecane, tetracosanoic acid methyl ester, cyclooctacosane, 1-nonadecene, eicosane, cyclotriacontane from kumjang-ri site. These compounds are not only normal soil materials but also animal lipid compounds. And the more scientific analysis of the soils will solve a curiosity for artificially incoming.

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An Analytical Method of Thromboxane $B_2$ by Fast Atom Bombardment Mass Spectrometry (고속원자충격질량분석법을 이용한 Thromboxane $B_2$ 분석)

  • Jang, Suk-Yoon;Kim, Jung-Hoon;Lee, Yong-Moon;Jang, Seung-Ki;Moon, Dong-Cheul
    • Analytical Science and Technology
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    • v.6 no.4
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    • pp.349-357
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    • 1993
  • Analytical methods of thromboxane $B_2(TXB_2)$ using various techniques of Fast Atom Bombardment mass spectrometry (FAB MS) were studied, static FAB condition was investigated to obtain linear response curve using docosanoic acid as a internal standard. For maximum sensitivity, a continuos-flow(CF-) FAB MS by selected-ion monitoring(SIM) with devised sample introduction system, has been developed to quantiate thromboxane $B_2$ in biological sample. Instrumental parameters affecting sensitivity, reproducibility has been studied. The method has been optimized with respect to the eluent, 0.75% glycerol(in EtOH v/v) and flow rate of $3.7{\mu}l/min.$ Under the condition, detection limits were below 10pg in SIM mode and a good linear relationship between dose and response was achieved.

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Simultaneous Determination of C22-C26 Very Long - Chain Fatty Acids Following tert-Butyldimethylsilyl Derivatization by Stable Isotope GC- MS for the Screening of Adrenoleucodystrophy

  • Yoon, Hye-Ran
    • Biomolecules & Therapeutics
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    • v.15 no.4
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    • pp.266-272
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    • 2007
  • A rapid analytical method was developed to quantify very long-chain fatty acids (VLCFAs, C22:0, C24:0, C26:0) in human plasma with good sensitivity and specificity using tert-butyldimethylsilyl (TBDMS) derivatization and stable isotope GC-MS selective ion monitoring (GC-MS/SIM). Two-hundred and fifty ${\mu}L$ of plasma was fortified with deuterated stable isotope internal standards (d3-C22:0, d3-C24:0, d3-C26:0) and standard mixtures of chloroform and methanol, and then extracted with hexane and acetonitrile. To upper layer of liquid-liquid-extraction, N-(t-Butyldimethylsilyl)-N-methyltrifluoroacetamide was added and then heated to $60^{\circ}C$ for 30 min to produce the TBDMS derivatives. Derivatives of VLCFAs were analyzed by GC-MS/SIM. Calibration curves showed a linear relationship for the target compounds in the concentration range of $10^{-4}{\sim}2{\times}10^3\;{\mu}g/mL$ with the correlation coefficient ranging from 0.996 to 0.999. The limit of quantification for the plasma was $10^{-4}{\sim}2{\times}10^{-4}\;{\mu}g/mL$ (S/N=3). When applied to the plasma specimens of patients with peroxisomal disorder, X-linked adrenoleucodystropy (ALD, Mckusick 202370), the method clearly differentiated normal subjects from ALD patients. The C24:0/C22:0 and C26:0/C22:0 ratios were significantly elevated in the plasma of patients with X-linked ALD compared to normal subjects. The new developed method might be useful for a rapid and sensitive diagnosis of X-linked ALD and other peroxisomal disorders.

Physicochemical Comparison of Two Different Shark Meats Used for Preparation of Dombaeki (돔배기용 상어육의 이화학적 성분 비교)

  • Kim, Eun-Ok;Yu, Myeong-Hwa;Lee, Ki-Teak;Kim, Seon-Bong;Choi, Sang-Won
    • Food Science and Preservation
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    • v.15 no.5
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    • pp.711-718
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    • 2008
  • Dombaeki, a traditional salted shark meat, has been widely used as a customary religious food in the Daegu-Gyeongbuk area of Korea. Two different sharks, Sphyrna zygaena (SZ) and Isurus oxyrinchus (IO) are traditionally used to prepare Dombaeki. Chemical components, lipid classes, fatty acid levels, and sterol compositions of meats prepared from the two sharks were investigated. There were no significant differences in chemical composition between the two dried shark meats. Major amino acids in shark meat were leucine, lysine, arginine, proline, isoleucine, valine, phenylalanine, glutamic acid, and asparagine, which together accounted for about 40% of total amino acids. Levels of amino acids in IO meat were higher than in SZ meat. Major fatty acids in the two shark meats were palmitic ($C_{16:0}$), stearic ($C_{18:0}$), oleic ($C_{18:1}$), arachidonic ($C_{20:4}$), and docosahexanoic (DHA, $C_{22:6}$) acids, which accounted for about 80% of total fatty acids. Notably, IO meat showed higher amounts of DHA (31.8%) and eicosapentanoic acid (EPA, 1.8%) than did SZ meat. The two shark meats showed similar dry weight levels of total lipids, with triacylglycerols, free fatty acids, sterols, and phospholipidscomprising on average 5.0, 2.0, 13.0 and 63.0% of total lipids, respectively. The dominant classes of phospholipids were mainly phosphatidylcholine (PC) and phosphatidylethanolamine (PE). PC content in the two shark meats was higher than that of PE, although the differencewas not great. The major fatty acids in phospholipids were myristic, palmitoleic, stearic, and docosanoic acids. Total volatile basic nitrogen content and the pH of SZ meat were lower than those of IO meat, whereas the Hunter's 'a' and 'b' values of SZ meat were higher than those of IO meat. These results suggest that shark meat may be useful as a functional food to prevent several degenerative diseases.