• 한국환경보건학회지
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• 제42권4호
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• pp.274-279
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• 2016

Objectives: We investigated bacterial diversity in the Han River water resource protection area in order to provide basic microbiological information on the drinking water safety of the Seoul metropolitan region. Methods: Samples were collected in the spring and winter, but not during the rainy season. Pyrosequencing, gene amplification, and extraction of nucleic acids were employed in this study. Results: In total, 57 and 48 operational taxonomic units were respectively analyzed in samples collected during spring and winter. Proteobacteria were predominant in all samples. The samples contained phylogenetically diverse bacterial communities, with eleven major phyla and 36 genera. Cyanobacteria were predominant in the spring samples, but not in the winter samples. The predominant species in the samples collected during both seasons belonged to the genus Aquamicrobium and Bradyrhizobium. Moreover, no pathogenic bacteria were detected in the samples. Conclusion: Proteobacteria were predominant in the samples from the Han River water source protection area. Cyanobacteria were more predominant in the spring samples than in the winter samples, but Aquamicrobium and Bradyrhizobium were predominant in both sampling seasons.

• 한국과학교육학회지
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• 제33권3호
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• pp.626-638
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• 2013

The purpose of this study was to design a development frame for multicultural science program and develop a corresponding program using the frame. This study organized a development frame to design a multicultural science education program based on the culturally responsive teaching theory. This development frame consisted of the elements of purpose, teaching examples, teaching and learning methods, and assessment. This program consisted of the guideline for class teaching courses, reading materials, student worksheet, and teachers' reference materials. Based on this development frame, a multicultural science education program was developed, which was comprised of seven activities for elementary and middle school students. Each activity of the program included the concept and inquiry related to science education. In particular, by connecting the concept of science with the cultural characteristics of various countries, the activity made students aware of cultural diversity and the commonness of science principles. Compared to other subjects, the science education field has not actively undertaken studies on multicultural education. In term of dealing with universal principles, science education is suitable for making students aware of commonness. Accordingly, this gives rise to the need to draw cultural characteristics based on basic studies such as the analysis of science textbooks in various countries and develop culturally responsive programs utilizing the outcomes.

• 한국의류산업학회지
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• 제15권1호
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• pp.1-11
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• 2013

This study analyzed the expressive characteristics of padding in contemporary fashion. The methodology of this study was quantified through documentaries, fashion collections, and internet news. The results of expressive characteristics and methods were as follow. First, variability showed the creation of dwelling space by air filling, detail mode by zipper, and diversity of recycled filling by zipper pocket. It reflected the pursuit of nomadism and subversion of permanence by change of filling. Second, it was sexual symbolicity that showed the blurred androgynous silhouette by air filling and padding bar design of the chest, stomach, shoulder for the male body silhouette. It emphasized male sexual identity and power through the expression of a modern ideal body. Third, decoration displayed various crafts (such as knitting), various fabric such as polapolis, suedette, corduroy, denim, leather, knit, and spangle. It reflected visual pleasure, scarcity and various interpretations. Fourth, playfulness character forms throug hair filling, unfamiliar fashion items, diversity of silhouette and change of uses. It reflected a pleasant feeling through astonishment and surprise, nostalgia involved in memory and childhood play and disembarrassment from reality. Fifth, simplicity showed the elimination of a sportswear's factor with regular wad quilting, elimination of wad quilting, wad quilting following the structural line of clothes and the structual line of clothes omitted. It reflected disembarrassment from stereotype and an emphasis on essential elements.

• 한국환경보건학회지
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• 제41권1호
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• pp.40-48
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• 2015

Objectives: This study aims to understand the concentration, diversity, and antibiotic characteristics of oxytetracycline resistant bacteria present in a surface water environment. Methods: Water sampling was performed in Cheongmi Stream in Gyeonggi-do, Korea in February and August 2014. Water samples collected from two sites were plated in triplicate on tryptic soy agar plates with 30 mg/L of oxytetracycline. Oxytetracycline resistant bacteria were selected from surface water in Cheongmi Stream and were subjected to 16S rDNA analysis for oxytetracycline resistant species determination. Identified resistant strains were tested for resistance to various antibiotics. Results: Results from this study indicate that the dominant resistant organisms in this aquatic environment are from family Acinetobacter and family Aeromonas. As to culturable heterotrophic bacteria, Oxytetracycline resistant bacteria were present 0.45-0.93% during winter and 0.08-0.38% during summer. Most oxytetracycline resistant bacteria exhibited resistance to more than ten of the antibiotics studied. The diversity of oxytetracycline resistant bacteria in winter was higher than in summer. Conclusion: Most of these resistant bacteria are Gram negative and are closely related to pathogenic species. These results suggest that increasing multi-antibiotic resistant bacteria in the surface water environment has a close relation to the reckless use of antibiotics in livestock.

• Environmental Engineering Research
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• 제14권1호
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• pp.3-9
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• 2009

Microorganisms play an important role in the geochemical cycles, industry, environmental cleanup, and biotechnology among other fields. Given the high microbial diversity, identification of the microorganism is essential in understanding and managing the processes. One of the most popular and powerful method for microbial identification is comparative 16S rRNA gene analysis. Due to the highly conserved nature of this essential gene, sequencing and later comparison of it against known rRNA databases can provide assignment of the bacteria into the taxonomy, and the identity of its closest relatives. Isolation and sequencing of 16S rRNA genes directly from natural environments (either from DNA or RNA) can also be used to study the structure of the whole microbial community. Nowadays, novel sequencing technologies with massive outputs are giving researchers worldwide the chance to study the microbial world with a depth that was previously too expensive to achieve. In this article we describe commonly used research approaches for the study of individual microorganisms and microbial communities using the tools provided by Ribosomal Database Project website.

• Journal of Nutrition and Health
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• 제36권5호
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• pp.500-507
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• 2003

This study was carried out to evaluate the effects on elementary school children of Internet-based nutrition education programs. The subjects consisted of 80 boys and girls (book-loaming group : 42, Internet-based teaming group : 38) in the 5th grade of elementary school. The results were as follows : The evaluation of Internet-based education programs by Internet-teaming group showed above average in all domains. As the favorite and wholesome menu chose‘nutrition kingdom’(52.6%) made of animation. The educational effect of this program was evaluated by comparing the methods of the Internet-learning group with those of the book-learning group. For effect-evaluation were measured the degree of nutrition knowledge and diversity score of dietary intake from before and after education. Before undertaking the program, there was no significant difference between the two groups in terms of their nutrition knowledge. However, the nutrition knowledge of the Internet-based teaming group after the program had increased significantly (p < 0.01) compared to the book-learning group. There was no significant difference between the two groups in terms of the diversity of dietary intake before the start of the program, but upon its completion, the Internet-based learning group showed a significant increase compared to the book-loaming group (p < 0.05). This suggests that Internet-based educational programs could be effective in drawing the interest and boosting the concentration levels of schoolchildren. In conclusion, Internet-based nutrition education shows the potential to be an effective alternative education method. (Korean J Nutrition 36(5): 500∼507, 2003)

• 한국지능시스템학회논문지
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• 제22권4호
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• pp.487-493
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• 2012

SLAM(Simultaneous Localization And Mapping)은 주변 환경에 대한 지도 작성과 자신의 위치를 인식하는 기법으로 주행 로봇 분야에서 널리 사용되고 있다. FastSLAM(A Factored Solution to the SLAM)은 파티클 필터와 확장형 칼만 필터를 기반으로 한 대표적인 SLAM 기법중의 하나이나, 재추출 단계에서 입자들의 다양성이 상실되는 문제가 제기되고 있다. 본 논문에서는 적합도 공유기법을 사용하여 입자들의 다양성 상실에 관한 문제를 보완하는 방법을 제시하고, 기존의 기법들과 성능을 비교 및 분석한다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권11호
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• pp.1515-1528
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• 2017

The gastrointestinal (GI) tract, including the rumen and the other intestinal segments of cattle, harbors a diverse, complex, and dynamic microbiome that drives feed digestion and fermentation in cattle, determining feed efficiency and output of pollutants. This microbiome also plays an important role in affecting host health. Research has been conducted for more than a century to understand the microbiome and its relationship to feed efficiency and host health. The traditional cultivation-based research elucidated some of the major metabolism, but studies using molecular biology techniques conducted from late 1980's to the late early 2000's greatly expanded our view of the diversity of the rumen and intestinal microbiome of cattle. Recently, metagenomics has been the primary technology to characterize the GI microbiome and its relationship with host nutrition and health. This review addresses the main methods/techniques in current use, the knowledge gained, and some of the challenges that remain. Most of the primers used in quantitative real-time polymerase chain reaction quantification and diversity analysis using metagenomics of ruminal bacteria, archaea, fungi, and protozoa were also compiled.

• 한국음향학회지
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• 제35권1호
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• pp.1-7
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• 2016

본 논문은 서해의 수중음향 통신 네트워크의 물리계층에 적합한 수중음향통신 시스템을 제안한다. 서해정점의 해양 관측정보와 Bellhop 음선 추적프로그램을 이용하여 서해정점의 수중 채널 모델을 구하고 이 수중 채널 모델을 이용하여 통신 변수들을 산출하고 다이버시티 기법이 응용된 CDMA(Code Division Multiple Access)와 SC-FDM(Single Carrier-Frequency Division Multiplexing) 전송방식을 이용한 음향 통신시스템을 설계한다. 설계된 통신시스템의 성능을 검증하기 위해 여러 통신환경에 대한 비트 오류율과 데이터 전송률을 연산하고 이로부터 서해안에서 긴 지연시간을 가지는 수중 채널과 낮은 Eb/No에서 사용가능한 수중 음향통신 기법을 제안한다.

• 기술혁신연구
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• 제10권1호
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• pp.22-37
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• 2002

The importance of selecting the strategic research field is increasingly emphasized by research manager and technology policy-maker in perspective of the strategic allocation of R&D resources, employing the R&D personnel, and formulating technology policy. Among various methods for selecting the strategic research field, it is, however, very difficult to find the method that involves the path-dependant trend, and interdisciplinary nature of technology development. This study suggests modified technology cluster analysis (TCA) as a method for selecting strategic research field in order to include the recent technology trend in quantitative approach. TCA is the method that groups the near technologies of which the innovation pattern is similar. TCA model can be a very necessary method for multidisciplinary government research institutes (GRIs) which conduct multi research field with forward oriented positioning. It is more difficult for them to select the strategic research field mainly due to their diversity and intangibility of research scope. In this study, we applied this method to Korea Institute of Science and Technology (KIST) which represents the most adequate research institute in terms of research diversity and forwardness. As a result of the application, we found that seven main technology groups come from TCA analysis, coincide with the technology topics of KIST's recent R&D planning. Even though this method designed for multidisciplinary research institutes, but it also can be used for establishing the research strategy of other (private or public) Research Institute which have the similar mission and scope of research.