• Asian-Australasian Journal of Animal Sciences
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• 제33권6호
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• pp.902-912
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• 2020

Objective: Mongolia is one of a few countries that supports over 25 million goats, but genetic diversity, demographic history, and the origin of goat populations in Mongolia have not been well studied. This study was conducted to assess the genetic diversity, phylogenetic status and population structure of Mongolian native goats, as well as to discuss their origin together with other foreign breeds from different countries using hypervariable region 1 (HV1) in mtDNA. Methods: In this study, we examined the genetic diversity and phylogenetic status of Mongolian native goat populations using a 452 base-pair long fragment of HVI of mitochondrial DNA from 174 individuals representing 12 populations. In addition, 329 previously published reference sequences from different regions were included in our phylogenetic analyses. Results: Investigated native Mongolian goats displayed relatively high genetic diversities. After sequencing, we found a total of 109 polymorphic sites that defined 137 haplotypes among investigated populations. Of these, haplotype and nucleotide diversities of Mongolian goats were calculated as 0.997±0.001 and 0.0283±0.002, respectively. These haplotypes clearly clustered into four haplogroups (A, B, C, and D), with the predominance of haplogroup A (90.8%). Estimates of pairwise differences (Fst) and the analysis of molecular variance values among goat populations in Mongolia showed low genetic differentiation and weak geographical structure. In addition, Kazakh, Chinese (from Huanghuai and Leizhou), and Arabian (Turkish and Baladi breeds) goats had smaller genetic differentiation compared to Mongolian goats. Conclusion: In summary, we report novel information regarding genetic diversity, population structure, and origin of Mongolian goats. The findings obtained from this study reveal that abundant haplogroups (A to D) occur in goat populations in Mongolia, with high levels of haplotype and nucleotide diversity.

• 지능정보연구
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• 제16권1호
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• pp.93-116
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• 2010

불법현금융통 적발모형 개발에 앙상블 접근방법을 사용하였다. 불법현금융통은 국내 신용카드사의 손익에 영향을 미치며 최근 국제화되고 있음에도 불구하고 학문적인 접근이 이루어지지 않았다. 부정행위 적발모형(Fraud Detection Model, FDM)은 데이터 불균형 문제로 인하여 좋은 성능을 얻기 어려운데, 다수의 모형을 결합하는 앙상블이 대안으로 제시되어 왔다. 앙상블에 포함된 모형들의 다양성이 보장된다면 단일모형에 비해 더 좋은 성능을 보인다는 점은 이미 인정되고 있으며, 최근 연구 결과는 학습된 모든 기본모형들을 사용하는 것보다 적절한 기본모형들만 선택하여 앙상블에 포함시키는 것이 바람직하다는 것이다. 본 논문에서는 효과적인 불법현금융통 적발을 위하여 축소된 앙상블 기법을 사용하는데, 정확성과 다양성 척도를 사용하여 앙상블에 참여할 기본모형을 선택하는 것이다. 다양성은 앙상블을 구성하는 기본모형들 사이의 불일치 (Disagreement or Ambiguity)를 의미하는데, FDM에 내재된 데이터 불균형문제를 고려하여 두 가지 측면에 중점을 두었다. 첫째, 학습 자료의 추출 과정에서 다양성을 확보하기 위한 소수 범주의 과잉추출 방법과 적절한 훈련 방법에 대해 설명하였다. 둘째, 소수범주에 초점을 맞추어 기존의 다양성 척도를 효과적인 척도로 변형시키고, 전진추가법과 후진소거법의 동적 다양성 계산법을 도입하여 앙상블에 참여할 기본모형을 평가하였다. 실험에 사용된 학습 알고리즘은 신경망, 의사결정수와 로짓 회귀분석이었으며, 동질적 앙상블과 이질적 앙상블을 구성하여 성능평가를 하였다. 실험결과 불법현금융통 적발모형에 있어 축소된 앙상블은 모든 기본모형이 포함된 앙상블과 성능 차이가 없었다. 축소된 앙상블은 앙상블 구성의 복잡성을 감소시키고 구현을 용이하게 한다는 점에서 FDM에서도 유력한 모형 수립 접근방법이 될 수 있음을 보였다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.31-37
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• 2019

Objective: At least eight local duck breeds have been recognized and documented as national germplasm of Indonesia so far. It is necessary to genetically characterize the local duck breeds for aiding conservation and future improvement strategies. Thus, this study was carried out to assess genetic diversity and phylogenetic relationship of eight local duck populations of Indonesia using microsatellite markers. Methods: In total, 240 individuals (30 individuals each population) from Alabio (AL), Bayang (BY), Magelang (MG), Mojosari (MJ), Pegagan (PG), Pitalah (PT), Rambon (RM), and Turi (TR) duck populations were genotyped using 22 microsatellite markers. Results: The results showed a moderate level of genetic diversity among populations, with a total of 153 alleles detected over all loci and populations, ranging from 3 to 22 alleles per locus. Observed (Ho) and expected heterozygosity (He), as well as polymorphism information content over all loci and populations were 0.440, 0.566, and 0.513, respectively. Heterozygote deficiency in the overall populations ($F_{IT}=0.237$), was partly due to the heterozygote deficiency within populations ($F_{IS}=0.114$) and moderate level of genetic differentiation among populations ($F_{ST}=0.137$). The most diverse population was MG (He = 0.545) and the least diverse population was AL (He = 0.368). The majority of populations were relatively in heterozygote deficiency (except AL), due to inbreeding. The genetic distances, phylogenetic trees, and principal coordinates analysis concluded that the populations can be grouped into two major clusters, resulting AL, MG, and MJ in one cluster separated from the remaining populations. Conclusion: The present study revealed a considerable genetic diversity of studied populations and thus, proper management strategies should be applied to preserve genetic diversity and prevent loss of alleles.

• Animal Bioscience
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• 제34권4호
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• pp.525-532
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• 2021

Objective: Old World camels are a valuable genetic resource for many countries around the world due to their adaptation to the desert environment. At present, Old World camels have encountered the challenge of unprecedented loss of genetic resources. Through our research, we would reveal the population structure and genetic variation in Old World camel populations, which provides a theoretical basis for understanding the germplasm resources and origin and evolution of different Old World camel populations. Methods: In the present study, we assessed mtDNA control region sequences of 182 individuals from Old World camels to unravel genetic diversity, phylogeography, and demographic dynamics. Results: Thirty-two haplotypes confirmed by 54 polymorphic sites were identified in the 156 sequences, which included 129 domestic and 27 wild Bactrian camels. Meanwhile, 14 haplotypes were defined by 47 polymorphic sites from 26 sequences in the dromedaries. The wild Bactrian camel population showed the lowest haplotype and nucleotide diversity, while the dromedaries investigated had the highest. The phylogenetic analysis suggests that there are several shared haplotypes in different Bactrian camel populations, and that there has been genetic introgression between domestic Bactrian camels and dromedaries. In addition, positive values of Tajima's D and Fu's Fs test demonstrated a decrease in population size and/or balancing selection in the wild Bactrian camel population. In contrast, the negative values of Tajima's D and Fu's Fs test in East Asian Bactrian camel populations explained the demographic expansion and/or positive selection. Conclusion: In summary, we report novel information regarding the genetic diversity, population structure and demographic dynamics of Old World camels. The findings obtained from the present study reveal that abundant genetic diversity occurs in domestic Bactrian camel populations and dromedaries, while there are low levels of haplotype and nucleotide diversity in the wild Bactrian camel population.

• 한국학교수학회논문집
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• 제25권1호
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• pp.61-77
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• 2022

본 연구는 고등학교 기하 교과서에 수록된 이차곡선 성질 관련 세부 학습 내용을 중심으로 교과서별 편차에 대하여 살펴보았다. 고등학교 기하 교과서에서 다루어지고 있는 내용 요소의 다양성에 대하여 비판하고 이에 대한 대안을 제시하고자 하는 것보다 내용 요소의 다양성의 실태분석에 초점을 두었다. 교육과정에서는 이차곡선의 실생활 활용적 측면을 강조하여 그 유용성과 가치를 인식하게 하도록 할 것을 명시하고 있다. 그러나 분석 결과 교육과정의 취지와 교과서의 구성이 다소 부합하지 못하고 있었으며, 교과서별 내용 요소의 편차가 상당히 큼을 확인할 수 있었다. 교수·학습의 다양성을 인정하는 측면에서 이차곡선의 도입 방식과 성질의 교과서별 다양성은 충분히 인정될 수 있는 부분이다. 그러나 대학수학능력시험과 같은 전국단위 평가를 통한 대학입시 체제를 지향하고 있는 우리의 교육 현실에서 그 결과는 사회 전반적으로 매우 민감하기 때문에 수학 교과서의 내용적 다양성은 평가적 측면에서 유불리로 해석되기도 한다. 교과서 교수 학습·내용 요소의 다양성을 인정하는 동시에 평가의 평등성 측면을 반영할 수 있는 교과서 구성에 대하여 재고가 필요한 시점이다.

• Journal of Ecology and Environment
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• 제48권1호
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• pp.32-48
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• 2024

Background: Longitudinal connectivity in river systems strongly affects biological components related to ecosystem functioning, thereby playing an important role in shaping local biodiversity and ecosystem health. Environmental DNA (eDNA)-based metabarcoding has an advantage of enabling to sensitively diagnose the presence/absence of species, becoming an efficient/effective approach for studying the community structure of ecosystems. However, little attention has been paid to eDNA-based biomonitoring for river systems, particularly for assessing the river longitudinal connectivity. In this study, by using eDNA we analyzed and compared species diversity and composition among artificial barriers to assess the longitudinal connectivity of the fish community along down-, mid- and upstream in the Hotancheon from the Geum River basin. Moreover, we investigated temporal variation in eDNA fish community structure and species diversity according to season. Results: The results of species detected between eDNA and conventional surveys revealed higher sensitivity for eDNA and 61% of species (23/38) detected in both methods. The results showed that eDNA-based fish community structure differs from down-, mid- and upstream, and species diversity decreased from down to upstream regardless of season. We found that there was generally higher species diversity at the study sites in spring (a total number of species across the sites [n] = 29) than in autumn (n = 27). Nonmetric multidimensional scaling and heatmap analyses further suggest that there was a tendency for community clusters to form in the down-, mid- and upstream, and seasonal variation in the community structure also existed for the sites. Dominant species in the Hotancheon was Rhynchocypris oxycephalus (26.07%) regardless of season, and subdominant species was Nipponocypris koreanus (16.50%) in spring and Odontobutis platycephala (15.73%) in autumn. Artificial barriers appeared to negatively affect the connectivity of some fish species of high mobility. Conclusions: This study attempts to establish a biological monitoring system by highlighting the versatility and power of eDNA metabarcoding in monitoring native fish community and further evaluating the longitudinal connectivity of river ecosystems. The results of this study suggest that eDNA can be applied to identify fish community structure and species diversity in river systems, although some shortcomings remain still need to be resolved.

• 농업과학연구
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• 제49권4호
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• pp.719-736
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• 2022

Environmental risk assessment of living modified (LM) crops is essential for their cultivation. In this study, we cultivated β-carotene enhanced transgenic soybean (LM soybean) and non-LM soybean (Gwangan) in living modified organism (LMO) isolated fields, and investigated changes in the insect fauna using three types of collection methods for three years. In total, 331,483 individual insects and arachnids, representing 82 families in 14 orders, were captured during the study. Totals of 166,518 and 164,965 individual insects and arachnids were collected from LM soybean and Gwangan, respectively. Throughout the study, although there were differences between the investigation year, region, and methods, there were no significant differences between the population densities of insect pests, natural enemies, and other insects on LM soybean and non-LM soybean. Also, there were no statistically significant differences between varieties in the results of the species diversity analysis. The data on insect species population densities were subjected to multidimensional scaling (MDS), which did not distinguish between the two varieties, LM soybean and the non-LM soybean, in all cultivated fields. However, the results of the MDS analysis were completely divided into six groups based on the yearly survey areas. These results provided the insect diversity for an environmental risk assessment of LM soybean and suggested that the guideline could be useful to detect LM crops.

• Asian-Australasian Journal of Animal Sciences
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• 제20권2호
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• pp.283-294
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• 2007

Conventional culture-based methods of enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) are being rapidly replaced by nucleic acid-based techniques which can be used to characterise complex microbial communities without incubation. The foundation of these techniques is 16S/18S rDNA sequence analysis which has provided a phylogenetically based classification scheme for enumeration and identification of microbial community members. While these analyses are very informative for determining the composition of the microbial community and monitoring changes in population size, they can only infer function based on these observations. The next step in functional analysis of the ecosystem is to measure how specific and, or, predominant members of the ecosystem are operating and interacting with other groups. It is also apparent that techniques which optimise the analysis of complex microbial communities rather than the detection of single organisms will need to address the issues of high throughput analysis using many primers/probes in a single sample. Nearly all the molecular ecological techniques are dependant upon the efficient extraction of high quality DNA/RNA representing the diversity of ruminal microbial communities. Recent reviews and technical manuals written on the subject of molecular microbial ecology of animals provide a broad perspective of the variety of techniques available and their potential application in the field of animal science which is beyond the scope of this treatise. This paper will focus on nucleic acid based molecular methods which have recently been developed for studying major functional groups (cellulolytic bacteria, protozoa, fungi and methanogens) of microorganisms that are important in nutritional studies, as well as, novel methods for studying microbial diversity and function from a genomics perspective.

• 한국간호교육학회지
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• 제20권3호
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• pp.424-434
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• 2014

Purpose: The aim of this study was to propose a teaching model to improve the cultural competence care of undergraduate Korean nursing students. Methods: A literature review was performed from 2000 through 2014 using internet to identify studies that presented nursing curricula and teaching-learning strategies in Korea. We analyzed retrieved Korean seven articles using Campinha-Bacote's ASKED model in combination with reflective experiences of authors observing classrooms in the U.S. Results: Six syllabuses presented information about cultural diversity nursing concepts in Korea. A traditional lecture focused on presenting contents about cultural knowledge by an instructor in the classroom was identified as a commonly used teaching strategy. We found that cultural competency related to cultural desire, awareness, and encounter was lacking. Thus, we developed teaching model based on the Korean nursing education contexts and combined with Campinha-Bacote model to improve cultural competency in nursing. Conclusions: The proposed teaching model shows promise as a strategy for improving the knowledge, attitudes, and skills related to cultural competent for undergraduate nursing students in Korea. However, evidences that this model improves nursing students' clinical practices reflecting cultural competent care are still lacking. Future research focusing on determining teaching methods and contents in clinical practices will be most effective.

• 한국응용곤충학회지
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• 제46권3호
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• pp.375-383
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• 2007

곤충의 채집방법, 횟수 및 시기와 채집된 곤충의 종류 및 개체수에 대한 상관관계를 조사하기 위하여 충북대학교 내 4곳을 선정, 일 년간 봄부터 가을까지 매 2주마다 채집을 실시, 총 16회 조사를 하였다. 각 장소마다 light trap, net sweeping, pitfall trap, window trap의 4가지 채집방법을 병행 실시하여 결과를 비교분석하였다. 모두 14목 672종이 조사되었으며, 전체 종수의 75%인 501종이 채집된 유아등 채집(light trap)방법이 18% 또는 그 이하의 종수를 기록한 타 채집방법들에 비해 보다 효과적인 것으로 나타났다. 또한 각 채집시기에서 채집된 종이 2주 후의 채집에서 다시 채집되는 비율이 평균 30% 정도 밖에 되지 않았다. 결과적으로 곤충다양성 조사에 있어서 보다 의미 있는 결과를 확보하기 위해서는 다양한 채집방법과 함께 채집의 빈도(sampling window)를 높이는 것이 특히 중요한 것으로 나타났다.