• 제목/요약/키워드: diversity methods

검색결과 1,261건 처리시간 0.043초

Effect of pH on soil bacterial diversity

  • Cho, Sun-Ja;Kim, Mi-Hee;Lee, Young-Ok
    • Journal of Ecology and Environment
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    • 제40권1호
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    • pp.75-83
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    • 2016
  • Background: In order to evaluate the effect of pH, known as a critical factor for shaping the biogeographical microbial patterns in the studies by others, on the bacterial diversity, we selected two sites in a similar geographical location (site 1; north latitude 35.3, longitude 127.8, site 2; north latitude 35.2, longitude 129.2) and compared their soil bacterial diversity between them. The mountain soil at site 1 (Jiri National Park) represented naturally acidic but almost pollution free (pH 5.2) and that at site 2 was neutral but exposed to the pollutants due to the suburban location of a big city (pH 7.7). Methods: Metagenomic DNAs from soil bacteria were extracted and amplified by PCR with 27F/518R primers and pyrosequenced using Roche 454 GS FLX Titanium. Results: Bacterial phyla retrieved from the soil at site 1 were more diverse than those at site 2, and their bacterial compositions were quite different: Almost half of the phyla at site 1 were Proteobacteria (49 %), and the remaining phyla were attributed to 10 other phyla. By contrast, in the soil at site 2, four main phyla (Actinobacteria, Bacteroidetes, Proteobacteria, and Cyanobacteria) composed 94 %; the remainder was attributed to two other phyla. Furthermore, when bacterial composition was examined on the order level, only two Burkholderiales and Rhizobiales were found at both sites. So depending on pH, the bacterial community in soil at site 1 differed from that at site 2, and although the acidic soil of site 1 represented a non-optimal pH for bacterial growth, the bacterial diversity, evenness, and richness at this site were higher than those found in the neutral pH soil at site 2. Conclusions: These results and the indices regarding diversity, richness, and evenness examined in this study indicate that pH alone might not play a main role for bacterial diversity in soil.

Evaluating genetic diversity and identifying priority conservation for seven Tibetan pig populations in China based on the mtDNA D-loop

  • Ge, Qianyun;Gao, Caixia;Cai, Yuan;Jiao, Ting;Quan, Jinqiang;Guo, Yongbo;Zheng, Wangshan;Zhao, Shengguo
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권12호
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    • pp.1905-1911
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    • 2020
  • Objective: Tibetan pigs, an excellent species unique to China, face serious threats, which in turn affects the development and utilization of the outstanding advantages of plateau hypoxia adaptability and reduces their genetic diversity. Therefore, a discussion of measures to conserve this genetic resource is necessary. The method, based on genetic diversity, genetic divergence and total genetic contribution rate of population, reflects the priority conservation order and varies depending on the three different purposes of conservation. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces and downloaded 564 mtDNA D-loop sequences from three indigenous pig breeds in Qinghai, Sichuan, and Yunnan Provinces distributed near the Tibetan pigs. Results: We analyzed three different aspects: Changdu Tibetan pigs have the highest genetic diversity, and from the perspective of genetic diversity, the priority conservation is Changdu Tibetan pigs. Hezuo Tibetan pigs have the highest genetic contribution, so the priority conservation is Hezuo Tibetan pigs in the genetic contribution aspect. Rkaze Tibetan pigs were severely affected by indigenous pig breeds, so if considering from the perspective of introgression, the priority conservation is Rkaze Tibetan pigs. Conclusion: This study evaluated genetic diversity and comprehensively assessed conservation priority from three different aspects in nine Tibetan pig populations.

Diversity and Composition of Tree Species in Madhupur National Park, Tangail, Bangladesh

  • Rahman, Md. Rayhanur;Hossain, Mohammed Kamal;Hossain, Md. Akhter
    • Journal of Forest and Environmental Science
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    • 제35권3호
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    • pp.159-172
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    • 2019
  • Madhupur National Park (MNP) is one of the last remaining patches of old-growth natural Sal forest left in Bangladesh where the forest is tropical moist deciduous type. A study was revealed to assess the tree species diversity and composition in this area. For determining tree species the study was conducted through extensive random quadrat survey methods with $20m{\times}20m$ sized plots. Results of the study indicated that there were 139 tree species belonging to 100 genera and 40 families. The quadrat survey assessed the basal area, stem density, diversity indices and importance value index of the tree species having ${\geq}5cm$ D.B.H (Diameter at Brest Height). The basal area and stem density of the tree species were $20.689{\pm}1.08m^2/ha$ and $1412.93{\pm}64.27stem\;ha^{-1}$ while, diversity indices, i.e. Shannon-Wiener's diversity, Simpson's evenness, Margalef's species richness and Pielou's dominance indices indicated poor diversity in comparison to that of other PAs (Protected Areas) in South-Eastern region of Bangladesh. The structural composition based on height and D.B.H through reverse-J shaped curve indicated higher regeneration and recruitment but removal of trees of large growth classes. Sal (Shorea robusta) was the most dominant tree species that accounts 75% of the total tree individuals in the natural forest patches. However, some associates of Sal, i.e. Bhutum (Hymenodictyon orixensis), Gadila (Careya arborea), and Kusum (Schleichera oleosa) etc. were seemed to be rare in MNP.

Beyond gene expression level: How are Bayesian methods doing a great job in quantification of isoform diversity and allelic imbalance?

  • Oh, Sunghee;Kim, Chul Soo
    • Journal of the Korean Data and Information Science Society
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    • 제27권1호
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    • pp.225-243
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    • 2016
  • Thanks to recent advance of next generation sequencing techniques, RNA-seq enabled to have an unprecedented opportunity to identify transcript variants with isoform diversity and allelic imbalance (Anders et al., 2012) by different transcriptional rates. To date, it is well known that those features might be associated with the aberrant patterns of disease complexity such as tissue (Anders and Huber, 2010; Anders et al., 2012; Nariai et al., 2014) specific differential expression at isoform levels or tissue specific allelic imbalance in mal-functionality of disease processes, etc. Nevertheless, the knowledge of post-transcriptional modification and AI in transcriptomic and genomic areas has been little known in the traditional platforms due to the limitation of technology and insufficient resolution. We here stress the potential of isoform variability and allelic specific expression that are relevant to the abnormality of disease mechanisms in transcriptional genetic regulatory networks. In addition, we systematically review how robust Bayesian approaches in RNA-seq have been developed and utilized in this regard in the field.

Deconstructing Opinion Survey: A Case Study

  • Alanazi, Entesar
    • International Journal of Computer Science & Network Security
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    • 제21권4호
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    • pp.52-58
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    • 2021
  • Questionnaires and surveys are increasingly being used to collect information from participants of empirical software engineering studies. Usually, such data is analyzed using statistical methods to show an overall picture of participants' agreement or disagreement. In general, the whole survey population is considered as one group with some methods to extract varieties. Sometimes, there are different opinions in the same group, but they are not well discovered. In some cases of the analysis, the population may be divided into subgroups according to some data. The opinions of different segments of the population may be the same. Even though the existing approach can capture the general trends, there is a risk that the opinions of different sub-groups are lost. The problem becomes more complex in longitudinal studies where minority opinions might fade over time. Longitudinal survey data may include several interesting patterns that can be extracted using a clustering process. It can discover new information and give attention to different opinions. We suggest using a data mining approach to finding the diversity among the different groups in longitudinal studies. Our study shows that diversity can be revealed and tracked over time using the clustering approach, and the minorities have an opportunity to be heard.

A Parallel Genetic Algorithms with Diversity Controlled Migration and its Applicability to Multimodal Function Optimization

  • YAMAMOTO, Fujio;ARAKI, Tomoyuki
    • 한국지능시스템학회:학술대회논문집
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    • 한국퍼지및지능시스템학회 1998년도 The Third Asian Fuzzy Systems Symposium
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    • pp.629-633
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    • 1998
  • Proposed here is a parallel genetic algorithm accompanied with intermittent migration among subpopulations. It is intended to maintain diversity in the population for a long period . This method was applied to finding out the global maximum of some multimodal functions for which no other methods seem to be useful . Preferable results and their detailed analysis are also presented.

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Isolation of Microorganisms for Biotechnological Application

  • Franco, Christopher-M.M.;Mcclure, Nicholas-C.
    • Journal of Microbiology and Biotechnology
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    • 제8권2호
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    • pp.101-110
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    • 1998
  • The extent of biological diversity being revealed by molecular techniques accentuates the need to develop methods to isolate and culture the large numbers of microorganisms that remain to be studied. The discovery and characterization of novel microorganisms will provide information useful in understanding microbial ecosystems and have the potential to lead to new products for the biotechnology industry. In this review, the use of innovative techniques and exploration of unusual ecosystems, that have begun to address the challenge of isolating the "uncultured" members of the microbial population, are examined.

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레일리 페이딩 채널에서 전송 안테나 다이버시티 기법을 적용한 Adaptive Modulation and Coding의 성능 분석 (Performance of Adaptive Modulation and Coding with Transmit Diversity in Rayleigh fading Channel)

  • 김인경;김주응;강창언;홍대식
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2001년도 하계종합학술대회 논문집(1)
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    • pp.73-76
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    • 2001
  • A key requirement for packet based wireless communication systems is to provide a high data rate packet service and improved throughput. To achieve a high throughput, adaptive methods for adjustment of the modulation and coding can be used. In this paper, we propose and analyze a scheme which is a combination of an adaptive modulation and coding(AMC) and transmit diversity(TD). Two different TD schemes are analysed: STTD and STD. Proposed system provides significant improvement in the average throughput.

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Rhizospheric fungi of Panax notoginseng: diversity and antagonism to host phytopathogens

  • Miao, Cui-Ping;Mi, Qi-Li;Qiao, Xin-Guo;Zheng, You-Kun;Chen, You-Wei;Xu, Li-Hua;Guan, Hui-Lin;Zhao, Li-Xing
    • Journal of Ginseng Research
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    • 제40권2호
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    • pp.127-134
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    • 2016
  • Background: Rhizospheric fungi play an essential role in the plantesoil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China. Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng. Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus. Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.

경쟁과 다양성: 방송관련법의 목적의 관점 (Competition and Diversity: Perspective of the Objectives of Broadcasting-related Laws)

  • 홍대식
    • 법제연구
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    • 제44호
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    • pp.63-101
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    • 2013
  • 이 글에서는 먼저 경쟁과 다양성이 미디어 정책의 구체적인 수단을 규정한 방송관련법이 추구하는 주된 목적이 된다는 것을 전제로 하여 각각의 개념과 구성요소 그리고 그 제도적 구현의 모습을 살펴보고, 특정분야의 경쟁법으로서의 방송관련법에서는 경쟁 목적이 차별성 요인을 갖는 한편 다양성 가치도 특수성을 갖는다는 논의를 전개한다. 다음으로 경쟁과 다양성 가치를 조화롭게 실현하기 위해서는 방송시장 특유의 구조적 규제수단을 포함하는 특별한 경쟁규칙이 필요하다고 전제하면서, 방송관련법상 경쟁 목적 규제와 다양성 목적 규제에 대하여 개선되어야 할 방향을 다음과 같이 제시한다. 첫째, 경쟁 목적 규제의 개선 방안이다. 사전적인 상태적 규제로서의 시청점유율 규제와 사후적인 행위규제로서의 금지행위 규제는 다양성 가치를 추구하면서도 원인적 규제를 대체하는 데 중요한 역할을 할 수 있다. 이를 위하여 공정거래법의 위법성 판단방식을 유추하여 다양성 관련 요소를 금지행위의 위법성 판단기준의 고려요소로 하는 방식을 구체적으로 개발할 필요가 있다. 둘째, 다양성 목적 규제의 개선 방안이다. 이는 규제 목적의 설정, 대체가능한 규제수단의 식별, 규제 목적에 맞는 규제수단과 규제수준의 선택이라는 3가지 관점에서 검토될 수 있다. 이러한 관점에서 다양성 가치가 단순한 수사적 도구에 그치지 않고 평가 및 분석 도구로 사용될 수 있는 제도적 수단 위주로 규제 틀을 개선하는 한편, 경쟁과 같은 경제적 목적과의 조화를 고려할 때 불합리해 보이는 규제에 대하여는 이를 유지할 것인가 또는 어느 정도로 유지할 것인가를 신중하게 재검토해야 한다.