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Genetic Divergence and Phylogenetic Relationships among the Korean Fireflies, Hotaria papariensis, Luciola lateratis, and Pyrocoelia rufa(Coleoptera: Lampyridae), using Mitochondrial DNA Sequences (미토콘드리아 DNA의 염기서열을 이용한 파파리반딧불이, 애반딧불이 및 늦반딧불이 (딱정벌레목: 반딧불이과)의 유전적 분화 및 계통적 관련)

  • 김익수;이상철;배진식;진병래;김삼은;김종길;윤형주;양성렬;임수호
    • Korean journal of applied entomology
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    • v.39 no.4
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    • pp.211-226
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    • 2000
  • Genetic divergence and phylogenetic relationships among the major Korean fireflies (Hotaria papariensis, Luciola lateralis, and Pyrocoelia rufa) were studied. A portion of mitochondrial COI (403 bp) and 165 rRNA (490~504 bp) genes were sequenced, and the GenBank-registered, homologous 165 rRNA sequences of Japanese fireflies were compared (27 species of Lampyridae, one of Lycidae, and one of Rhgophthalmidae). Greatest DNA and/or amino acid sequence divergence was found when P rufa, belonging to Lampyrinae was compared with H. papariensis and L. lateralis, both belong-ing to Luciolinae, confirming the current taxonomic status of the species. In the PAUP and PHYLIP analyses with 165 rRNA data, grouping of the two geographic samples of H. papariensis with H. tsushimana validate the use of generic name, Hotaria. Nevertheless, lack of sister-group relationship of the two geographic samples of H. papariensis renders further investigation on this group . Although the Korean and Japanese L. lateralis formed a strong monophyletic group, a substantial genetic differentiation was detected between them (2.9% of 165 rRNA gene sequence divergence). Finally, the geographic samples of Korean p. rufa strongly formed a group with Japanese p. rufa, warranting the use of generic name, Pyrocoelia, but the genetic distance observed between the Cheju-Island individual and all others requires further investigation on this subject. Summarized, this study supports the current taxonomic status of the Korean fireflies in that each respectively formed a strong monophyletic group with its own species or genus.

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Structural Relationships Among Factors to Adoption of Telehealth Service (원격의료서비스 수용요인의 구조적 관계 실증연구)

  • Kim, Sung-Soo;Ryu, See-Won
    • Asia pacific journal of information systems
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    • v.21 no.3
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    • pp.71-96
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    • 2011
  • Within the traditional medical delivery system, patients residing in medically vulnerable areas, those with body movement difficulties, and nursing facility residents have had limited access to good healthcare services. However, Information and Communication Technology (ICT) provides us with a convenient and useful means of overcoming distance and time constraints. ICT is integrated with biomedical science and technology in a way that offers a new high-quality medical service. As a result, rapid technological advancement is expected to play a pivotal role bringing about innovation in a wide range of medical service areas, such as medical management, testing, diagnosis, and treatment; offering new and improved healthcare services; and effecting dramatic changes in current medical services. The increase in aging population and chronic diseases has caused an increase in medical expenses. In response to the increasing demand for efficient healthcare services, a telehealth service based on ICT is being emphasized on a global level. Telehealth services have been implemented especially in pilot projects and system development and technological research. With the service about to be implemented in earnest, it is necessary to study its overall acceptance by consumers, which is expected to contribute to the development and activation of a variety of services. In this sense, the study aims at positively examining the structural relationship among the acceptance factors for telehealth services based on the Technology Acceptance Model (TAM). Data were collected by showing audiovisual material on telehealth services to online panels and requesting them to respond to a structured questionnaire sheet, which is known as the information acceleration method. Among the 1,165 adult respondents, 608 valid samples were finally chosen, while the remaining were excluded because of incomplete answers or allotted time overrun. In order to test the reliability and validity of the assessment scale items, we carried out reliability and factor analyses, and in order to explore the causal relation among potential variables, we conducted a structural equation modeling analysis using AMOS 7.0 and SPSS 17.0. The research outcomes are as follows. First, service quality, innovativeness of medical technology, and social influence were shown to affect perceived ease of use and perceived usefulness of the telehealth service, which was statistically significant, and the two factors had a positive impact on willingness to accept the telehealth service. In addition, social influence had a direct, significant effect on intention to use, which is paralleled by the TAM used in previous research on technology acceptance. This shows that the research model proposed in the study effectively explains the acceptance of the telehealth service. Second, the research model reveals that information privacy concerns had a insignificant impact on perceived ease of use of the telehealth service. From this, it can be gathered that the concerns over information protection and security are reduced further due to advancements in information technology compared to the initial period in the information technology industry, and thus the improvement in quality of medical services appeared to ensure that information privacy concerns did not act as a prohibiting factor in the acceptance of the telehealth service. Thus, if other factors have an enormous impact on ease of use and usefulness, concerns over these results in the initial period of technology acceptance may become irrelevant. However, it is clear that users' information privacy concerns, as other studies have revealed, is a major factor affecting technology acceptance. Thus, caution must be exercised while interpreting the result, and further study is required on the issue. Numerous information technologies with outstanding performance and innovativeness often attract few consumers. A revised bill for those urgently in need of telehealth services is about to be approved in the national assembly. As telemedicine is implemented between doctors and patients, a wide range of systems that will improve the quality of healthcare services will be designed. In this sense, the study on the consumer acceptance of telehealth services is meaningful and offers strong academic evidence. Based on the implications, it can be expected to contribute to the activation of telehealth services. Further study is needed to assess the acceptance factors for telehealth services, such as motivation to remain healthy, health care involvement, knowledge on health, and control of health-related behavior, in order to develop unique services according to the categorization of customers based on health factors. In addition, further study may focus on various theoretical cognitive behavior models other than the TAM, such as the health belief model.

The Comparasion of the Dynamic Stereoacuity with Two-Rods Test and Three-Rods Test (이간계와 삼간계를 이용한 동적 입체시의 비교)

  • Shim, Hyun-Suk;Kim, Sang-Moon;Kim, Sang-Hyun;Kim, Young-Cheong
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.3
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    • pp.377-384
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    • 2015
  • Purpose: On this study, we measured and compared the dynamic stereoacuity by two-rods test (Howard-Dolman Test) and three-rods test. And we analyzed the correlation between PD and refractive error with dynamic stereoacuity. Methods: Dynamic stereoacuity of two-rods test and three-rods test at 2.5 m distance for 93 adults 93 (50 males, 43 females), mean age of $21.27{\pm}2.32$ (19~32) years old, were measured 5 times for two tests. Results: The mean of dynamic stereoacuity measured by two-rods test and three-rods test were $29.91{\pm}23.03sec$ of arc and $23.75{\pm}21.65sec$ of arc for total subjects, respectively. The mean of male and female were $36{\pm}22.38sec$ of arc and $22.28{\pm}23.79$, respectively. Three-rods test showed better dynamic stereoacuity than two-rods test, but there was no statistically significant difference (p>0.05). For the average standard deviation of PD between 60.63 mm~66.19 mm, dynamic stereoacuity fo two-rod test and three-rod test were $31.48{\pm}24.87sec$ of arc and $31.48{\pm}24.87sec$ of arc, respectively. The results showed statistically significant difference (p<0.05), but the relationship between dynamic stereoacuity and PD was not great. Comparison between two tests on the basis of refractive error, dynamic stereoacuity by three-rods test was better than by two-rods test with no significant difference between both tests (p>0.05) and there was little correlation between refractive error and two dynamic stereoacuity. Conclusions: Three-rods test showing lower stereoacuity than two-rods tests could measure the lower minimum threshold of dynamic stereoacuity. It was found that both tests can be applied to dynamic stereoacuity test as a standard test, and PD and refractive error was found that little effect upon the dynamic stereoacuity. PD and refractive error was found that little effect upon the dynamic stereoacuity.

A Knowledge-based Approach for the Estimation of Effective Sampling Station Frequencies in Benthic Ecological Assessments (지식기반적 방법을 활용한 저서생태계 평가의 유효 조사정점 개수 산정)

  • Yoo, Jae-Won;Kim, Chang-Soo;Jung, Hoe-In;Lee, Yong-Woo;Lee, Man-Woo;Lee, Chang-Gun;Jin, Sung-Ju;Maeng, Jun-Ho;Hong, Jae-Sang
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.16 no.3
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    • pp.147-154
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    • 2011
  • Decision making in Environmental Impact Assessment (EIA) and Consultation on the Coastal Area Utilization (CCAU) is footing on the survey reports, thus requires concrete and accurate information on the natural habitats. In spite of the importance of reporting the ecological quality and status of habitats, the accumulated knowledge and recent techniques in ecology such as the use of investigated cases and indicators/indices have not been utilized in evaluation processes. Even the EIA report does not contain sufficient information required in a decision making process for conservation and development. In addition, for CCAU, sampling efforts were so limited that only two or a few stations were set in most study cases. This hampers transferring key ecological information to both specialist review and decision making processes. Hence, setting the effective number of sampling stations can be said as a prior step for better assessment. We introduced a few statistical techniques to determine the number of sampling stations in macrobenthos surveys. However, the application of the techniques requires a preliminary study that cannot be performed under the current assessment frame. An analysis of the spatial configuration of sampling stations from 19 previous studies was carried out as an alternative approach, based on the assumption that those configurations reported in scientific journal contribute to successful understanding of the ecological phenomena. The distance between stations and number of sampling stations in a $4{\times}4$ km unit area were calculated, and the medians of each parameter were 2.3 km, and 3, respectively. For each study, approximated survey area (ASA, $km^2$) was obtained by using the number of sampling stations in a unit area (NSSU) and total number of sampling stations (TNSS). To predict either appropriate ASA or NSSU/TNSS, we found and suggested statistically significant functional relationship among ASA, survey purpose and NSSU. This empirical approach will contribute to increasing sampling effort in a field survey and communicating with reasonable data and information in EIA and CCAU.

Object-based Building Change Detection Using Azimuth and Elevation Angles of Sun and Platform in the Multi-sensor Images (태양과 플랫폼의 방위각 및 고도각을 이용한 이종 센서 영상에서의 객체기반 건물 변화탐지)

  • Jung, Sejung;Park, Jueon;Lee, Won Hee;Han, Youkyung
    • Korean Journal of Remote Sensing
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    • v.36 no.5_2
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    • pp.989-1006
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    • 2020
  • Building change monitoring based on building detection is one of the most important fields in terms of monitoring artificial structures using high-resolution multi-temporal images such as CAS500-1 and 2, which are scheduled to be launched. However, not only the various shapes and sizes of buildings located on the surface of the Earth, but also the shadows or trees around them make it difficult to detect the buildings accurately. Also, a large number of misdetection are caused by relief displacement according to the azimuth and elevation angles of the platform. In this study, object-based building detection was performed using the azimuth angle of the Sun and the corresponding main direction of shadows to improve the results of building change detection. After that, the platform's azimuth and elevation angles were used to detect changed buildings. The object-based segmentation was performed on a high-resolution imagery, and then shadow objects were classified through the shadow intensity, and feature information such as rectangular fit, Gray-Level Co-occurrence Matrix (GLCM) homogeneity and area of each object were calculated for building candidate detection. Then, the final buildings were detected using the direction and distance relationship between the center of building candidate object and its shadow according to the azimuth angle of the Sun. A total of three methods were proposed for the building change detection between building objects detected in each image: simple overlay between objects, comparison of the object sizes according to the elevation angle of the platform, and consideration of direction between objects according to the azimuth angle of the platform. In this study, residential area was selected as study area using high-resolution imagery acquired from KOMPSAT-3 and Unmanned Aerial Vehicle (UAV). Experimental results have shown that F1-scores of building detection results detected using feature information were 0.488 and 0.696 respectively in KOMPSAT-3 image and UAV image, whereas F1-scores of building detection results considering shadows were 0.876 and 0.867, respectively, indicating that the accuracy of building detection method considering shadows is higher. Also among the three proposed building change detection methods, the F1-score of the consideration of direction between objects according to the azimuth angles was the highest at 0.891.

Estimating genetic diversity and population structure of 22 chicken breeds in Asia using microsatellite markers

  • Roh, Hee-Jong;Kim, Seung-Chang;Cho, Chang-Yeon;Lee, Jinwook;Jeon, Dayeon;Kim, Dong-kyo;Kim, Kwan-Woo;Afrin, Fahmida;Ko, Yeoung-Gyu;Lee, Jun-Heon;Batsaikhan, Solongo;Susanti, Triana;Hegay, Sergey;Kongvongxay, Siton;Gorkhali, Neena Amatya;Thi, Lan Anh Nguyen;Thao, Trinh Thi Thu;Manikku, Lakmalie
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.12
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    • pp.1896-1904
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    • 2020
  • Objective: Estimating the genetic diversity and structures, both within and among chicken breeds, is critical for the identification and conservation of valuable genetic resources. In chickens, microsatellite (MS) marker polymorphisms have previously been widely used to evaluate these distinctions. Our objective was to analyze the genetic diversity and relationships among 22 chicken breeds in Asia based on allelic frequencies. Methods: We used 469 genomic DNA samples from 22 chicken breeds from eight Asian countries (South Korea, KNG, KNB, KNR, KNW, KNY, KNO; Laos, LYO, LCH, LBB, LOU; Indonesia, INK, INS, ING; Vietnam, VTN, VNH; Mongolia, MGN; Kyrgyzstan, KGPS; Nepal, NPS; Sri Lanka, SBC) and three imported breeds (RIR, Rhode Island Red; WLG, White Leghorn; CON, Cornish). Their genetic diversity and phylogenetic relationships were analyzed using 20 MS markers. Results: In total, 193 alleles were observed across all 20 MS markers, and the number of alleles ranged from 3 (MCW0103) to 20 (LEI0192) with a mean of 9.7 overall. The NPS breed had the highest expected heterozygosity (Hexp, 0.718±0.027) and polymorphism information content (PIC, 0.663±0.030). Additionally, the observed heterozygosity (Hobs) was highest in LCH (0.690±0.039), whereas WLG showed the lowest Hexp (0.372±0.055), Hobs (0.384±0.019), and PIC (0.325±0.049). Nei's DA genetic distance was the closest between VTN and VNH (0.086), and farthest between KNG and MGN (0.503). Principal coordinate analysis showed similar results to the phylogenetic analysis, and three axes explained 56.2% of the variance (axis 1, 19.17%; 2, 18.92%; 3, 18.11%). STRUCTURE analysis revealed that the 22 chicken breeds should be divided into 20 clusters, based on the highest ΔK value (46.92). Conclusion: This study provides a basis for future genetic variation studies and the development of conservation strategies for 22 chicken breeds in Asia.

Airway size in malocclusions with hyperdivergent skeletal pattern (개방교합성 골격헝태를 갖는 부정교합자의 기도크기)

  • Kwak, So-Yeong;Kim, Hyo-Young;Jeon, Young-MI;Kim, Jong-Ghee
    • The korean journal of orthodontics
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    • v.33 no.4 s.99
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    • pp.293-305
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    • 2003
  • The pharynx have significant locations and functions because it forms a part of the unit in which respiration and deglutition are carried out. Because of the closed relationship between the pharynx and the dentofacial and craniofacial structure, many studies are carried out on this subjects. The purpose of this study were to compare the airway size including pharynx, soft palate, tongue and hyoid bone between normal and hyperdivergent skeletal pattern and to evaluate the change of those size in different age. The sample of this study were consisted of 51 subjects in normal group, 52 subjects in hyperdivergent group. Each was divided into two subgroups by age , child group(9-l2yr old) and adult group(18yr old over). The lateral cephalometric radiographs were taken and the distance, angle and ratio of the facial skeleton, pharynx, soft palate, tongue and hyoid bone were measured and evaluated statistically. The result obtained were summarized as follows : 1 SN-Mn angle, FMA, and Pal-Mn angle were significantly larger in child group than adult group, but the lower anterior facial height(LAFH) and facial height ratio(FHR) were significantly smaller in child group than adult group. Occlu-Mn angle was significantly steep in hyperdivergent group than normal, but not significantly different between child group and adult group. 2. Hyperdivergent group and child group had the stronger correlation between SN-Mn angle, FMA, Pal-Mn angle, LAFH and FHR and airway size than normal group and adult group. Hyperdivevgent child group had significant negative correlation between SN-Mn angle, FMA, Pal-Mn angle and sagittal skeletal dimension of nasopharynx and sagittal depth of nasopharyngeal lumen. Significant positive correlation were seen between LAFH and vertical measurement of airway. 3. There was no difference of the linear measurement of airway size between hyperdivergent group and normal group. 4. Adult group had smaller posterior pharyngeal wall, target nasopharyngeal depth longer nasopharyngeal height and longer pharyngeal length than child group. 5. The sagittal measurement of hyoid bone had no difference between child group and adult group. But adult group had larger vertical measurement of hyoid bone than child group.

Potential of River Bottom and Bank Erosion for River Restoration after Dam Slit in the Mountain Stream

  • Kang, Ji-Hyun;So, Kazama
    • Proceedings of the Korea Water Resources Association Conference
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    • 2011.05a
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    • pp.46-46
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    • 2011
  • Severe sediment erosion during floods occur disaster and economic losses, but general sediment erosion is basic mechanism to move sediment from upstream to downstream river. In addition, it is important process to change river form. Check dam, which is constructed in mountain stream, play a vital role such as control of sudden debris flow, but it has negative aspects to river ecosystem. Now a day, check dam of open type is an alternative plan to recover river biological diversity and ecosystem through sediment transport while maintaining the function of disaster control. The purpose of this paper is to verify sediment erosion progress of river bottom and bank as first step for river restoration after dam slit by cross-sectional shear stress and critical shear stress. Study area is upstream reach of slit check dam in mountain stream, named Wasada, in Japan. The check dam was slit with two passages in August, 2010. The transects were surveyed for four upstream cross-sections, 7.4 m, 34 m, 86 m, and 150 m distance from dam in October 2010. Sediment size was surveyed at river bottom and bank. Sediment of cobble size was found at the wetted bottom, and small size particles of sand to medium gravel composed river bank. Discharge was $2.5\;m^3/s$ and bottom slope was 0.027 m/m. Excess shear stress (${\tau}_{ex}$) was calculated for hydraulic erosion by subtracting the values of critical shear stress (${\tau}_{c}$) from the value of shear stress (${\tau}$) at river bottom and bank (${\tau}_{ex}=\tau-{\tau}_c$). Shear stress of river bottom (${\tau}_{bottom}$) was calculated using the cross-sectional shear stress, and bank shear stress (${\tau}_{bank}$) was calculated from the method of Flintham and Carling (1988). $${\tau}_{bank}={\tau}^*SF_{bank}((B+P_{bed})/(2^*P_{bank}))$$ where $SF_{bank}=1.77(P_{bed}/p_{bank}+1.5)^{-1.4}$, B is the water surface width, $P_{bed}$ and $P_{bank}$ are wetted parameter of the bed and bank. Estimated values for ${\tau}_{bottom}$ for a flow of $2.5\;m^3/s$ were lower as 25.0 (7.5 m cross-section), 25.7 (34 m), 21.3 (86 m) and 19.8 (150 m), in N/$m^2$, than critical shear stress (${\tau}_c=62.1\;N/m^2$) with cobble of 64 mm. The values were insufficient to erode cobble sediment. In contrast, even if the values of ${\tau}_{bank}$ were lower than the values for ${\tau}_{bottom}$ as 18.7 (7.5 m), 19.3 (34 m), 16.1 (86 m) and 14.7 (150 m), in N/$m^2$, excess shear stresses were calculated at the three cross-sections of 7.5 m, 34 m, and 86 m distances compare with ${\tau}_c$ is 15.5 N/$m^2$ of 16mm gravel. Bank shear stresses were sufficient for erosion of the medium gravel to sand. Therefore there is potential to erode lateral bank than downward erosion in a flow of $2.5\;m^3/s$. Undercutting of the wetted bank can causes bank scour or collapse, therefore this channel has potential to become wider at the same time. This research is about a potential of sediment erosion, and the result could not verify with real data. Therefore it need next step for verification. In addition an erosion mechanism for river restoration is not simple because discharge distribution is variable by snow-melting or rainy season, and a function for disaster control will recover by big precipitation event. Therefore it needs to consider the relationship between continuous discharge change and sediment erosion.

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A Correlation Between Vertical Fixation Disparity and Stereopsis at Near (근거리에서 수직주시시차와 입체시와의 관계)

  • Yoon, Min-Hwa
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.3
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    • pp.321-327
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    • 2013
  • Purpose: To investigate a correlation between vertical fixation disparity and stereopsis by analyzing distribution of types of vertical fixation disparity. Methods: In this study, RANDOT(R) stereotest partly using random dot was used in stereopsis tests. The vertical fixation disparity in close proximity categorized fixation disparity slope into six types on the basis of the result of measurement in when two thin lines, one for one eye looks, become overlapped as a line by adding into prism with Wesson fixation disparity card while maintaining a distance of 25 cm from patients. Results: In the types of near vertical fixation disparity curve, targeting 43 people, the first type 55.82% was the most distributed, the second type is the least 23.25%, the third type of 4.65%, the fourth type of 4.65%, the fifth type of 6.98%, the sixths types of 4.65% were distributed. The result of a correlation analysis, which shows the degree of linear correlation between two variables, represented that stereopsis is not correlated with Y-intercept (r = -0.07) which show vertical fixation disparity, associated phoria (r = -0.03) and dissociated phoria (r = -0.00), but it was not statistically significant (p>0.05). Fixation disparity slope of vertical fixation disparity (r = 0.36) was shown to have a positive correlation with stereopsis. It has a low positive correlation and a meaningful statistics (p<0.05). The Y-intercept which indicates vertical fixation disparity was also not associated with stereopsis (r = -0.07) and dissociated phoria (r = -0.03), and this was not statistically significant (p>0.05), while it had a high correlation as well as a statistically significant with associated phoria (r = 0.89). There was a negative correlation between Y-intercept and fixation disparity slope of vertical fixation disparity (r = -0.33). It showed a low relationship but statistically valuable (p>0.05). As a result of regression analysis, the stereopsis was changed as 7.631" if vertical fixation disparity changes 1' and the vertical fixation disparity changed as 0.017' if stereopsis changes 1', and the change was statistically significant (p<0.05). Conclusions: The study shows that there is a low correlation between stereopsis and vertical fixation disparity, and it is difficult to determine stereopsis only using vertical fixation disparity. Therefore, it suggests other factors above vertical fixation disparity have greater influence on stereopsis.

Effect of X-Irradiation on the Levels of some Sulfhydryl Groups, Protein and Cell Volume of Ehrlich Ascites Tumour Cells (X-선(線) 조사(照射)가 Ehrlich 암세포(癌細胞)의 용적(容積), 단백양(蛋白量) 및 수종(數種) Sulfhydryl 기(基)에 미치는 영향(影響)에 관(關)하여)

  • Yu, Choon-Shik;Choo, Young-Eun
    • The Korean Journal of Physiology
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    • v.3 no.2
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    • pp.9-16
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    • 1969
  • It is well known that a number of -SH and -SS containing substances afford a certain measure of protection against radiation effects in many biological systems, and it is conceivable that inherent -SH levels in Ehrlich ascites tumour (ELD)cells may be of decisive improtance with respect to the development of cellular radiation injury. So far, little effort has been directed to elucidate the changes in levels of different -SH and -SS groups in ELD cells when the tumour-bearing whole animal was subjected to the sublethal dose of X-irradiation. The present study was designed to bring some lights in the possible changes of and relationship between various sulfhydryl levels, such as P-SH, NP-SH and NP-SS, as well as the content of protein and cell volume of ELD cells, after subjecting the ELD mice to 1,200 r of X-irradiation. The animals used in this experiment were all mixed bred mice of $20{\sim}25\;gm$ in body weight (approximately 2 months old) irrespective of sex. 12 mice in one experiment were inoculated intraperitoneally with 0.2 ml of ascites tumour cells $(2{\times}10^6\;cells)$, and on the 7th day of the tumour growth, they were X-irradiated with 1,200 r, using the conventional X-ray machine under the following conditions: 200 Kv at 15 mA, 0.5 mm Cu filter, target-skin distance: 50 cm. Radiation dose was measured with the the Philip integrating dosimeter. At 24, 36, 48 and 60 hours after the X-irradiation, the mice were killed by cervical dislocation, and the tumours were taken out. Freshly withdrawn ascites tumours were placed in ice, and immediately the cell concentration was measured with the Coulter Cell Counter (Model B), and the hematocrit of the tumour cells were also determined. Cell volume was thus calculated by the cell concentration and hematocrit value. P-SH content of ELD cells was measured potentiometrically according to the method of Calcutt & Doxey, and NP-SH and NP-SS contents were measured spectrophotometrically by the method described by Ellman. Protein content of ELD cells was determined with the Folin phenol reagent by Lowry et al. Altogether, 48 experimental mice were used, and 12 mice with the only exception of X-irradiation were used as the control. Results obtained indicate that the contents of all the cellular sulfhydryl groups as well as cell volume and protein content of the ELD cells increase significantly as time progresses after the sub-lethal X-ray dose of 1,200 r was given and that all the increase is in a lineal fashion. The regression lines of the relative values, (i. e., taking each control value as 1) of all the values obtained, and the regression lines of cell volume, protein and NP-SH are identical, whereas those of NP-SS and P-SH appear to be widely seperated. However, the difference of those two lines (NP-SS & P-SH) were found to be not significant statistically (p>0.05). Therefore, it can be concluded from the above results that all the values examined increase in a lineal fashion with no statistically significant difference among them. Also, with the radiation dose of 1,200 r, the ELD cell becomes enlarged and swollen progressively up to 60 hours post-irradiation and it becomes more than two times of the original normal size at 60 hours after the irradiation, and up to this stage, it seems apparent that the cell division has been slow due to the X-irradiation applied in this experiment. It is well understandable that the contents of NP-SH, NP-SS, P-SH and protein of the ELD cells increase in parallel with the increase of the cell volume by the X-ray does used, but it also seems interesting to note that all the cellular substances tested show no appreciable difference in the pattern of increase.

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