• 제목/요약/키워드: dissolved oxygen

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용존산소 농도 조절이 미생물유래 Transglutaminase 생산에 미치는 영향 (The Effect of Dissolved Oxygen on Microbial Transglutaminase production by Streptoverticillium morbaraense)

  • 유재수;전계택;정용섭
    • KSBB Journal
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    • 제18권2호
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    • pp.155-160
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    • 2003
  • Streptoverticillium morbaraene로부터 미생물 유래 transglutaminase 생산을 위하여 최적의 용존산소 농도를 구명하였다. 용존산소는 용존산소 농도 자동 조절 시스템에 의해 조절되었다. 발효 중 용존산소 농도 조절을 위하여 통기속도는 0.3-3.9 L/min, 교반속도는 260-360 rpm으로 각각 범위를 설정하였다. 용존산소 농도를 조절한 다양한 회분식 배양에서 용존산소가 20%일 때 최대 미생물유래 transgiutaminase 생산이 가능하였다. 최분배양에서 용존산소 농도를 20%로 조절한 경우 미생물유래 transglutaminase 생산은 2.12 U/mL이었고, 용존산소를 조절하지 않은 회분식 배양의 미생물유래 transglutaminase 생산보다 1.1배 향상되었다. 역시 가장 높은 미생물유래 transglutaminase 생산은 용존산소를 20%로 조절한 유가식 배양에서 가능하였으며, 용존산소를 조절하지 않은 회분식 배양의 미생물유래 transglutaminase 생산에 비교해서 1.3배 증가하였다. 최대 건조균체량과 미생물유래 transglutaminase 생산은 각각 13.2 g/L와 2.6 U/mL이었다. 용존산소를 20%로 용존산소 농도 자동 조절 시스템에 의해 조절한 유가식 배양은 미생물유래 transgiutaminase 생산에 적절하였으며 다른 미생물 배양에도 적용할 수 있을 것으로 판단된다.

FET형 용존산소 센서 어레이 측정시스템 (A Dissolved Oxygen Measurement System Using FET-type Dissolved Oxygen Sensor Array)

  • 정훈;손병기
    • 센서학회지
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    • 제10권4호
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    • pp.259-265
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    • 2001
  • FET형 용존산소 센서는 pH-ISFET를 기본 소자로하여 pH 감지 게이트 근처에 백금으로 된 작업 전극을 형성한 구조를 가지고 있다. 작업전극에 특정한 전위를 인가하면 전기분해로 인하여 용존산소의 농도에 비례하는 수소 이온이 pH 감지 게이트 주변에 발생된다. 따라서 pH-ISFET를 통하여 수소 이온 농도의 변화량을 검출하면 용존산소의 농도를 측정할 수 있게된다. 본 논문에서는 이러한 FET형 용존산소 센서를 어레이 형태로 제작하여 적용하고 측정의 신뢰성을 높이기 위한 알고리즘을 도입한 FET형 용존산소 센서 어레이 측정 시스템을 개발하였다. 또한 상용 용존산소 측정기와 그 성능을 비교 분석하였다.

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압력 및 용존산소 농도가 활성슬러지에 미치는 영향 (Effects of Pressure and Dissolved Oxygen Concentration on the Activated Sludge)

  • 양병수;신현무
    • 한국환경과학회지
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    • 제4권3호
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    • pp.259-267
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    • 1995
  • This study was conducted to evaluate the effects of pressure and dissolved oxygen concentration on the activated slut비e and to determine the optimum depth of deep shaft process. Some results from this study were summarized as follows. 1. It is considered that low sludge product in the activated sludge system maintaining high dissolved oxygen concentration is attributed to the increase of endogeneous respiration rate caused by the increase of aerobic zone in the sludge floe. 2. The increase of dissolved oxygen concentration does not affect to the increase of organic removal efficiency greatly and therefore the limiting factor is the substrate transfer into the inner part of floe. 3. The yield coefficient, Y is decreased in proportion to the increase of oxygen concentration. In this study, Y values arre ranged from 0.70 to 0.41 according to the variation of dissolved oxygen concentration from 18.0mg/$\ell$ to 258 mg/$\ell$. 4. The optimum depth of deep shaft process should be determined within the limits of non-toxicity to the microorganism and it is about loom in this study.

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Clark Cell을 이용한 ppb단위 용존산소 측정기술개발 (Development of pub Level Dissolved Oxygen Measuring Technology using Clark Cell)

  • 정경열;류길수
    • Journal of Advanced Marine Engineering and Technology
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    • 제26권3호
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    • pp.375-381
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    • 2002
  • Measuring of the dissolved oxygen is widely used for the environmental control of natural waters, sewage waste treatment, medical and biochemical studies, soil husbandry, food and drug process control, and prevention of corrosion in boilers. Especially, a power plants need special management for preventing accidents from corrosion, therefore, it is essential to measure the concentration of dissolved oxygen in real-time. In this paper we present a method of measuring dissolved oxygen very accurately up to pub units. This method, called polarograph method, is based on the measures of the electric current generated by the oxidation process in cathode and de-oxidation process in anode, assuming that the amount of the current is proportional to the density of dissolved oxygen.

Clark Cell을 이용한 ppb Level 용존산소 측정기술 개발에 관한 연구 (A Study on the Development of ppb Level Dissolved Oxygen Measuring Technology using Clark Cell)

  • 정경열;이후락;동은석;이수태;류길수
    • 한국마린엔지니어링학회:학술대회논문집
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    • 한국마린엔지니어링학회 2001년도 춘계학술대회 논문집
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    • pp.197-201
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    • 2001
  • Measuring of the dissolved oxygen is widely used for the environmental control of natural waters, sewage waste treatment, medical and biochemical studies, soil husbandry, food and drug process control, and prevention of corrosion in boilers. Especially, a power plants need special management for preventing accidents from corrosion, therefore, it is essential to measure the concentration of dissolved oxygen in real-time. In this paper we present a method of measuring dissolved oxygen very accurately up to ppb units. This method, called polarographic method, is based on the measures of the electric current generated by the oxidation process in cathode and de-oxidation process in anode, assuming that the amount of the current is proportional to the density of dissolved oxygen.

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재포기 계수, 1차 생산율 및 호흡률을 이용한 하천의 용존산소 추정 (Estimation of Dissolved Oxygen in Streams using Reaeration, 1st Production and Respiration Rates)

  • 김경섭;황성규
    • 대한환경공학회지
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    • 제31권6호
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    • pp.428-433
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    • 2009
  • 용존산소는 하천의 중요한 수질관리 항목중의 하나로 1세기 전부터 언급되어 왔으며, 농도가 낮을 경우 물고기 폐사를 유발하기도 한다. 환경과학자 및 공학자들은 하천의 용존산소 파악을 위한 결정론적 모델을 제안하였으며, 용존산소가 공간적으로 변하지 않을 경우 적용 가능한 델타 방법(Delta Method), 근사 델타 방법(Approximate Delta Method), 극한값 방법(Extreme Value Method) 및 최적화 방법(Optimization Method)등을 소개하였다. 이 방법들은 주로 용존산소와 관련 있는 매개변수 즉, 재포기 계수, 1차 생산율 및 호흡률 등을 산정하여, 이로부터 일주기 또는 년주기 용존산소를 파악한다. 본 논문에서는 용존산소 파악을 위한 각 방법을 간단히 소개하며, 안성천 유역의 금석천 및 안성천 본류, 두 지점에 적용하여 각 방법의 한계와 장 단점을 파악하였다. 이를 기초로 비용/효과적인 용존산소 파악 방법을 제안하였다.

하수처리장 폭기조의 용존산소농도 모니터링 대표지점 선정에 관한 연구 (A study on the determination of a representative location for monitoring the dissolved oxygen concentration in a aeration tank of sewage treatment plant)

  • 방석용;범봉수;김진한
    • 상하수도학회지
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    • 제33권5호
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    • pp.389-394
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    • 2019
  • In order to determine the location of average concentration and distribution status of dissolved oxygen in the rectangular aeration tank of the sewage treatment plant was analyzed and the difference of dissolved oxygen concentration was remarkable at each location. Compared with the computational fluid dynamics analysis, it was found that the results were consistent with the measurement results by showing the difference of dissolved oxygen concentration between the locations. Based on the measured data, the representative location of dissolved oxygen in aeration tank was selected by using statistical analysis method and the representative location was expressed in three-dimensional coordinates(LWH : 25%, 50%, 33%) from flow direction and left wall. Also the difference between the dissolved oxygen concentration at the actual measurement location and the average concentration value of the entire aeration tank was founded, and the equations for calibrating the automatic measurement data considering the actual measurement location were calculated.

이젝터-폭기 시스템의 용존산소특성에 미치는 염도의 영향 (Effect of Salinity on Dissolved Oxygen Characteristics in an Ejector-Aerator)

  • 양희천;박상규
    • Journal of Advanced Marine Engineering and Technology
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    • 제35권5호
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    • pp.640-646
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    • 2011
  • 용존산소는 물속에 용해되어 있는 산소량으로 수질의 지표가 된다. 본 논문은 이젝터-폭기 시스템의 용존산소특성에 미치는 염도의 영향에 대한 실험적 연구를 목적으로 한다. 이젝터-폭기 시스템은 모터-펌프, 이젝터, 모터-블로어, 폭기 및 순환수조, 제어판넬 등으로 구성된다. 처리수의 용존산소량은 염도가 증가함에 따라 감소하였으며, 산소전달율의 정량적인 변수인 총괄 물질전달계수는 염도가 증가함에 따라 증가하였다.

Effect of Dissolved Oxygen Concentration on the Metabolism of Glucose in Pseudomonas putida BM014

  • Park, Won-Jae;Lee, Eun-Yeol;Park, Cha-Yong
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제3권2호
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    • pp.109-111
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    • 1998
  • The effect of dissolved oxygen concentration on the metabolism of glucose in Pseudomonas putida BM014 was investigated. Glucose was completely converted to 2-ketogluconate via extracellular oxidative pathway and then taken up for cell growth under the condition of sufficient dissolved oxygen concentration. On the other hand, oxygen limitation below dissolved oxygen tension (DOT) value of 20% of air saturation caused the shift of glucose metabolism from the extracellular oxidative pathway to the intracellular phosphorylative pathway. Specific activities of hexokinase and gluconate kinase in intracellular phosphorylation pathway decreased as the DOT increased, while 2-ketogluconokinase activity in extracellular oxidative pathway increased under the same condition. This result can be usefully applied to microbial transformation of glucose to 2-ketogluconate, the synthetic precursor for iso-vitamine C, with almost 100% yield via extracellular oxidation by simple DOT control.

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Effect of Dissolved Oxygen on the Stress Cor rosion Cracking Behavior of 3.5NiCrMoV Steels in High Temperature Water

  • Lee, J.H.;Maeng, W.Y.;Kim, U.C.
    • Corrosion Science and Technology
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    • 제2권4호
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    • pp.178-182
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    • 2003
  • Slow Strain Rate Tests (SSRT) were carried out to investigate the effect of environmental factors on the Stress Corrosion Cracking (SCC) susceptibility of 3.5NiCrMoV steels used in discs for Low-Pressure (LP) steam turbines in electric power generating plants. The influences of dissolved oxygen on the stress corrosion cracking of turbine steel were studied, For this purpose, specimens were strained at variously oxygenated conditions at $150^{\circ}C$ in pure water. When the specimen was strained with $1{\times}10^{-7}s^{-1}$ at $150^{\circ}C$ in pure water, increasing concentration of dissolved oxygen decreased the elongation and the UTS. The corrosion potential and the corrosion rare increased as the amounts of dissolved oxygen increased. The increase of the SCC susceptibility of the turbine steel in a highly dissolved oxygen environment is due to the non protectiveness of the oxide layer on the turbine steel surface and the increase of the corrosion current. These results clearly indicate that oxygen concentration increases Stress Corrosion Cracking susceptibility in turbine steel at $150^{\circ}C$.