• Title/Summary/Keyword: dissolved oxygen

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Development of a Portable Potentiostat with Wireless Communications for Measuring Dissolved Oxygen (용존산소 측정을 위한 무선통신 기반 휴대형 포텐쇼스탯 개발)

  • Lee, Hyun-Seok;Han, Ji-Hoon;Pak, Jungho
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.12
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    • pp.1641-1647
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    • 2018
  • In this paper, we describe a portable potentiostat which is capable of cyclic voltammetry(CV) and amperometry for electrochemical dissolved oxygen sensor. In addition, this portable potentiostat can also transmit the measured data wirelessly to android devices such as smart phone, tablet, etc. through Bluetooth. The potentiostat system consists of three parts; a voltage generator circuit which is controlled by Arduino nano and 12-bit DAC(digital to analog converter) to generate necessary electric potential for operating the electrochemical sensor, an oxidation/reduction current measurement circuit, and a Bluetooth module to transmit data wirelessly to an android device. Once measurements are carried out with the android application, the measured data is transmitted to the android device via Bluetooth and displayed using the android app. in real time. In this paper, we report the measured reduction current with a fabricated dissolved oxygen sensor in both saturated-oxygen state and zero-oxygen states. The results of the developed portable potentiostat system are in good agreement with those of the commercial portable potentiostat (${\mu}stat200$, Dropsens inc.). The measured peak reduction currents using the developed potentiostat and the commercial ${\mu}stat200$ potentiostat were $-0.755{\mu}A$ and $-0.724{\mu}A$, respectively. The reduction currents measured at zero-oxygen state were $-0.005{\mu}A$ and $-0.004{\mu}A$. The discrepancy between those two systems seems very small, which implies successful development of a portable and wireless potentionstat.

Leakage Prevention of Mechanical Seal in a Feed Water Pumpof Power Plant with Oxygen Water Treatment (산소주입 수처리 발전소용 급수펌프 밀봉장치의 누설방지)

  • Shin, Jung-Gook;Moon, Seung-Jae;Yoo, Hoseon
    • Plant Journal
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    • v.7 no.1
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    • pp.56-63
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    • 2011
  • The identifying cause of leakage problems on mechanical seal in a feed water pump of power plant have been difficult because of technical limits of domestic mechanical seal manufacturer and the various conditions of power plants. This paper can get a conclusion through the consideration study and experiment test of mechanical seal characteristics of feed water pump depending on oxygen treatment as follows. The oxygen treatment increase dissolved oxygen and cause a corrosion on the mechanical seal stationary ring composed of antimony as well as leakage problems. For solving leakage problems of seal, to eliminate dissolved oxygen in the cooling water of seal with hydrazine injection can prevent leakage problems of mechanical seal in feed water pump.

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Nitrospira Community Composition in Nitrifying Reactors Operated with Two Different Dissolved Oxygen Levels

  • Park, Hee-Deung;Noguera, Daniel R.
    • Journal of Microbiology and Biotechnology
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    • v.18 no.8
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    • pp.1470-1474
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    • 2008
  • Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and terminal restriction fragment length polymorphism analyses based on 168 rRNA gene sequences revealed that the Nitrospira community compositions of the two reactors during the early period related to group 1 and half of the Nitrospira community composition shifted to group 2 in the high-DO reactor after day 179, although there was no significant change in the low-DO reactor. These results suggested that DO was an important factor affecting Nitrospira community compositions in the nitrifying reactors.

On-line monitoring of microorganism cultivation processes using optical sensing membranes for simultaneous detection of dissolved oxygen and pH (용존산소와 pH의 동시 검출용 광학 센서 막을 이용한 미생물 발효공정의 온라인 모니터링)

  • Kim, Chun-Kwang;Rhee, Jong-II
    • KSBB Journal
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    • v.24 no.1
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    • pp.106-112
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    • 2009
  • An optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen(DO) and pH value simultaneously. It has employed HPTS as a pH sensitive dye and a ruthenium(II) complex as a DO sensitive dye. The sensing membrane has been applied to wells in a 24-well microtiter plate. Using the 24-well microtiter plate the concentrations of dissolved oxygen and pH values have been on-line monitored during the cultivations of E.coli DH5${\alpha}$, B.cereus 318 and P.pastoris X-33. On-line monitoring of DO and pH in microorganism cultivation processes showed good performance of the sensing membrane containing 5 mM HPTS and 2 or 5 mg/mL Rudpp.

Effect of Dissolved Oxygen on the Growth of Azotobacter vinelandii UWD and Production of PHBV in the Mixture of Organic Acids and Glucose (유기산 및 포도당 혼합배지에서 Azotobacter vinelandii UWD의 생장 및 PHBV 생산에 대한 용존산소의 영향)

  • 박창호
    • KSBB Journal
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    • v.13 no.6
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    • pp.675-680
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    • 1998
  • In both 7L and 20L fermentor experiments the level of dissolved oxygen (D.O) strongly affected growth and PHBV production of Azotobacter vinelandii UWD. A higher D.O. increased carbon substrate consumption rate and cell growth rate with a similar residual biomass production. However, a lower D.O. was a much better condition for PHBV production. In a 20L fermentor experiments controlled at 5% D.O. cell growth rate was about twice faster(0.555 hr-1 and 0.260 hr-1 at the acid and the glucose phase, respectively) with an equal amount(4.5 g/L) of residual biomass production. However, PHBV content in the cell(62.3 wt%) increased 17.3 times at 1% D.O.

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ANN-XGB based predictions of dissolved oxygen (ANN-XGB를 이용한 수중 산소 농도 예측)

  • Jo, Gwanghyun;Lee, Keun Young
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2022.10a
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    • pp.457-458
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    • 2022
  • The dissolved oxygen (DO) is one of the factors of ecosystem that affects survival of aquatic life. Artificial neural network - XGboost (ANN-XGB), which was trained by water quality and weather data obtained at Anyang-streamto, was employed to forecast DO after 1 hours. We document the perforamnce of ANN-XGB.

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Cultivation of Sprout by Highly Concentrated Oxygen Water Soaking (고농도 산소수 침지를 통한 새싹 재배)

  • Cha, Jin-Myeong;Hong, Seung-Ho;Kim, Sun-Yil;Park, Ju-Young;Kim, Maeng-Su;Lee, In-Hwa
    • KSBB Journal
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    • v.23 no.6
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    • pp.525-528
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    • 2008
  • In order to compare the germination and growth rate of the sprouts soaked in highly concentrated oxygen water, it with specific amounts of oxygen dissolved was produced in a high pressure reactor by pressuring oxygen. The sprouts were observed after being soaked in $20^{\circ}C$ oxygen water with 20, 30, 40, 50 ppm of oxygen dissolved each. Results of ten days later indicate that the final germination rate of the sprout soaked in 50 ppm oxygen water was $24.6{\sim}28.6%$ higher than that of the sprout soaked in distilled water. The final growth length also measured 6-7 mm higher than the sprout soaked in distilled water, demonstrating that enough supply of oxygen to the sprout induces stability and efficiency in its growth.

Application of CE-QUAL-W2 [v3.2] to Andong Reservoir: Part I: Simulations of Hydro-thermal Dynamics, Dissolved Oxygen and Density Current

  • Bhattarai, Prasid Ram;Kim, Yoon-Hee;Heo, Woo-Myoung
    • Korean Journal of Ecology and Environment
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    • v.41 no.2
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    • pp.247-263
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    • 2008
  • A two-dimensional (2D) reservoir hydrodynamics and water quality model, CE-QUAL-W2, is employed to simulate the hydrothermal behavior and density current regime in Andong Reservoir. Observed data used for model forcing and calibration includes: surface water level, water temperature, dissolved oxygen and suspended solids concentration. The model was calibrated to the year of 2003 and verified with continuous run from 2000 till 2004. Without major adjustments, the model accurately simulated surface water levels including the events of large storm. Deep-water reservoirs, like Andong Reservoir, located in the Asian Monsoon region begin to stratify in summer and overturn in fall. This mixing pattern as well as the descending thermocline, onset and duration of stratification and timing of turnover phenomenon were well reproduced by the Andong Model. The temperature field and distinct thermocline are simulated to within $2^{\circ}C$ of observed data. The model performed well in simulating not only the dissolved oxygen profiles but also the metalimnetic dissolved minima phenomenon, a common1y occurring phenomenon in deep reservoirs of temperate regions. The Root Mean Square Error (RMSE) values of model calibration for surface water elevation, temperature and dissolved oxygen were 0.0095 m, $1.82^{\circ}C$, and $1.13\;mg\;L^{-1}$, respectively. The turbid storm runoff, during the summer monsoon, formed an intermediate layer of about 15 m thickness, moved along the metalimnion until being finally discharged from the dam. This mode of transport of density current, a common characteristic of various other large reservoirs in the Asian summer monsoon region, was well tracked by the model.

Annual cycles of nutrients and dissolved oxygen in a nutrient-rich temperate coastal bay, Chinhae Bay, Korea (영양염류가 풍부한 온대 해역 내만(한국, 진해만)에서의 영양염류와 용존산소의 연변화)

  • HONG, GI HOON;KIM, KYUNG TAE;PAE, SE JIN;KIM, SUK HYUN;LEE, SOO HYUNG
    • 한국해양학회지
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    • v.26 no.3
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    • pp.204-222
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    • 1991
  • The annual cycles of plant major nutrients and dissolved oxygen in a nutrients-rich semi-enclosed coastal inlet, chinhae Bay, of the southern coast of the Korean Peninsula are first presented. The water column of the bay is stratified during summer (April-late September) and well0mixed during winter (October-March). During the summer stratification period, dissolved oxygen contents exceed 400uM in the surface but diminish to less than 50uM in the near bottom waters, which often results in an anoxic environment in the inner part of Chinhae Bay. After the breakdown of the stratification in October, dissolved oxygen concentration remains undersaturated until February. The evidence of allochthonous input of N-nutrients throughout the year is readily seen in the water column: however. crude budget calculations show that the nutrients are efficiently utilized within the bay ecosystem, and that export of the nutrients from the bay to the shelf must be negligible. There is no sign of the enrichment of the nutrients in the water column. The eutrophication phenomenon sensu stricto is not observed in chinhae Bay. Using the standing stock of dissolved oxygen and estimation of the oxygen fluxes across the air-sea boundary, a benthic oxygen respiration rate during winter is estimated conservatively at 21-24 mmol Cm/SUP -2/d/SUP -1/. this oxygen respiration rate accounts for about 20% of the total phytoplankton production in winter.

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Control of dissolved Oxygen Concentration and Specific Growth Rate in Fed-batch Fermentation (유가식 생물반응기에서의 용존산소농도 및 비성장속도의 제어)

  • Kim, Chang-Gyeom;Lee, Tae-Ho;Lee, Seung-Cheol;Chang, Yong-Keun;Chang, Ho-Nam
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.354-365
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    • 1993
  • A novel control method with automatic tuning of PID controller parameters has been developed for efficient regulation of dissolved oxygen concentration in fed-batch fermentations of Escherichia coli. Agitation speed and oxygen partial pressure in the inlet gas stream were chosen to be the manipulated variables. A heuristic reasoning allowed improved tuning decisions from the supervision of control performance indices and it coule obviate the needs for process assumptions or disturbance patterns. The control input consisted of feedback and feedforword parts. The feedback part was determined by PID control and the feedforward part is determined from the feed rate. The proportional gain was updated on-line by a set of heuristics rules based on the supervision of three performance indices. These indices were output error covariance, the average value of output error, and input covariance, which were calculated on-line using a moving window. The integral and derivative time constants were determined from the period of output response. The specific growth rate was maintained at a low level to avoid acetic acid accumulation and thus to achieve a high cell density. The specific growthe rate was estimated from the carbon dioxide evolution rate. In fed-batch fermentation, the simutaneous control of dissolved oxygen concentration (at 0.2; fraction of saturated value) and specific growth rate (at 0.25$hr^{-1}$) was satisfactory for the entire culture period in spite of the changes in the feed rate and the switching of control input.

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