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On-line monitoring of microorganism cultivation processes using optical sensing membranes for simultaneous detection of dissolved oxygen and pH  

Kim, Chun-Kwang (Department of Material and Biochemical Engineering)
Rhee, Jong-II (School of Applied Chemical Engineering)
Publication Information
KSBB Journal / v.24, no.1, 2009 , pp. 106-112 More about this Journal
Abstract
An optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen(DO) and pH value simultaneously. It has employed HPTS as a pH sensitive dye and a ruthenium(II) complex as a DO sensitive dye. The sensing membrane has been applied to wells in a 24-well microtiter plate. Using the 24-well microtiter plate the concentrations of dissolved oxygen and pH values have been on-line monitored during the cultivations of E.coli DH5${\alpha}$, B.cereus 318 and P.pastoris X-33. On-line monitoring of DO and pH in microorganism cultivation processes showed good performance of the sensing membrane containing 5 mM HPTS and 2 or 5 mg/mL Rudpp.
Keywords
Process monitoring; Optical sensing membrane; DO; pH; Microorganism;
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