• Title/Summary/Keyword: diseases and pests

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Occurrence of Sporendocladia bactrospora on Quercus variabilis in Korea

  • Lee, Dong-Hyeon;Seo, Sang-Tae;Lee, Sang-Hyun;Lee, Seung Kyu;Lee, Sun Keun
    • The Korean Journal of Mycology
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    • v.45 no.4
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    • pp.394-398
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    • 2017

  • A survey to assess the diversity of wound-associated Ophiostomatales and Microascales, the so-called ophiostomatoid fungi, on Korean native trees, was undertaken in 2017. Wounds were artificially created, and a fungus resembling a species of Sporendocladia was consistently isolated from the exposed cambium and inner bark of artificially induced wounds on Quercus variabilis. Morphological examination and DNA sequence comparisons based on the internal transcribed spacer (ITS) and 5.8S regions of the rDNA confirmed the identity of the fungus as Sporendocladia bactrospora. This is the first report on S. bactrospora occurring on Q. variabilis in Korea.

  • https://doi.org/10.4489/KJM.20170047 인용 PDF KSCI

Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat

  • Xie, Li-hua;Quan, Xin;Zhang, Jie;Yang, Yan-yan;Sun, Run-hong;Xia, Ming-cong;Xue, Bao-guo;Wu, Chao;Han, Xiao-yun;Xue, Ya-nan;Yang, Li-rong
    • The Plant Pathology Journal
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    • v.35 no.1
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    • pp.11-18
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    • 2019

  • Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

  • https://doi.org/10.5423/PPJ.OA.03.2018.0038 인용 PDF KSCI HTML

A Novel Protein Elicitor PeBL2, from Brevibacillus laterosporus A60, Induces Systemic Resistance against Botrytis cinerea in Tobacco Plant

  • Jatoi, Ghulam Hussain;Lihua, Guo;Xiufen, Yang;Gadhi, Muswar Ali;Keerio, Azhar Uddin;Abdulle, Yusuf Ali;Qiu, Dewen
    • The Plant Pathology Journal
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    • v.35 no.3
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    • pp.208-218
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    • 2019

  • Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

  • https://doi.org/10.5423/PPJ.OA.11.2018.0276 인용 PDF KSCI HTML