• The Plant Pathology Journal
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• 제38권4호
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• pp.334-344
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• 2022

Bacterial wilt is a re-emerging disease on dry bean and can affect many other crop species within the Fabaceae. The causal agent, Curtobacterium flaccumfaciens pv. flaccumfaciens (CFF), is a small, Gram-positive, rod-shaped bacterium that is seed-transmitted. Infections in the host become systemic, leading to wilting and economic loss. Clean seed programs and bactericidal seed treatments are two critical management tools. This study characterizes the efficacies of five bactericidal chemicals against CFF. It was hypothesized that this bacterium was capable of forming biofilms, and that the cells within biofilms would be more tolerant to bactericidal treatments. The minimum biocide eradication concentration assay protocol was used to grow CFF biofilms, expose the biofilms to bactericides, and enumerate survivors compared to a non-treated control (water). Streptomycin and oxysilver bisulfate had EC95 values at the lowest concentrations and are likely the best candidates for seed treatment products for controlling seed-borne bacterial wilt of bean. The results showed that CFF formed biofilms during at least two phases of the bacterial wilt disease cycle, and the biofilms were much more difficult to eradicate than their planktonic counterparts. Overall, biofilm formation by CFF is an important part of the bacterial wilt disease cycle in dry edible bean and antibiofilm bactericides such as streptomycin and oxysilver bisulfate may be best suited for use in disease management.

• The Plant Pathology Journal
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• 제21권4호
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• pp.395-401
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• 2005

The dlm (disease lesion mimic) mutant of soybean (Glycine max L. Merr) shows the similar lesion of a soybean disease caused by a fungus, Corynespora cassilcola. The lesion was examined at cellular and molecular level. Trypan blue staining result indicated that cell death was detectable in the entire region of leaves excluding veins when the lesions had already been developed. We found that the mesophyll cells of palisade layer in the dim mutant appeared to be wider apart from each other. The chloroplasts of the dim mutant cells contained bigger starch granules than those in normal plants. We also found that the lesion development of dlm plant was light-dependent and the starch degradation during the dark period of diurnal cycle was impaired in the mutant. Three soybean pathogenesis-related genes, PR-1a, PR-4, and PR-10, were examined for their expression patterns during the development of disease lesion mimic. The expression of all three genes was up-regulated to some extent upon the appearance of the disease lesion mimic. Although the exact function of DLM protein remains elusive, our data would provide some insight into mechanism underling the cell death associated with the dim mutation.

• 한국전자통신학회논문지
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• 제8권5호
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• pp.745-750
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• 2013

최근 수행된 많은 연구에서 시설 하우스나 식물 공장과 같이 실용적인 원예 시설에 대한 환경 제어 시스템이 다양하게 제시되었다. 그러나 아직까지도 식물의 전 생장 과정에 따른 온 습도 등 제어가 제대로 되지 않아 성장 장해 및 병충해에 노출되어 농가의 적지 않은 피해가 보고되고 있다. 공기 순환팬, 산업용 제습기 등을 활용하여 대책을 마련해 보고 있지만, 기대에 미치지 못하고 있다. 본 논문에서는 주기별 생장 인식 알고리즘을 이용하여 각 식물의 성장 단계를 인식하고 식물의 성장 단계에 따른 최적의 환경을 제공한다. 주기별 식물 생장 인식 자동 제어 모니터링 시스템을 이용하면 식물의 생장에 필요한 최적 환경을 제공하므로 생산성을 높일 수 있다.

• 산업융합연구
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• 제21권2호
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• pp.111-116
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• 2023

최근 바이러스 감염병 및 새로운 질병은 한 지역에 국한되는 것이 아니라 전 세계적으로 확산되어 경제적, 사회적으로 심각한 피해를 일으키고 있다. 또한, 새로운 질병의 발현 주기가 짧고, 확산 속도도 빨라지고 있다. 질병에 대한 확산을 막기 위해 격리, 폐쇄 등 질병 발생 후 수동적인 형태의 대응이 우선시 되고 있다. 이러한 형태의 대응은 질병 확산 방지에 근본적인 대응으로는 부족한 부분이 많이 있다. 이에, 본 연구에서는 새로운 질병 발생 정보를 포함한 질병 예방 모니터링 시스템을 제안한다. 본 연구에서는 IoMT 환경 구축을 통해 질병 정보와 사용자의 정보를 수집한다. devices를 이용한 정보 수집은 사용자의 생체정보와 질병 서버에 등록된 데이터를 수집하고 분류한다. IoMT 환경에서는 사용자의 데이터를 수집하여 사용자 질병 감염 여부를 평가하여 사용자에게 제공한다. 본 연구를 통해 개인의 질병 증상 정보를 제공하고, 질병 확산에 대한 능동적인 대응 방법을 제공할 수 있다.

• 생명과학회지
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• 제33권10호
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• pp.767-775
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• 2023

설파살라진은 1941년 최초로 합성된 이후, 류마티스 관절염, 궤양성 대장염 및 크론병을 치료하는 데 사용되는 질병 변형 항 류마티스 약물-비 생물제제 (아미노살리실산 유도체)이다. 1950년 미국에서 의약품으로 승인된 이후 다양한 염증성 질환의 치료제로 사용되고 있으나 이 약물의 작용기전은 아직 명확하게 밝혀지지 않고 있다. 본 연구에서는 설파살라진이 염증반응을 조절하는 주요 면역세포인 대식세포의 세포생존, 세포사멸 및 세포주기 진행에 어떠한 영향을 미치는지를 마우스 대식세포인 RAW264.7 세포를 이용하여 조사하였다. 세포생존에 미치는 설파살라진의 영향을 측정한 결과 농도의존적으로 RAW264.7 세포의 생존을 억제하였다. 이러한 세포생존의 억제가 세포사멸에 기인한 것인지를 확인하기 위해 Annexin-V로 염색한 결과 0.25 mM 이상의 농도에서 Annexin-V 양성세포가 유의적으로 증가하였다. 또한 0.25 mM 이상의 농도에서 G0/G1기에서 유의적으로 세포주기 정체를 유도하는 것을 확인할 수 있었다. G0/G1기를 조절하는 주요 단백질의 발현을 확인한 결과 설라살라진의 처리는 RAW264.7 세포에서 CDK의 억제제인 p21과 p27의 단백질 발현을 유의적으로 증가시켜 설라살라진에 의한 G0/G1기의 정체는 p21과 p27에 기인하는 것으로 사료된다. 염증성 궤장염이나 류마티스 관절염과 같은 염증성 질환에서 설라살라진이 대조약으로 빈번하게 사용되어지고 있음에도 불구하고 대식세포에 미치는 영향에 대한 연구가 매우 제한적이어서 본 연구의 결과가 질병치료제로서의 설파살라진의 이용에 기초적인 정보를 제공할 수 있을 것이라 판단된다.

• 동의신경정신과학회지
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• 제11권2호
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• pp.201-214
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• 2000

1. Essence and Spirit of association go well embodiment of mind goes well.2. Being component part by Three Valuable Properties of human body that is Essence, Vital force and Spirit, Cause that disease results is something wrong of Essence, Vital force and Spirit.3. Essence. Vital force and Spirit cycle that see in Doga is that circulate through intermediate of saliva.4. Morality curative means getting stability of mind as removing greed and it is that disease moves backward then naturally.5. Fantasy and uneasiness about the future is cause of disease of mind.

• 보건교육건강증진학회지
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• 제22권3호
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• pp.157-171
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• 2005

The Korean Government has produced the Health Plan 2010 aimed at setting up healthy Korea objectives, policies on preventing chronic diseases, reshaping the country's health and medical infrastructure. The policy goal targets the people's healthy life expectancy at 75 by 2010, and includes healthy life practice measures including health education, health improvement services, and disease management measures, in achieving the objectives. Also, the plan provides life cycle-based health improvement and disease prevention services, as well as pushes ahead with projects with greater ripple effects in each area. To this end, the government is simultaneously pushing to operate an experts-centered health promotion committee and establishing the infrastructure including the augmentation of national health improvement funds. Through its Health Plan 2010, the Korean Government will exert efforts to achieve its policy objectives as addressed in the measures by enhancing the national potential health and providing systematic disease prevention services.

• 식물병과 농업
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• 제5권1호
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• pp.1-7
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• 1999

The grain rot of caused by Bukholderia glumae was fist reported in japan in 1955 and then reported in other countries as well as in Korea in 1986. The pathogen causes both seedling and grain rot of rice but it cannot attack any other parts of adult rice plant. Bacterial colonies grow slowly, and are circular and greyish white. The causal bacterium is Gram-negative and rod shape with 1-3 polar flagella, and produce a diffusible yellow-greenish nonfluorescent pigment on King's medium B. Biochemical characteristics such as negative in arginine dehydrolase, oxidase reaction and nitrate reduction and positive in lecithinase, and the utilization of L-arginine and inositol are useful in differentiation of this from other nonfluorescent bacteria pathogenic to rice. This pathogenic bacterium had belonged to the genus of Pseudomonas but recently was transferred to the new genus Burkholderia on the basis of physiological characteristics and DNA-DNA hybridization data. However, other characteristics such as colony heterogenicity or colonial variation after subcultures, phytotoxin, secreting antibiotics, and relationship between yellow greenish pigment production and pathogenicity need to be clarified more. To develop an effective control strategy for this disease, understanding of detailed life cycle of the disease and critical environmental factors affecting disease development is prerequisite. Although 5,435 ha of rice paddy in Korea was infested during 1998, there is no exact estimation of yield losses and distribution of the pathogen. The review will focus on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

• 식물병과 농업
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• 제5권2호
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• pp.69-76
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• 1999

Acidovorax avenae subsp. avenae is the causal pathogen of several hosts including oats corn foxtail millet wheatgrass sugarcane and rice. The pathogen is a seedborne pathogen of rice and known to occur widely in rice growing countries. The pathogen cause inhibition of germination brown stripe on the leaf curling of the leaf sheath and abnormal elongation of the mesocotyl of irce. Bacterial colonies grow slowly and are convex circular and creamy with tan to brown center. The causal baterium is Gram-negative and rod shape with a single polar flagellum Nonfluorescence poly-$\beta$-hydroxybutyrate accumulation and precipitate formation around the colony on the medium are useful in the differentiation of this bacterium from other subspecies of A. avenae as well as nonfluorescent bacteria pathogenic to rice. This bacterium has belonged to the genus of Psdeudomonas but recently was transferred to the new genus Acidovorax on the basis of bacteriological and molecular biological data. However the difference of biochemical characteristics protein profile of the cell and host range among strains should be more clarified. To develop an effective control strategy for this disease understanding of detailed life cycle of the disease ritical environmental factors affecting disease development on each host and relationship to grain discoloration of rice are prerequisite. Although the affected area has been world-widely reported there is on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

• BMB Reports
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• 제47권2호
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• pp.104-109
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• 2014

FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non-small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment.