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http://dx.doi.org/10.5423/PPJ.2005.21.4.395

The Phenotype of the Soybean Disease-Lesion Mimic (dlm) Mutant is Light-Dependent and Associated with Chloroplast Function  

Kim, Byo-Kyong (School of Life Sciences and Biotechnology, Korea University)
Kim, Young-Jin (School of Life Sciences and Biotechnology, Korea University)
Paek, Kyoung-Bee (School of Life Sciences and Biotechnology, Korea University)
Chung, Jong-Il (Division of Plant Resources and Environment, Gyeongsang National University)
Kim, Jeong-Kook (School of Life Sciences and Biotechnology, Korea University)
Publication Information
The Plant Pathology Journal / v.21, no.4, 2005 , pp. 395-401 More about this Journal
Abstract
The dlm (disease lesion mimic) mutant of soybean (Glycine max L. Merr) shows the similar lesion of a soybean disease caused by a fungus, Corynespora cassilcola. The lesion was examined at cellular and molecular level. Trypan blue staining result indicated that cell death was detectable in the entire region of leaves excluding veins when the lesions had already been developed. We found that the mesophyll cells of palisade layer in the dim mutant appeared to be wider apart from each other. The chloroplasts of the dim mutant cells contained bigger starch granules than those in normal plants. We also found that the lesion development of dlm plant was light-dependent and the starch degradation during the dark period of diurnal cycle was impaired in the mutant. Three soybean pathogenesis-related genes, PR-1a, PR-4, and PR-10, were examined for their expression patterns during the development of disease lesion mimic. The expression of all three genes was up-regulated to some extent upon the appearance of the disease lesion mimic. Although the exact function of DLM protein remains elusive, our data would provide some insight into mechanism underling the cell death associated with the dim mutation.
Keywords
cell death; chloroplast; defense; disease lesion mimic; soybean;
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1 Bowling, S. A., Clarke, J. D., Liu, Y., Klessig, D. F. and Dong, X. 1997. The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance. Plant Cell 9:1573-1584   DOI   ScienceOn
2 Broderson, P., Petersen, M., Pike, H. M., Olszak, B., Skov, S., O dum, S., Jorgensen, L. B., Brown, R. E. and Mundy, J. 2002. Knockout of Arabidopsis ACCELERATED-CELL-DEATH11 encoding a sphingosine transfer protein causes activation of programmed cell death and defense. Genes Dev. 16:490-502   DOI   ScienceOn
3 Buschges, R., Hollricher, K., Panstruga, R., Simons, G., Wolter, M., Frijters, A., van Daelen, R., van der Lee, T., Diergaarde, P., Groenendijk, J., Topsch, S., Vos, P., Salamini, F. and Schulze-Lefert, P. 1997. The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88:695-705   DOI   ScienceOn
4 Hengartner, M. O. 2000. The biochemistry of apoptosis. Nature 407:770-776   DOI   PUBMED   ScienceOn
5 Hippeli, S., Heiser, I. and Elstner, E. F. 1999. Activated oxygen and free oxygen radicals in pathology: new insights and analogies between animals and plants. Plant Physiol. Biochem. 37:167-178   DOI   ScienceOn
6 Ishikawa, A., Okamoto, H., Iwasaki, Y. and Asahi, T. 2001. A deficiency of coproprophyrinogen III oxidase causes lesion formation in Arabidopsis. Plant J. 27:89-99   DOI   ScienceOn
7 Kim, H. K., Jang, Y. H., Baek, I. S., Lee, J. H., Park, M. J., Chung, Y.-S., Chung, J.-I. and Kim, J.-K. 2005. Polymorphism and expression of isoflavone synthase genes from soybean cultivars. Mol. Cells. 19:67-73
8 Manzano, D., Fernandez-Busquets, X., Schaller, H., Gonzalez, V., Boronat, A., Arro, M. and Ferrer, A. 2004. The metabolic imbalance underlying lesion formation in Arabidopsis thaliana overexpressing farnesyl diphosphate synthase (isoform 1S) leads to oxidative stress and is triggered by the developmental decline of endogenous HMGR activity. Planta 219:982-992   DOI   ScienceOn
9 Mock, H. P., Keetman, U., Kruse, E., Rank, B. and Grimm, B. 1998. Defense responses to tetrapyrrole-induced oxidative stress in transgenic plants with reduced uroporphyrinogen decarboxylase or coproporphyrinogen oxidase activity. Plant Physiol. 116:107-116   DOI   ScienceOn
10 Mou, A., He, Y., Dai, Y., Liu, X. and Li, J. 2000. Deficiency in fatty acid synthase leads to premature cell death and dramatic alterations in plant morphology. Plant Cell 12:405-417   DOI   ScienceOn
11 Shirasu, K. and Schulze-Lefert, P. 2000. Regulators of cell death in disease resistance. Plant Mol. Biot. 44:371-385   DOI   ScienceOn
12 van Doorn, W. G. and Woltering, E. J. 2005. Many ways to exit? Cell death categories in plants. Trends Plant Sci. 10:117-122   DOI   ScienceOn
13 Weintraub, M. and Ragetli, H. W. 1964. An electron microscope study of tobacco mosaic virus lesions in Nicotiana glutinosa L. J. Cell Biol. 23:499-509   DOI   ScienceOn
14 Von Ahsen, O., Waterhouse, N. J., Kuwana, T., Newmeyer, D. D., and Green, D. R. 2000. The 'harmless' release of cytochrome c. Cell Death Diff. 7:1192-1199   DOI   ScienceOn
15 Park, C. Y., Heo, W. D., Yoo, J. H., Lee, J. H., Kim, M. C., Chun, H. C., Moon, B. C., Kim, I. H., Park, H. C., Choi, M. S., Ok, H. M., Cheong, M. S., Lee, S. M., Kim, H. S., Lee, K. H., Lim, C. O., Chung, W. S. and Moo, J. C. 2004. Pathogenesis-related gene expression by specific calmodulin isoforms is dependent on NIM1, a key regulator of systemic acquired resistance. Mol. Cells 18:207-213
16 Shin, B. S., Lee, J. -H., Lee, J. -H., Jeong, H. -J., Yun, C. -H. and Kim, J. -K. 2004. Circadian regulation of rice (Oryza sativa L.) CONSTANS-like gene transcripts. Mol. Cells 17:10-16
17 Rate, D. N., Cuenca, J. V., Bowman, G. R., Guttman, D. S. and Greenberg, J. T. 1999. The gain-of-function Arabidopsis acd6 mutant reveals novel regulation and function of the salicylic acid signaling pathway in controlling cell death, defenses, and cell growth. Plant Cell 11:1695-1708   DOI   ScienceOn
18 Chung, J., Staswick, P. E., Graef, G. L., Wysong, D. S. and Specht, J. E. 1998. Inheritance of a disease lesion mimic mutant in soybean. J. Heredity 89:363-365   DOI   ScienceOn
19 Clarke, J. D., Liu, Y., Klessig, D. F. and Dong, X. 1998. Uncoupling PR gene expression from NPR1 and bacterial resistance: characterization of the dominant Arabidopsis cpr6-1 mutant. Plant Cell 10:557-569   DOI   ScienceOn
20 Stone, J. M., Heard, J. E., Asai, T. and Ausubel, F. M. 2000. Simulation of fungal-mediated cell death by fumonisin B1 and selection of fumonisin B1-resistant (fbr) Arabidopsis mutants. Plant Cell 12:1811-1822   DOI   ScienceOn
21 Mach, J. M., Castillo, A. R., Hoogstraten, R. and Greenberg, J. T. 2001. The Arabidopsis-accelerated cell death gene ACD2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms. Proc. Natl. Acad. Sci. USA 98:771-776   DOI   ScienceOn
22 Devoto, A., Piffanelli, P., Nilsson, I., Wallin, E., Panstruga, R., von Heijne, G. and Schulze-Lefert, P. 1999. Topology, subcellular localization, and sequence diversity of the Mlo family in plants, J. Biol. Chem. 274:34993-35004   DOI   ScienceOn
23 Greenberg, J. T. 1997. Programmed cell death in plant-pathogen interactions. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:525-545   DOI   PUBMED   ScienceOn
24 Hu, G., Richter, T. E., Hulbert, S. H. and Pryor, T. 1996. Disease lesion mimicry caused by mutations in the rust resistance gene rp1. Plant Cell 8:1367-1376   DOI   ScienceOn
25 Petersen, M., Brodersen, P., Naested, H., Andreasson, E., Lindhart, U., Johansen, B., Nielsen, H. B., Lacy, M., Austin, M. J., Parker, J. E., Sharma, S. B., Klessig, D. F., Martienssen, R., Mattsson O., Jensen, A. B. and Mundy, J. 2000. Arabidopsis map kinase 4 negatively regulates systemic acquired resistance. Cell 103:1111-1120   DOI   ScienceOn
26 Jones, A. M. 2001. Programmed cell death in development and defense. Plant Physiol. 125:94-97   DOI   PUBMED   ScienceOn
27 Goodman, R. N. and Novacky, A. J. 1994, The Hypersensitive Response Reaction in Plants to Pathogens, A Resistance Phenomenon. American Phytopathological Society Press, St Paul, Minnesota
28 Bowling, S. A., Guo, A., Cao, H., Gordon, A. S., Klessig, D. F. and Dong, X. 1994. A mutation in Arabidopsis that leads to constitutive expression of systemic acquired resistance. Plant Cell 6:1845-1857   DOI   ScienceOn
29 Hu, G., Yalpani, N., Briggs, S. P. and Johal, G. S. 1998. A porphyrin pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize. Plant Cell 10:1095-1105   DOI   ScienceOn
30 Jabs, T., Dietrich, R. A. and Dangl, J. L. 1996. Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide. Science 273:1853-1856   DOI   PUBMED   ScienceOn
31 Kim, Y. J. and Martin, G. B. 2004. Molecular mechanisms involved in bacterial speck disease resistance of tomato. Plant Pathol. J. 20:7-12   과학기술학회마을   DOI   ScienceOn
32 Lorrain, S., Vailleau, F., Balague, C. and Roby, D. 2003. Lesion mimic mutants: keys for deciphering cell death and defense pathways in plants? Trends Plant Sci. 8:263-271   DOI   ScienceOn
33 Molina, A., Volrath, S., Guyer, D., Maleck, K., Ryals, J. and Ward, E. 1999. Inhibition of protoporphyrinogen oxidase expression in Arabidopsis causes a lesion-mimic phenotype that induces systemic acquired resistance. Plant J. 17:667-678   DOI   ScienceOn
34 Collins, N., Drake, J., Ayliffe, M., Sun, Q., Ellis, J., Hulbert, S. and Pryor, T. 1999. Molecular characterization of the maize Rp1-D rust resistance haplotype and its mutants. Plant Cell 11:1365-1376   DOI   ScienceOn
35 Vogel, J. and Somerville, S. 2000. Isolation and characterization of powdery mildew-resistant Arabidopsis mutants. Proc. Natl. Acad. Sci. USA 97:1897-1902
36 Frye, C. A., Tang, D. and Innes, R. W. 2001. Negative regulation of defense responses in plants by a conserved MAPKK kinase. Proc. Natl. Acad. Sci. USA 98:373-378   DOI   ScienceOn
37 Mock. H. P., Heller, W., Molina, A., Neubohn, B., Sandermann, H. Jr. and Grimm, B. 1999. Expression of uroporphyrinogen decarboxylase or coproporphyrinogen oxidase antisense RNA in tobacco induces pathogen defense responses conferring increased resistance to tobacco mosaic virus. J. Biol. Chem. 274:4231-4238   DOI   ScienceOn
38 Simon, H-U., Haj-Yehia, A. and Levi-Schaffer, F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis 5:415-418   DOI   ScienceOn
39 Kliebenstein, D. J., Dietrich, R. A., Martin, A. C., Last, R. L. and Dangl, J. L. 1999. LSD1 regulates salicylic acid induction of copper zinc superoxide dismutase in Arabidopsis thaliana. Mol. Plant-Microbe Interact. 12:1022-1026   DOI   ScienceOn
40 Athow, K. L. 1987. Fungal diseases. In: Soybeans-improvement, production, and uses, ed. by J. R. Wilcox, pp. 687-727. American Society of Agronomy, Madison, Wisconsin
41 Sun, Q., Collins, N. C., Ayliffe, M., Smith, S. M., Drake, J., Pryor, T. and Hulbert, S. H. 2001. Recombination between paralogues at the rp1 rust resistance locus in maize. Genetics 158:423-438
42 Jabs, T. 1999. Reactive oxygen intermediates as mediators of programmed cell death in plants and animals. Biochem. Pharmacol. 57:231-245   DOI   PUBMED   ScienceOn