• Parasites, Hosts and Diseases
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• v.28 no.2
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• pp.101-108
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• 1990

Surgically collected cystic fluid of Taenia solium metacestodes from patients of intracranial cystic lesion were compared in their protein composition with those from naturally infected pigs in Cheju Do, Korea and Ecuador. In non-denaturing discontinuous-polyacryla aide gel electrophoresis (disc-PAGE) , no discernible differences were recognized in banding patterns between the cystic fluids from Cheju Do and Ecuador, and between the cystic quids from pigs and human lesions except wider bands that corresponded to human albumin and T-globulin (in 4 of 9 patients). In reducing SDS-PAGE, bands in the cystic Ruid from Ecuador showed the same banding pattern with that from Cheju Do but two bands of 21 and 17 kDa were stained darker. Cystic quids (rom patients revealed the same protein compositions of the major protein bands of 94, 64, 15, 10 and 7 kDa as in the cystic fluid of pig origin, but human albumin (66 kDa), heavy and light chains of gamma globulin (55 and 22.5 kDa) were contaminated in 4 of 9 cystic fluids. Human CSF proteins seem to have been contaminated during cystic ftuid collection. In any cystic quid from patients, the majcr Protein component was 150 kDa which was subdivided into 15, 10 and 7 kDa in reducing SDS-PAGE.

• YAKHAK HOEJI
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• v.30 no.6
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• pp.343-347
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• 1986

Korean ginseng was purified to obtain radioprotective protein fractions by buffer extraction, ammonium sulfate fractionation, CM-cellulose column chromatography, heat inactivation and Sephadex G-75 column chromatography. The final three fractions, GI, GII and GIII were subjected to Disc-polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. The molecular weights(M.W.) of native and denatured proteins were estimated by using regression line equations obtained from the mobilities of standard proteins. As the results, in Disc-PAGE, the GI fraction showed two protein bands with M.W. of above 213, 000 and 55, 000, GII showed one band with M.W. of 44, 000 and GIII, also one band with M.W. of 19, 000. In SDS-PAGE, GI fraction gave four subunit bands with M.W. of above 114, 000, 27, 000, 24, 000 and 19, 000, GII gave two bands with M.W. of 46, 000 and 22, 000, and GIII, one band of 19, 000.

• Clinical and Experimental Reproductive Medicine
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• v.13 no.1
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• pp.39-51
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• 1986

This experiment has been done to evaluate the relationship between the follicular atresia and the protein patterns on electrophoreais of the follicular fluids in porcine ovary. The protein concentration of the follicular fluids was lower than that of serum, and gradually decreased as the follicle siae became larger. The number of protein bands of follicular fluid on electrophoresis was less than that of serum, and gradually increased as follicle size became larger. Three specific bands were detected on disc PAGE and one band(M W. 75,000) on SDS PAGE in the follicular fluids, while not in serum. One band (A) at ${\beta}$-globulin region on disc PAGE became heavier, as follicles became atretic. Two bands less than(M. W. 20,000) were detected only in the large follicular fluid. Another band(M. W. 43,000) was not detected in necrotic group, whereas all other groups showed it. It could be concluded that the component and composition of the proteins follicular fluids changes according to the follicular size during atresia. Therefore detection of the changing pattern of proteins in the follicular fluid can be used as a basic criterion for the identification of follicular atretic stage.

• Parasites, Hosts and Diseases
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• v.29 no.4
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• pp.363-370
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• 1991

인체감염이 다발하는 폐흡충증의 혈청학적 진단에서 항원으로 사용하는 성충추출액은 여러단계의 질환 이행과정을 진단하는 항원으로서 문제가 있다. 이를 해결하려면 먼저 추출액내의 성분단백질의 성상을 파악할 필요가 있다. 이 연구에서는 폐흡충 성충 추출액으로 면역시킨 BALB/c mice의 비장세포와 SP2/0 형질세포종 세포를 세포융합하여 제작한 단세포군항체를 이용하여 친화성크로마토그래피로 폐흡충 성충의 구성단백질의 일부를 순수분리하고 성상을 관찰하였다. 그 결과는 다음과 같다. 1. 세포융합으로 PFCK-21, PFCK-44, PFCK-136, PFCK-189 등 4종류의 단세포군 항체를 얻었다. 그중 PFCK-21과 PFCK44는 17k Da, PFCK-136은 23, 46, 92 kDa 단백질에 반응하였고 PFCK-189는 여러종류의 단백질에 반응하였다. 2. PFCK-44 단세포군항체를 고리로 친화성 크로마토그래피를 실시하여 분리한 성분 단백질은 disc-PAGE상 4번째에 위치하고 분자량이 17 kDa로 알려진 단밸질이었다. 이 단밸질은 17 kDa의 monomer로 판단하였다. 면역조직화학염색을 실시한 결과 이 단밸질은 장관 상피세포에 반응하고 있었다. 3. PFCK-136 단세포군항체로 순수분리한 단밸질은 disc-PAGE상 1번 단밸질(440 kDa)이었으며 환원성 SDS-PAGE에서는 23 kDa 단밸질이었다. 면역조직화학염색으로 이 단세포군항체는 충란내 세포에 강하게 반응하고 있었다.

• Transactions of the Society of Information Storage Systems
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• v.7 no.1
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• pp.19-24
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• 2011

Holographic Data Storage System, one of the next generation data storage devices, is a 2-dimensional page oriented memory system using volume holograms in writing and retrieving process. Recently photopolymer with disc type substrate was selected as a media for the Holographic Data Storage System. The disc tilt occurs when the media rotates and the external disturbance applies. The disc tilt causes the change of the angle between the reference beam and the media, the data cannot be retrieved with the right angle or other data page is retrieved. The tilt is generated in a 2-axis direction (tangential, radial). The tangential tilt direction is the same with the multiplexing plane, while the radial tilt direction is a perpendicular to the multiplexing plane. In this research we propose 2-axis tilt angle servo system. The tilt errors are measured by using external photo detector and the additional red laser. Then the tangential direction tilt is compensated by using the galvano mirror. Also the radial direction tilt is compensated by the rotating prism between the relay lens in the reference field. Finally we confirm the compensation results through the Signal to Noise Ratio(SNR) and Bit Error Rate(BER).

• The Korean Journal of Food And Nutrition
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• v.6 no.4
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• pp.307-313
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• 1993

Bacillus cereus $\beta$-amylase was purified by Sephadex G-100 gel filtration, CM Sephadex C-50 ion exchange chromatography and CM Sephadex C-50 ion exchange rechromatography The purified enzyme showed 871 unit/mg of specific activity. The purified enzyme was identified as homogenious by disc PAGE, SDS-PAGE and analysis of reaction product. The purified enzyme showed optimum pH 7.0. optimum temperature 5$0^{\circ}C$, and was stable at 0~5$0^{\circ}C$ and at pH range of 6~10.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.373-378
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• 1998

This study was performed to investigate antimicrobial effects and their actions of the ethanol extracts of 12 spices against Lactobacillus plantarum and Leuconostoc mesenteroides, which are related to Kimchi fermentation. The cardamon, thyme, and cumin ethanol extracts among 12 spices showed antimicrobial activities against Lac. plantarum, not against Leu. mesenteroides through paper disc method. The growth of Lac. plantarum was inhibited in MRS broth containing each extracts of cardamon (>4%), thyme (>4%) and cumin (>1%). SDS-PAGE and transmission electron micrographs showed that the cell walls and membranes were disrupted and the cytoplasmic components were leaked in strains treated with ethanol extracts.

• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.87-94
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• 1988

By affinity chromatography using a monoclonal antibody as ligand, Kim et at. (1986) purified a protein fraction in cystic fluid of Taenia solium metacestodes (CF) In this study, the biochemical properties of the purified protein were characterized. Discontinuous-polyacrylamide gel electrophoresis (disc-PAGE) of the protein at 4.5∼10% separating gel concentration showed its molecular weight (MW) to be 150 kilodalton (kDa) in non·denatured state, while denaturing sodium dodecyl suifate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that it was composed of 3 different subunits with respective fnw of 15, 10 and 7 kDa. Subunit of 7 kDa was shown to be linked to other subunits by disulade bonds. Isoelectric point of the protein was pH 6.8. The protein was relatively heat-stable for immunologic analysis. These properties indicated that the protein, comprising about 70% of total content in CF, had similar biochemical characters with antigen B of Oriol et at.(1971) in hydatid cyst quid (HF).

• The Journal of the Korean dental association
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• v.22 no.12 s.187
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• pp.1083-1089
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• 1984

As a study to elucidate whether taste buds contain specific proteins, rat dorsal lingual epithelium was analysed by electrophoresis. The epithelium of the vallate papilla (with numerous taste buds), the area of the fungiform papilla 9with a few taste buds), and the area between vallate papilla and large filiform papilla (not containing taste buds) were strippled off by treatment with 0.7% EDTA. The epithelial protein was extracted by 1% SDS and 1% Mercaptoethanol in 0.01M phosphate buffer (pH7.2). Extracts were analysed by disc SDS-PAGE. Because the patterns of protein composition from each site were similar with each other as a whole, it is concluded that taste buds do not contain specific protein detected by SDS-PAGE in adult rat. But a protein on M.W. 49000 which lies in the area of molecular weight of keratin molecules was found only in the epithelium containing taste buds. This results suggested that the epithelium containing taste buds differentiate dissimilarly to the epithelium not containing taste buds.

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.113-120
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• 1991

When the component proteins in crude saline extract of 13-week old adult Paragonimus westermani were observed by non-denaturing discontinuous-polyacrylamide gel electrophoresis (Disc-PAGE), 8 distinct bands were clearly recognized. Molecular weight (MW) of each band protein, numbered in sequence from cathodal side which appeared in 10% separating gel, was measured first by Ferguson plot utilizing different gel concentrations from 10% to 4.5%. MW of band 1 Protein (known as egg Protein) was 440 kDa. And MW of other band Proteins were: 386 kDa in band 2, 17.4 kDa in band 3, 17kDa in band 4, 14.3 kDa in band 5, 46 kDa in band 6, 38 kDa in band 7 and 23 kDa in band 8. When the proteins in the crude extract were separated into fractions by molecular sieve chromatography through 1.6 (Φ)×70cm sired Sephacryl 5-300 Superane column and revisualized by Disc-PAGE in 8% gel, the sequence of fluted proteins was band 1, band 2, band 6, band 7 and bands 3,4,5 and 8. This elusion profile confirmed MW of each band protein in the crude extract as measured by Ferguson plot.