• Title/Summary/Keyword: direct synthesis

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Imaging Single-mRNA Localization and Translation in Live Neurons

  • Lee, Byung Hun;Bae, Seong-Woo;Shim, Jaeyoun Jay;Park, Sung Young;Park, Hye Yoon
    • Molecules and Cells
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    • v.39 no.12
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    • pp.841-846
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    • 2016
  • Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.

Some case studies of hydrodynamic bearings in power plants in Japan

  • M Tanaka
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2003.11a
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    • pp.1-11
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    • 2003
  • The service reliability of power plants strongly depends on the excellent performance and integrity of hydrodynamic bearings. Consequently, the bearings must be properly designed so as to control vibration amplitudes of rotor due to mass unbalance in passing critical speeds and also suppress self-excited vibrations of rotor even over maximum rated speeds. Furthermore, the bearings must be designed so as to maintain required tribological performance even under severe operating conditions. However, various tribological troubles have been experienced in power plants in Japan. The actual troubles are analysed, focusing on not only direct mechanical causes but also specific bearing designs that surfaced the troubles. Furthermore human factors that decided such designs are also studied. The powerful database of troubles and analyses will contribute greatly to designing advanced power plants with enhanced service reliability in the future. To this end, trouble information should be disclosed, shared and transferred limitlessly. Cooperation of users of power plants is essential to making more advanced design specifications, because no one has easier access to operating and trouble information of power plants than users.

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Growth and Structural Characterization of Single Layer Dichalcogenide $MoS_2$

  • Hwang, Jae-Seok;Kang, Dae-Joon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.575-575
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    • 2012
  • Synthesis of novel two dimensional materials has gained tremendous attention recently as they are considered as alternative materials for replacing graphene that suffers from a lack of bandgap, a property that is essential for many applications. Single layer molybdenum disulfide ($MoS_2$) has a direct bandgap (1.8eV) that is promising for use in next-generation optoelectronics and energy harvesting devices. We have successfully grown high quality single layer $MoS_2$ by a facile vapor-solid transport route. As-grown single layer $MoS_2$ was carefully characterized by using X-ray diffraction, Raman spectroscopy, field emission scanning electron microscopy and electrical transport measurement. The results indicate that a high quality single layer $MoS_2$ can be successfully grown on silicon substrate. This may open up great opportunities for the exploration of novel nanoelectronic devices.

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A Study on the Hypotensive Action of Akebiae Lignum Ethanol Extract in Rabbits (목통(木通) Ethanol Extract의 혈압강하작용(血壓降下作用)에 관(關)한 연구(硏究))

  • Kang, Ho-Yun
    • Journal of Pharmaceutical Investigation
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    • v.9 no.2
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    • pp.22-30
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    • 1979
  • Effects of Akebiae Lignum, whose scientific name is Akebia quinata Decaisne, on the blood pressure were investigated with EtOH extract in whole and spinal rabbits. Akebia Lignum EtOH extract (AEE), when given intravenously, produced a fall in blood pressure not only in whole rabbit but also in spinal rabbit and AEE administered into a lateral cerebral ventricle of whole rabbit did not elicit a fall in blood pressure. The depressor responses of the whole rabbit to intravenous AEE were weakened by treatment of the animals with atropine and chlorisondamine but not by vagotominijation, phentolamine avil, and then the depressor action causing by AEE in the whole rabbit was not affected by pretreatment of physostigmine which is cholinesterase inhibitor and of hemicholinium which blocks acetylcholine synthesis by interfering with choline uptake in nerves. These observations suggest that the hypotensive action of AEE of which component is not affected by cholinesterase is due to direct action at parasympathetic receptor.

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Studies on the Synthesis of Bisphenol Sulfonate Polymers and Their Staining Resist Effect in the Dyeing of Nylon/cotton Union (I) (Bisphenol sulfone산계 고분자의 합성 및 나일론 오염방지성에 관한 연구 (I))

  • 최영주;송승종;윤남식
    • Textile Coloration and Finishing
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    • v.12 no.4
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    • pp.256-263
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    • 2000
  • A series of poly[3-methylene-3'-methylenesodiumbisulfite-bis(4-hydroxyphenyl) sulfone] (PMSBPS) was synthesized by the reaction of bis-(4-hydroxyphenyl)sulfone(BPS), formalin, and formaldehyde sodium bisulfite(FSB), and their effect on the staining of direct dye on nylon in the dyeing of nylon/cotton union fabric was investigated. PMSBPSes have good staining resist effect on nylon in the dyeing of nylon/cotton union. Prolonged reacting time between BPS and formalin is effective in improving the staining resist of final PMSBPS. Too many sulfonic acid groups than necessary in the PMSBPS increase the staining of nylon, presumably by increasing the solubility of PMSBPS. Staining resist effect of PMSBPS was evident in the measurement of color diffence of dyed goods.

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Synthesis of the photochromic and electrochromic dyes and their sensible properties (I) - Dyeing application with photochromic moiety to the fiber substrate- (광변색성 및 전기변색성 색소의 합성 및 특성(I) -광변색성 특성의 섬유소재에의 응용-)

  • Son Young-A;Park Young-Min;Kim Byung-Soon;Kim Sung-Hoon
    • Textile Coloration and Finishing
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    • v.18 no.2 s.87
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    • pp.24-30
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    • 2006
  • The photosensitive spiroxazine compound and the electrosensitive viologen compound were prepared to examine their practical application feasibilities and behaviors. These dyes represent corresponding chromism effects related to their own characteristics of the dye molecules. Thus, the prepared dyes were characterized and their absorption spectra were also investigated. Besides, an interest on direct spiroxazine exhaustion to the polyamide substrates and its photochromic effects within the fiber molecules were determined. The photochromic reaction on the substrates was clearly observed and its reversible decoloration behaviors responded.

Modification of C.I. Acid Red 57 Synthesis Process (C.I. Acid Red 57의 제조공정 개선에 관한 연구)

  • Chung, Sang Woo;Kim, Jae Pil
    • Textile Coloration and Finishing
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    • v.9 no.5
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    • pp.75-81
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    • 1997
  • o-Aminobenzene sulfone-N-ethylanilide, the diazo component of C.I. Acid Red 57, is currently being diazotised by the use of nitrosylsulphuric acid because of its high molecular weight and weak basicity. However, this method has many problems such as complicated manufacturing process and discharge of large volume of strong acidic effluent. In this study, the possibility of replacing nitrosylsulphuric acid method by direct method, which is simpler and produces less effluent, has been checked and the optimum process condition was studied. The effect of HCl concentration, the amount of sodium nitrite and particle size of diazo component on diazotisation yield was investigated. The optimum pH condition for coupling reaction was also evaluated.

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Synthesis of Poly(2,6-dimethyl-1,4-phenylene oxide) containing Poly(styrene sulfonic acid) for Fuel Cell (연료전지용 Poly(styrene sulfonic acid)를 갖는 Poly(2,6-dimethyl-1,4-phenylene oxid)의 합성)

  • An, Sung-Guk;Cho, Chang-Gi
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.10b
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    • pp.34-37
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    • 2003
  • The development and improvement of advanced fuel cell systems is a major topic of current research, since fuel cells are considerably more efficient than other energy converters$^1$. In proton exchange membrane fuel cell and direct methanol fuel cell the polymer membrane represents a key component. The membrane materials fulfill complex requirements. It has to combine electrochemical stability, workability, high ionic conductivity, low permeation of the reactants (methanol etc.) (omitted)

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The Synthesis of n-Dopecyl-Tetracyanoquinodimethane( I ) (n-Dopecyl-Tetracyanoquino-dimethane의 합성( I ))

  • Jeong, Soon-Wook;Park, Keun-Ho;Sohn, Byung-Chung;Nam, Ki-Dae;Lee, Won-Jae;Kang, Dou-Yol
    • Proceedings of the KIEE Conference
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    • 1987.11a
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    • pp.515-517
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    • 1987
  • The direct alleylation of 1,4-cyclohexanodione with 1-dodecene underwent in one stop and n-dodecyl-tetracyanoquinodimethano was synthesized. The total yield of n-dodecyl-tetracyunoquinodimethane was about 55.2% when the n-dodecyl-1,4-cyclohexanedione was a starting material.

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