Adikari Arachchige Dilki Indrachapa Adikari;Young-Joo Yi
Korean Journal of Agricultural Science
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v.49
no.2
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pp.307-316
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2022
The misuse of pesticides has resulted in environmental pollution, which directly or indirectly affects all life on earth. Chlorpyrifos is a chlorinated organophosphorus pesticide that is commonly used in agriculture. The aim of this study was to investigate the effects of chlorpyrifos on the fertilization function of boar spermatozoa. Sperm samples from boars were subjected to varying concentrations of chlorpyrifos from 10 to 200 µM for two incubation periods, 30 min or 2 hrs. The boar spermatozoa were then evaluated for motility, motion kinematics, viability, acrosome integrity, chromatin stability, and generation of intracellular reactive oxygen species (ROS). There was a significant percentage reduction in sperm motility and motion kinematic parameters after both incubation periods (p < 0.05). The proportion of viable spermatozoa decreased after incubation for 30 min and 2 hrs in a dose-dependent manner (p < 0.05). A significantly lower percentage of normal acrosomes was observed in spermatozoa exposed to 200 µM chlorpyrifos over both incubation periods, compared to the controls. The damage to sperm DNA was significantly higher when the exposure time to chlorpyrifos was longer. There was a significant increase in the ROS levels in spermatozoa incubated with chlorpyrifos for 2 hrs (p < 0.05). From the results of the present study, it is concluded that direct exposure of boar spermatozoa to chlorpyrifos altered boar sperm characteristics, suggesting potential toxicity that may affect the male reproductive function.
The nature of distant faint blue field galaxies remains a mystery, despite the fact that much attention has been devoted to this subject in the last decade. Galaxy counts, particularly those in the optical and near ultraviolet bandpasses, have been demonstrated to be well in excess of those expected in the 'no-evolution' scenario. This has usually been taken to imply that galaxies were brighter in the past, presumably due to a higher rate of star formation. More recently, redshift surveys of galaxies as faint as B$\~$24 have shown that the mean redshift of faint blue galaxies is lower than that predicted by standard evolutionary models (de-signed to fit the galaxy counts). The galaxy number count data and redshift data suggest that evolutionary effects are most prominent at the faint end of the galaxy luminosity function. While these data constrain the form of evolution of the overall luminosity function, they do not constrain evolution in individual galaxies. We are carrying out a series of observations as part of a long-term program aimed at a better understanding of the nature and amount of luminosity evolution in individual galaxies. Our study uses the luminosity-linewidth relation (Tully-Fisher relation) for disk galaxies as a tool to study luminosity evolution. Several studies of a related nature are being carried out by other groups. A specific experiment to test a 'no-evolution' hypothesis is presented here. We have used the AUTOFIB multifibre spectro-graph on the 4-metre Anglo-Australian Telescope (AAT) and the Rutgers Fabry-Perot imager on the Cerro Tolalo lnteramerican Observatory (CTIO) 4-metre tele-scope to measure the internal kinematics of a representative sample of faint blue field galaxies in the red-shift range z = 0.15-0.4. The emission line profiles of [OII] and [OIII] in a typical sample galaxy are significantly broader than the instrumental resolution (100-120 km $s^{-l}$), and it is possible to make a reliable de-termination of the linewidth. Detailed and realistic simulations based on the properties of nearby, low-luminosity spirals are used to convert the measured linewidth into an estimate of the characteristic rotation speed, making statistical corrections for the effects of inclination, non-uniform distribution of ionized gas, rotation curve shape, finite fibre aperture, etc.. The (corrected) mean characteristic rotation speed for our distant galaxy sample is compared to the mean rotation speed of local galaxies of comparable blue luminosity and colour. The typical galaxy in our distant sample has a B-band luminosity of about 0.25 L$\ast$ and a colour that corresponds to the Sb-Sd/Im range of Hub-ble types. Details of the AUTOFIB fibre spectroscopic study are described by Rix et al. (1996). Follow-up deep near infrared imaging with the 10-metre Keck tele-scope+ NIRC combination and high angular resolution imaging with the Hubble Space Telescope's WFPC2 are being used to determine the structural and orientation parameters of galaxies on an individual basis. This information is being combined with the spatially resolved CTIO Fabry-Perot data to study the internal kinematics of distant galaxies (Ing et al. 1996). The two main questions addressed by these (preliminary studies) are: 1. Do galaxies of a given luminosity and colour have the same characteristic rotation speed in the distant and local Universe? The distant galaxies in our AUTOFIB sample have a mean characteristic rotation speed of $\~$70 km $s^{-l}$ after correction for measurement bias (Fig. 1); this is inconsistent with the characteristic rotation speed of local galaxies of comparable photometric proper-ties (105 km $s^{-l}$) at the > $99\%$ significance level (Fig. 2). A straightforward explanation for this discrepancy is that faint blue galaxies were about 1-1.5 mag brighter (in the B band) at z $\~$ 0.25 than their present-day counterparts. 2. What is the nature of the internal kinematics of faint field galaxies? The linewidths of these faint galaxies appear to be dominated by the global disk rotation. The larger galaxies in our sample are about 2"-.5" in diameter so one can get direct insight into the nature of their internal velocity field from the $\~$ I" seeing CTIO Fabry-Perot data. A montage of Fabry-Perot data is shown in Fig. 3. The linewidths are too large (by. $5\sigma$) to be caused by turbulence in giant HII regions.
Three types of glass woven fabrics (plain, balanced twill, and unbalanced twill) having various sample sizes and aspect ratios were tested using the bias-extension tests. Real-time deformation images, force, and displacement data were collected. For the bias-extension test, the shear angle of the fabrics from the equation based on the crosshead displacement and fabric size was compared with direct manual measurements of the warp and weft angles as well as the optical measurement software. To determine the shear force, an analytical equation was introduced considering the kinematics of the bias-extension test. The obtained shear behaviors were further compared with the results by the trellis-frame test. The optical measurement methods showed that the mathematical method was reasonable before the shear angle of the fabrics reaches $30^{\circ}$ in the bias-extension tests. Also, the bias-extension test gave consistent behaviors with the trellis-frame test only for isotropic and homogeneous fabrics such as balanced plain and twill weaves.
A finite element model development of a 50th percentile male cervical spine is presented in this paper. The model consists of rigid, geometrically accurate vertebrae held together with deformable intervertibral disks, facet joints, and ligaments modeled as a series of nonlinear springs. These deformable structures were rigorously tuned, through failure, to mimic existing experimental data; first as functional unit characterizations at three cervical levels and then as a fully assembled c-spine using the experimental data from Duke University and other data in the NHTSA database. After obtaining satisfactory validation of the performance of the assembled ligamentous cervical spine against available experimental data, 22 cervical muscle pairs, representing the majority of the neck's musculature, were added to the model. Hill's muscle model was utilized to generate muscle forces within the assembled cervical model. The muscle activation level was assumed to be the same for all modeled muscles and the degree of activation was set to correctly predict available human volunteer experimental data from NBDL. The validated model is intended for use as a post processor of dummy measurement within the simulated injury monitor (SIMon) concept being developed by NHTSA where measured kinematics and kinetic data obtained from a dummy during a crash test will serve as the boundary conditions to "drive" the finite element model of the neck. The post-processor will then interrogate the model to determine whether any ligament have exceeded its known failure limit. The model will allow a direct assessment of potential injury, its degree and location thus eliminating the need for global correlates such as Nij.
This study is to analyze the kinematic variables of inward $1{\frac{1}{2}}$ somersault in platform diver. For the manner, 3 people form the national diving team in the year 2000were chosen as the subjects and two S-VHS video cameras set in 60frames/sec were used for recording their motions. Coordinated raw positions data through digitizing are smoothing by butter-worth's low-pass filterin method at a cut off frequency 6.0Hz. and the direct linear transformation(DLT) method was employed to obtain 3-D position coordinates. The conclusions were as follows. However, horizontal distance which is the change of the COG, form the point of the jump to the point of Event 3 where the player is out of the board range completely, Subject B showed 105.1cm and 71.1cm of the vertical distance which are shorter horizontal distance and higher vertical distance, thus, took a great advantage of the position to prepare for the entry. Therefore, if a player takes higher position by speeding up the vertical velocity at the moment of the jumping off the board, and stays in the air longer, the player can have more time to show his skill. Because of the use of the characteristics of the inward somersault, keeping the safe distance form the board is important but in order to higher the completeness, it is ideal to keep the horizontal distance little over 100cm. Also, the angles of shoulder and elbow from Event 1 to 4, depending on swing of the arms, motions in the air, getting ready for the entry, showed some difference individual by individual, according to the velocity of the thigh and shank showed much difference while getting ready and take-off, and it's because of the individual's different bending and straightening for horizontal and vertical distance.
The present work deals with second order statistics of post buckling response of piezoelectric laminated composite cylindrical shell panel subjected to hygro-thermo-electro-mechanical loading with random system properties. System parameters such as the material properties, thermal expansion coefficients and lamina plate thickness are assumed to be independent of the temperature and electric field and modeled as random variables. The piezoelectric material is used in the forms of layers surface bonded on the layers of laminated composite shell panel. The mathematical formulation is based on higher order shear deformation shell theory (HSDT) with von-Karman nonlinear kinematics. A efficient $C^0$ nonlinear finite element method based on direct iterative procedure in conjunction with a first order perturbation approach (FOPT) is developed for the implementation of the proposed problems in random environment and is employed to evaluate the second order statistics (mean and variance) of the post buckling load of piezoelectric laminated cylindrical shell panel. Typical numerical results are presented to examine the effect of various environmental conditions, amplitude ratios, electrical voltages, panel side to thickness ratios, aspect ratios, boundary conditions, curvature to side ratios, lamination schemes and types of loadings with random system properties. It is observed that the piezoelectric effect has a significant influence on the stochastic post buckling response of composite shell panel under various loading conditions and some new results are presented to demonstrate the applications of present work. The results obtained using the present solution approach is validated with those results available in the literature and also with independent Monte Carlo Simulation (MCS).
Objective: The aim of this study was to provide kinematic data on the characteristics of spinal disease patients by comparing and analyzing kinematic variables related to foot balance and gait pattern of spinal disease. Method: The subjects of the study included 40 adult men and 60 adult women who visited the hospital in Busan. Patients who were diagnosed with spinal disease by a physician through X-ray examination were selected as subjects for the diagnosis of vertebral disc herniation, spinal stenosis, spinal disease diagnosed with spinal disease and the general public. Left and right foot pressure and contact area were checked by Gaitview pro meter. X-ray photographs were taken with a Zen-2090 mobile fluoroscopy under physicians' direct participation. One-way ANOVA was performed to compare the differences between the kinematic variables and post-hoc was performed by the Duncan method. Results: The difference in contact area between the left foot and the right foot was $115.30{\pm}14.15cm^2$ in the left side and $124.25{\pm}13.65cm^2$ in the left side in the spinal disease patients. The difference in pressure between the left and right side of the spinal disease patients was wider than that of the general people. Especially, the right side of the spinal disease patients showed a larger area of left foot contact than the general population. Conclusion: Spinal disease patients have wider contact area of the left foot than those of the general population. In the case of right spinal disease, the left foot support area is widened due to pain. In the gait, women showed slightly more posterior body center than men, and the upper body muscle imbalance and immobilization due to the spinal disease caused imbalance of the muscles moving to the lower limb, It was analyzed to inhibit movement.
Wijesooriya Mudhiyanselage Nadeema Dissanayake;Jung Min Heo;Young-Joo Yi
Korean Journal of Agricultural Science
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v.50
no.4
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pp.941-952
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2023
The metabolites of agrichemicals, such as organophosphorus pesticides, are known to be more hazardous than their parent pesticides. 3,5,6-trichloro-2-pyridinol (TCP) is a major degradation product of chlorpyrifos, one of the organophosphate insecticides widely used in agriculture. In vivo or in vitro exposure to chlorpyrifos has been known to interfere with male reproductive functions, leading to reduced fertility in mammals. Therefore, this study was performed to examine the changes in the fertilization competence of boar spermatozoa exposed to TCP. Sperm samples were subjected to varying concentrations of TCP (10, 50, 100, 200 µM) and different periods of incubation. Sperm motility, motion kinematics, viability, acrosome integrity, intracellular reactive oxygen species (ROS) production, and gene expression levels (ODf2, ZPBP2, AKAP3 and AKAP4) were evaluated after exposure of the sperm to TCP. A significant dose-dependent reduction in motility was observed in sperm samples incubated with TCP compared to the controls after both incubation periods. Sperm viability was significantly decreased in samples incubated with 50, 100, and 200 µM TCP in both incubation periods. A significantly lower percentage of normal acrosomes and gene expression levels were observed in sperm samples exposed to 50, 100, and 200 µM TCP after both incubation periods, compared to the controls. There was a significant increase in the ROS production in spermatozoa incubated with 100 - 200 µM TCP after both incubation periods. Consequently, the direct exposure of boar spermatozoa to TCP interferes with sperm functions and leads to decreased fertilization. In order to identify and address the various causes of reproductive decline, the impact of chemical metabolites needs to be discussed in depth.
Journal of the Korean Society of Fisheries and Ocean Technology
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v.14
no.2
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pp.97-112
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1978
The rule 15, 16 and 17 of International Regulations for Preventing collisions at Sea direct actions to avoid collision when two power-driven vessels are crossing. But these rules do not present the safety minimum approaching distances outside which a give- way vessel deeps out of the way of a stand-on vessel. In this paper, the author analyzed the ship's collision avoiding actions from a viewpoint of ship kinematics as the method to calculate this distance. The author worked out mathematic formulas for calculating the safety minimum approaching distances outside which the give-way vessel takes the actions to avoid collisions in accordance with the cross angles of the crossing vessels' courses. Figuring out actually the values of maneuvering indices of the M. S. Koan Ack San (GT: 224tons), the training ship of the National Fisheries University of Busan and the M. S. Golden Clover (GT: 101, 235tons) of the Eastern Shipping Co., Ltd. through their Z test, the author applied these values to the calculating formulas and calculated the safety minimum approaching distances. The results of calculations are as follows; 1. The greatest distance is to be kept by the give-way vessel to avoid collision when the cross angle of courses is 90$^{\circ}$ or near it. In such case the safety minimum approaching distance of a small vessel must be more than 5 times of her own length and that of a large vessel more than 11 times of her own length. 2. Collision danger is greater when crossing angle is obtuse than in an acute angle, therefore greater distance is to be kept by the give-way vessel to avoid collision in the case of the obtuse angle. 3. The actions to be taken to avoid collisions by the give-way vessel in Rule 16 and by the stand-on vessel in Rule 17(a)(ii) of International Regulations for Preventing Collisions at Sea, must be done outside the above safety minimum approaching distance. When inevitably such actions are to be taken within the safety minimum approaching distance, they should be accompanied with engine motions.
The duplex system has been considered as an important slip-transfer mechanism to evaluate the evolution of orogenic belts. Duplexes are generally found in the hinterland portion of fold-thrust belts and accommodate large amounts of total shortening. Thus, understanding its geometric and kinematic evolution can give information to evaluate the evolution of the entire orogenic belt. Duplexes are recognized as closed-loop thrust traces on map view, indicating higher connectivity than imbricate fans. As originally defined, a duplex is an array of thrust horses which are surrounded by thrust faults including the floor and roof thrusts, and imbricate faults between them. Duplexes can accommodate regional layer-parallel shortening and transfer slip from a floor thrust to a roof thrust. However, an imbricate fault is not the only mean for layer-parallel shortening (LPS) and displacement transfer within duplexes. LPS cleavages and detachment folds can also play the same role. From this aspect, a duplex can be divided into three types; 1) fault duplex, 2) cleavage duplex and 3) fold duplex. Fault duplex can further be subdivided into the Boyer-type duplex, which was firstly designed duplex system in the 1980s that widely applied most of the major fold-thrust belts in the world, and connecting splay duplex, which has different time order in the emplacement of horses from those of the Boyer-type. On the contrary, the cleavage and fold duplexes are newly defined types based on some selected examples. In the Korean Peninsula, the Yeongwol area, the western part of the Taebaeksan Zone of the Okcheon Belt, gives an excellent natural laboratory to study the structural geometry and kinematics of the closed-loops by thrust fault traces in terms of a duplex system. In the previous study, the Yeongwol thrust system was interpreted by alternative duplex models; a Boyer-type hinterland-dipping duplex vs. a combination of major imbricate thrusts and their connecting splays. Although the high angled beds and thrusts as well as different stratigraphic packages within the horses of the Yeongwol duplex system may prefer the later complicate model, currently, we cannot choose one simple answer between the models because of the lack of direct field evidence and time information. Therefore, further researches on the structural field investigations and geochronological analyses in the Yeongwol and adjacent areas should be carried out to test the possibility of applying the fold and cleavage duplex models to the Yeongwol thrust system, and it will eventually provide clues to solve the enigma of formation and its evolution of the Okcheon Belt.
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