• Plant Biotechnology Reports
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• v.3 no.4
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• pp.333-340
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• 2009

Developmental anomalies in the plumule meristem of peanut (Arachis hypogaea L.) somatic embryos resulted in poor shoot differentiation and reduced plant recovery. Existing meristems with caulogenic potential have never been tested for embryogenesis in peanut. The present experiment was designed to test the mature zygotic embryo axis derived plumule with three meristems for somatic embryogenesis. Embryogenic masses and embryos developed from the caulogenic meristems in the axils. Exposure of 2 weeks in primary medium with $90.5{\mu}M$ 2,4-D suppressed the shoot tip differentiation temporarily which then regained the ability to form the shoot on withdrawal of 2,4-D. Exposure of 4 weeks in primary medium with $90.5{\mu}M$ 2,4-D suppressed the shoot tip differentiation irreversibly. No shoot formation was noted from the tips in any of the cultures which were in secondary medium with $13.6{\mu}M$ 2,4-D. Development of somatic embryos directly from axillary meristems was confirmed histologically. Conversion frequency of these embryos was 11%. Thus, in this report, we describe a method to obtain somatic embryos from the determined organogenic buds of the axillary meristem, by culturing the nodal explant vertically on embryo induction medium. It also displays the possibility of obtaining both embryogenic and organogenic potential in two parts of the same explant simultaneously. The possibility of extending this approach for genetic transformation in in vivo system through direct DNA delivery or Agrobacterium injection in meristems can also be explored. Using Agrobacterium rhizogenes, we have demonstrated the possibility of gene transfer in the axillary meristems of seed-derived plumule explant.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.3
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• pp.321-327
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• 2007

This study presents a new gridless finite difference method for solving elastic crack problems. The method constructs the Taylor expansion based on the MLS(Moving Least Squares) method and effectively calculates the approximation and its derivatives without differentiation process. Since no connectivity between nodes is required, the modeling of discontinuity embedded in the domain is very convenient and discontinuity effect due to crack is naturally implemented in the construction of difference equations. Direct discretization of the governing partial differential equations makes solution process faster than other numerical schemes using numerical integration. Numerical results for mode I and II crack problems demonstrates that the proposed method accurately and efficiently evaluates the stress intensity factors.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.3
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• pp.237-244
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• 2002

Analyzing acoustic-structural systems such as automobiles and aircraft, the FRF-based substructuring (FBS) method is one of the most powerful tools. In this paper, a general procedure for the parametric sensitivity analysis of vibro-acoustic problems has been presented using the multi-domain FRF-based substructuring formulation. For an acoustic-structural system sub-structured by multiple domains, the substructuring formulation gives the reaction farces on the interface boundaries. The design sensitivity formula is obtained from the direct differentiation of the reaction force expression with respect to the design vector. As a practical application, the proposed design sensitivity formula is applied to an engine mount system of passenger car. An objective of the problem is to identify the most effective engine mounts and bushes in minimizing the interior noise over the concerned rpm range. The comparison of the sensitivity results with those of the finite difference method shows excellent agreement. In addition, stiffness modifications of the mounts and bushes identified through the design sensitivity analysis lead to a successful decrease of the interior noise. This results show usefulness of the present method very well.

• Journal of Power System Engineering
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• v.5 no.4
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• pp.82-89
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• 2001

To design a structural or a mechanical system with the best performance, the main procedure of a typical design usually consists of repeated modifications of design parameters and the investigation of the system response for each set of these parameters. But this procedure requires much time, effort and experience. Sensitivity analysis can provide systematic information for improving performance of a system. The author has studied on the development of the structural analysis algorithm and suggested recently the transfer stiffness coefficient method(TSCM). This method is very suitable algorithm to a personal computer because the concept of the TSCM is based on the transfer of the nodal stiffness coefficients which are related to force and displacement vectors at each node. In this paper, a new sensitivity analysis algorithm using the concept of the TSCM is formulated for the computation of state variable sensitivity in static problems. The trust of the proposed algorithm is confirmed through the comparison with the computation results using existent sensitivity analysis algorithm and reanalysis for computation models.

• Journal of the Society of Naval Architects of Korea
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• v.36 no.2
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• pp.105-113
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• 1999

This paper resents a prediction method of natural frequencies of coupled horizontal and torsional vibration of hull girder based on design sensitivity analysis in case of the changes of system parameters. The sensitivity analysis is formulated applying the direct differentiation method and transfer matrix method. In the analysis, warping, shear deformation due to torsion and the continuity condition at the connected part of open and closed hull section are considered. Using the presented method. The affection for natural frequencies by the change of system parameters, especially cargo and added mass and their centers, is numerically investigated for a real large container carrier.

• International Journal of Concrete Structures and Materials
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• v.6 no.2
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• pp.101-110
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• 2012

This paper focuses on the finite element (FE) response sensitivity and reliability analyses considering smooth constitutive material models. A reinforced concrete frame is modeled for FE sensitivity analysis followed by direct differentiation method under both static and dynamic load cases. Later, the reliability analysis is performed to predict the seismic behavior of the frame. Displacement sensitivity discontinuities are observed along the pseudo-time axis using non-smooth concrete and reinforcing steel model under quasi-static loading. However, the smooth materials show continuity in response sensitivity at elastic to plastic transition points. The normalized sensitivity results are also used to measure the relative importance of the material parameters on the structural responses. In FE reliability analysis, the influence of smoothness behavior of reinforcing steel is carefully noticed. More efficient and reasonable reliability estimation can be achieved by using smooth material model compare with bilinear material constitutive model.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.15 no.2
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• pp.18-24
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• 2007

The high ionospheric spatial gradient during ionospheric storm is the most concern when applying GNSS(Global Navigation Satellite System) augmentation systems for aircraft precision approach. Since the ionospheric gradient level depends on geographical location as well as the storm, understanding the ionospheric gradient statistics over a specific regional area is necessary for operating the augmentation systems. This paper compares three ionosphere gradient computation methods, direct differentiation between two receivers' ionospheric delay signal for a common satellite, derivation from a grid ionosphere map, and derivation from a plate ionosphere map. The plate map method provides a good indication on the gradient variation behavior over a regional area with limited number of GNSS receivers. The residual analysis for the ionosphere storm detection is discussed as well.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.628-634
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• 1995

In the area of assembly process of micro-chips and electronic parts on the printed circuit board, surface mounting device(SMD) is used as a fundamental tool. Generally speaking, the motion of the SMD is based on the ball screw system operated by any type of actuators. The ball screw system is a mechanical transformer which converts the mechanical rotational motion to the translational one. Also, this system could be considered as an efficient motion device against mechanical backash and friction. Therefore a dynamic modeling and stste sensitivity analysis of the ball screw system in SMD have to be done in the initial design stage. In this paper, a simple mathematical dynamic model for this system and the sensitivity snalysis are mentioned. Especially, the bond graph approach is used for graphical modeling of the dynamic system before analysis stage. And the direct differentiation method is used for the state sensitivity analysis of the system. Finally, some trends for the state variables with respect to the design variables could be suggested for the better design based on the results on the results of dynamic and state sensitivity.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.05a
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• pp.1038-1043
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• 2003

There are many industrial applications including thin-body structures such as fins. For the numerical modeling of radiation of sound from thin bodies, the conventional boundary element method (BEM) using the Helmholtz integral equation fails to yield a reliable solution. Therefore, many researchers have tried to solve the thin-body acoustic problems. In the area of the design sensitivity analysis (DSA) and optimization methods, however, there has been just a few study reported. Especially fur the thin-body acoustics, however, no further study in the DSA and optimization fields has been reported. In this research, the normal derivative integral equation is adopted as an analysis formulation in the thin-body acoustics, and then used for the sizing DSA and optimization. Since the gradient-based method is used for the optimization, it is important to have accurate gradients (design sensitivities) of the objective function and constraints with respect to the design variables. The DSA formulations are derived through chain-ruled derivatives using the finite element method (FEM) and BEM by using the direct differentiation and continuum variation concepts. The proposed approaches are implemented and validated using a numerical example.

• Journal of Korean Society of Forest Science
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• v.3 no.1
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• pp.28-31
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• 1963

Recognizing shortcomings and unreasonable points of the usual methods of measurement of the standing-tree volume, author contrived measurement formula of standing-tree volume which is based on stem-curve equation, $y^2=2px$, resulted from the analysises and experiments on stem-curve by differentiation & integration. The results of this study are as follows ; (1) The following is the formula. $V=1/2g_{1.2}$(h+1.2) V=volume of tree $g_{1.2}$ = sectional area at breast heigh h = height of tree (2) So plain is the form of this formula that this formula is more convenient than usual formulas in measuring. (3) The percentage error of this formula is negative and it is remarkably superior to usual formulas except Pressler's formula, in other words, the higher the tree that is measured is, the more inaccurate become pressler's formula, and the percentage error of the breast-height form factor method is direct proportion to the grossness of the tree that is measured, but the higher the tree that is measured, the lower become percentage error of this formula in geometrical progression.