• 한국발생생물학회지:발생과생식
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• 제17권1호
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• pp.1-8
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• 2013

Osteoprotegerin (OPG) is a secreted glycoprotein that regulates bone resorption by inhibiting differentiation and activation of osteoclast, thereby potentially useful for the treatment of many bone diseases associated with increased bone loss. In this study, we designed a novel cDNA expression cassette by modifying the potent and mammary gland-specific goat ${\beta}$-casein/hGH hybrid gene construct and examined human OPG (hOPG) cDNA expression in transgenic mice. Six transgenic mice all successfully expressed hOPG in their milk at the level of 0.06-2,000 ${\mu}g/ml$. An estimated molecular weight of the milk hOPG was 55 kDa in SDS-PAGE, which is the same as a naturally glycosylated monomer. This hOPG expression was highly specific to the mammary glands of transgenic mice. hOPG mRNA was not detected in any organs analyzed except mammary gland. Functional integrity of milk hOPG was evaluated by TRAP (tartrate-resistant acid phosphatase) activity assay in bone marrow cell cultures. OPG ligand (OPG-L) treatment increased TRAP activity by two fold but it was completely abolished by co-treatment with transgenic milk containing hOPG. Taken together, our novel cDNA expression cassette could direct an efficient expression of biologically active hOPG, a potential candidate pharmaceutical for bone diseases, only in the mammary gland of transgenic mice.

• Biomolecules & Therapeutics
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• 제6권3호
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• pp.276-282
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• 1998

Retinoids regulate a wide variety of biological processes such as cellular proliferation and differentiation in many cell types. They have also shown to stimulate replication of several viruses including human cytomegalovirus (CMV). Retinoid signalling pathway involves two distinct subfamilies of nuclear receptors, retinoic acid receptors (RARs) and retinoid X receptors (RXRs) that bind to specific retinoic acid response elements (RAREs) in the promoter regions of retinoid-target genes. Here, we characterized RAREs in the regulatory regions of the CMV and of the hepatitis B vi.us (HBV). The viral RAREs, i.e., CMV-RARE and HBV-RARE, are composed of two consensus RARE half-sites (A/GGGTCA) arranged as a direct repeat separated by 5-bp and 1-bp, respectively. The RAREs were activated by both RAR/RXR heterodimers and RXR homodimers in transient transfection experiments. We also found that COUP-TF$\alpha$ (chicken ovalbumin upstream promoter-transcription factor u) and COUP-TF$\beta$ repressed the retinoid response of the viral elements. Further we demonstrated that previously known retinoid antagonist, SRI 1330, repressed retinoid-induced transactivation of the CMV-RARE. These results implicate Vitamin A, it's nuclear receptors and COUP-TFs as important regulators of the CMV and HBV pathogenesis and the SRl1330 as potential negative modulator of such retinoid-dependent processes.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1996년도 추계학술대회 논문집
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• pp.891-895
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• 1996

Performance and efficiency of hybrid (hydraulic-mechanical) pick-up device of deep sea manganese nodules collector are very sensitive to altitude and altitude of pick-up head relative to undulating seafloor. For this reason, motion control of pick-up head relative to the changing deep sea topography and other disturbances is of particular importance in design of pick-up device. The concept of design axiom is applied to a pick-up device of hybrid type. Kinematic analysis conducted in absolute Cartesian coordinates gives position, velocity, and acceleration of the hydraulic cylinders which enable the pick-up head to keep the preset optimal distance from seafloor. Inverse dynamic analysis provides the driving forces of hydraulic cylinders and the reaction forces at each joint. Design sensitivity analysis is performed in order to investigate the effects of possible design variables on the change of the maximum strokes of hydraulic cylinders. The direct differentiation method is used to obtain the design sensitivity coefficients.

• 한국정밀공학회지
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• 제15권3호
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• pp.68-74
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• 1998

Performance and efficiency of deep seabed collector is a primary factor for feasibility of commercial deep ocean mining. The efficiency of manganese nodules collector depends on vehicle mobility relative to undulating seafloor and is attributed pickup head to keep altitude and elevation of it against seafloor. For this reason, motion control of pickup head relative to the changing deep-sea topography and other disturbances is of particular importance in design of pickup device. The concept of design axiom is applied to a pickup device of hybrid type in order to evaluate the concept design. Kinematic analysis conducted in absolute Cartesian coordinates gives position, velocity, and acceleration of the hydraulic cylinders which enable the pickup head to keep the preset optimal distance from seafloor. Inverse dynamic analysis provides the driving forces of hydraulic cylinders and the reaction forces at each joint. Design sensitivity analysis is performed in order to investigate the effects of possible design variables on the change of the maximum strokes of hydraulic cylinders. The direct differentiation method is used to obtain the design sensitivity coefficients.

• Journal of Periodontal and Implant Science
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• 제27권1호
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• pp.111-116
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• 1997

The oral cavity is easily accessible for direct exposure of a malignant disease. 1 percent of the oral malignant tumors are of metastatic origin and approximately 10 percent to 25 percent of the 1 percent fraction originate from the lungs. A case of metastatic lung carcinoma to the gingiva in a 88-year-old male is reported. He complained of pain and swelling between right maxillary 1st premolar and 2nd molar. Although surgical excision of the lesion has been done, the gingival lesion developed as a quickly growing mass and recurred 2 weeks after surgical excision. The gingival mass was histopathologically diagnosed as an undifferentiated carcinoma. Epithelial layer was continuous without ulceration and it seems that the cancer cells are originated from primary tumor. Infiltrated cancer cells were pleomorphic and dyskeratotic. The cells had 2 or more nuclei, not showing squamous or glandular differentiation. Immunohistochemical study revealed the cells originated from the epithelial cells. The prognosis is poor, because prognosis depends on surgical elimination of the primary tumor.

• Animal cells and systems
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• 제16권6호
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• pp.498-505
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• 2012

This study uses the mitochondrial DNA control region to identify the genetic diversity and population structure of the marbled soles (Pleuronectes yokohamae) that inhabit Jinhae Bay and Yokji Island in the nearby sea and the adjacent waters of Namhae, Hansan Island, and Jaran Bay. Direct sequencing of the PCR products revealed 379 bp sequences with 83 variable nucleotide sites, defining a total of 91 haplotypes. The haplotype diversity was high, ranging from $0.917{\pm}0.031$ to $0.983{\pm}0.008$, and nucleotide diversity ranged from $0.015{\pm}0.008$ to $0.024{\pm}0.012$. In addition, 48 haplotypes (52.7%) were unique. Pairwise $F_{ST}$ values were very low, with the maximum value occurring between PYH (Hansan Island) and PJI (Jinhae Bay) ($F_{ST}$ = 0.011). Therefore, no significant genetic differentiation was evident between any pair of sampling localities.

• 대한조선학회논문집
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• 제36권2호
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• pp.105-113
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• 1999

본 논문에서는 선체 단면의 St'Venant 비틂강성은 물론 굽힘-비틂 강성과 전단-비틂 강성 및 선체 개단면과 폐단면 연결부에서의 변위와 단면력의 연속조건을 고려한 보유추이론을 적용하여 수평 비틂 거동의 연성도가 크고, 비틂에 대해 유연한 선체 거더의 수평 비틂 연성 고유진동 감도해석방법을 제시하였다. 제시된 방법을 토대로 대형 컨테이너 운반선의 적화상태 변경시의 고유진동수를 감도해석 결과를 이용하여 추정한 결과는 통상적 재해석 결과와 비교하여 오차 1%이내의 매우 양호한 부합성을 나타내었다. 아울러, 수평거동과 비틂거동의 연성도가 큰 선박의 경우 선체 중량 중심이 형 깊이의 10%까지 변화할 경우에 고유진동수가 최대 8%까지 변화함을 확인하였다.

• 대한한의학원전학회지
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• 제28권2호
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• pp.61-66
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• 2015

Objectives : Through the analyze of formative factor of time sense and the vital movement temporal rhythm in Huang Di Nei Jing(黃帝內經). Methods : We find out the principle and methods of Chronomedicine and Health Preserving in Huang Di Nei Jing(黃帝內經), and this finding will direct the life nurturing for later generations. We find that time sense had been established in Huang Di Nei Jing(黃帝內經), which had yin-yang and five elements characteristic. Results : There have temporal rhythm of functions of zang-fu viscera, meridian and collateral, qi and blood. Based on the theory of correspondence between human body and natural environment, established principle of health maintenance about based on yin and yang, temporal rhythm of visceral qi. Conclusions : Established health maintenance methods based on time such as adjusting-spirit according to time, regular daily life, dietary regimen according to time, needling and moxibustion according to time. These methods will guide syndrome differentiation and therapeutic method and Medication which based on suitable time.

• 전산구조공학
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• 제11권1호
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• pp.161-170
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• 1998

길이가 긴 원통형 실린더를 구성하는 데에 사용될 조인트 부재에 대한 공차설계를 수행하였다. 즉, 원통형 실린더를 체결할 때 사용되는 조인트 부품 가운데 스터드 볼트를 최소 민감도해석에 의해 공차설계를 하였다. 조인트 부재의 공차설계를 위한 최소 민감도 해석에 의한 정식화는 목적함수가 폰 마이세스 응력의 공차에 대한 민감도이고, 여러 부등호 제약식 중에서 자중이 부등호 제안식에 포함된다. 조인트 부재의 경우 자중에 대한 타당한 부등호 제안식을 설정하기 위하여 우선 확정적인 경우에 대한 최적설계를 수행하여 그 범위값을 선정하였다. 원통형 부재의 구조 응답은 축대칭 유한요소로서 구조해석을 수행하여 제안식을 설정하였으며, 직접미분에 의해서 설계 민감도를 구하여 ,최적화 알고리즘과 결합하여 최적의 공차를 제시하였다.

• 대한기계학회논문집A
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• 제29권11호
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• pp.1527-1533
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• 2005

Three methods for design sensitivity analysis such as finite difference method(FDM), direct differentiation method(DDM) and adjoint variable method(AVM) are well known. FDM and DDM for design sensitivity analysis cost too much when the number of design variables is too large. An AVM is required to compute adjoint variables from the simultaneous linear system equation, the so-called adjoint equation. Because the adjoint equation is independent of the number of design variables, an AVM is efficient for when number of design variables is too large. In this study, AVM has been extended to the eigenproblem of damped systems whose eigenvlaues and eigenvectors are complex numbers. Moreover, this method is implemented into a commercial finite element analysis program by means of the semi-analytical method to show applicability of the developed method into practical structural problems. The proposed_method is compared with FDM and verified its accuracy for analytical and practical cases.