• 동의생리병리학회지
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• 제23권3호
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• pp.715-722
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• 2009

The aim of this study was to investigate the safety of short-term use of Korean herbal medicine (KHM; prescribed herbal medicine by doctors of traditional Korean medicine) on liver function. Three hundred eighty four outpatients who took KHM for various conditions were enrolled for multi-center, prospective observational study. Of them 237 patients completed questionnaire and were checked liver function (aspartic aminotransferase;AST, alanine aminotransferase;ALT, alkaline phosphatase;ALP, total bilirubin;t-Bil, direct bilirubin;d-Bil, gamma glutamyltranspeptidase;GGT, protein and albumin) before and after taking KHM (duration; 21.4${\pm}$10.0 days), and data were analysed statistically. Of the 213 patients showing normal liver function test (LFT) at baseline, 209 (98.1%) remained within the normal range at the second test while 3 (1.4%) revealed slight increase of LFT. Only one subject had raised LFT regarding level of liver injury without perceived symptoms. Twenty-four of 237 patients were abnormal at baseline, and 16 at the second testing. Of the patients taking KHM, only 4 changed from normal to abnormal while 12 from abnormal to normal in their LFT. There were no significant increase in LFT level between the first and second test, except in the t-Bil level, however, the change of t-Bil was small and within normal range. The current study showed that the use of KHM did not increase the frequency of abnormal LFTs, at least in the short term.

• 대한기계학회논문집B
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• 제38권9호
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• pp.781-787
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• 2014

PWMR(Pressurized wire mesh heating reactor)는 Intrinsic $CO_2$ 가스화 반응속도 해석에 필요한 활성화에너지와 빈도상수를 도줄하기 위해서 고안되었으며, 고압 및 고온(50atm, 1750K)조건 하에서 실험을 수행하였다. 본 연구에서는 고온조건에서의 실험을 위해 백금(Pt) 메쉬를 가열체로 사용하였고 직류전원공급기를 통해 백금메쉬로 전류를 인가하여 석탄 입자를 가열시킨다. 가열시 정확한 온도제어 및 반응시간 조절을 위해 직류전원공급기는 컴퓨터로 제어된다. 본 연구에서는, 인도네시아 아역청탄인 BERAU 를 사용하였으며 입자크기는 $90-150{\mu}m$, 압력과 온도조건은 각각 1-40atm 및 1373-1673K에서 실험을 진행하였다. 고압에서의 압력의 영향을 구분하기 위해 Internal/external effectiveness factor를 고려하였다. 최종적으로 BERAU 촤의 Intrinsic 가스화 반응속도론 을 $n^{th}$ order 반응식을 통해 도출하였으며 그 값은 203.8kJ/mol 의 값을 가졌다.

• 지역연구
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• 제32권2호
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• pp.3-13
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• 2016

본 연구에서는 부산에 있는 국가기록원에 보관중인 북한 지역 지적측량원도 299,688매를 디지털화하는 사업의 편익을 분단 상태에서도 발생하는 편익과 통일 이후에 발생하는 편익으로 나눠서 추정하였다. 분단상태에서도 발생하는 편익은 고유가치, 유산가치, 직 간접 이용가치, 군사적 전략적 가치, 통일 기반 조성으로 나눌 수 있다. 통일 이후의 편익은 토지 원소유권 확인을 위한 행정 비용 절감, 가계(家系) 확인, 지번부여와 지적관리 업무의 토대 조성, 도시계획적 활용 등이 있다. 실제 추정은 조건부가치추정법(CVM)의 단일경계모형을 사용하였다. 분단 상태에서 발생하는 편익에 대한 가구당 지불용의액은 향후 5년간 매년 7,925원이며, 총 편익은 5,868억 원으로 추정되었다. 통일 이후에 발생하는 편익에 대한 가구당 지불용의액은 8,023원이며, 토지 원소유권 반환여부에 따라 총 편익은 3,243~5,941억 원으로 추정되었다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2006년도 추계학술대회
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• pp.65-74
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• 2006

Modem drug discovery requires rapid pharmacokinetic evaluation of chemically diverse compounds for early candidate selection. This demands the development of analytical methods that offer high-throughput of samples. Naturally, liquid chromatography / tandem mass spectrometry (LC-MS/MS) is choice of the analytical method because of its superior sensitivity and selectivity. As a result of the short analysis time(typically 3-5min) by LC-MS/MS, sample preparation has become the rate- determining step in the whole analytical cycle. Consequently tremendous efforts are being made to speed up and automate this step. In a typical automated 96-well SPE(solid-phase extraction) procedure, plasma samples are transferred to the 96-well SPE plate, internal standard and aqueous buffer solutions are added and then vacuum is applied using the robotic liquid handling system. It takes only 20-90 min to process 96 samples by automated SPE and the analyst is physically occupied for only approximately 10 min. Recently, the ultra-high flow rate liquid chromatography (turbulent-flow chromatography)has sparked a huge interest for rapid and direct quantitation of drugs in plasma. There is no sample preparation except for sample aliquotting, internal standard addition and centrifugation. This type of analysis is achieved by using a small diameter column with a large particle size(30-5O ${\mu}$m) and a high flow rate, typically between 3-5 ml/min. Silica-based monolithic HPLC columns contain a novel chromatographic support in which the traditional particulate packing has been replaced with a single, continuous network (monolith) of pcrous silica. The main advantage of such a network is decreased backpressure due to macropores (2 ${\mu}$m) throughout the network. This allows high flow rates, and hence fast analyses that are unattainable with traditional particulate columns. The reduction of particle diameter in HPLC results in increased column efficiency. use of small particles (<2 urn), however, requires p.essu.es beyond the traditional 6,000 psi of conventional pumping devices. Instrumental development in recent years has resulted in pumping devices capable of handling the requirements of columns packed with small particles. The staggered parallel HPLC system consists of four fully independent binary HPLC pumps, a modified auto sampler, and a series of switching and selector valves all controlled by a single computer program. The system improves sample throughput without sacrificing chromatographic separation or data quality. Sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The process of Bioanalytical Method Validation is required by the FDA to assess and verify the performance of a chronlatographic method prior to its application in sample analysis. The validation should address the selectivity, linearity, accuracy, precision and stability of the method. This presentation will provide all overview of the work required to accomplish a full validation and show how a chromatographic method is suitable for toxirokinetic sample analysis. A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method developed to quantitate drug levels in dog plasma will be used as an example of tile process.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.96-97
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• 2012

In nitride and oxide film deposition, sputtered metals react with nitrogen or oxygen gas in a vacuum chamber to form metal nitride or oxide films on a substrate. The physical properties of sputtered films (metals, oxides, and nitrides) are strongly influenced by magnetron plasma density during the deposition process. Typical target power densities on the magnetron during the deposition process are ~ (5-30) W/cm2, which gives a relatively low plasma density. The main challenge in reactive sputtering is the ability to generate a stable, arc free discharge at high plasma densities. Arcs occur due to formation of an insulating layer on the target surface caused by the re-deposition effect. One current method of generating an arc free discharge is to use the commercially available Pinnacle Plus+ Pulsed DC plasma generator manufactured by Advanced Energy Inc. This plasma generator uses a positive voltage pulse between negative pulses to attract electrons and discharge the target surface, thus preventing arc formation. However, this method can only generate low density plasma and therefore cannot allow full control of film properties. Also, after long runs ~ (1-3) hours, depends on duty cycle the stability of the reactive process is reduced due to increased probability of arc formation. Between 1995 and 1999, a new way of magnetron sputtering called HIPIMS (highly ionized pulse impulse magnetron sputtering) was developed. The main idea of this approach is to apply short ${\sim}(50-100){\mu}s$ high power pulses with a target power densities during the pulse between ~ (1-3) kW/cm2. These high power pulses generate high-density magnetron plasma that can significantly improve and control film properties. From the beginning, HIPIMS method has been applied to reactive sputtering processes for deposition of conductive and nonconductive films. However, commercially available HIPIMS plasma generators have not been able to create a stable, arc-free discharge in most reactive magnetron sputtering processes. HIPIMS plasma generators have been successfully used in reactive sputtering of nitrides for hard coating applications and for Al2O3 films. But until now there has been no HIPIMS data presented on reactive sputtering in cluster tools for semiconductors and MEMs applications. In this presentation, a new method of generating an arc free discharge for reactive HIPIMS using the new Cyprium plasma generator from Zpulser LLC will be introduced. Data (or evidence) will be presented showing that arc formation in reactive HIPIMS can be controlled without applying a positive voltage pulse between high power pulses. Arc-free reactive HIPIMS processes for sputtering AlN, TiO2, TiN and Si3N4 on the Applied Materials ENDURA 200 mm cluster tool will be presented. A direct comparison of the properties of films sputtered with the Advanced Energy Pinnacle Plus + plasma generator and the Zpulser Cyprium plasma generator will be presented.

• 한국수정란이식학회지
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• 제9권2호
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• pp.145-152
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• 1994

The suitable electric stimulation is essential for activation and fusion of oocytes before or after nuclear transplantation The present study was undertaken to determine the optirnal condition for the parthenogenetic activation of in vitro rnatured(IVM) bovine oocytes by electric stimulation. Different direct current(DC) electric voltage of 1.0, 1.5 and 2.0 kV/cm and pulse duration of 30, 60 and 120 $\mu$sec were applied to the JVM nocytes in 0.3 M mannitol solution containing each 100 $\mu$M CaCl$_2$ and MgCl$_2$. IVM occytes at 24, 28 and 32 hours Post-maturation(hpm) were also electrically stimulated at 1.5 kV /cm, for 60 $\mu$ sec. The stimulated nocytes were then co-cultured in TCM-199 solution containing 10% fetal calf serum with bovine oviductal epithelial cells for 7~9 days in a 5% $CO_2$ incubator at 39$^{\circ}C$ ~ Their activation and in vitro development to morula and blastocyst were assessed under an inverted microscope. The higher activation rates 62.8 and 63.4% and in vitro de- velopment rates to morula and blastocyst 5.1 and 10.9% were shown in the oocytes stimulated at the voltage of 1.0 and 1.5 kV/cm than 2.0 kV/cm, respectively. No signifi- cantly(P<0.05) different activation rate was shown in JVM oocytes stimulated for 30, 60 and 120 $\mu$sec, but developmental rates to morula and blastocyst was significantly(P<0.05) higher in the oocytes stimulated for 30 $\mu$sec(6~3%) and 60 $\mu$sec(10~0%) than 120 $\mu$sec(0~ 0%). The aged oocytes at 28 and 30 hpm showed significantly(P<0.05) higher activation rates(72~7 and 79.7%) than the oocytes at 24 hpm(50~9%)~ Also, their developmental rates to morula and blastocyst were significantly(P<0.05) higher in the nocytes at 28(14.3%) and 32 hpm(15.9%) than 24 hpm(3.6%). From these results, it can be suggested that the optimal electric stimulation for IVM bovine occytes is a DC voltage between 1.0 and 1.5 kV/cm, pulse duration of 30 or 60 $\mu$sec, and the optimal age of IVM oocytes for electric activation is at 32 hpm.

• 수산해양기술연구
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• 제48권4호
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• pp.370-378
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• 2012

This study is to find out the distribution characteristic of Octopus minor in the Tando Bay on the southwest coast of Korea with conducting surveys from May of 2006 to April of 2007. Surveys were carried out at 20 stations on 2 and 3 tide time in the low speed of high tide at night and conducted during 30minutes (4 times fishing) per station. Catch per unit effort (CPUE) was estimated by total catch per 500hooks at each station. Monthly CPUE showed that they put highest in October, November, April and June at 10.4, 10.5, 9.4 and 7.7 individuals respectively. On the other hand, CPUE put lowest in January, the coldest month, and August, the hottest month, at 0.2 and 0.3 individuals respectively. As for geographical mantle length distribution, average mantle length ranged from 6.2cm to 7.3cm at stations near the tidal channels (St. 1, 4, 5, 6, 8, 9, 10, 13, 14, 15, 18, 19 and 20), and was smaller than 6.0cm at stations located inside of bay (St. 2, 3, 7, 11, 12 and 17). In terms of geographical distribution density, CPUE showed higher than 8.0 at St.4 in Changmaeri, St.8 in Taecheonri, St.13 near Seondo, St.18 in Naeri and St.14 in western part of Tando which are located near the main tidal channels. And distribution density showed low that CPUE was lower than 5.0 individuals at stations located inside of bay (St. 1, 3, 5, 6, 7, 11, 12, 16 and 20). Through the results, the difference of distribution density reflects that distribution of Octopus minor is affected by direction of current and inflow of pollutant from land in direct. Therefore, it is considered that bottom composition of tidal flat and distribution of live food are the crucial cause of identifying the distribution characteristics of Octopus minor. For this reason, it is judged to need additional research on it.

• 환경영향평가
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• 제23권4호
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• pp.271-284
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• 2014

본 연구는 금강 수계 일부 지역에서 한정적으로 서식하는 멸종위기 1급 어종인 미호종개 (Cobitis choii)의 서식 현황과 적합한 서식지 환경 조건을 파악하기 위하여 실시하였다. 미호종개 (Cobitis choii)는 미호천의 인근 수계 및 금강 일부 지역에 한정적으로 서식하는 한국 고유종으로 미호종개를 법적으로 보호하기 위해서 환경부 멸종위기종으로 지정하여, 현재 천연기념물 (제454호)로 지정되어있다. 조사는 백곡천, 유구천, 갑천에서 각각 실시하였으며, 미호종개 (Cobitis choii)의 개체수는 평균 2.6 개체가 채집되어 극히 희소하게 분포되어 있는 종으로써 종 보존이 아주 시급한 것으로 나타났다. 미호종개가 출현한 지역의 하상구조는 60% 이상이 가는 모래로 이루어져있고, 평균 1mm 크기의 모래입자에서 가장 높은 빈도로 출현하는 것으로 분석되었다. 또한, 최적 서식지 조건으로 최적 수심은 20 - 60 cm로 비교적 얕고, 최적 유속은 $0.4m^3/s$ 이하의 느린 흐름과 소가 반복적으로 나타나 최적 유량 범위가 $0-2m^3/s$ 인 구간에서 주로 서식하는 것으로 나타났다. 미소서식처는 하천정비 등의 인위적인 요인과 도심과 농경지로부터의 영양 염류 유입에 민감하게 반응하여 직접적인 영향을 주는 것으로 알려져 있다. 따라서, 멸종 위기종인 미호종개의 보존을 위해서 향후 대체서식지를 마련되어야 될 것으로 사료된다.

• 한국농림기상학회지
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• 제5권2호
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• pp.51-60
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• 2003

한국 내 농경지의 약 60%를 차지하고 있는 논은 아시아의 주요 농업 생태계의 하나이다. 논은 대기로부터 $CO_2$를 흡수함과 동시에 CH$_4$의 형태로 탄소를 대기 중으로 방출하는데, 관개 상태에 따라 흡수와 방출의 상대적 크기가 달라져서 온실 기체의 전구 수지에 중요한 변수로 작용할 수 있다. 온실 효과와 관련하여 논의 현재 및 미래의 역할을 보다 잘 이해하기 위하여, 주요 농업 생태계에서의 $CO_2$와 에너지 교환을 정량화 하는 것은 매우 중요하다. 이 연구의 목적은, 벼의 전체 생장 기간동안 한국의 전형적인 논에서의 에너지와 $CO_2$ 교환과정을 관측, 분석하고 이해하는 것이다. 2002년 4월부터 한국 타워 플럭스 관측 지역망(KoFlux)에속한 강화도 하리의 논에서 $CO_2$ 및 에너지 플럭스 관측을 지속적으로 해오고 있다. 다양한 보정 단계와 처리 과정을 거친 관측 자료를 통해, 논과 대기간의 $CO_2$ 교환량을 정량화하고, 이를 미기상학적, 생물리학적 관점에서 분석하였다. 날씨 변화와 더불어, 지표 피복 변화와 식물의 생장 단계에 따라 $CO_2$ 교환량이 민감하게 변화하였다. 국내 논에서의 전 생장기간에 걸친 에너지와 $CO_2$ 교환에 관한 최초의 직접 관측 보고인 본 연구는, 아시아 플럭스망(AsiaFlux) 내에서 중요한 참고 자료로서의 가치를 지니며, 지면 모형의 보정과 개선, 전구 생지화학적 순환에 대한 논의 역할, 그리고 생물-대기 상호 작용에 대한 근본적인 이해를 향상시키는 데 기여할 것이다.

• 한국콘텐츠학회논문지
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• 제12권7호
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• pp.261-272
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• 2012

본 연구는 작업기억 향상을 위한 직류두개자극(tDCS)이 뇌졸중 환자의 뇌파에 어떠한 변화를 가져오는지 알아보기 위해 시행되었다. MMSE와 숫자 외우기 검사(DST)를 통해 선별된 만성 뇌졸중 환자 20명을 전산화 인지 훈련(CCT)만 실시한 I군(10명)과 tDCS와 CCT를 병행한 II군(10명)으로 무작위 배치하여 주 3회, 1일 30분, 4주간 중재하였다. 뇌파 변화를 알아보기 위해 중재 전, 2주 후, 4주 후 시점에서 뇌전도로 단어, 사진, 암산과제 시 세가지 밴드(${\theta}$; 4~8 Hz, lower ${\alpha}$; 8~10.5 Hz, upper ${\alpha}$;10.5~13 Hz)로 절대 스펙트럼 파워를 산출하여, 기준뇌파에 대한 증감률(%)을 구하였다. 연구 결과, 첫째, 측정시점에 따라서, ${\theta}$ 파워는 단어기억과제와 사진기억과제에서 유의한 차이를 보였다. lower ${\alpha}$ 파워는 모든 기억과제에서 유의한 차이를 보였다. upper ${\alpha}$ 파워는 암산과제에서만 유의한 차이를 보였다. 둘째, 4주째에서 모든 과제의 lower ${\alpha}$ 파워에서만 두 군간에 유의한 차이를 보였다. 따라서 본 연구의 과제에 따른 세 가지 밴드의 차이를 통해 작업기억의 정도를 정량화 하여 비교가 가능하며, 뇌손상환자에게 tDCS의 병행이 인지재활에 보조적인 도움을 줄 수 있을 것으로 생각된다.