• 한국연초학회지
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• 제3권1호
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• pp.41-48
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• 1981

Dihaploid lines were derived from the anthers of the F, of single cross, "Va 115" x"SC 72" (Nicotiana tabacum L.). The path-analysis, correlation coefficient, and variation of characteristics among dihaploid families were evaluated. Dihaploid lines displayed much greater variation than their parents. The genetic correlation coefficient between yield and total alkaloids of dihaploids was significantly lower than that of conventional varieties and lines. In path-analysis which relates yield components to yield, number of leaves harvested and leaf width had a direct effect on yield. a direct effect on yield.

• 한국연초학회지
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• 제1권1호
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• pp.9-13
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• 1979

담배品種 ( Nicotiana tabacum L.) "SC 72"와 "Hicks" 를 文配하여 그$F_1$ 잡종식물의 葯으로부터 誘起한 반수체배가계통들의 특성을 檢定하였던 바, 交配親의 平均値에 비하여 生育特性은 현저히 저하하고, Total Alkaloids 는 증가하며, Reducing Sugar는 감소하는 傾向을 나타내었다.

• 한국연초학회지
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• 제21권1호
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• pp.82-88
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• 1999

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• 한국연초학회지
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• 제9권2호
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• pp.11-24
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• 1987

Lines were developed from the two crosses, BY4 X NC 82 and BY 4 X BY 104, by bulk, backcross and anther-derived dihaploid methods of breeding , and their variations in major agronomic, chemical characters, heritability, and response to selection were compared. 1. The anther-derived dihaploid lines showed a greater genetic variability and heritability in characters investigated than the lines developed by the other two methods of breeding . However, those were Inferior In agronomic characters and yielded 15.4% less leaf tobacco than the lines developed by the bulk method. 2. The lines developed by bulk method were higher In the frequency of lines resistant to bacterial wilt and in the yield, but Its lines appeared later flowering. inferior leaf Quality, and higher total nitrogen contents than those developed by the backcross method of breeding. 3. In the population of the lines developed by the backcross method, bacterial wilt resistant lines were less frequent, but the lines with a good leaf quality were more frequent.

• 한국연초학회지
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• 제15권2호
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• pp.137-144
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• 1993

The present study was conducted to compare the efficiency of individual plant selection for resistance to bacterial wilt with 30 dihaploid lines derived by anther culture and Nicotiana africana method in Fl and F2 generation from a cross between Bright Yellow 4 (BY4) and NC95. F2 dihaploid lines were selected from bacterial wilt disease resistant plants screened under the naturally infested filed conditions. The populations of FB - ADH and FB MDH derived from F2 individual plants with bacterial wilt resistance showed higher resistance to the disease than the populations of Fl - ADH and Fl - MDH, respectively, and no difference for the disease resistance appeared by the haploid deriving method within a generation.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1988년도 춘계 학술대회지
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• pp.94-95
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• 1988

• 한국연초학회지
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• 제4권2호
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• pp.31-36
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• 1982

The field evaluation were conducted on dihaploid progenies derived from anther culture of F1 plant of Drama x Sohyang (N. tabacum L.) The mean values of agronomic and chemical traits of dihaploids such as Nicotine, length/width, plant height, Leaf length, leaves per plant and Yield were greater than those of tar parents. The correlation coefficient of dihaploids were similar to those of conventional lines and varieties. In path analysis, in relating yield components to yield, leaves per plant and leaf width had a direct effect on Yield, but the other components such as plant height, leaf length and days to flowering influenced Yield indirect way.

• 식물조직배양학회지
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• 제26권3호
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• pp.189-195
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• 1999

녹색꽃양배추의 약배양에 영향을 미치는 제 조건을 규명하고 다양한 유전적인 특성을 가진 품종들을 대상으로 다수의 계통을 유기하여 신품종 육성 소재로 이용하고자 실험한 결과는 다음과 같다. 배지시험 결과 B5배지에 NAA 0.1 mg/L + 2.4-D 0.1mg/L + 당 10% 처리구에서 배상체 발생율이 가장 높았으며 액체배지가 고체배지보다 5배 정도 배발생에 효과적이었다. 약접종 직후 35$^{\circ}C$ 고온처리 일수에 따른 시험에서는 1일처리가 가장 효과적이었으며 시일이 경과할수록 배상체 발생율이 현저히 저하되었다 품종간 배상체 유기율은 Green valient 2.8%에서 Haisi의 21%까지 차이가 심하였으나 이배체율은 62~74%로 품종간 큰 차이를 나타내지 않았다. Rokguray와 Haisi두 품종 약유래 계통의 포장특성 검정결과 초장, 엽수, 측지수, 화뢰의 지름은 다양한 변이를 보였으며 두 품종에서 유기된 계통들 중 조숙이며 초장이 크고 화뢰의 지름이 큰 계통과 중만생이며 엽수가 많고 화뢰의 지름이 작은 계통을 각각 5계통, 7계통을 선발하여 F$_1$육성 소재로 이용하였다.

• 한국연초학회지
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• 제14권1호
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• pp.24-32
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• 1992

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single F t hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC 95, was used to develop the 30 dihaploid lines by anther culture(F1-ADH) and maternally-derived doubled haploid utilizing Nicotiana africana(F1-MDH), respectively. As compared with mid-parent, ADH lines showed increasing in number of leaves, delaying in days to flower and narrowing in leaf width. However, no significant differences in the other characters investigated were recognized. MDH lines also showed narrow leaf width, while no significant differences in the other characters were observed. The variations of the characters investigated were generally greater in ADH than MDH lines. MDH lines had higher plant height and shorter days to flower than ADH lines, while other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were plant height, leaf number, days to flower, and yield, while those showing relatively low value were leaf length, leaf width, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(F1-ADH, F1-MDH) and correlated responses associated with selection for yield were estimated. Plant height, leaf width, days to flower, percent reducing sugar and disease resistance would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• 한국작물학회지
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• 제27권1호
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• pp.87-93
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• 1982

담배 황색종 품종인 SC72와 Hicks를 교배하여 그 $F_1$잡종식물의 약으로부터 유기한 반수체식물을 배가시켜 얻은 30계통의 10개 양적형질에 대한 변이, 유전력, 유전상관 및 경로계수의 분석에 의하여 각형질들의 유전현상을 본바 그 결과를 요약하면 다음과 같다. 1. 교배친간 각 형질의 변이폭은 1.5~37.8%인데 비하여 반수체배가계통간에는 19.6%~100.1%로 현저히 크게 나타났고 평균치에 있어서는 교배친에 비하여 반수체배가계통들은 nicotine, 수확엽수 및 엽장폭비가 증가하는 경향이나 그외 특성들은 저하되는 경향이었다. 2. 유전력은 개화일수 및 수확엽수는 높게, reducing sugar, kg당 가격 및 수량은 낮게, 그외 형질들은 중간치를 나타내었다. 3. 유전상관의 값은 표현형상관보다 그 값이 다소 높은 경향이었고, 수량은 kg당 가격, 엽폭, 수확엽수 및 reducing sugar와는 정의 상관이었고, nicotine은 엽장폭비를 제외한 타형질과는 부의 상관을 나타내었다. 4. 수량과 각형질간의 경로계수를 분석한 결과 수확엽수와 엽폭이 수량에 미치는 직접효과가 컸으며, 간접효과는 개화일수와 초장이 크게 나타났다.