• Applied Microscopy
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• 제27권1호
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• pp.87-100
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• 1997

To investigate the effects of mercuric chloride $(HgCl_2)$ on the differentiation of the cerebral neuron of chick embryo 10 days, the ultrastructural changes in nerve cells injected with a various doses of mercuric chloride were observed with transmission electron microscope. The enzyme activity of the some dehydrogenases, cerebral proteins and adenosine triphosphate (ATP) were also analyzed. The results obtained are as follows; The ultrastructural changes in 1.0 mg-injected group, the nuclear membranes were irregular, outer of mitochondria membrances dispressioned, their cristae were destroyed. In 2.0 mg-injected group, the nuclear envelops were destroyed and divided, were not observed organelle except of few ribosome, the RER and mitochondria. The number of polypeptide bands were separated by SDS-PAGE in the normal group were 38 bands. According to the in creased dose of mercuric chloride, contends of the bands were increased in 4 bands, but were decreased in 1 band. The activities of dehydrogenases were declined by increasing the dose of mercuric chloride. Lactate dehydrogenase (LDH) activity fatted to 61% in 2.0 mg-injected group. Malate dehydrogenase (MDH) activity fatted to 90% in 1.0 mg-injected group, greatly to 76% in 2.0 mg-injected group. Succinate dehydrogenase (SDH) activity decreased to 79% in 1.0 mg-injected group and greatly to 62% in 2.0 mg-injected group. ATP content in 1.0 mg-injected group was almost near to the normal level, but it was increased greatly in 2.0 mg-injected group.

• Applied Microscopy
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• 제26권3호
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• pp.253-266
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• 1996

To investigate the effects of mercuric chloride ($HgCl_2$) on the differentiation of the cerebral neuron of chick embryo 9 days, the ultrastructural changes in nerve cells injected with a various doses of mercuric chloride were observed with transmission electron microscope. The enzyme activity of the some dehydrogenases, cerebral proteins and adenosine triphosphate (ATP) were also analyzed. The results obtained are as follows: The ultrastructural changes in 0.5 and 1.0mg-injected groups were undetectable, but in 2.0mg-injected group, the nuclear envelops were very irregular and mitochondria, were swelled and destroyed partly. The number of polypeptide bands separated by SDS-PAGE in the normal group were 37 bands. According to the in creased dose of mercuric chloride, contends of the bands were increased in 7 bands. The activities of dehydrogenases were declined by increasing the dose of mercuric chloride. Lactate dehydrogenase (LDH) activity failed to 78% in 1.0mg-injected group and greatly to 68% in 2.0 mg-injected group. Malate dehydrogenase (MDH) activity failed to 81% in 2.0 mg-injected group. On the other hand, succinate dehydrogenase (SDH) activity decreased to 80% in 1.0 mg-injected group and greatly to 63% in 2.0 mg-injected group. ATP content in 1.0 mg-injected group was increased slightly and in 2.0 mg-injected group was increased greatly.

• IMMUNE NETWORK
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• 제10권1호
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• pp.15-25
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• 2010

Background: Rat mast cells were regarded as a good model for mast cell function in immune response. Methods: Rat bone marrow mast cells (BMMC) were prepared both by recombinant rat IL-3 (rrIL-3) and by recombinant mouse stem cell factor (rmSCF), and investigated for both proliferation and differentiation in time course. Rat BMMC was induced by culture of rat bone marrow cells (BMCs) in the presence of both rrIL-3 (5 ng/ml) and rmSCF (5 ng/ml). Culture media were changed 2 times per week with the cell number condition of $5{\times}10^4/ml$ in 6 well plate. Proliferation was analyzed by cell number and cell counting kit-8 (CCK-8) and differentiation was by rat mast cell protease (RMCP) II and histamine. Results: Cell proliferation rates reached a maximum at 8 or 11 days of culture and decreased thereafter. However, both RMCP II production and histamine synthesis peaked after 11 days of culture. By real time RT-PCR, the level of histidine decarboxylase mRNA was more than 500 times higher on culture day 11 than on culture day 5. By transmission electron microscopy, the cells were heterogeneous in size and contained cytoplasmic granules. Using gated flow cytometry, we showed that cultured BMCs expressed high levels of $Fc{\varepsilon}RI$ and the mast cell antigen, ganglioside, on culture day 11. Conclusion: These results indicate that rat BMMCs were generated by culturing BMCs in the presence of rrII-3 and rmSCF and that the BMMCs have the characteristics of mucosal mast cells.

• 제어로봇시스템학회논문지
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• 제20권6호
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• pp.676-688
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• 2014

The IEEE 802.11e EDCA (Enhanced Distributed Channel Access) mechanism has been proposed to improve the QoS (Quality of Service) of various services in WLANs (Wireless Local Area Networks). By differentiating the channel access delay depending on ACs (Access Categories), this mechanism can provide the relative service differentiation among ACs. In this paper, we consider that WLAN is deployed in medical environments to transfer medical traffic and we reveal that the quality of the medical traffic (in particular, ECG signals) is significantly deteriorated even with the service differentiation by IEEE 802.11e EDCA. Also, we analyze the reason for performance degradation and show that IEEE 802.11e EDCA has difficulty in protecting the transmission opportunity of high-priority traffic against low-priority traffic. In order to assure medical-grade QoS, we firstly define the service priority of medical traffic based on their characteristics and requirements, and then we propose the enhanced channel access scheme, referred to as DIFF-CW. The proposed scheme differentiates CW (Contention Window) depending on the service priority and modifies the channel access procedure for low-priority traffic. The simulation results confirm that the DIFF-CW scheme not only assures the QoS of medical traffic but also improves the overall channel utilization.

• 디지털융복합연구
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• 제20권4호
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• pp.459-468
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• 2022

의료 현장에서는 매우 고해상도의 이미지를 사용하고 있으며, 이는 손실에 매우 민감한 정보이다. 이에 따라 높은 대역폭뿐만 아니라 고신뢰성 전송을 제공할 수 있는 광 인터넷의 활용이 요구되고 있다. 그러나 인터넷의 특성상 다양한 종류의 데이터가 동일한 대역폭을 활용하게 되고, 이를 효과적으로 차별화할 수 있는 수단이 요구되고 있다. 이를 위해 광 지연 라인 버퍼가 많이 활용되고 있다. 그러나, 이러한 버퍼는 제공 부하, 측정된 데이터 버스트 크기, 기본 지연 유닛 등과 같은 최적값을 이용해 구성된다. 광 버퍼는 한 번 설정되면 변경할 수 없다. 그러므로 데이터 버스트 크기를 동적으로 변경시키는 방법이 활용되고 있다. 그러나 동적으로 버스트의 길이를 변화시키는 것은 상당한 불안정성을 내포하고 있다. 이에 본 논문에서는 안정적인 동작을 보장할 수 있는 동작 조건을 분석하고자 한다. 본 논문의 기법을 활용해 높은 우선순위의 고해상도 의료 데이터를 손실 없이 안정적으로 전송할 수 있다.

• 전자공학회논문지CI
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• 제47권6호
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• pp.28-39
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• 2010

본 논문은 IEEE 802.11p에 기반한 CSMA의 미디어 접근 제어로서, 경쟁 차량단말기(OnBord Unit, OBU)의 증가에 따른 전송 지연(transmission delay)과 감소(data throughput decrease) 문제를 해결하고자 한다. 경쟁 기반의 전송매체에서는 OBU 증가에 따라 충돌 확률이 높아진다. 이러한 미디엄 계층의 성능 향상을 위해 교통량과 데이터 종류를 고려하여 접속 단말기의 개별 충돌 윈도(Contention Windows, CW)를 동적으로 조절하는 EDCA(Enhanced Distributed Channel Access)를 제안하였다. EDCA는 채널별 서비스 차별화를 위해 네 개의 클래스를 AC(Access Category)마다 고정된 최소 경쟁 윈도우(CWmin) 값과 최대 경쟁 윈도우(CWmax) 값을 적용한다. EDCA는 트래픽 특성별로 구분되어 AC 간의 차별화를 보장하지만, IEEE 802.11p 에 구성된 채널별 특성과 네트워크 상태를 보장하지 않기 때문에, 짧은 채널 서비스별 혼잡에 따른 충돌 발생을 능동적으로 대처할 수 없다. 이에 따른 해결방안으로 CWminAS(CWmin Adaptation Scheme)와 ACATICT(Adaptive Contention window Adjustment Technique based on Individual Class Traffic)가 능동적인 CW의 제어 기법으로 제시되었다. 선행연구는 개별 AC 값에 따라 발생하는 충돌 확률을 고려하지 않거나 단일 채널 기반의 개별 AC 값만 고려하고, IEEE 802.11p의 채널별 요구 사항과 이에 따른 충돌 확률을 반영하지 않았다. 본 연구는 이전 서비스 구간에서 OBU 경쟁에 따라 발생하는 충돌 횟수와 현재 무선망의 혼잡을 고려하여, 다음 채널의 CW를 능동적으로 제어하는 기법으로 ACCW(Adaptive Control of Contention windows in considering the WAVE situation)를 제안한다.

• 대한핵의학회지
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• 제31권1호
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• pp.90-101
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• 1997

FDG-PET의 반정량적인 당대사지표인 pSUV, aSUV, $TBR_{51}$과 $TBR_{area}$는 비관혈적으로 악성과 양성 연부조직 병변을 감별하는 데 모두 높은 정확도를 나타내었다. FDG-PET은 연부조직 종양의 평가에서 MRI와 상호보완적인 역할을 할 수 있을 것이다. 또한 FDG-PET은 악성 연부조직 종양환자들의 전이의 발견과 치료 후 추적관찰에도 도움이 될 것으로 생각되며 이에 관한 연구가 계속되어야 할 것이다.

• Applied Microscopy
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• 제29권3호
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• pp.291-301
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• 1999

초기발생중인 태령 9주부터 20주의 사람 태아의 허파 7예에서 과립허파꽈리세포의 분화과정과 미세구조의 특징을 밝히고자 투과전자현미경으로 관찰한 결과는 다음과 같다. 1. 발생 9주와 12주에서 기관지 나무의 상피의 형태로는 과립허파꽈리세포의 분화를 확인할 수 없었지만 발생이 진행되면서 15주 후부터 허파꽈리를 형성할 부위의 상피는 점차 거짓중층상피에서 단순입방상피로 분화하였다. 2. 발생 9주에서 처음으로 뭇층판소체가 관찰되어 과립허파꽈리세포의 분화를 확인할 수 있었다. 이외에도 발생 $9\sim15$주 동안에 이 세포에 특징적인 세포질포함체, 과립상포함체, 뭇소포포함체 그리고 진한포함체 등의 세포내 포함물을 관찰할 수 있었다. 3. 발생 19, 20주에서 뭇층판소체는 수와 크기가 급격하게 증가하였지만 다른 세포내 포함물은 감소되어 있었다. 요약하면 사람 태아의 허파에서 발생 초기단계에 뭇층판소체를 가진 허파꽈리세포(또는 전구세포)가 관찰되었으며, 이외에도 특징적인 다양한 세포내 포함물을 가진 세포들이 관찰되었다. 그러므로 과립허파꽈리세포는 발생 9주 또는 그 이전부터 분화한다고 할 수 있다.

• 동의신경정신과학회지
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• 제11권2호
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• pp.63-78
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• 2000

Headache is one of the most common medical complaints. It is not so easy to manage headache. especially if it is chronic although it seldom cause serious problem. There are many psychological factor known to induce, maintain an aggravate symptom in patients with chronic headache. The purpose of this study is to investigate clinical characteristics with ABR-2000 was carried out for 57 patients who had been suffered from headache for 6 months from march to August 2000.The results were as follows:1. According to the statics, on the whole woman's rate was higher than man's, and the mean duration of the headache was 8.06 years.2. Common associated symptoms were nausea. dyspepsia, dizziness, palpitation, fatigue, depression, etc.3. According to oriental medical differentiation of symptoms and signs, the rate of stagnation of the liver-qi's fire-transmission(肝變化火), deficiency of blood(血虛) and plegm syncope (痰厥), these three types were hghest.4. Result of analyzing ABR-2000 is that the rate of low response is higher than high response's on each item. But result of Graph A is that the rate of high response in higher than that of low response only in deficiency of Yin(陰虛) and the rate of high response in stagnation of the liver-qi's fire-transmission(肝變化火) and deficiency of blood (血虛) is comparatively high. Result of Graph R is that the rate of high response in wind-heat(風熱), deficiency of blood(血虛) and plegm syncope(痰厥) is comparatively higher than in others.

• Restorative Dentistry and Endodontics
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• 제22권2호
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• pp.519-535
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• 1997

The responses of human pulp fibroblastic cells to Ga-As Semi-Conductor-Dens-Bio Laser (Frequency: 5 Hz~10,000 Hz Model: SD-101A RCA, U.SA)) were examined in vitro using pulp fibroblastic cells obtained from the pulp tissue of human tooth. The mitogenic effect of soft laser was assessed by measuring the MTT assay. The morphologic effect for soft laser showed under the scanning and transmission electron microscopy. The results as follows; 1. The mitogenic response of the soft laser was not observed until 4th time of radiation, while the mitogenic response at 4th time increased mitogenic effect by as much as 1.7 fold compared to the control value. 2. The mitogenic response of the soft laser on pulp fibroblast differ from the mitogenic response on other fibroblasts. 3. In scanning electron microscopic study, The microvilli of cell surface increased gradually with width and length after laser radiation, it demonstrate that development of microvilli have close connection with differentiation of cells. 4. Under the transmission electron microscope, The laser-treated cells maintained their elongated shape and a high degree of cellular polarization. The large cell body containing a well developed Golgi complex, a large number of profiles of rough endoplasmic reticulum, and great numbers of mitochondria. 5. The laser-treated cells maintained the long straight bundles of closely apposed microfilaments or individual filaments forming a cross-linked network. These findings suggest that the laser may have important roles in promotion of pulp healing and consequently may be useful for clinical application in pulp regenerative procedures.