• Clinical and Experimental Reproductive Medicine
• /
• 제29권4호
• /
• pp.237-243
• /
• 2002

Objective : The aim of this study was to evaluate the influence of different media on blastulation, mean cell number, percentage of inner cell mass (ICM) of total cells and ICM : trophectoderm (TE) ratio in mice. Materials and methods: A total 552 two cell embryos were retrieved from ICR female mice (4 weeks old) at 48 hr after hCG injection (mated just after hCG injection) and cultured in MEM (n=276) or TCM (n=276) supplemented with 20% hFF. The grading of blastocysts from zona-intact (ZiB) to -escape (hatching and hatched, ZeB) was performed at 72 hours after culture. Total, TE and ICM cell numbers were analyzed by differential staining of blastocyst. Statistical analysis was performed using t-test with SigmaPlot-2001. P-values < 0.05 were accepted as statistically significant. Results: The blastulation rate in MEM ($64.9{\pm}4.95%$) was significantly higher (p=0.0031) than that in TCM ($57.2{\pm}5.22%$). No differences were found in the number of ZiB and ZeB between MEM ($31.9{\pm}2.62$, $33.0{\pm}4.58%$), and TCM ($27.2{\pm}4.28$, $30.1{\pm}4.58%$). A total 314 blastocysts (MEM=166; TCM=148) were stained differentially. Mean cell number of blastocysts was significantly higher (p=0.0002) in TCM ($73.1{\pm}3.3$) than in MEM ($61.7{\pm}2.5$). Differential staining was successfully performed in 155 blastocysts (MEM=77; TCM=78). The percentage of ICM was significantly higher in MEM than in TCM ($20.9{\pm}1.3$ vs. $17.1{\pm}1.2%$, p=0.0281). The ICM : TE ratio was higher in TCM than in MEM (1 : $4.85{\pm}0.68$ vs. 1 : $3.78{\pm}0.78$, NS). Conclusion: These results show that MEM increase the blastocyst formation and percentage of ICM of total cells comparing with TCM in mice.

• 만화애니메이션 연구
• /
• 통권27호
• /
• pp.221-247
• /
• 2012

일본인의 삶의 뿌리를 이루고 있는 전통문화에 대한 이해, 시대의 변화에 따른 전통 가치관의 변화 양상을 살펴보기 위해서는 유비쿼터스 사회의 미디어 속에 투영된 현대 일본인의 모습을 파악할 필요가 있다. 이를 위해 본 연구에서는 유비쿼터스 사회의 미디어에 나타난 일본인의 가치관 변화 양상과 사회문화적 배경이 되는 문화적 뿌리(전통문화)를 찾아보기 위하여, 현대 일본인이 자주 접하는 다양한 매체(휴대폰, 블로그, 캐릭터, 영화)에 나타난 전통의식과 전통문화에 대해 살펴보았다. 본 연구의 연구결과는 다음과 같다. 첫째, 일본의 젊은이들은 휴대폰을 타인의 프라이버시에 개입하고 싶지 않다는 옅은 친밀성을 강화하는 수단으로 사용하고 있다. 둘째, 일본인은 본심의 개방이라는 측면에서 익명성을 바탕으로 한 일기 형식의 블로그를 선호하는 편이다. 셋째, 일본의 여고생 및 직장여성이 가장 많이 사용하는 "귀엽다"라는 표현은 안심감, 위로를 받으려는 일본적 전통 문화에서 출발하였으며, 일본의 캐릭터의 배경이 되는 문화요소로서 불상, 부적, 요괴전설, 칠복신, 복고양이 등을 들 수 있다. 넷째, 일본의 영화에서 나타난 전통가치를 의리 야쿠자, 스모, 인간관계(남녀 가족 이웃)측면에서 살펴보았다. 현대 일본인이 자주 이용하는 매체를 대상으로 한 전통문화에 대한 연구 결과는 일본 콘텐츠 비즈니스 분야에 진출하고자 하는 기업이 성공적인 비즈니스 모델을 구현할 수 있는 전략 설정에 활용될 수 있으며, 일본 진출 과정에서 일본인의 요구에 맞는 콘텐츠 및 서비스를 발굴하는데 기초적인 자료로서 활용될 수 있기를 기대한다. 또한 일본인의 공동체 삶의 공간이 오프라인 중심에서 온라인으로 서서히 이동하는 사회문화적 변화양태를 고찰함으로써 현대 일본인의 삶과 문화를 이해하는데 기초적인 단서를 제공할 것으로 기대한다.

• Journal of Plant Biotechnology
• /
• 제6권1호
• /
• pp.15-19
• /
• 2004

The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

• Molecules and Cells
• /
• 제37권10호
• /
• pp.734-741
• /
• 2014

Histone modifications on major transcription factor target genes are one of the major regulatory mechanisms controlling adipogenesis. Plant homeodomain finger 2 (PHF2) is a Jumonji domain-containing protein and is known to demethylate the histone H3K9, a repressive gene marker. To better understand the function of PHF2 in adipocyte differentiation, we constructed stable PHF2 knock-down cells by using the mouse pre-adipocyte cell line 3T3-L1. When induced with adipogenic media, PHF2 knock-down cells showed reduced lipid accumulation compared to control cells. Differential expression using a cDNA microarray revealed significant reduction of metabolic pathway genes in the PHF2 knock-down cell line after differentiation. The reduced expression of major transcription factors and adipokines was confirmed with reverse transcription- quantitative polymerase chain reaction and Western blotting. We further performed co-immunoprecipitation analysis of PHF2 with four major adipogenic transcription factors, and we found that CCATT/enhancer binding protein (C/EBP)${\alpha}$ and C/EBP${\delta}$ physically interact with PHF2. In addition, PHF2 binding to target gene promoters was confirmed with a chromatin immunoprecipitation experiment. Finally, histone H3K9 methylation markers on the PHF2-binding sequences were increased in PHF2 knock-down cells after differentiation. Together, these results demonstrate that PHF2 histone demethylase controls adipogenic gene expression during differentiation.

• Journal of Microbiology and Biotechnology
• /
• 제28권1호
• /
• pp.22-31
• /
• 2018

Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at $37^{\circ}C$. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to $500{\mu}g/ml$ of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL ($100{\mu}g/ml$) could highly suppress the content of melanin and melanosome formation in B16-F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.

• The Korean Journal of Physiology and Pharmacology
• /
• 제21권2호
• /
• pp.161-168
• /
• 2017

Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-$1{\alpha}$ and K562) in vitro using $Transwell^{(R)}$ co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-$1{\alpha}$ and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.

• 대한의용생체공학회:의공학회지
• /
• 제28권2호
• /
• pp.310-315
• /
• 2007

The axial-flow type blood pump(XVAD) which has been developed in our group consists of mechanical parts (an impeller, a diffuser and a flow straightener) and electrical parts (a motor and a magnetic bearing). The magnetic bearing system fully levitates the impeller to remove mechanical coupling with other parts of the pump with constant gap, which needs non-contact type gap sensing. Conventional gap sensors are too large to be adopted to the implantable axial -flow type blood pump. Thus, in this paper, the compact eddy current type gap sensor system proper for the implantable axial-flow type blood pump was developed and its performance was evaluated in vitro. The developed eddy current type gap sensor system is a transformer type and has a differential probe. Sensor coil(probe) has small dimensions(6 mm diameter, 2 mm thickness) and its optimal inductance was determined as 0.068 mH for the measurement range of $0\sim3mm$. It could be manufactured with 130 turns of the 0.04 mm diameter copper coil. The characteristics of the developed eddy current type gap sensor system was evaluated by in vitro experiment. At experiment, it showed satis(actory performance to apply to the magnetic bearing system of the XVAD. It could measure the gap up to 3mm, but the linearity was decreased at the range of $1.8\sim3.0mm$. Moreover, it showed no difference in different media such as the water and the blood at the temperature range of $35\sim40^{\circ}C$.

• 방송공학회논문지
• /
• 제22권6호
• /
• pp.724-733
• /
• 2017

바둑 기보를 자동으로 기록하는 기존의 방법들은 대국 중 발생하는 방해물(손 혹은 물체)의 바둑판 가림 현상을 제대로 고려하지 않았다. 방해물에 의해 바둑판이 가려지는 경우 바둑돌의 착수 위치를 인식하지 못하거나, 바둑돌의 착수 순서가 실제와 다르게 저장되는 문제가 발생할 수 있다. 제안된 알고리즘은 방해물이 없는 온전한 바둑판 영상만을 배경 영상으로 내부에 저장하고 배경 영상과 현재 입력 영상을 비교하여 방해물을 인식한다. 그림자가 방해물로 오인식되는 현상을 제거하기 위해 단순한 차 영상이 아닌 미분영상을 기반으로 한 방해물 검출 방법이 제안되었다. 추가로 노이즈에 강인하게 방해물을 인식하기 위한 노이즈 제거 방법도 제안되었다. 방해물이 없는 때는 배경 영상을 지속적으로 갱신한다. 최종적으로 각 순간마다 저장된 배경 영상들을 비교하여 바둑돌의 착수 위치와 바둑돌의 종류를 인식한다. 실험 결과에 따르면 일반적인 조명환경에서 제안된 알고리즘은 95%이상의 인식률을 보여준다.

• 한국지하수토양환경학회:학술대회논문집
• /
• 한국지하수토양환경학회 2002년도 총회 및 춘계학술발표회
• /
• pp.122-126
• /
• 2002

Contaminant transport in porous media is characterized by solving an advection-dispersion equation(ADE). The ADE can cover equilibrium phenomena of interest, which include sorption, decay, and chemical reactions. Among these phenomena, sorption mechanism is described by several types of sorption isotherm. If we assume the sorption isotherm as linear, the solution of ADE can be easily procured. However, if we consider the sorption isotherm as non-linear isotherm like a Dual Reactive Domain Model (DRDM), the resulting differential equation becomes non-linear. In this case, the solution of ADE cannot be easily acquired by an analytic method. In this paper, we present the numerical analysis of ADE using a DRDM. The results reveal that even if sorption data may be fitted well using linear or non-linear isotherm, the characteristics of contaminant transport of the two cases are different from each other. To be concrete, the retardation of linear isotherm has stronger effect than that of the DRDM. As the non-linearity of sorption isotherm increases, the difference of retardation effects of the two cases becomes larger. For a pulse source, the maximum concentration of the linear model is higher than that of the DRDM, but the plume of the DRDM moves faster than that of the linear model. Behaviors of contaminant transport using the DRDM are consistent with common features of a linear model. For instance, biodegradation effect becomes larger as time goes by The faster the seepage velocity is, the faster the plume of contaminant moves. The plume of the contaminant is distributed evenly over overall domain in the event of high dispersion coefficient.

• Environmental Analysis Health and Toxicology
• /
• 제30권
• /
• pp.7.1-7.7
• /
• 2015

Objectives The widely promising applications of graphene nanomaterials raise considerable concerns regarding their environmental and human health risk assessment. The aim of the current study was to evaluate the toxicity profiling of graphene family nanano-materials (GFNs) in alternative in vitro and in vivo toxicity testing models. Methods The GFNs used in this study are graphene nanoplatelets ([GNPs]-pristine, carboxylate [COOH] and amide [$NH_2$]) and graphene oxides (single layer [SLGO] and few layers [FLGO]). The human bronchial epithelial cells (Beas2B cells) as in vitro system and the nematode Caenorhabditis elegans as in vivo system were used to profile the toxicity response of GFNs. Cytotoxicity assays, colony formation assay for cellular toxicity and reproduction potentiality in C. elegans were used as end points to evaluate the GFNs' toxicity. Results In general, GNPs exhibited higher toxicity than GOs in Beas2B cells, and among the GNPs the order of toxicity was pristine > $NH_2$ > COOH. Although the order of toxicity of the GNPs was maintained in C. elegans reproductive toxicity, but GOs were found to be more toxic in the worms than GNPs. In both systems, SLGO exhibited profoundly greater dose dependency than FLGO. The possible reason of their differential toxicity lay in their distinctive physicochemical characteristics and agglomeration behavior in the exposure media. Conclusions The present study revealed that the toxicity of GFNs is dependent on the graphene nanomaterial's physical forms, surface functionalizations, number of layers, dose, time of exposure and obviously, on the alternative model systems used for toxicity assessment.