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Accumulation characteristics and correlation analysis of five ginsenosides with different cultivation ages from different regions

  • Xiao, Dan;Yue, Hao;Xiu, Yang;Sun, Xiuli;Wang, YiBo;Liu, ShuYing
    • Journal of Ginseng Research
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    • v.39 no.4
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    • pp.338-344
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    • 2015
  • Background: Ginseng (the roots of Panax ginseng Meyer) is a well-known traditional Oriental medicine and is now widely used as a health food. It contains several types of ginsenosides, which are considered the major active medicinal components of ginseng. It has recently been reported that the qualitative and quantitative properties of ginsenosides found in ginseng may differ, depending on cultivation regions, ages, species, and so on. Therefore, it is necessary to study these variations with respect to cultivation ages and regions. Methods: In this study, 3-6-yr-old roots of P. ginseng were collected from three different cultivation regions. The contents of five ginsenosides (Rb1, Rd, Rc, Re, and Rgl) were measured by rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry. The Kruskal-Wallis Rank sum test and multiple t test were used for comparative analysis of the data to evaluate the dynamic changes in the accumulation of these ginsenosides affected by cultivation regions and ages. Results: The content and composition of ginsenosides varied significantly among specimens collected from different cultivation regions and having different cultivation ages. For all samples, the content of Rg1 and Re ginsenosides increases with age and this rate of increase is different for each sample. The contents of Rb1, Rc, and Rd varied with cultivation ages in samples from different cultivation regions; especially, Rb1 from a 6-yr-old root showed approximately twofold variation among the samples from three cultivation regions. Furthermore, the content of Rb1 highly correlated with that of Rd (r = 0.89 across all locations and ages). Conclusion: In our study, only the contents of ginsenosides Rg1 and Re were affected by the root age. Ginsenosides Rb1, Rc, and Rd varied widely with ages in samples from different cultivation regions.

A Study on the Regional Characteristics of Korean Chotkal -The kinds and materials of chotkal- (우리나라 젓갈의 지역성 연구 (1) -젓갈의 종류와 주재료-)

  • Suh, Hye-Kyung;Yoon, Seo-Seok
    • Journal of the Korean Society of Food Culture
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    • v.2 no.1
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    • pp.45-54
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    • 1987
  • This paper is a part of the regional characteristics of Korean chotkal. The kinds and materials of Korean chotkals are studied by interviewing local people living in 142 different regions which are bigger than 'myon' in size. Regions are classified according to the structural style of commoner's house. Regional characteristics of chotkal are analyzed and interpreted with natural environmental factor. The rest of my further interested subject will be followed in later issues ; the ways of preservation, period of fermentation according to the regional characteristics and uses of Korean chotkal. The results of this study can be summerized as follows, 1. There are 145 different kinds. 2. Raw materials of 145 kinds of chotkal are devided roughly into five groups ; (1) fishes in 87 kinds (2) Pelecypoda and Gastropoda in 14 kinds (3) Cephalopoda in 10 Kinds (4) Crustacea in 32 kinds and (5) Holothuroidea and Echinoidea in 2 kinds. They can also be classified according to the parts of individual material ; (1) body or flesh in 118 kinds (2) internal organs in 15 kinds and (3) eggs in 12 kinds. Regions are devided into four provinces ; 'Kwanso', 'Chungbu', 'Nambu', and 'Kwanbuk' in which the number of different kinds of chotkal are 32, 41, 99 and 34, respectively. Raw materials vary because of the distribution of marine life in different regions. Fishes and Cephalopoda are used in all four regions, Pelecypoda and Gastropoda in 'Kwanso', 'Chungbu' and western part of 'Nambu' regions. Different species of Crustacea used in each different regions ; sea crab and sea shrimp in the western coastal area, fresh water crab in the field area, fresh water shrimp in 'Chollado', mantis crab at 'Shihung-gun' in 'Kyonggi-do', Holothuroidea and Echinoidea are used in 'Nambu' regions. Chotkal are not used at all in six local districts which are located in inland areas where the transportation is inconvenient.

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Nonparametric analysis of income distributions among different regions based on energy distance with applications to China Health and Nutrition Survey data

  • Ma, Zhihua;Xue, Yishu;Hu, Guanyu
    • Communications for Statistical Applications and Methods
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    • v.26 no.1
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    • pp.57-67
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    • 2019
  • Income distribution is a major concern in economic theory. In regional economics, it is often of interest to compare income distributions in different regions. Traditional methods often compare the income inequality of different regions by assuming parametric forms of the income distributions, or using summary statistics like the Gini coefficient. In this paper, we propose a nonparametric procedure to test for heterogeneity in income distributions among different regions, and a K-means clustering procedure for clustering income distributions based on energy distance. In simulation studies, it is shown that the energy distance based method has competitive results with other common methods in hypothesis testing, and the energy distance based clustering method performs well in the clustering problem. The proposed approaches are applied in analyzing data from China Health and Nutrition Survey 2011. The results indicate that there are significant differences among income distributions of the 12 provinces in the dataset. After applying a 4-means clustering algorithm, we obtained the clustering results of the income distributions in the 12 provinces.

Different Responses in Brain Regions upon Heat Shock in Adult Zebrafish (Danio rerio)

  • Hwang, Chang-Nam;Lee, Dong-Ho;Lee, Sang-Ho
    • Development and Reproduction
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    • v.13 no.3
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    • pp.199-205
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    • 2009
  • HSP70 has widely been induced in in vivo hyperthermia conditions in various organisms to study gene regulation and recently neuroprotectve roles of the induced gene expression under varying conditions. We investigated different responses among various tissues in zebrafish under heat shock to evaluate whether spatial and temporal expression pattern of zebrafish (z) hsp70 in transcriptional and translational level under heat shock stress in different brain regions. Heat shock groups were given for 1 h at $37^{\circ}C$ after recovery by transferring the treated animals back to $28^{\circ}C$ for 1, 2 and 24 h for recovery, respectively. Control (CTRL) group was kept at $28^{\circ}C$. At the end of treatments, five animals were collected and used for isolation of total RNAs and peptides from the corresponding tissues. Expression of zhsp70 mRNA showed different patterns in recovery periods in the tissues including the brain, eye, intestines, muscles, heart and testis by RT-PCR. Unlike the RT-PCR analysis, Northern blot analysis demonstrated nearly 30-fold increase in zhsp70 at 1 h heat shock, suggesting that RT-PCR may not be appropriate in unmasking regulation of the time-dependent zhsp70 expression. In the experiment involving different brain regions, the cerebellum showed gradual activation at 1 h to R1h and decreases in R2h and R24h, while the medulla oblongata and optic tectum showed gradual increase at R1h and decrease at R24h, indicating that different brain tissues respond specifically to heat shock in inducing zhsp70 and recovering from the heat shock status. Western blot analysis also demonstrated that the intracellular levels of zHSP70 in three different brain regions including the cerebellum, medulla oblongata and optic tectum are differently induced and recovered to normal state. These results clearly demonstrate that different regions of the body and the brain tissues are responding differently to heat shock in the aspects of its level of expression and speed of recovery.

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Effects of Different Growing Regions on Quality Characteristics, Bioactive Compound Contents, and Antioxidant Activity of Aronia (Aronia melanocarpa) in Korea

  • Hwang, Eun-Sun;Thi, Nhuan Do
    • Preventive Nutrition and Food Science
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    • v.21 no.3
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    • pp.255-262
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    • 2016
  • The objective of this study was to determine the effects of different growing regions on quality characteristics, total bioactive compound contents, and in vitro antioxidant activity in aronia. Aronia grown in 3 different regions (Sangjoo, Ulju, and Youngcheon) in Korea was obtained and used fresh or as a freeze-dried powder. No statistically significant differences were observed for moisture, ash, crude lipid, and crude protein contents in aronia sampled from the 3 different regions. Aronia grown in Sangjoo had the highest total acid content and the lowest sugar content and pH value. Conversely, aronia grown in Youngcheon possessed the lowest total acid content and the highest sugar content and pH value. Aronia grown in Sangjoo possessed relatively high levels of polyphenols, flavonoids, and anthocyanins, as well as high antioxidant activity in comparison with aronia produced in other regions. Aronia grown in Youngcheon scored the highest for taste and overall acceptability in sensory evaluations, which may be related to the high sugar content and pH, and the low total acidity of the fruits. It is possible that higher sugar contents and pH, and lower total acidity in the aronia grown in Youngcheon result in more preferable sensory characteristics. However, they also contain relatively low levels of total polyphenols, flavonoids, and anthocyanins, and have low antioxidant activity as measured by 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assays.

Study of Regional Differences Between Attitudes Toward Service and Behavior of Chinese Passengers Using Incheon International Airport (인천국제공항 이용 중국여객의 권역별 서비스 인식 및 행동특성에 관한 연구)

  • Lee, Myeong-U;Choe, Yeon-Cheol;Lee, Su-Mi
    • 한국항공운항학회:학술대회논문집
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    • 2015.11a
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    • pp.231-236
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    • 2015
  • Ever since the permission of overseas travel by the Chinese government in 1983, the Chinese outbound travel market has been growing at an expanding rate. The Republic of Korea has been able to reap great benefits from this growth, and this has been noticeable especially in the aviation market. Chinese passengers count for 23.9% of the total passengers at Incheon International Airport (IIA), and it is no doubt Chinese passengers will remain important customers for IIA. Given the fact that China has such a huge territory and possesses a population of more than 1.3 billion, there rises the need to divide China into smaller regions. By doing so, the different thoughts Chinese passengers have, and the actions they show in terms of travel and airport service consumption according to differing regions can be figured out. With knowledge of the differences, IIA can provide different services to fit Chinese passengers from differing regions, hence increasing the total consumer value and satisfaction rate of IIA. This study has divided China into 5 different regions according to official regional recognition of China, and difference of economical, social factors, etc... With the results obtained from this study, different strategies can be implemented for the 5 regions.

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On the use of alternative water use efficiency parameters in dryland ecosystems: a review

  • Kang, Wenping;Kang, Sinkyu
    • Journal of Ecology and Environment
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    • v.43 no.2
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    • pp.246-253
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    • 2019
  • Background: Water use efficiency (WUE) is an indicator of the trade-off between carbon uptake and water loss to the atmosphere at the plant or ecosystem level. Understanding temporal dynamics and the response of WUE to climatic variability is an essential part of land degradation assessments in water-limited dryland regions. Alternative definitions of and/or alternative methodologies used to measure WUE, however, have hampered intercomparisons among previous studies of different biomes and regions. The present study aims to clarify semantic differences among WUE parameters applied in previous studies and summarize these parameters in terms of their definition and methodology. Additionally, the consistency of the responses of alternative WUE parameters to interannual changes in moisture levels in Northeast Asia dryland regions (NADRs) was tested. Results: The literature review identified more than five different WUE parameters defined at leaf and ecosystem levels and indicates that major conclusions regarding the WUE response to climatic variability were partly inconsistent depending on the parameters used. Our demonstration of WUE in NADR again confirmed regional inconsistencies and further showed that inconsistencies were more distinct in hyper- and semi-arid climates than in arid climates, which might reflect the different relative roles of physical and biological processes in the coupled carbon-water process. Conclusions: The responses of alternative WUE parameters to drying and wetting may be different in different regions, and regionally different response seems to be related to aridity, which determines vegetation coverage.

Genomic Distribution of Simple Sequence Repeats in Brassica rapa

  • Hong, Chang Pyo;Piao, Zhong Yun;Kang, Tae Wook;Batley, Jacqueline;Yang, Tae-Jin;Hur, Yoon-Kang;Bhak, Jong;Park, Beom-Seok;Edwards, David;Lim, Yong Pyo
    • Molecules and Cells
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    • v.23 no.3
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    • pp.349-356
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    • 2007
  • Simple Sequence Repeats (SSRs) represent short tandem duplications found within all eukaryotic organisms. To examine the distribution of SSRs in the genome of Brassica rapa ssp. pekinensis, SSRs from different genomic regions representing 17.7 Mb of genomic sequence were surveyed. SSRs appear more abundant in non-coding regions (86.6%) than in coding regions (13.4%). Comparison of SSR densities in different genomic regions demonstrated that SSR density was greatest within the 5'-flanking regions of the predicted genes. The proportion of different repeat motifs varied between genomic regions, with trinucleotide SSRs more prevalent in predicted coding regions, reflecting the codon structure in these regions. SSRs were also preferentially associated with gene-rich regions, with peri-centromeric heterochromatin SSRs mostly associated with retrotransposons. These results indicate that the distribution of SSRs in the genome is non-random. Comparison of SSR abundance between B. rapa and the closely related species Arabidopsis thaliana suggests a greater abundance of SSRs in B. rapa, which may be due to the proposed genome triplication. Our results provide a comprehensive view of SSR genomic distribution and evolution in Brassica for comparison with the sequenced genomes of A. thaliana and Oryza sativa.

A Comparison of Sources of Sodium and Potassium Intake by Gender, Age and Regions in Koreans: Korea National Health and Nutrition Examination Survey (KNHANES) 2010-2012 (한국인의 성별, 연령별, 지역별 나트륨과 칼륨 섭취 현황 및 기여음식 : 2010-2012년 국민건강영양조사 분석)

  • Park, Yang-hee;Chung, Sang-Jin
    • Korean Journal of Community Nutrition
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    • v.21 no.6
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    • pp.558-573
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    • 2016
  • Objectives: The purpose of this study was to evaluate the main sources of dietary sodium and potassium intake in Koreans by gender, age and regions. Methods: We used the data from 2010-2012 KNHANES. A total of 20,387 subjects aged 8 years and older were included. Intakes were compared by gender, age (8-18, 19-49 and >50 years) and geographical regions in Korea. Dishes were classified into 28 dish groups based on cooking methods. Statistical analysis was performed by using the SAS 9.3 and SUDAAN 11.0.1 software. Results: The mean sodium intake of Koreans was $4866.5{\pm}35.9mg/day$, which was 2.4 times higher than the adequate intake (AI) of sodium for Koreans. We found that daily sodium intakes were significantly different by age, gender and regions. Men and aged over 50 years had significantly higher sodium intake than women and other age groups. The mean potassium intake in Koreans was $3002.2{\pm}19.4mg/day$ and daily potassium intakes were significantly different by age, gender and regions. Women and age 50 years and over had significantly higher potassium intakes than men and other age groups. The average Na/K ratio was $2.89{\pm}0.01$ and was highest in men and in the age group of 19-49 years. The major sources of dietary sodium were soup and stew, followed by Kimchi, noodles and dumpling, pickled vegetables and seasonings, which represented 63.1 % of total sodium intakes. Soup and stew or Kimchi were the primary sources of dietary sodium intake. The major sources of dietary potassium were cooked rice, followed by soup and stew, Kimchi, fruits and beverages. Conclusions: Sodium and potassium intakes and the major sources of those were significantly different by gender, age groups and regions. Therefore, different approaches based on gender, age and regions are needed to decrease sodium intake and increase potassium intake.

Localizing Growth Model of Chamaecyparis obtusa Stands Using Dummy Variables in a Single Equation

  • Lee, Sang-Hyun;Kim, Hyun
    • Journal of Korean Society of Forest Science
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    • v.94 no.2 s.159
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    • pp.121-126
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    • 2005
  • This study was carried out to construct a single diameter and a single height model that could localize Chamaecyparis obtusa stand grown in 3 Southern regions of Korea. Dummy variables, which convert qualitative information such as geographical regions into quantitative information by means of a coding scheme (0 or 1), were used to localize growth models. In results, modified form of Gompertz equation, $Y_2={\exp}({\ln}(Y_1){\exp}(-{\beta}(T_2-T_1)+{\gamma}({T_2}^2-{T_1}^2))+({\alpha}+{\alpha}_1Al+{\beta}_1k_1+{\beta}_2k_2)(1-{\exp}(-{\beta}(T_2-T_1)+{\gamma}({T_2}^2-{T_1}^2))$, for diameter and height was successfully disaggregated to provide different projection equation for each of the 3 regions individually. The use of dummy variables on a single equation, therefore, provides potential capabilities for testing the justification of having different models for different sub-populations, where a number of site variables such as altitude, annual rainfall and soil type can be considered as possible variables to explain growth variation across regions.