• Asian-Australasian Journal of Animal Sciences
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• 제28권4호
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• pp.551-558
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• 2015

This study was carried out to evaluate the dietary threonine requirement by measuring the plasma free threonine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A total of 70 fish (average initial weight $506{\pm}8.2g$) were randomly distributed into each of the 14 net cages (5 fish/cage). After 48 hours (h) of feed deprivation, each group was intubated at 1% body weight with one of the seven L-amino acid based diets containing graded levels of threonine (0.42%, 0.72%, 0.92%, 1.12%, 1.32%, 1.52%, or 1.82% of diet, dry matter basis). Blood samples were taken at 0, 5, and 24 h after intubation. Post-prandial plasma free threonine concentrations (PPthr) of fish 5 h after intubation with diets containing 1.32% or more threonine were significantly higher than those of fish intubated with diets containing 1.12% or less threonine (p<0.05). Post-absorptive free threonine concentrations (PAthr) after 24 h of intubation of the fish with diets containing 0.92% or more threonine were significantly higher than those of fish intubated with diets containing 0.72% or less threonine. Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) were not significantly different among fish intubated with diets containing 1.12% or less threonine, except the PPA of fish intubated with diet containing 0.42% threonine. Broken-line model analyses of PPthr, PAthr, and PPA indicated that the dietary threonine requirement of rainbow trout should be between 0.95% (2.71) and 1.07% (3.06) of diet (% of dietary protein on a dry matter basis).

• Fisheries and Aquatic Sciences
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• 제27권3호
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• pp.171-179
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• 2024

A study was conducted to evaluate dietary valine (Val) requirement for Pacific white shrimp (Penaeus vannamei). Five isonitrogenous (353 g/kg) and isocaloric (4.08 kcal/g) semi-purified diets containing graded levels of Val (2.7, 5.1, 8.7, 12.1 or 16.0 g/kg) were formulated. Quadruplicate groups of 12 shrimp (average body weight: 0.46 ± 0.00 g) were fed one of the experimental diets (2%-5% of total body weight) for 8 weeks. Maximum weight gain was observed in 8.7 g/kg Val group. However, the growth performance was reduced when Val concentration in diets were higher than 12.1 g/kg. Feed conversion ratio was significantly increased with 2.7 and 16.0 g/kg Val inclusion. Shrimp fed the diets containing 2.7 g/kg Val showed significantly lower protein efficiency ratio, whole-body crude protein and Val concentrations. Dietary inclusion of Val significantly improved the relative expression of insulin-like growth factor binding protein and immune-related genes (prophenoloxidase, lysozyme and crustin) in the hepatopancreas and 8.7 g/kg Val group showed highest expression among all the groups. The dietary requirement of Val for maximum growth of juvenile P. vannamei, estimated using polynomial regression analysis on growth, was 9.54 g/kg of Val (27.2 g/kg based on protein level) and maximum growth occurred at 9.27 g/kg of Val (26.2 g/kg based on protein level) based on broken-line regression analysis.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.19-19
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• 2003

This presentation reviewed the dietary vitamin C requirements in main marine finfish culture in Korea. In experiment I, an II-week feeding trial was conducted to study the effects of the different levels of dietary vitamin C on growth, tissue ascorbic acid concentrations and histopathological changes in parrot fish. Casein and gelatin based diets were formulated to contain 0, 60, 120, 240, 480 and 2000mg L-ascorbic acid (AA)kg diets on Ah equivalent basis in the form of L-ascorbyl-2-monophosphate (AMP), 60 and 240 mg AA/kg diet as L-ascorbic acid. Broken line analysis of weight gain indicated that the dietary vitamin C requirement of parrot fish is l18$\pm$12 mg AA/kg diet in the form of L-ascorbyl-2-monophosphate for maximum growth. In experiment II, a 12-week experiment was conducted to compare L-ascorbyl-2-monophosphate-Ca (AMP-Ca) with L-ascorbyl-2-monophosphate-Na/Ca (AMP-Na/Ca) for supplying the dietary vitamin C for juvenile Korean rockfish Sebastes schlegeli. Fish were fed one of 11 semi-purified diets containing equivalent of 0, 50, 100, 200, 400, and 800 mg ascorbic acid (AA)kg diet in the form of AMP-Ca or AMP-Na/Ca for 12 weeks. Broken line analysis of weight gain indicated that the dietary vitamin C requirement of Korean rockfish is 100 mg AA/kg diet in the form of AMP-Na/Ca, and 117 nag AA/kg diet in the form of AMP-Ca. In experiment III, a 12-week experiment was conducted to study the effects of different dietary levels of vitamin C, L-ascorbyl-2-polyphosphate (ASPP), on growth and tissue vitamin C concentrations in juvenile olive flounder. Fish were fed one of six semi-purified diets containing an equivalent of 0, 25, 50, 75, 150, or 1500 mg ascorbic acid (AA) kg 1 diet in the form of ASPP for 12 weeks. Based on broken line analyses for WG and PER, the optimum dietary levels of vitamin C were 91 and 93 mg AA/kg diet, respectively.

• Fisheries and Aquatic Sciences
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• 제19권1호
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• pp.4.1-4.7
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• 2016

This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was formulated to one of the seven L-amino acid based diets containing graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 % dry diet). A total of 35 fish averaging $512{\pm}6.8g$ ($mean{\pm}SD$) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group of fish was fed one of the experimental diets by intubation at 1 % body weight. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free lysine concentrations (PPlys, 5 h after intubation) of fish fed diets containing ${\geq}2.32%$ lysine were higher than those of fish fed diets containing ${\leq}1.92%$ lysine. Post-absorptive free lysine concentrations (PAlys, 24 h after intubation) of fish fed diets containing 2.32 and 3.52 % lysine were higher than those of fish fed diets containing ${\leq}1.52%$ lysine. The brokenline regression analysis on the basis of PPlys and PAlys indicated that the lysine requirement of rainbow trout could be 2.34 and 2.20 % in diet. Therefore, these results strongly suggested that the dietary lysine requirement based on the broken-line model analyses of PPlys and PAlys could be greater than 2.20 % but less than 2.34 % (corresponding to be $6.01%{\leq},but{\leq}6.39%$ in dietary protein basis, respectively) in rainbow trout. Also, these results shown that the quantitative estimation of lysine requirement by using PPlys and PAlys could be an acceptable method in fish.

• Journal of Nutrition and Health
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• 제27권8호
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• pp.844-855
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• 1994

The purpose of this study is to investigate the nutritional status and dietary intake of gastrectomized cancer patients. For this study, from 1993. 1 to 1993. 8, 50 postoperative gastric cancer patients were selected to examine anthropometric and laboratory data(Body Weight, Body Fat, serum Albumin, Total Lympocyte count), and dietary intake related symptoms. The results were 1) All anthropometric and laboratory data were significantly deteriorated by gastrectomy(s-Albumin, TLC. Body Fat : p<0.001). Weight loss of gastrectomized patients was 8.23$\pm$3.72% from admission to discharge. 2) In many gastrectomized cancer patients, preoperative dietary intake was decreased by abdominal discomfort, indigestion, early satiety, and anorexia. 3) Postoperative energy intake was 602$\pm$158㎉, and it is correspond to 31.18$\pm$.90% of daily energy requirement(1918$\pm$236㎉). The cause of poor oral intake is mostly fear, abdominal distension and fullness, and early satiety. In consideration of the fact that an inadequate energy intake was the main cause of the decreasing nutritional status, a careful nutritional care and dietary education is necessry after gastrectomy.

• Fisheries and Aquatic Sciences
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• 제24권3호
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• pp.129-137
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• 2021

Dietary protein requirements of abalone (Haliotis discus) depending on abalone size were determined and compared. One thousand and fifty small abalone (initial weight of 2.7 g) and five hundred forty large one (initial weight of 16.0 g) were distributed into 15 and 18 containers in Trial 1 and 2, respectively. Five and six experimental diets containing crude protein level from 20% to 40% and 20% to 45% with 5% increment of protein level for the small and large abalone were prepared and referred to as the CP20, CP25, CP30, CP35, CP40, and CP45 diets, respectively. The experimental diets were fed to abalone for 16 weeks in Trials 1 and 2. Specific growth rate (SGR) of the small abalone fed the CP20 diet was lower compared to that of abalone fed all other diets in Trial 1. Growth performance (weight gain and SGR) of the large abalone fed the CP30, CP35, and CP40 diets were greater than that of abalone fed the CP20, CP25, and CP45 diets in Trial 2. Dietary protein requirements were estimated to be 33.0% and 33.5% for the small and large abalone based on the 2^nd order polynomial analysis, respectively. Dietary protein requirements for the small abalone grown from 2.7 g to 7.4 g and the large one grown from 16 g to 21 g were estimated to be 33.0% and 33.5%, respectively. Size differences in abalone did not affect dietary protein requirement under this experimental conditions.

• 한국가금학회지
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• 제14권1호
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• pp.39-53
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• 1987

본시험은 산란계사료의 에너지 및 단백질수준이 산란성적, 생존율, 란질, 영양소이용율 및 복강지방 축적율 등에 미치는 효과를 구명하기 위하여 대사에너지 3수준(2,500, 2,700, 2,900 kcal/kg)과 단백질 3수준(13, 15, 17%)을 조합한 9개처리에 720수씩 2회에 걸쳐 1,440수를 공시하였으며, 1차시험은 1982년 5월 5일부터 1983년 5월 3일까지, 2차시험은 1983년 12월 28일부터 1984년 12월 26일까지 각각 52주간에 걸쳐 시험을 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 산란율은 대사에너지수준이 증가할수록 점차 감소하였고 단백질 수준이 증가할수록 점차 증가하였지만 (P〈0.05), 단백질 15%와 17%수준간에는 큰 차이가 없었다. 2. 란중은 에너지 수준에 따라서 차이가 없었으나 단백질수준이 증가할수록 증가하였지만 15%와 17%수준간에는 차이가 없었다. 3. 사료섭취량은 에너지 수준이 증가할수록 점차 감소하였다. 4. 사료요구율은 에너지 수준간에는 큰 차이가 없었지만 단백질 수준이 증가할수록 점차 개선되었다. 5. 산란 kg당 사료비는 에너지수준이 증가할수록 점차 증가하였으며 단백질수준간에는 차이가 없었다. 6. 증체량은 대시에너지 2,500kcal/kg 수준에 비하여 2,700 및 2,900kcal/kg 수준에서 증가하였고, 단백질 13%수준에 비하여 15 및 17%수준에서 증가하였다. 7. 생존율은 에너지수준이나 단백질수준에 따라 차이가 없었다. 8. 란각질은 에너지수준이 증가함에 따라 점차 떨어지는 경향이었으나 단백질수준간에는 차이가 없었다. 9. 란황차색도는 에너지수준에 따라서는 차이가 없었지만 단백질수준이 증가함에 따라 점차 증가하는 경향이었다. 10. 단백질을 제외한 모든 영양소의 이용율은 에너지 수준이 증가함에 따라 증가하였지만, 단백질수준에 따라서는 건물의 이용율만 감소하는 경향이었고 다른 영양소의 이용율은 차이가 없었다. 11. 복강지방축적율은 에너지수준이 증가할수록 점차 증가하는 경향이었다. 이상의 결과를 종합하여 볼 때 우리나라의 사료적 여건이나 환경적 여건하에서 산란계사료의 대사에너지 수준은 2,500kcal/kg, 단백질수준은 15%가 적합할 것으로 사료된다.

• Animal Bioscience
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• 제36권12호
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• pp.1880-1888
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• 2023

Objective: The present experiment aimed to determine standardized ileal digestible (SID) lysine (Lys) requirements for pregnant sows individually housed under commercial farm conditions. Methods: Two hundred multiparous sows (parity = 5.1±2.0) on day 42 of gestation were randomly allocated to five dietary treatments with a balanced parity. Experimental diets were formulated to contain 0.22%, 0.32%, 0.42%, 0.52%, and 0.62% of SID Lys for the mid-gestation period (days 42 to 76) and 0.36%, 0.46%, 0.56%, 0.66%, and 0.76% of SID Lys for the late gestation period (days 77 to 103). All indispensable amino acids except Lys were provided at 110% of their requirement estimates. Daily feed allowance per sow was determined based on the back-fat thickness and body condition score at the second pregnancy check and on day 90 of gestation. Three different statistical models were used to estimate the SID Lys requirement. Results: Total born piglets alive per litter increased linearly and quadratically (p<0.001) as dietary SID Lys increased. For total born piglets alive per litter, the SID Lys requirement estimates ranged from 9.69 to 12.4 g/d for the mid-gestation period (1.19 to 1.52 g/Mcal metabolizable energy; 0.39% to 0.49%) and 14.6 to 17.4 g/d for the late gestation period (1.62 to 1.93 g/Mcal metabolizable energy; 0.52% to 0.62%). Conclusion: The mean values of the SID Lys requirement for the mid-gestation period and the late gestation period are 11.1 and 16.1 g/d (1.36 and 1.79 g/Mcal metabolizable energy; 0.44% and 0.58%), respectively, for maximal total born piglets alive per litter.

• 대한지역사회영양학회지
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• 제25권2호
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• pp.112-125
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• 2020

Objectives: Diet and incidence of chronic diseases are highly related. This study examined the characteristics of dietary safety awareness and competency for chronic disease prevention among adults. Methods: Data were collected from 247 adults in Daegu and Gyeongbuk areas using a self-administered questionnaire in May and June of 2018. Data were analyzed by frequency analysis, χ²-test, factor analysis, reliability analysis, t-test, one-way analysis of variances, and correlation. Results: The results of the factor analysis indicate that dietary safety awareness of health management was classified into chronic disease anxiety and obsession. Awareness of dietary safety management was sub-grouped into difficulty in acquiring knowledge, lack of awareness of over and malnutrition, food safety anxiety, importance of weight management, education requirement for cancer prevention, and knowledge. Dietary safety behavior composed of a balanced diet, unhealthy diet, and health-functional pursuit. Dietary safety management competency was comprised of health management, food management, and cooking. The competency scores of dietary safety management factors were significantly different according to sex, age, and education level (P < 0.05). Balanced diet factor was significantly correlated with knowledge, health-functional pursuit, health management, food management, and cooking capacity factors (P < 0.01). Conclusions: Active education for dietary safety management competency according to age, gender, and education level should provide dietary safety education to reduce anxiety and obsession regarding chronic diseases and sustainable health management.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.261-263
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• 1989