• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1326-1335
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• 2005

The main purpose of this study is to investigate the effects of dietary lysine deficiency on protein turnover of porcine muscles. There were 18 LYD three-breed-crossing postweanling barrows from six litters cannulated with gastric tubes through the esophagus at approximate 10 kg of body weight and allocated into three treatment groups. When their body weights reached over 12 kg, one group was sacrificed for determining the initial protein masses of m. masseter, m. longissimus dorsi, m. adductor and m. biceps femoris from the right body side. The others received a diet containing 100% or 61.4% (calculated values) of the lysine requirement (NRC, 1998) multiplied by 1.103 for a period of 17 days. Daily feed provision was computed for each pig according to body weight at the same day. All pigs were infused a flooding dose of $^2$H$_5$-phenylalanine to determine the fractional protein synthesis rates (FSR) of the aforementioned muscles in the end. Their four muscles from the right body side were also dissected for measuring the fractional rates of protein accretion (FAR). As for protein degradation, fractional rates (FDR) were calculated by differences between synthesis and accretion. Results showed that the lysine deficiency resulted in, significantly (p<0.05), lighter body weights, smaller muscles and a slower growth rate. The protein mass, accreted by the muscles, of the deficient group was only 54% averaged of the pigs fed adequately (p<0.05). The FAR of these muscles in the deficient group was significantly lower (p<0.05) and only achieved 61.1% averaged of the control; there was no significant difference (p>0.05), nevertheless, in the amino-acid composition of muscles between two groups. The lysine deficiency reduced significantly (p<0.05) the FSR of m. longissimus dorsi but did not influence its FDR. The m. biceps femoris also presented an inhibited FSR while its FDR reduced only exhibited a very high tendency (p = 0.055) compared to the adequately-fed pigs. As for the m. masseter and m. adductor, both of the FSR and FDR were depressed significantly (p<0.05) by the lysine deficiency, and changes in the FSR were severer than those in the FDR, so that their FAR were significantly slower (p<0.05) in comparison with the control group. The lysine deficiency also inhibited the RNA translation activity of the muscles while the effects on RNA capacity were not significant (p>0.05). In conclusion, the FAR of muscle protein was changed by the current lysine deficiency through the alterations in the FSR and/or FDR.

• Asian-Australasian Journal of Animal Sciences
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• 제31권2호
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• pp.252-262
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• 2018

Objective: An experiment was conducted to evaluate dietary supplemental trace mineral source and deletion on mineral content in tissues. Methods: Weanling crossbred pigs (n = 144; 72 barrows and 72 gilts; body weight [BW] = $7.4{\pm}1.05kg$) were used. A basal diet was prepared, and trace mineral premix containing either inorganic (ITM) or organic (OTM) trace minerals (Cu, Fe, Mn, and Zn) was added to the basal diet. Pigs were blocked by sex and BW and randomly allotted to 24 pens for a total of 6 pigs per pen, and fed a diet containing either ITM or OTM supplemented at the 1998 NRC requirement estimates for each of 5 BW phases (Phase I to V) from 7 to 120 kg. The trace mineral supplementation was deleted for 6, 4, 2, and 0 wk of Phase V; regarding nutrient adequacy during this phase, the indigenous dietary Fe and Mn was sufficient, Cu was marginal and Zn was deficient. Results: At the end of Phase IV, Mn content (mg/kg on the dry matter basis) was greater (p<0.05) in heart (0.77 vs 0.68), kidney (6.32 vs 5.87), liver (9.46 vs 8.30), and longissimus dorsi (LD; 0.30 vs 0.23) of pigs fed OTM. The pigs fed OTM were greater (p<0.05) in LD Cu (2.12 vs 1.89) and Fe (21.75 vs 19.40) and metacarpal bone Zn (141.86 vs 130.05). At the end of Phase V, increased length of deletion period (from 0 to 6 wk) resulted in a decrease (linear, p<0.01) in liver Zn (196.5 to 121.8), metacarpal bone Zn (146.6 to 86.2) and an increase (linear, p<0.01) in heart Mn (0.70 to 1.08), liver Mn (7.74 to 12.96), and kidney Mn (5.58 to 7.56). The only mineral source by deletion period interaction (p<0.05) was observed in LD Zn. Conclusion: The results demonstrated differential effects of mineral deletion on tissue mineral content depending on both mineral assessed and source of the mineral.

• Asian-Australasian Journal of Animal Sciences
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• 제17권2호
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• pp.263-270
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• 2004

A repeated $4{\times}4$ Latin square design was conducted with eight ileal cannulated castrates to examine the effect of source of starch and fiber on nutrient balance and energy metabolism. Pigs were fed on one of the four experimental diets: Control diet (C) mainly based on cooked rice; and diets P, S and W with the inclusion of either raw potato starch, sugar beet pulp or wheat bran supplementation, respectively. With the exception of an increased (p<0.05) energy loss from methane production with diet S observed, no significant differences (p>0.05) in the ratio of metabolizable energy (ME)/digestible energy, the utilization of ME for fat deposition and for protein deposition, energy loss as hydrogen and urinary energy were found between diets. The efficiency of utilization of ME for maintenance was lower (p<0.05) with diets P and S than with diet C. The inclusion of fiber sources (sugar beet pulp or wheat bran) or potato starch reduced the maintenance energy requirement. The fecal energy excretion was increased (p<0.05) with either sugar beet pulp or wheat bran supplementation, while it was unaffected (p>0.05) by addition of potato starch. In comparison with diets C and P, a lowered ileal or fecal digestibility of energy with diets S and W was observed (p<0.05). Feeding sugar beet pulp caused increased (p<0.05) daily production of methane and carbon dioxide and consequently increased energy losses from methane and carbon dioxide production, while it did not influence the daily hydrogen production (p>0.05). An increased (p<0.05) proportion of NSP excreted in feces was seen by the supplementation of wheat bran. Higher NSP intake caused an increased daily amount of NSP in the ileum, but the ileal NSP proportion as a percentage of NSP intake was unaffected by diets. Feeding potato starch resulted in increased daily amount of starch measured in the ileum and the proportion of ileal starch as a percentage of starch intake, while no significant influence on fecal starch was found. Higher (p<0.05) daily amount of fecal starch and the proportion of fecal starch as a percentage of starch intake were found with fiber sources supplementation compared with diets C and P. By increasing the dietary NSP content the fecal amount of starch increased (p<0.01).

• 한국가정과학회지
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• 제9권3호
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• pp.69-79
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• 2006

The previous studies on the intake of trace elements performed in Korea were only concerned about major elements like Fe, Zn or Cu. There is little data about the intake of minor elements like Mn, Se, Mo or Cr. And most of the data were obtained by calculation using Food Composition Tables or by analysis using atomic absorption spectrophotometers (AAS). The purpose of this study, therefore, was to evaluate the dietary intake of seven trace elements, Fe, Zn, Cu, Mn, Se, Mo, and Cr of Korean adult wonmen and to compare the data between analyzed using ICP-MS (or ICP-AES) and calculated by the CAN-Pro (or Food Composition Table). A total of nineteen adult women participated voluntarily in this study and collected one-tenth of the foods that they consumed for three consecutive days. Analyzed intake of Fe of the subjects was $6.94{\pm}2.18$ (calculated, $18.87{\pm}4.50$) mg/day, that of Ze was $9.35{\pm}4.95$ (calculated, $8.35{\pm}2.87mg/day$), that of Cu was $1.18{\pm}0.26\;(1.11{\pm}0.32mg/day)$, that of Mn was $3.69{\pm}0.69\;(2.83{\pm}1.68mg/day)$, that of Se was $41.93{\pm}9.28$ (calculated, $27.58{\pm}6.97{\mu}g/day$), that of Mo was $134.0{\pm}49.1{\mu}g/day$, and that of Cr was $136.5{\pm}147.9{\mu}g/day$. The analyzed Fe intake of the subjects did not meet Estimated Average Requirement (EAR) nor Recommended Intakes (RI) for Korean women aged 20-29 years old. However, the analyzed intakes of the other six elements, Zn, Cu, Mn, Se, Mo, and Cr, seemed to meet each of the respective RIs. The analyzed Fe intake was significantly lower than the calculated one, in fact by 2.72 times, however, the analyzed intakes of the other elements, Mn and Se, were significantly higher and those of Zn and Cu were similar than each of the calculated ones. The difference of the data between analyzed and calculated intakes indicates that it is necessary to set up database on trace element contents of foods of the Food Composition Table and the CAN-Pro so as to have accuracy.

• 한국식품영양과학회지
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• 제42권6호
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• pp.904-910
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• 2013

본 연구는 젊은 성인을 대상으로 운동 목적에 따른 영양섭취 실태를 조사하고자 수행되었다. 규칙적으로 운동하고 있는 20~30대 남녀를 대상으로 운동 목적에 따라 근육증가군 107명과 체중감소군 107명을 나누어 비교 분석하였다. 그룹별 성별구성을 분석한 결과, 근육증가군은 주로 남성(75.2%)이 많은 반면 체중감소군은 여성(74.3%)이 많이 분포하고 있었다. BMI 판정결과 두 그룹간의 유의적인 차이는 없었으며, 체중감소군의 절반 이상(56%)이 저체중 또는 정상체중으로 나타났다. 근육증가군의 하루 운동시간 및 규칙적으로 운동한 기간이 체중감소군에 비하여 유의적으로 많았다(p<0.01). 영양지식의 상태는 근육증가군의 점수가 체중 감소군에 비하여 유의적으로 높은 것으로 나타났다(p<0.05). 식행동 또한 근육량 증가군의 점수가 체중감소군에 비하여 유의적으로 높았다(p<0.05). 영양섭취 상태를 살펴보면, 한국인영양섭취기준과 비교하였을 때 근육증가군의 에너지 섭취비율이 에너지필요추정량의 83.72%로 체중감소군의 74.53%에 비하여 유의적로 높았다(p<0.01). 권장섭취량에 대한 단백질 섭취비율은 근육증가군이 199.62%, 체중감소군은 136.36%로 나타났으며 근육증가군이 체중감소군에 비해 유의적으로 매우 많은 양을 섭취하고 있었다(p<0.001). 권장섭취량에 대한 칼슘 섭취비율은 근육증가군이 67.75%, 체중감소군은 68.64%로 조사되었으며, 두 군 모두 권장섭취량과 비교하여 매우 부족한 수준으로 나타났다. 이와 같이 본 연구에서는 근육증가를 목적으로 운동하는 경우 과량의 단백질을 섭취하고 있었으며, 체중감소를 목적으로 운동하는 경우 필요량에 미치지 못하는 에너지 섭취를 하는 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권11호
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• pp.1616-1624
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• 2016

The aim of present experiment was to investigate the effect of protein reduction in commercial broiler chicken rations with incorporation of de-oiled rice bran (DORB) and supplementation of limiting amino acids (valine, isoleucine, and/or tryptophan) with ration formulation either on total amino acid (TAA) or standardized ileal digestible amino acids (SIDAA). The experimental design consisted of $T_1$, TAA control; $T_2$ and $T_3$, 0.75% and 1.5% protein reduction by 3% and 6% DORB incorporation, respectively by replacing soybean meal with supplemental limiting amino acids to meet TAA requirement; $T_4$, SIDAA control, $T_5$ and $T_6$, 0.75% and 1.5% protein reduction by DORB incorporation (3% and 6%) with supplemental limiting amino acids on SIDAA basis. A total of 360 dold fast growing broiler chicks (Vencobb-400) were divided into 36 homogenous groups of ten chicks each, and six dietary treatments described were allocated randomly with six replications. During 42 days trial, the feed intake was significantly (p<0.05) reduced by TAA factor compared to SIDAA factor and protein factor significantly (p<0.05) reduced the feed intake at 1.5% reduction compared to normal protein group. This was observed only during pre-starter phase but not thereafter. The cumulative body weight gain (BWG) was significantly (p<0.05) reduced in TAA formulations with protein step-down of 1.5% ($T_3$, 1,993 g) compared to control ($T_1$, 2,067 g), while under SIDAA formulations, BWG was not affected with protein reduction of 1.5% ($T_6$, 2,076 g) compared to $T_4$ (2,129 g). The feed conversion ratio (FCR) was significantly (p<0.05) reduced in both TAA and SIDAA formulations with 1.5% protein step-down ($T_3$, 1.741; $T_6$, 1.704) compared to respective controls ($T_1$, 1.696; $T_4$, 1.663). The SIDAA formulation revealed significantly (p<0.05) higher BWG (2,095 g) and better FCR (1.684) compared to TAA formulation (2,028 g; 1.721). Intake of crude protein and all limiting amino acids (SID basis) was higher in SIDAA group than TAA group with resultant higher nitrogen retention (4.438 vs 4.027 g/bird/d). The nitrogen excretion was minimized with 1.5% protein reduction (1.608 g/bird) compared to normal protein group (1.794 g/bird). The serum uric acid concentration was significantly reduced in $T_3$ (9.45 mg/dL) as compared to $T_4$ (10.75 mg/dL). All carcass parameters were significantly (p<0.05) higher in SIDAA formulation over TAA formulation and 1.5% protein reduction significantly reduced carcass, breast and thigh yields. In conclusion, the dietary protein can be reduced by 0.75% with TAA formulation and 1.5% with SIDAA formulation through DORB incorporation and supplementation of limiting amino acids and among formulations, SIDAA formulation was better than TAA formulation.

• 한국수산과학회지
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• 제29권5호
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• pp.651-662
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• 1996

여러 종류의 단백질 원료를 혼합하여 성장이 좋고, 가격이 싼 경제적인 조피볼락용 배합사료를 개발하기 위해 실험사료의 효과를 사육실험과 사료 및 어체의 성분 분석을 통해 조사하였다. 북양어분 사료를 대조구 (사료 1)로 하고 사료 원료의 아미노산 조성을 고려하면서 대두박, 콘글루텐 밀, 육분, 혈분, 우모분, 소맥분을 서로 다른 비율로 첨가한 9종류의 실험사료를 설계하여 제조하였다. 사료 2에서 5까지는 조피볼락 전어체의 아미노산 조성, 타 어종을 대상으로 연구된 아미노산 요구량 등을 참고로 아미노산 균형을 고려하여 설계하였다. 사료 6은 복합 효소제의 효과를 알아보기 위하여 사료 5의 조성에서 복합 효소제를 첨가하지 않았고, 사료 7은 합성 아미노산 첨가 효과를 검토하기 위해 사료 5의 조성에서 아미노산을 첨가하지 않았다. 그리고 사료 8과 9는 열처리한 대두박과 옥수수를 첨가하여 그 효과를 비교하였다. 114g 전후의 조피볼락을 사료당 2반복으로 15주간 사육실험한 결과, 증체율과 사료효율에 서 사료 $2\~7$구가 대조구와 유의차없이 (P>0.05) 좋은 것으로 나타났으며, 사료 5의 조성에서 합성 아미노산이나 복합효소제를 첨가하지 않아도 성장차이는 없었다. 어체 1kg 생산하는데 소요되는 사료단가를 계산하여 본 결과, 대조사료는 853원이었고, 사료 2와 7이 $732\~746$원으로 낮았으며, 사료 8과 9가 $875\~905$원으로 높았다. 위의 결과로부터 어체크기가 100g 이상일 경우의 사료에 대두박, 콘글루텐 밀, 육분, 혈분, 우모분, 소맥분을 적절히 첨가하면 북양어분을 $50\%$ 정도까지 대체 가능할 것으로 판단되어 육성용 사료의 단가를 낮출 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제10권5호
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• pp.445-454
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• 1997

Rice bran commonly available in Bangladesh is a mixture of rice hulls (60%), bran (35%) and polishing (5%), referred here as rice mill feed (RMF). Dose response effect of RMF supplementation to a straw diet including a zero level was measured on the intake, digestibility, nitrogen balance, microbial N yield and growth rate of growing native (Bos indicus) bulls. Twelve bulls of 33 months old and $272{\pm}31.5kg$ weight were randomly allocated to diets having 0 (T1), 1 (T2) and 2 (T3) kg RMF in addition to 200 g wheat bran, 200 g molasses, 60 g salt and 30 g oyestershe\l powder. Concentrate intake was 5.5, 19.2 and 29.5% of the dietary intake for the T1, T2 and T3 treatment respectively. RMF supplementation had no significant effect on the straw DM intake. However, with the increasing levels of RMF supplementation, total DM & digestible OM intake and the whole gut digestibilities of DM, OM, N & ADF increased but in deminishig return. Total microbial N yield estimated from the urinary purine excretion were 15.35, 26.56 and 38.44 g/d for the treatment T1, T2 and T3 respectively. Both the N intake and the N balance increased linearly in response to increasing level of RMF. Supplementation of RMF linearly increased the energy intake and dietary energy concentration. Growth rate in the T1, T2 and T3 treatments were 112, 125 and 250 g/d respctively. The basal N excretion and the maintenance energy requirement of the experimental animals were estimated to be 615 mg/kg $W^{0.75}/d$ and 447 kJ/kg $W^{0.75}/d$ respectively. The estimated efficiency on N utilization was 0.83 mg/mg of N intake ($r^2=0.997$) while the efficiency of metabolizable energy utilization for growth was 0.15. Since animal refused higher levels of RMF, inclusion up to 2 kg level (about 25% of the total DM intake) appears to have no depressing effect on the performances of animal. However, RMF itself fail to meet the critical nutrient need of the rumen microbes. Therefore response of supplementing RMF after correcting the critical nutrient deficiency need to be studied.

• 한국식생활문화학회지
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• 제21권5호
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• pp.473-480
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• 2006

The purpose of this study were to investigate middle and high school students' food habits and food attitudes, and to assess student's nutrient consumption. A self-administered questionnaire was developed based on review of literature. The questionnaire consisted of three sections (food habits, food attitudes, and food consumption). The questionnaires were distributed to 4,050 students enrolled in 34 middle and high schools located in Seoul, Gyeonggi, and Gyeongnam provinces. A final response rate was 88.2% (3,570) excluding responses that had significant missing data. Data of the food habits and food attitudes were analyzed with descriptive analysis, $x^2$-test, and t-test using SPSS WIN(ver.11.0). The student's self-reported food consumption data was converted into nutrient consumption using conversion factor. Many middle and high school students skipped breakfast and/or dinner. Approximately 29% of the students did not eat vegetables and fruits. Scores of the middle school student's attitudes(19.1) towards foods were significantly higher than those of the high school student's attitudes(18.7)(p<.001). Student's nutrient intakes were estimated according to the student's self-reported food consumption data. The nutrient intakes were compared with DRIs(Dietary Reference Intakes: DRIs) for their age groups. The result of EAR(Estimated Average Requirement: EAR) cut-point method demonstrated 57.7% of middle school boys, 64.4% of middle school girls, 70.2% of high school boys, and 71.0% of high school girls did not meet EAR for Calcium. Additionally, it showed that 39.0% of middle school boys, 23.7% of middle school girls, 58.4% of high school boys, and 24.4% of high school girls did not consume EAR for Vitamin $B_1$. 25.7% of middle school boys did not meet EAR for Vitamin $B_2$, while 44.4% high school boys did not satisfy EAR for Vitamin $B_2$.

• 한국가금학회지
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• 제21권1호
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• pp.41-48
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• 1994

본(本) 연구(硏究)에서는 난용계(卵用鷄) 산란기(産卵期)의 self-selection diets를 개발하기 위한 기초자료를 얻기 위하여 각종 선택채식(選擇採食) 방법(方法)에 의해 산란기의 사료(飼料) 및 영양소(營養素) 섭취형태(攝取形態)를 조사하였다. 선택채식시험을 위하여 옥수수사료, 대두박사료, 밀기울사료, 대두박+어분사료 그리고 석회석사료를 각각 만든 후 단일배합사료(單一配合飼料)를 급여(給與)하는 대조구(對照區)(C)와 옥수수-대두박-석회석사료(飼料) 선택채식구($T_1$), 옥수수-대두박-밀기울-석회석사료(飼料) 선택채식구($T_2$), 옥수수-대두박+어분-밀기울-석회석사료(飼料) 선택채식구($T_3$)등 4개처리에 유색산란계(有色産卵鷄)를 처리당 10수씩 총 40수를 공시(供試)하여 31주령과 41주령에 2회에 걸쳐 각 1주일간의 본(本) 시험(試驗)을 실시한 결과(結果)는 다음과 같다. 1. 처리별 산란률(産卵率)은 73.02~79.83%로 큰 차이는 없었으며, 1일중 시간대별(時間帶別) 산란분포(産卵分布)는 처리간에 큰 경향의 차이없이 07-13시의 오전시간에 대부분의 산란되었다. 2. 각 처리 공히 계란형성일(鷄卵形成日)에는 비형성일(非形成日)에 비해 사료(飼料) 및 영양소(營養素) 섭취량(攝取量)이 많았으며, 계란형성일에는 선택채식구(選擇採食區)들이 대조구(對照區)에 비해 에너지와 단백질(蛋白質) 섭취량(攝取量)이 적었다. 3. 계란형성일의 시간대별 사료(飼料)와 에너지 및 단백질섭취량(蛋白質攝取量)은 대조구(對照區)가 10시 이후부터 22시까지 계속 증가하였으나, 선택채식구(選擇採食區)들은 10시 이후부터 22시까지 계속 증가하였으나, 선택채식구(選擇採食區)들은 10시 이후부터 증가하다가 19~22시의 마지막 3시간 동안의 섭취량(攝取量)이 감소하였다. 4. 계란 형성일의 시간대별 칼슘섭취량(攝取量)은 대조구(對照區)가 10시 이후부터 22시까지 계속 완만하게 증가하였으며, 선택채식구(選擇採食區)은 16시 이전까지는 대조구(對照區)보다 훨씬 적었으나 16시 이후에는 급격히 증가하여 대조구(對照區)보다 훨씬 많이 섭취(攝取)하였다. 결론적으로 난용계(卵用鷄) 산란기에는 영양소요구량(營養素要求量)의 주기적인 변화에 대처할 수 있는 복수선택채식사료(複數選擇採食飼料)의 개발이 필요하다고 생각된다.