• 대한약침학회지
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• 제22권3호
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• pp.122-130
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• 2019

Objectives: The medicinal plants are believed to enhance the natural resistance of the body to infections. Some of the main constituents of the plant and derived materials such as, proteins, lectins and polysaccharides have anti-inflammatory effects. Portulaca oleracea (P. oleracea) were used traditionally for dietary, food additive, spice and various medicinal purposes. This review article is focus on the anti-asthmatic effects of P. oleracea and its constituents. Methods: Various databases, such as the PubMed, Scopus, and Google Scholar, were searched the keywords including "Portulaca oleracea", "Quercetin", "Anti-inflammatory", "Antioxidant", "Cytokines", "Smooth muscle ", and " Relaxant effects " until the end of Jul 2018. Results: P. oleracea extracts and its constituents increased $IFN-{\gamma}$, IL-2, $IFN{\gamma}/IL-4$ and IL- 10/IL-4 ratio, but decreased secretion of $TNF-{\alpha}$, IL-4 and chemokines in both in vitro and in vivo studies. P. oleracea extracts and quercetin also signifcantly decreased production of NO, stimulated ${\beta}$-adrenoceptor and/or blocking muscarinic receptors in tracheal smooth muscles. Conclusion: P. oleracea extracts and quercetin showed relatively potent anti-asthmatic effects due to decreased production of NO, inflammatory cytokines and chemokines, reduced oxidant while enhanced antioxidant markers, and also showed potent relaxant effects on tracheal smooth muscles via stimulatory on ${\beta}$-adrenoceptor or/and blocking muscarinic receptors.

• 한국미생물·생명공학회지
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• 제49권1호
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• pp.18-23
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• 2021

The aim of this study was to determine the production of folate, short chain fatty acids (SCFAs), and antimicrobial activity exhibited by Lactobacillus plantarum G72 for potential dietary application in pregnant women. L. plantarum G72 has been reported to possess characteristic activities and functionality including β-galactosidase activity and antioxidant activities. L. plantarum G72 showed antibacterial activity against pathogenic bacteria (Listeria monocytogenes ATCC 15313, Salmonella typhimurium P99, Escherichia coli ATCC 25922, and Staphylococcus aureus KCCM 11335) using a modified method, and formation of the largest inhibition zone was observed against S. aureus KCCM 11335 (12.0-17.0 mm). The adherence of four food-borne pathogenic bacteria to HT-29 cells was inhibited by L. plantarum G72 (0.13 to 0.92 log CFU/ml). The most considerable inhibition of adherence to HT-29 cells was observed by using L. plantarum G72 against S. typhimurim P99. Additionally, folate production by L. plantarum G72 was 50.1 ng/ml, and L. plantarum G72 produced relatively more lactic acid (11,176.73 mg/kg) than acetic, propionic, or butyric acids. Therefore, the results of this study suggest that L. plantarum G72 may serve as a multifunctional food additive in the health industry.

• 한국가금학회지
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• 제35권3호
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• pp.275-281
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• 2008

산란계 사료에 lycopene을 첨가 급여하여 생산된 계란의 난황 중에 전이된 lycopene의 함량을 급여 기간별로 확인하고, lycopene 함유 계란의 난황색, 난중, 난각 강도, 난황고, 난백고, 난황 직경, 난황 계수와 Haugh Unit(호우 유닛) 등 계란의 각종 품질 특성과 이들 계란의 장기 보존에 따른 품질의 변화상을 조사하여 다음과 같은 결과를 얻었다. 1. 결과 lycopene 처리군의 계란 중 lycopene 농도는 난황액 1 gram 당 각각 약 1.56 ${\mu}g$과 1.57 ${\mu}g$ 이었으며, 대조군의 계란에는 lycopene의 함량이 없는 것으로 나타났다. 2. 사양 시험 2주경의 처리군의 난황 중 lycopene 함량 수준(23.4 ${\mu}g$)은 4주경의 수준(23.6 ${\mu}g$)과 유사하였다. 즉, 동일수준의 lycopene 첨가 사료를 지속적으로 급여하여도 난황 내 lycopene의 함량은 급여 기간의 경과에 따라 누적적으로 증가되지 않는 것으로 나타났다. 3. Lycopene 첨가군의 난황색 (P<0.01)은 대조군에 비하여 유의적으로 높은 수치를 보였다. 이외에도 처리군간에 통계적 유의성은 인정되지 않았으나 lycopene 첨가군의 난황고(P<0.08), 난황 직경(P<0.19), 난각 견실도(P<0.19), 호우 유닛(P<0.34), 난백고(P<0.33)도 대조군에 비하여 다소 높은 수치를 보였다. 각 처리군간에 난중의 차이는 없는 것으로 나타났다. 4. 계란을 실온에서 4주간 보관시킨 후, 이들의 품질을 측정한 결과, Lycopene 처리군의 난황색(EYC)은 대조군에 비하여 유의적으로 높았으나(P<0.01), 그 이외의 항목에서는 유의적 차이가 없었다. 저장 4주 후의 첨가군의 난백고와 난황고는 대조군 보다 크고 난황 직경(EYD)은 대조군보다 작게 나타났다. 이 현상은 첨가군의 호우유닛 수치를 대조군보다 크게 하는 데 영향을 미친 것으로 추정된다.

• 한국수산과학회지
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• 제39권6호
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• pp.460-465
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• 2006

An 8-week feeding trial was conducted to investigate the effects of dietary supplementation with probiotics as a feed additive for Juvenile olive flounder (Paralichthys olivaceus). Three experimental diets supplemented with Bacillus polyfermenticus (BP), Bacillus licheniformis (BL), or Bacillus polyfermenticus plus Saccharomyces cerevisiae, (BP+SC) at $1.0{\times}10^7CFU/kg$ diet on a dry-matter basis were prepared. The basal diet was used as a control. After the 8-week feeding trial, the respiratory burst activity (NBT assay) of fish fed the BP + SC diet was significantly higher than that of fish fed the control diet. Fish fed the BP, BL and BP + SC diets had significantly lower cumulative mortality than did fish fed the control diet after the third day of the challenge test (P<0.05). However, there were no significant differences among fish fed the experimental diets in weight gain, feed efficiency, protein efficiency ratio, hematosomatic index, condition factor, survival rate, or Iysozyme activity. Results could suggest that dietary B. polyfermenticus, B. licheniformis, and B. polyfermenticus +S. cerevisiae enhance nonspecific immunity and disease resistance in juvenile olive flounder.

• 한국수산과학회지
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• 제46권5호
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• pp.559-564
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• 2013

This study was performed to determine effects of dietary inclusion of various concentrations and sources of sea tangle Laminaria japonica on growth, body composition and plasma chemistry of juvenile black porgy Acanthopagrus schlegeli. Juvenile fish (N=810; average mass=8.9 g) were distributed among twenty-seven 200 L flow-through tanks (30 fish per tank). Nine experimental diets were prepared: control (Con) without additives; sea tangle powder at 0.1%, 0.5%, 1%, 2%, 3% and 5%, (P-0.1, P-0.5, P-1, P-3 and P-5 diets, respectively); and fresh sea tangle (F-1); and 1% sea tangle extract (E-1). Each powdered additive was substituted for an eqaul amount of wheat flour, and the extract was substituted for an equal amount of water in the respective experiemtnal diets. Each diet was fed to triplicate groups of fish. Weight gain and feed conversion ratio were not affected by the experimental diets. Feed consumption by fish fed the P-1 diet was higher than that of fish fed the P-3 and P-5 diets. Moisture, crude protein, crude lipid, whole-body ash content (excluding the liver), liver moisture, and crude protein and lipid contents of fish were not affected by the experimental diets. None of the fish plasma criteria were affected by the experimental diets, with the exception of plasma glucose. In conclusion, dietary inclusion of various concentrations and sources of sea tangle did not significantly affect the growth, feed efficiency, body composition or plasma chemistry of juvenile black porgy.

• 한국수산과학회지
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• 제46권5호
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• pp.552-558
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• 2013

This study was performed to determine effects of the supplementation of Kimchi lactic acid bacterial culture in extruded pellets (EP) on the growth, body composition, blood chemistry and immune response of olive flounder Paralichthys olivaceus. Four hundred eighty individuals averaging 16.1 g were randomly distributed into 12, 180 L flow-through tanks (forty fish per tank). Four concentrations of Kimchi lactic acid bacterial culture (KL) were prepared: Control (0%), 0.1%, 0.2% and 0.5%. Three concentrations (0.1%, 0.2% and 0.5%) of Kimchi lactic acid bacterial culture were each diluted to 10% of EP weight and then fully absorbed by EP for 10 minutes. Each diet was fed to triplicate groups of fish. Fish were hand-fed to apparent satiation twice a day for 8 weeks. At the end of the 8-week feeding trial, the plasma lysozyme and bacterial activities of fish were determined. In addition, the cumulative mortality of fish was monitored for 8 days after their artificial infection with Edwardsiella tarda. The weight gain, specific growth rate, feed efficiency ratio, protein efficiency ratio, protein retention, hepatosomatic index and condition factor of fish were not affected by dietary supplementation with KL. None of the proximate composition, plasma parameters, lysozyme or bactericidal activities of fish was affected by dietary supplementation with KL. However, the cumulative mortalities of fish fed EP containing 0.1% and 0.5% Kimchi lactic acid bacterial culture were relatively low compared to that of fish fed the control diet. In conclusion, dietary supplementation with KL did not effectively improve growth, feed utilization, body composition, plasma chemistry, lysozyme, bactericidal activities or immune response of olive flounder after E. tarda infection under these experimental conditions.

• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1096-1103
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• 1999

A study was conducted to clarify the impact of recombinant porcine somatotropin (pST) on the efficiency of absorbed nitrogen use for protein deposition in growing pigs. Three levels of dietary crude protein (9.0, 11.5, 14.0% CP) were used. Each had either a sub-optimum or near optimum lysine: CP concentration (Low-lysine, 3.8 g/100 g CP and High-lysine, 5.5 g/100g CP) in order to achieve different metabolic efficiencies for nitrogen deposition (ca. 45 vs. 60%). Twelve crossbred female pigs $(59{\pm}4kg\;BW)$ were placed in metabolism cages and fitted with bladder catheters. Each pig received an excipient injection daily for the first 10-d, a pST (5 mg/d) injection for the second 10-d, and then excipient for the last 10-d. Pigs were randomly assigned to one of six dietary treatments (2 pigs/diet) and fed 4 times per d at $92g/kg\;BW^{0.75}$ $(3{\times}maintenance)$. Means for the excipient period were compared to means for the pST period. Urinary nitrogen (N) output declined in pST-treated pigs (p<0.01) irrespective of dietary protein content or lysine level. Nitrogen retention increased by an average of 11% (p<0.01) with pST treatment (726 vs. $803mg\;N/kg^{0.75}\;BW/d$). Forty-eight percent of the absorbed N was retained with Low-lysine diets, but this increased to 53% with pST injection (+11%, p<0.01). Pigs fed High-lysine diets retained 62% of absorbed N which increased to 69% with pST (+11% p<0.01). the addition of lysine improved N use by 27% (High vs. Low, p<0.01), but the effect of lysine and pST was additive (+40%). Therefore, pST improves N retention and the efficiency of apparently absorbed N use in growing pigs (>60kg). It does so with diets having the potential for either low or high efficiencies of N use (48% and 62%). More work is needed to determine if the partial efficiency of N use improves in direct proportion to pST dose since the improvement in protein deposition is a function of pST dose.

• 한국축산시설환경학회지
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• 제17권sup호
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• pp.35-42
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• 2011

본 시험은 사료내 phytase 첨가가 육성비육돈의 생산량과 분뇨 배설량에 미치는 영향을 조사하기 위해 수행하였다. 3원 교잡종 (Yorkshire ${\times}$ Landrace ${\times}$ Duroc) 육성비육돈 80수를 공시하였으며, 처리구는 phytase 첨가 유무에 따른 2처리구, 처리구당 5반복, 반복당 8수씩을 육성비육돈의 체중 (50, 80, 110 kg)에 따라 3단계로 나누어 phytase 첨가 유무 (0, 500 FTU/kg)와 육성 비육돈 성장 3단계 (육성I기, 육성II기, 비육기)의 $2{\times}3$ 복합요인으로 7일간씩 수행하였다. 육성비육돈의 성장 단계에 따른 개시체중은 각각 $58.6{\pm}3.9$, $83.2{\pm}3.8$ 및 $111.4{\pm}5.4kg$ 이었다. 증체량은 phytase 첨가구가 무첨가구에 비해 높았으며 (P<0.01), 성장 단계별로 보면 육성II기의 증체량이 가장 높고, 비육기의 증체량이 가장 낮았다(P<0.05). 사료요구율은 phytase 무첨가구가 첨가구에 비해 높게 나타났으며, 비육기의 사료요구율이 가장 높았다 (P<0.05). 사료섭취량과 음수량은 성장 단계와 phytase 첨가 유무에 따른 섭취량의 유의적인 차이는 보이지 않았다 (p>0.05). 분의 배설량은 성장 단계에 따라 감소하였으며 (p<0.05), phytase 첨가구가 무첨가구에 비해 감소하였다 (p<0.01). 뇨의 배설량은 처리구간 유의적인 차이는 없었다. 질소와 인의 섭취량은 phytase 첨가 유무와 성장 단계에 따른 차이를 보이지 않았다(P>0.05). 질소의 배설량은 phytase와 성장 단계에 따른 차이가 없었으나, 인의 배설량은 phytase 첨가구에서 감소하였다. 질소와 인의 배설율은 phytase 첨가구가 무첨가구에 비해 감소하였다 (P<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.877-883
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• 2006

A feeding trial was conducted on commercial broilers for a period of 35 days to determine the individual and combined effects of aflatoxin (AF) and T-2 toxin (T-2) on performance, organ weights and immune status. The efficacy of dietary glucomannan-containing yeast product (GYP) ($Mycosorb^{(R)}$) and hydrated sodium calcium aluminosilicate (HSCAS) in preventing the adverse effects of aflatoxin and T-2 toxin was also evaluated. Twelve dietary treatments ($4{\times}3$ factorial) comprising two dietary levels each of AF (0 and 2 mg/kg), T-2 toxin (0 and 1 mg/kg), GYP (0 and 1 kg/ton) and HSCAS (0 and 10 kg/ton) were tested on 720 commercial broiler chickens divided at random into 36 replicates of 20 chicks each (10 males and 10 females). Weight gain and feed intake were recorded weekly. Organ morphology and antibody titers for Newcastle disease (ND) and infectious bursal disease (IBD) were measured on the $35^{th}$ day. AF and T-2 toxin individually decreased weight gain and increased feed conversion ratio (FCR) (p<0.05). AF alone (p<0.05) increased weights of liver, kidney, gizzard and spleen and reduced thymus and bursal weights. T-2 toxin (p<0.05) increased liver and gizzard weights and decreased thymus weight. Both AF and T-2 toxin when fed individually affected ND and IBD titers in a significant manner. Significant interactions between AF and T-2 toxin were observed for their additive effects on weight gain, FCR, organ weights and antibody titers. Addition of GYP (p<0.05) improved weight gain, feed conversion efficiency and restored the organ weights. Antibody titers against ND and IBD were significantly improved with the supplementation of GYP. Supplementation of HSCAS (p<0.05) resulted in improvement in weight gain and restored organ weights in the groups fed AF alone, but not in T-2 toxin fed groups. HSCAS inclusion did not influence FCR in toxin fed groups. Addition of HSCAS (p<0.05) improved the antibody titers against ND and IBD only in AF fed groups. Thus, the results indicate that addition of GYP is effective in averting the individual and combined toxicity of aflatoxin and T-2 toxin in commercial broilers, while HSCAS is effective only against aflatoxin.

• 한국양식학회지
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• 제22권1호
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• pp.112-117
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• 2009

배합사료에 생약재 첨가가 치어기 및 육성기 조피볼락의 성장, 사료이용성 및 체조성에 미치는 영향을 조사하기 위하여, 14가지 생약재 혼합물 및 약쑥과 삼지구엽을 혼합 첨가한 배합사료로 치어기(3.6 g) 및 육성기(166 g) 조피볼락을 8주간 사육실험 하였다. 치어의 생존을, 증중량, 사료 효율, 일일사료섭취량, 단백질 효율, 비만도 및 간중량지수는 사료 침가제에 영향을 받지 않았다(P>0.05). 육성어의 증중량, 일일사료섭취량, 비만도 및 간중량지수는 사료 첨가제에 영향을 받지 않았지만 (P>0.05), 사료 효율과 단백질 효율은 생약재 첨가구가 대조구보다 높은 값을 보였다. 사육실험 종료 후, 치어와 육성어의 등 근육, 간, 장 및 전어체의 일반성분 및 지방산은 모든 실험구간에 통계적인 차이가 없었다(P>0.05). 이상의 결과로부터 조피볼락 치어의 성장 및 사료이용효율이 사료 첨가제에 영향을 받지 않았지만, 육성어의 경우에는 생약재 첨가가 사료이용효율을 개선시킬 수 있을 것으로 판단되며, 어류의 성장단계에 따라 그 효능이 달라질 수 있음을 알 수 있다.