• The Journal of Internal Korean Medicine
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• v.27 no.4
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• pp.791-797
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• 2006

Objectives : The objective of the study was to observe the effects of different treatment lengths of Rhizoma Alismatis on diabetes mellitus. Methods : Rats were divided into three groups: normal(non-treated group), control (group administered saline for 4, 11, and 18 days), and RA (group administered 2.45mg/200g Rhizoma Alismatis for 4, 11, and 18 days). The experimental results were derived from the measurement of the levels of glucose, ALP, AST, ALT, creatinine and BUN in the serum from the rats on days 4, 11, and 18. Results : The glucose level in the serum significantly decreased on the 18th day. The ALP level in the serum did not show statistical significance. The GOT and GPT levels in the serum slightly decreased on the 4th, 11th, and 18th days, but did not show statistical significance. The creatinine level in the serum was unchanged for all of them. The BUN level in the serum did not show statistical significance. Conclusions : According to the above results, it is concluded that Rhizoma Alismatis has a therapeutic effect on diabetes mellitus with a longer period ofintake. As to influence upon the liver and kidney, there was no damage orside effects.

• The Journal of Korean Medicine
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• v.19 no.1
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• pp.109-126
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• 1998

This experiment was performed in order to investigate the effect of Rosa rugosae Radix on hyperglycemia and diabetes in streptozotocin-induced rats. In this study, the experimental rats were divided into three groups: Control group, Group-A(Aqua-acupuncture group) and Group-B(Orally administ:ered group). Under the same condition, three groups were injected 50mg/kg of streptozotocin into abdomen for 3days. And then, Control group was administered water, Group-A was administered Rosa rugosae Radix aqua-acupuncture to S36, B20 and B21. Group-B was administered Rosa rugosae Radix water extract for 14days. Then kidney weight, serum glucose levels, BUN levels, creatinine levels and Langerhans islets were measured on 7th and 14th days. The obtained results are summarized as follows: 1. The kidney weight of control group was heavier than those of group-A and group-Bon 7th day. 2. The levels of serum glucose were decreased in group-A and group-B at l20minutes 14th day as compared with control group. 3. The levels of BUN were decreased in group-A and group-B as compared with control group. 4. In histological observations, Langerhans islets of control group showed disrupted or condensed nudei, and intercellular spaces between acini were enlarged. In the group-A and group-B, histological damages of the acini and Lagerhans islets were recovered as compared with control group. These results suggest that Rosa rugosae Radix can applied for the treatment of diabetes melitus.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.2
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• pp.117-123
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• 1992

The present study was undertaken in order to elucidate the effects of pretreatment with nicotinamide on changes in serum glucose level, body weight, water consumption, serum insulin concentration, and the activity of pancreatic enzyme in rats treated with streptozotocin (STZ). Histological studies were also carried out to evaluate the effects on pancreatic tissues and Langerhans's islet cells. Nicotinamide pretreatment in STZ diabetic rats inhibited the rise of fasting serum glucose concentration and water consumption. Pretreatment with nicotinamide significantly increased the concentration of serum insulin and body weight changes compared to the STZ-treated group. Pancreatic lipase and trypsin activities were increased, but amylase activity was decreased and pancreatic $\beta$ -cell was destroyed by STZ. Pvetreatment with nicotinamide prevented these STZ-induced changes. These results suggest that nicotinamide pretreatment supresses STZ-induced changes in pancreatic enzymes by preventing $\beta$-cell destruction and therefore maintaining a normal serum insulin revel.

• Biomolecules & Therapeutics
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• v.7 no.1
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• pp.7-13
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• 1999

The stimulatory effect of recombinant human basic fibroblast growth factor (bFGF) on wound healing was evaluated in healing-impaired animal models. Full-thickness wounds were made in prednisolone-treated mice, streptozotocin (STZ)-induced diabetic rats and mitomycin C (MMC)-treated rats. Saline or bFGF at a dose of 1, 5, or $25\mu\textrm{g}$ per wound was applied to the open wound once a day for three to five days. The degree of wound healing was assessed using wound size and histological parameters such as degree of epidermal and dermal regeneration. Local application of bFGF accelerated wound closure significantly in a dose-dependent manner in all healing-impaired wounds (p<0.05). The wound healing effect of bFGF was further confirmed by histological examination in MMC-treated rats. Epidermal and dermal regeneration were enhanced in bFGF-treated wounds with a dose-related response. Dermal regeneration parameters such as collagen matrix formation and angiogenesis were significantly increased in $5\mu\textrm{g}$, or $\25mu\textrm{g}$ of bFGF-treated wounds when compared to saline-treated wounds (p<0.05). pectin immunostaining on day 8 for vascular endothelium showed an increased number of neovessels in bFGF-treated wounds. These results suggest that topical application of bFGF has beneficial effects on wound healing by angiogenesis and granulation tissue formation in healing-impaired wounds.

• Toxicological Research
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• v.35 no.2
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• pp.181-190
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• 2019

Lentinus squarrosulus (Mont.) is an edible wild mushroom with tough fruiting body that belongs to the family Polyporaceae. It is used in ethnomedicine for the treatment of ulcer, anaemia, cough and fever. Recent studies have demonstrated its anticancer, anti-diabetic and antioxidant properties. However, little or no information is available regarding the bioactive components and toxicological study of wild dried L. squarrosulus. Therefore, this study investigated the bioactive components of aqueous extract of boiled wild dried L. squarrosulus and its toxicological effects in rats. The extract of L. squarrosulus was subjected to GC-MS analysis. The acute toxicity test was performed by oral administration of a single dose of up to 5,000 mg/kg extract of L. squarrosulus. In subacute study, the rats were orally administered extract of L. squarrosulus at the doses of 500, 1,000 and 1,500 mg/kg body weight daily for 14 days. The haematological, lipid profile, liver and kidney function parameters were determined and the histopathology of the liver and kidney were examined. The GC-MS analysis revealed the presence of bioactive compounds; 1-tetradecene, fumaric acid, monochloride, 6-ethyloct-3-yl ester, 9-eicosene, phytol, octahydropyrrolo[1,2-a]pyrazine and 3-trifluoroacetoxypentadecane. In acute toxicity study, neither death nor toxicity sign was recorded. In the sub-acute toxicity study, significant differences (p < 0.05) were observed on creatinine, aspartate aminotransferase, alanine aminotransferase, total cholesterol, triglycerides and high-density lipoprotein cholesterol. Whilst no significant differences (p > 0.05) were observed on packed cell volume, heamoglobin, red blood cell, white blood cell and alkaline phosphatase, in all the tested doses. No histopathological alterations were recorded. Our findings revealed that aqueous extract of L. squarrosulus may have antimicrobial, antinocieptive and antioxidant properties based on the result of GC-MS analysis. Results of the toxicity test showed no deleterious effect at the tested doses, suggesting that L. squarrosulus is safe for consumption at the tested doses.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1611-1617
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• 2004

This study was carried out to examine the effects of pills made of mulberry leaves and silkworm powder on lowering blood glucose level. Experimental animals were Sprague-Dawley male rat weighing 100$\pm$10 g and pills were supplemented with 0.4% (4 g/kg) diet. Experimental groups were assigned to diabetic group (DM group) and pill supplemented groups. Pill supplemented groups were classified 100% mulberry leaves (M group), mixing 25% silkworm powder to mulberry leaves (25SM group), mixing 50% silkworm powder to mulberry leaves (50SM group), mixing 70% silkworm powder to mulberry leaves (75SM group) and 100% silkworm powder (100S group). Experimental diets and water fed ad libitum, and streptozotocin was injected to induce diabetic state after 3rd weeks and sacrificed on the 9th day. The contents of 1-deoxynojirimycin(DNJ) were increased with adding the silkworm powder. The contents of GABA and rutin were increased with adding the mulberry leaves. In vitro, intestinal mucosa $\alpha$-glucosidase inhibitory activities were significantly increased in pills which mixed with silkworm powder by 50%. Blood glucose levels were high in groups which mixed with silkworm powder by 50% compared to DM group. Intestinal mucosa maltase activity in proximal part was significantly reduced in pill supplemented group compared to DM group and pill supplemented groups were no significant difference. Enzyme activity in middle part was no significant difference in experimental groups. Enzyme activity in distal part was decreased in pill supplemented groups, especially in 50SM, 75SM and 100S groups were significantly reduced compared to DM group. Sucrase and lactase activities in pill supplemented groups were significantly reduced at proximal part, and there was no significant difference in middle and distal parts. In conclusion, pills made of mulberry leaves and silkworm powder increased the $\alpha$-glucosidase inhibitory activity in vitro and reduced the blood glucose levels by controlling the disaccharidase activities of intestinal proximal part in STZ-induced diabetic rat. The synergistic effect was the highest when mulberry leaves was mixed with silkworm powder by the ratio of 50 : 50.

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.129-130
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• 2008

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.204-204
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• 1996

모체의 당뇨병은 신생아 호흡부전 증후군의 중요한 소인의 하나이며, 이것은 태아의 폐성숙이 지연되어 폐포를 안정화시키는 표면활성물질의 결여에 기인하는 것으로 알려져 있다. 폐의 표면활성물질은 지질과 소량의 단백으로 이루어진 복합물질로서 그 주성분은 phosphatidyl choline이며 동물의 종속에 관계없이 임신 말기에 그 양이 현저히 증가한다. 양수내 인지질은 주로 태아 폐에서 유래하므로 임신 말기의 양수내 lecithin/sphingomyelin(L/S)비 측정으로 태아의 호흡부전 증후군을 예측할 수 있다. 일반적으로 당뇨병 모체의 양수는 L/S비가 낮아 당뇨병 산모로 부터 출생한 신생아에서 호흡부전증의 발생빈도가 높으므로 이를 예방하기 위하여 부신피질호르몬의 투여가 고려되고 있다.

• Journal of Pharmacopuncture
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• v.16 no.3
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• pp.7-10
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• 2013

Objectives: This study aimed to evaluate the evidence available in the literature for the safety and efficacy of Dioscoreae Rhizoma (DR) for the treatment of peripheral neuropathy. Methods: Literature searches were performed in MEDLINE and three Korean medical databases up to April 2013. All studies evaluating the effects on peripheral neuropathy or the safety of DR monopreparations were considered. Results: Three studies - DR extract per os (po) on diabetic neuropathy in mice, DR extract injection on the peripheral sciatic nerve after crush injury in rats and DR extract injection to patients with peripheral facial paralysis proved that DR treatments were effective for the treatment of nerve injuries. Conclusions: In conclusion, we found the DR has a strong positive potential for the treatment of peripheral neuropathy, but studies addressing direct factors related to the nerve still remain insufficient.

• Natural Product Sciences
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• v.5 no.4
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• pp.155-158
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• 1999

Several biological activities of extracts from roots of Cichorium intybus Linne (Compositae) were studied in this paper. The antiinflammatory activity of the methanol extract of this root was investigated against carrageenin induced edema in rat‘s hind paw. Significant inhibitory effects were observed at the dose of 1,000 mg/kg and were compared with aspirin as a control. The hepatoprotective activities of the methanol extract, ethylacetate and butanol fraction were studied on mice whose livers are damaged by $CCl_4$. The serum transaminase activities (ALT, AST) were reduced at the dose of 1,000 mg/kg of the methanol extract, 500 mg/kg of ethylacetate and butanol fraction, respectively. The bile juice secretion was also increased significantly from each fraction. The antidiabetic activity was examined on strepto-zotocin-induced diabetic rats with methanol extract. Methanol extract gave a significant reduction of blood glucose levels in 1 week and 3 weeks.