• International Journal of Highway Engineering
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• v.12 no.3
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• pp.87-92
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• 2010

As the Concept "how environmental friendly" becomes more and more important in road construction. However, so far there is no estimation method. Environmental friendly concept can be incorporated at the plan level in order to influence decision making, and support policies that affect environment. The overall goal of this study was to develop environmental friendly concept measures for the national highway and to develop a methodology to implement a more environmental friendly concept. The research identified 8 performance measures through a project analysis that could address environmental impact assessment system's ten strategic goals - Topography, Wildlife, hydrology, landuse, air quality, water quality, soil, waste, noise, landscape. The qualitatively and quantitatively evaluation approach was selected as the decision support framework and performance measure were investigated using the AHP(Analytic Hierarchy Process) and pilot corridor for a 10 section and calculate the index values. The methodology was applied to a pilot corridor comprised of a 120km section of national highway in korea. The methodology made it possible to identify the specific performance measures that need improvement to enhance the overall environmental friendly concept. It is fairly intuitive, based on readily available data, and is easy to apply. It provides a powerful tool for government to assess the relative environmental friendly conceptof their transportation corridors now and in the future. It allows for comparisons within a corridor and with other corridors and identifies the improvements needed to enhance the environmental friendly concept.

• Journal of Korean Society on Water Environment
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• v.23 no.4
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• pp.474-481
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• 2007

With population growth, industrialization, and urbanization within the watershed, the hydrologic response changed dramatically, resulting in increases in peak flow with lesser time to peak and total runoff with shortened time of concentration. Infiltration is directly affected by initial soil moisture condition, which is a key element to determine runoff. Influence of the initial soil moisture condition on hydrograph analysis should be evaluated to assess land use change impacts on runoff and non-point source pollution characteristics. The Long-Term Hydrologic Impact Assessment (L-THIA) model has been widely used for the estimation of the direct runoff worldwide. The L-THIA model was applied to the Little Eagle Creek (LEC) watershed and Its estimated direct runoff values were compared with the BFLOW filtered direct runoff values by other researchers. The $R^2$ value Was 0.68 and the Nash-Sutcliffe coefficient value was 0.64. Also, the L-THIA estimates were compared with those separated using optimized $BFI_{max}$ value for the Eckhardt filter. The $R^2$ value and the Nash-Sutcliffe coefficient value were 0.66 and 0.63, respectively. Although these higher statistics could indicate that the L-THIA model is good in estimating the direct runoff reasonably well, the Antecedent Moisture Condition (AMC) was not adjusted in that study, which might be responsible for mismatches in peak flow between the L-THIA estimated and the measured peak values. In this study, the L-THIA model was run with AMC adjustment for direct runoff estimation. The $R^2$ value was 0.80 and the Nash-Sutcliffe coefficient value was 0.78 for the comparison of L-THIA simulated direct runoff with the filtered direct runoff. However there was 42.44% differences in the L-THIA estimated direct runoff and filtered direct runoff. This can be explained in that about 80% of the simulation period is classified as 'AMC I' condition, which caused lower CN values and lower direct runoff estimation. Thus, the coefficients of the equation to adjust CN II to CN I and CN III depending on AMC condition were modified to minimize adjustments impacts on runoff estimation. The $R^2$ and the Nash-Sutcliffe coefficient values increase, 0.80 and 0.80 respectively. The difference in the estimated and filtered direct runoff decreased from 42.44% to 7.99%. The results obtained in this study indicate the AMC needs to be considered for accurate direct runoff estimation using the L-THIA model. Also, more researches are needed for realistic adjustment of the AMC in the L-THIA model.

• Journal of Korean Society on Water Environment
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• v.29 no.6
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• pp.832-841
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• 2013

In recent years, eutrophication in the Paldang Lake has become one of the major environmental problems in Korea as it may threaten drinking water safety and human health. Thus it is important to understand the phenomena and predict the time and magnitude of algal blooms for applying adequate algal reduction measures. This study performed seasonal water quality assessment and chlorophyll-a prediction using Bayseian simple/multiple linear regression analysis. Bayseian regression analysis could be a useful tool to overcome limitations of conventional regression analysis. Also it can consider uncertainty in prediction by using posterior distribution. Generally, chlorophyll-a of a P2(Paldang Dam 2) site showed high concentration in spring and it was similar to that of P4(Paldang Dam 4) site. For the development of Bayseian model, we performed seasonal correlation. As a result, chlorophyll-a of a P2 site had a high correlation with P5(Paldang Dam 5) site in spring (r = 0.786, p<0.05) and with P4 in winter (r = 0.843, p<0.05). Based on the DIC (Deviance Information Criterion) value, critical explanatory variables of the best fitting Bayesian linear regression model were selected as a $PO_4-P$ (P2), Chlorophyll-a (P5) in spring, $NH_3-N$ (P2), Chlorophyll-a (P4), $NH_3-N$ (P4) in summer, DTP (P2), outflow (P2), TP (P3), TP (P4) fall, COD (P2), Chl-a (P4) and COD (P4) in winter. The results of chlorophyll-a prediction showed relatively high $R^2$ and low RMSE values in summer and winter.

• Korean Journal of Microbiology
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• v.34 no.4
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• pp.236-242
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• 1998

While bacteriophage P2-P4 system is very useful experimental tool for the study of viral capsid assembly, there is no useful plasmid vector for the DNA manipulation in bacteriophage P2-P4 system. In this study, a new vector plasmid, P4 ash8 (sid71) kmr, was constructed by swapping the non-essential region of P4 DNA for kanamycin resistance(kmr) gene cassette of plasmid pUC4-K. P4 ash8 sid71 was starting material for the construction, since it tends to be maintained as a plasmid in the absence of the helper phage. The total size of this chimera was designed to be packaged into P4 or P2 size heads with induction by P2 infection. The conversion of plasmid P4 ash8 (sid71) kmr to bacteriophage was proved by burst size determination experiment and CsCl buoyant equilibrium density gradient experiment. Integrase destructed P4 derivative, P4 ash8 sid71 kmr intS, was able to be constructed easily by in vitro DNA manipulation of P4 ash8 sid71 kmr. The plasmid stability experiment with P4 ash8 sid71 kmr if/tS showed that the integrase of P4 affects the stable maintenance of plasmid P4 state.

• Journal of Life Science
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• v.26 no.2
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• pp.234-240
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• 2016

The Y-maze is widely used to test working memory in behavioral science. For this purpose, spontaneous alternation behavior is monitored, and an increased percentage of spontaneous alternation is regarded as enhanced working memory. However, in some cases, the percentage of spontaneous alternation does not accurately reflect the extent of working memory in rodents. To complement the short-comings of this measure, we developed a new method to evaluate working memory on the Y-maze. This is done by defining all spontaneous alternation cases and P_i, the probability that the rodent achieved spontaneous alternation from each alternation case. After all P_i-values acquired in each animal are summarized, the result is considered as entropy. To validate the new analytical method, mice were raised under either control or an enriched environmental condition for 10 weeks, and working memory behavior on the Y-maze was monitored. The results showed that the new analytical method successfully reproduced significance. In addition, the new method turned out to be more accurate than measurement of the percentage of spontaneous alternation, meaning that, to get higher entropy, alternation should be recorded in all arms and directions. Together, these data indicate that the new analytical method is a useful supplement to the method that compares the percentage of spontaneous alternation, and thus is a good tool with which to evaluate working memory in rodents.

• Journal of Life Science
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• v.23 no.2
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• pp.290-298
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• 2013

The genus Mycobacterium includes crucial animal and human pathogens such as Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium bovis. Although it is important to understand the genetic basis for their virulence and persistence in host, genetic analysis in mycobacteria was hampered by a lack of sufficient genetic tools. Therefore, many functional vectors as molecular genetic tools have been designed for understanding mycobacterial biology, and the application of these tools to mycobacteria has accelerated the study of mechanisms involved in virulence and gene expression. To overcome the pre-existing problems in genetic manipulation of mycobacteria, this paper reports new vector systems as effective genetic tools in Mycobacterium smegmatis. Three vectors were developed; pKOTs is a suicide vector for mutagenesis containing a temperature-sensitive replication origin (TSRO) and the sacB gene encoding levansucrase as a counterselectable marker. pMV306lacZ is an integrative lacZ transcriptional fusion vector that can be inserted into chromosomal DNA by site-specific recombination. pTnMod-OKmTs is a minitransposon vector harboring the TSRO that can be used in random mutagenesis. It was demonstrated in this study that these vectors effectively worked in M. smegmatis. The vector systems reported here are expected to successfully applicable to future research of mycobacterial molecular genetics.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.1
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• pp.113-119
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• 2013

Despite rapid changes in the structure of industry, manufacturing remains a key industry for economic progress, promotion of trade, increased employment, and the creation of new industries. Production technologies are essential for strengthening the competitiveness of small- and medium-sized manufacturing industries. However, it is very difficult to standardize and systematically propagate production technology from an experienced worker to an inexperienced worker because these technologies are generally improved by the skilled people in a workshop. In this study, we introduce a Web-based platform system consisting of a knowledge authoring tool, technology database, semantic database, and Web portal service for sharing production technologies for the exterior housing parts of mobile products. By investigating various cellular phone designs, reference form factors for three types of mobile phone housings were designed based on the standard features. In addition, several manufacturing technologies and considerable information such as reference mold designs and molding conditions optimized using CAE and recent R&D outputs are stored in this system.

• Journal of the Korean Society for Nondestructive Testing
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• v.24 no.4
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• pp.390-395
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• 2004

The iSTMS is a web-based integrated information system supporting Pre-Service and In-Service Inspection(PSI/ISI) processes for the nuclear power plants of KHNP(Korea Hydro & Nuclear Power Co. Ltd.). The system provides a full spectrum coverage of the inspection processes from the planning stage to the final report of examination in accordance with applicable codes, standards, and regulatory requirements. The major functions of the system includes the inspection planning, examination, reporting, project control and status reporting, resource management as well as objects search and navigation. The system also provides two dimensional or three dimensional visualization interface to identify the location and geometry of components and weld areas subject to examination in collaboration with database applications. The iSIMS is implemented with commercial software packages such as database management system, 2-D and 3-D visualization tool, etc., which provide open, updated and verified foundations. This paper describes the key functions and the technologies for the implementation of the iSIMS.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.10 no.3
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• pp.145-153
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• 2005

I reviewed an ecological and environmental significance of microbial carbon respiration coupled to dis-similatory reduction of fe(III) to Fe(II) which is one of the major processes controlling mineralization of organic matter and behavior of metals and nutrients in various anaerobic environments. Relative significance of Fe(III) reduction in the mineralization of organic matter in diverse marine environments appeared to be extremely variable, ranging from negligible up to $100\%$. Cenerally, Fe(III) reduction dominated anaerobic car-bon mineralization when concentrations of reactive Fe(III) were higher, indicating that availability of reactive Fe(III) was a major factor determining the relative significance of Fe(III) reduction in anaerobic carbon mineralization. In anaerobic coastal sediments where $O_2$ supply is limited, tidal flushing, bioturbation and vegetation were most likely responsible for regulating the availability of Fe(III) for Fe(III) reducing bacteria (FeRB). Capabilities of FeRB in mineralization of organic matter and conversion of metals implied that FeRB may function as a useful eco-technological tool for the bioremediation of anoxic coastal environments contaminated by toxic organic and metal pollutants.

• Korean Journal of Food Science and Technology
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• v.46 no.3
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• pp.269-275
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• 2014

We developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) for determining the buckwheat content in processed foods by using rabbit polyclonal antibodies against buckwheat proteins (BWP). The detection limit of this assay was $0.05-100{\mu}g/mL$. The cross-reactivities of the anti-BWP antibodies toward BWP, buckwheat flour, whole buckwheat, and cereals (wheat flour, whole wheat, black bean, mung bean, red bean, brack rice, brown rice, glutinous rice, white rice, millet, African millet, nonglutinous millet, adlay, and rye) were 100, 17.9, 11.8, and 0%, respectively. Thus, the antibodies were found to be specific for buckwheat only. When buckwheat flour was heated for 30 min, the mean assay recoveries of BWP were 83.0% at $60-90^{\circ}C$ and 44.5% at $100^{\circ}C$. The spike test showed that the mean assay recoveries of buckwheat from raw noodle, boiled noodle, starch gel, and cereal flour were 99.1, 98.6, 81.1, and 104%, respectively. For the 22 commercial items tested, the qualitative coincidence ratio of assay result and the corresponding value indicated on the item's package label was 100%. However, the average quantitative coincidence ratios from 12 commercial items were 31.6%. Thus, the results suggest that ciELISA is an efficient tool to detect buckwheat in processed foods.